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The map label for this gene is rnhB

Identifier: 94310391

GI number: 94310391

Start: 1570802

End: 1571482

Strand: Direct

Name: rnhB

Synonym: Rmet_1449

Alternate gene names: 94310391

Gene position: 1570802-1571482 (Clockwise)

Preceding gene: 94310390

Following gene: 94310392

Centisome position: 39.99

GC content: 60.65

Gene sequence:

>681_bases
ATGGCGCGAACGAAAGCTGCGGCTTCTAGCCAGTTGGGCCTGGAACTAACTCAAACCGTTGCTGTGGTGCAGCGCTTGTG
TGGGGTAGATGAGGCTGGGCGTGGCCCCTTGGCGGGTCCCGTTTACGCTGCGGCCGTGGTATTAAATCCCGCGCGGCAAA
TCCGTGGCTTGGCCGACTCGAAAATCCTCAGTGCGGAAAAACGCGAGGCACTGTTCGATCGCATCTGCGACCACGCGCTG
GGATGGCATATCGCATCCGCGTCGGTTGAGGAGATCGATTCGATCAATATCCTCCACGCGTCGATGCTTGCAATGCAACG
GGCGGTGCAGGGCTTGGCCGCGAGCGGCGTCATTCCCGATCTCGTGCAGGTCGATGGTAATCGTTGTCCGCAGGTAGCGT
TCCCGGTCGAGGCTATCGTCAAGGGCGATGCCAAGGTACGGGCAATTTCCGCGGCTTCGATCCTGGCGAAGGTGGCGCGC
GATCGTGAACTGTTGAAGCTGCACCAGGAGTACCCTGAGTACGGGTTCGACTCTCACTTTGGCTATCCGACGCCACAGCA
TTTCACCGCGCTCGAACAATTCGGCGCGACGCCGCATCATCGGCGTTCGTTTGCGCCGGTGCGCAAGGTGCTTGAGCGTG
GCATGGTACGCACCATGTCGTCCGAAGCGCCGCCTTTCTGA

Upstream 100 bases:

>100_bases
GGCAGCATTTCACGACGATGCACGAAACCCTGAAGCGCGACACGGCGAAGCTGGCTGCCGGCGTAGTCGTCGATCTGATG
CGCAGCCGTGGAGTGGTCTG

Downstream 100 bases:

>100_bases
GGCAGACTCTTGAAGCATGTCACTTCGCGCGACAACGCGCTGTTCAAACAGTTAAAAGCCCTGACAGGGTCCACGCACCA
GCGCCGCAAGGCGGGGCAGT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MARTKAAASSQLGLELTQTVAVVQRLCGVDEAGRGPLAGPVYAAAVVLNPARQIRGLADSKILSAEKREALFDRICDHAL
GWHIASASVEEIDSINILHASMLAMQRAVQGLAASGVIPDLVQVDGNRCPQVAFPVEAIVKGDAKVRAISAASILAKVAR
DRELLKLHQEYPEYGFDSHFGYPTPQHFTALEQFGATPHHRRSFAPVRKVLERGMVRTMSSEAPPF

Sequences:

>Translated_226_residues
MARTKAAASSQLGLELTQTVAVVQRLCGVDEAGRGPLAGPVYAAAVVLNPARQIRGLADSKILSAEKREALFDRICDHAL
GWHIASASVEEIDSINILHASMLAMQRAVQGLAASGVIPDLVQVDGNRCPQVAFPVEAIVKGDAKVRAISAASILAKVAR
DRELLKLHQEYPEYGFDSHFGYPTPQHFTALEQFGATPHHRRSFAPVRKVLERGMVRTMSSEAPPF
>Mature_225_residues
ARTKAAASSQLGLELTQTVAVVQRLCGVDEAGRGPLAGPVYAAAVVLNPARQIRGLADSKILSAEKREALFDRICDHALG
WHIASASVEEIDSINILHASMLAMQRAVQGLAASGVIPDLVQVDGNRCPQVAFPVEAIVKGDAKVRAISAASILAKVARD
RELLKLHQEYPEYGFDSHFGYPTPQHFTALEQFGATPHHRRSFAPVRKVLERGMVRTMSSEAPPF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=189, Percent_Identity=55.5555555555556, Blast_Score=207, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_RALME (Q1LNE4)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583601.1
- ProteinModelPortal:   Q1LNE4
- SMR:   Q1LNE4
- STRING:   Q1LNE4
- GeneID:   4038252
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1449
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q1LNE4
- ProtClustDB:   PRK00015
- BioCyc:   RMET266264:RMET_1449-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 24398; Mature: 24267

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTKAAASSQLGLELTQTVAVVQRLCGVDEAGRGPLAGPVYAAAVVLNPARQIRGLADS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
KILSAEKREALFDRICDHALGWHIASASVEEIDSINILHASMLAMQRAVQGLAASGVIPD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LVQVDGNRCPQVAFPVEAIVKGDAKVRAISAASILAKVARDRELLKLHQEYPEYGFDSHF
HHHCCCCCCCCHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GYPTPQHFTALEQFGATPHHRRSFAPVRKVLERGMVRTMSSEAPPF
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARTKAAASSQLGLELTQTVAVVQRLCGVDEAGRGPLAGPVYAAAVVLNPARQIRGLADS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
KILSAEKREALFDRICDHALGWHIASASVEEIDSINILHASMLAMQRAVQGLAASGVIPD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LVQVDGNRCPQVAFPVEAIVKGDAKVRAISAASILAKVARDRELLKLHQEYPEYGFDSHF
HHHCCCCCCCCHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GYPTPQHFTALEQFGATPHHRRSFAPVRKVLERGMVRTMSSEAPPF
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA