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The map label for this gene is hisS [H]

Identifier: 94311043

GI number: 94311043

Start: 2286272

End: 2287615

Strand: Reverse

Name: hisS [H]

Synonym: Rmet_2105

Alternate gene names: 94311043

Gene position: 2287615-2286272 (Counterclockwise)

Preceding gene: 94311044

Following gene: 94311042

Centisome position: 58.24

GC content: 62.43

Gene sequence:

>1344_bases
ATGAGCGAAGACAAGACGAAGGCCGTCAAGGCACTGCAGGGCGTGAAGGGCATGAACGACATGCTGCCCACTGACGCCCC
GCTGTGGGATATGTTCGAAAGCGCGGCGCGCAGCATGTTGCGCGCCTATGGCTACCAGCAGATCCGCACGCCGATCGTCG
AGCATACCCAGCTGTTCGTGCGCGGTATCGGCGAGGTCACCGACATCGTCGAGAAGGAGATGTACTCCTTCACCGATTCC
CTGAACGGCGAGAATCTGACGCTGCGTCCGGAGGGCACTGCTGCGGCCGTGCGTTCGACGATCGAGCATAACCTGCTGTA
TGACGGCCCCAAGCGCCTCTGGTACATCGGCCCGATGTTCCGTCACGAACGCCCGCAGCGTGGTCGCTATCGTCAGTTCC
ACCAGCTCGGCGCGGAAGCGCTCGGCTTTGCCGGCCCGGACGTCGACGCGGAAATCATCCTGATGTGCCAGCGCCTGTGG
GACGACCTCGGGCTGACCAACGTCCGTCTCGAACTGAACTCGCTGGGCCAGGCGCATGAGCGCGCCGCGCATCGCGAGCA
GCTCATCAAGTACCTGGAAGGCTTCCAGGACATCCTCGATGAGGACAGCAAGCGTCGTCTGTACACGAACCCGCTGCGGG
TGCTGGACACGAAGAATCCGGCGCTGCAGGAGATGGCCGCCAATGCACCGAAGCTGATCGACTTCCTCGGGGAGGAGTCG
CTCGCTCACTTCGAGGGCGTTCAGAGGATCCTGCTGGCCAACAATATTCCGTTCAAGATCAACCCGCGTCTGGTGCGCGG
CCTGGACTACTACAACCTGACCGTGTTCGAGTGGATCACGGACAAGCTGGGTGCCCAGGGCACGATCGCCGGCGGTGGCC
GTTATGACCCGCTGATCCAGCAGATGGGTGGCAAGCCTGCGCCGGCCTGCGGCTGGGCAATGGGCATCGAGCGGATCATC
GAACTGATCCGCGAAGAGAACCTGGTGCCGGATACGCAGGGCTGTGACGTCTACATGGTTCACCAGGGTGAATTGGCGAA
CCAGCAGGCAATGATCGCTGCCGAACGCCTGCGTGACGCCGGGCTGGACGTGGTCCTCCATGCCACGCCGGACGGCAAGA
GCGGGAGCTTCAAGTCGCAGATGAAGCGTGCCGATGCAAGTGGCGCATCCTTTGCAGTTATCATTGGCGACGACGAAATC
GCCGCGGGCACGGTGCAGGTCAAGCAACTGCGCGGCGGCAATGTGGCCGAAGGTGGCCAGCAAACCACTGTGCCGGCCGA
GCAGGTTGTCGAATTCATCATCGACGCGATGGTCGATGAGATCGAAGGCGACGACGAGCAATAA

Upstream 100 bases:

>100_bases
CTACGGTCCGGACGCGAAACAGGCTTCACAAGAAGCGGTGGCCTGATCGATTCGGCGCCATCGCGCAAGGATCGGGCATC
ACCGTAACAACAAATCAGAC

Downstream 100 bases:

>100_bases
TCTGCCGTTCCCTATCGCAGCACCCGGTCGTTGTCCCTGACGGCGGCCGGTTATATTCAACCTAAGCAGATAACTGCCTC
GACATGGCTTACGATCTAGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSEDKTKAVKALQGVKGMNDMLPTDAPLWDMFESAARSMLRAYGYQQIRTPIVEHTQLFVRGIGEVTDIVEKEMYSFTDS
LNGENLTLRPEGTAAAVRSTIEHNLLYDGPKRLWYIGPMFRHERPQRGRYRQFHQLGAEALGFAGPDVDAEIILMCQRLW
DDLGLTNVRLELNSLGQAHERAAHREQLIKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAANAPKLIDFLGEES
LAHFEGVQRILLANNIPFKINPRLVRGLDYYNLTVFEWITDKLGAQGTIAGGGRYDPLIQQMGGKPAPACGWAMGIERII
ELIREENLVPDTQGCDVYMVHQGELANQQAMIAAERLRDAGLDVVLHATPDGKSGSFKSQMKRADASGASFAVIIGDDEI
AAGTVQVKQLRGGNVAEGGQQTTVPAEQVVEFIIDAMVDEIEGDDEQ

Sequences:

>Translated_447_residues
MSEDKTKAVKALQGVKGMNDMLPTDAPLWDMFESAARSMLRAYGYQQIRTPIVEHTQLFVRGIGEVTDIVEKEMYSFTDS
LNGENLTLRPEGTAAAVRSTIEHNLLYDGPKRLWYIGPMFRHERPQRGRYRQFHQLGAEALGFAGPDVDAEIILMCQRLW
DDLGLTNVRLELNSLGQAHERAAHREQLIKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAANAPKLIDFLGEES
LAHFEGVQRILLANNIPFKINPRLVRGLDYYNLTVFEWITDKLGAQGTIAGGGRYDPLIQQMGGKPAPACGWAMGIERII
ELIREENLVPDTQGCDVYMVHQGELANQQAMIAAERLRDAGLDVVLHATPDGKSGSFKSQMKRADASGASFAVIIGDDEI
AAGTVQVKQLRGGNVAEGGQQTTVPAEQVVEFIIDAMVDEIEGDDEQ
>Mature_446_residues
SEDKTKAVKALQGVKGMNDMLPTDAPLWDMFESAARSMLRAYGYQQIRTPIVEHTQLFVRGIGEVTDIVEKEMYSFTDSL
NGENLTLRPEGTAAAVRSTIEHNLLYDGPKRLWYIGPMFRHERPQRGRYRQFHQLGAEALGFAGPDVDAEIILMCQRLWD
DLGLTNVRLELNSLGQAHERAAHREQLIKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAANAPKLIDFLGEESL
AHFEGVQRILLANNIPFKINPRLVRGLDYYNLTVFEWITDKLGAQGTIAGGGRYDPLIQQMGGKPAPACGWAMGIERIIE
LIREENLVPDTQGCDVYMVHQGELANQQAMIAAERLRDAGLDVVLHATPDGKSGSFKSQMKRADASGASFAVIIGDDEIA
AGTVQVKQLRGGNVAEGGQQTTVPAEQVVEFIIDAMVDEIEGDDEQ

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=52.5943396226415, Blast_Score=447, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 49696; Mature: 49565

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDKTKAVKALQGVKGMNDMLPTDAPLWDMFESAARSMLRAYGYQQIRTPIVEHTQLFV
CCCHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RGIGEVTDIVEKEMYSFTDSLNGENLTLRPEGTAAAVRSTIEHNLLYDGPKRLWYIGPMF
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCEECCCCCEEEECHHH
RHERPQRGRYRQFHQLGAEALGFAGPDVDAEIILMCQRLWDDLGLTNVRLELNSLGQAHE
HCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCHHHH
RAAHREQLIKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAANAPKLIDFLGEES
HHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHEEECCCHHHHHHHCCCHHHHHHHCHHH
LAHFEGVQRILLANNIPFKINPRLVRGLDYYNLTVFEWITDKLGAQGTIAGGGRYDPLIQ
HHHHHHHHHHHHHCCCCEEECHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHH
QMGGKPAPACGWAMGIERIIELIREENLVPDTQGCDVYMVHQGELANQQAMIAAERLRDA
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHC
GLDVVLHATPDGKSGSFKSQMKRADASGASFAVIIGDDEIAAGTVQVKQLRGGNVAEGGQ
CCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCC
QTTVPAEQVVEFIIDAMVDEIEGDDEQ
CCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEDKTKAVKALQGVKGMNDMLPTDAPLWDMFESAARSMLRAYGYQQIRTPIVEHTQLFV
CCHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RGIGEVTDIVEKEMYSFTDSLNGENLTLRPEGTAAAVRSTIEHNLLYDGPKRLWYIGPMF
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCEECCCCCEEEECHHH
RHERPQRGRYRQFHQLGAEALGFAGPDVDAEIILMCQRLWDDLGLTNVRLELNSLGQAHE
HCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCHHHH
RAAHREQLIKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAANAPKLIDFLGEES
HHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHEEECCCHHHHHHHCCCHHHHHHHCHHH
LAHFEGVQRILLANNIPFKINPRLVRGLDYYNLTVFEWITDKLGAQGTIAGGGRYDPLIQ
HHHHHHHHHHHHHCCCCEEECHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHH
QMGGKPAPACGWAMGIERIIELIREENLVPDTQGCDVYMVHQGELANQQAMIAAERLRDA
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHC
GLDVVLHATPDGKSGSFKSQMKRADASGASFAVIIGDDEIAAGTVQVKQLRGGNVAEGGQ
CCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCC
QTTVPAEQVVEFIIDAMVDEIEGDDEQ
CCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA