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The map label for this gene is rsuA1 [H]

Identifier: 94310374

GI number: 94310374

Start: 1550581

End: 1552413

Strand: Reverse

Name: rsuA1 [H]

Synonym: Rmet_1432

Alternate gene names: 94310374

Gene position: 1552413-1550581 (Counterclockwise)

Preceding gene: 94310375

Following gene: 94310372

Centisome position: 39.52

GC content: 68.69

Gene sequence:

>1833_bases
ATGACCGATAGCAATACGCCCGGCCGCAAGACGCTGGGCATCAAGACCACCTCCTCCAGCACAACGCGCAAGGCGAGCCG
CCCGGTGCGCGTGTCTGACCTGAACCGCAAGCGTGTCCAGGAAGTGGTCACGGGGATCAAGCAGGCCAAGGACCGCGCCT
CCGACAAGGACAAGCGCAAGGCTGGGCCGGCCGATGGCGCGCCGCGCAATGAGCGTCAGGACCGACCGGCGCGACCGGCA
CGTCCGCGCCCCGCCGAAGGCGAGCGGGCACCTCGCCGTTTTGCCGAAGGAGAGCGGGCACCACGCCGGTTCAGCGATGA
GGAGCGCCCGCAGCGCGAGCGCCGGTACGGCGATGACAACCGCCCCGCGCAACCGCGCCGCTATGGCGATGACAATCGAG
CCGAGCGTCCGCGTCGGTTCAGCGACGAAGATCGTGCCGCACGCCCCCGCCGCTTTGGCGACGAGGAACGTCCGCAGCGT
GAACGCCGCTATGGGGATGACAACCGCCCCGCGCAACCGCGTCGCTATGGCGATGACAATCGCGCCGAGCGTCCGCGTCG
ATTCAGCGACGAGAATCGCGCCGCTTCGCCCCGCCGCTTTGGCGACGAGGAACGTCCGCAACGCGAGCGGCGCTACGGAG
ATGACAACCGCCCCGCGCAACCGCGTCGCTATGGCGATGACAATCGCGCCGAGCGTCCGCGTCGATTCAGCGACGAGAAT
CGCGCCGCCCCGCCCCGCCGCTTTGGCGACGAGGAACGCCCGCAGCGTGAACGCCGCGCTGGAGACGACAACCGCTCCGC
GCAACCGCGCCGCTATGGCGACGACAATCGCGCCGAACGTCCCCGCCGCTACGGCGATGACGAGCGCACGCAGCGTACCG
AGCGCCCGCACCGCAGCGACGAAGCTCGCGCGCCGCGCCGGCAGCACCAGGACGACAACGCACCGCGTCGTGGTGACGCC
CCGGCACGCCGCTTCAGCGATTCGGCCGACCGCGCCCGTGGTGAACGTACCGAACGCCCGGTGCCGCGCGCCGAAGCGCG
TCAGCCGGTCTCCGAGCATGAAGACGGCCTCGTCCGTCTCTCCAAGCGCATGTCCGAACTGGGCCTGTGCTCGCGCCGCG
AGGCCGATGAATGGATTCCGCGCGGCTGGGTGCTCGTCGACGGTAAGCCAGTGACCGAACTCGGCACGCGCATTCGTCCC
GAAGCGGAGATCGAAATCCTGCAGGCCGCGCGCTCGGAACAAGGCGAACGCGTGACGGTGCTGCTTAACAAGCCGGTCGG
CTATGTCTCCGGCCAGGCCGAAGACGGCTATGAGCCGGCTGCTGTGCTGTTTGCGCCTGAGAACCAGTGGGAACTGGACC
CCACCCGCAAGCGCTTCGCCCCGTGGCAACGCAAGAATCTCGCGCCGGCTGGCCGGCTCGACATCGACTCGACCGGGCTG
CTCGTGCTGACCCAGGATGGCCGCGTTGCACGCGCACTGATCGGCGACGATTCGAATGTCGAGAAGGAATATCTGGTGCG
CGTCGAGTGGGAATCGCCGCAGGGTCTGGTCGAGCGCAATATCAGTGCCGTGTTCCCCGACGACCTGCTGGAGCGTCTGC
GCCACGGTCTGTCGCTGGACGGCGTGGCGCTCAAGCCGGCCAAGGTCAGCTGGCAGAACGAGGAGCAACTGCGCTTCGTG
CTGCGTGAAGGCCGCAAGCGTCAGATTCGCCGGATGTGCGAGCAGGTTGGCCTGAAGGTGGTTGGCCTCAAGCGCGTGCG
CATGGGCCGTGTGGTGCTCGGCGACCTGCCGCCGGGCCAGTGGCGCTTCCTGGGGGCCTTCGAGAAGTTCTGA

Upstream 100 bases:

>100_bases
CTCGCGACTGGCCGCCGACAACCGATTGCAGATTCAGCTTGAACAGGACCTGCTCGACGCGCTGGCCATCTAAGGCCGGC
ACGCCGGTAGTTGAAACGAT

Downstream 100 bases:

>100_bases
CAGGGGCGCCTGCCGGCTATTCCTCTCGTCCGTCAGCGGGAGGGAGGAATAGCCATCAATCACTCAAATGCCTGCGCTCT
GCCCGGTCACCGGCCGCAGC

Product: 16S rRNA uridine-516 pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MTDSNTPGRKTLGIKTTSSSTTRKASRPVRVSDLNRKRVQEVVTGIKQAKDRASDKDKRKAGPADGAPRNERQDRPARPA
RPRPAEGERAPRRFAEGERAPRRFSDEERPQRERRYGDDNRPAQPRRYGDDNRAERPRRFSDEDRAARPRRFGDEERPQR
ERRYGDDNRPAQPRRYGDDNRAERPRRFSDENRAASPRRFGDEERPQRERRYGDDNRPAQPRRYGDDNRAERPRRFSDEN
RAAPPRRFGDEERPQRERRAGDDNRSAQPRRYGDDNRAERPRRYGDDERTQRTERPHRSDEARAPRRQHQDDNAPRRGDA
PARRFSDSADRARGERTERPVPRAEARQPVSEHEDGLVRLSKRMSELGLCSRREADEWIPRGWVLVDGKPVTELGTRIRP
EAEIEILQAARSEQGERVTVLLNKPVGYVSGQAEDGYEPAAVLFAPENQWELDPTRKRFAPWQRKNLAPAGRLDIDSTGL
LVLTQDGRVARALIGDDSNVEKEYLVRVEWESPQGLVERNISAVFPDDLLERLRHGLSLDGVALKPAKVSWQNEEQLRFV
LREGRKRQIRRMCEQVGLKVVGLKRVRMGRVVLGDLPPGQWRFLGAFEKF

Sequences:

>Translated_610_residues
MTDSNTPGRKTLGIKTTSSSTTRKASRPVRVSDLNRKRVQEVVTGIKQAKDRASDKDKRKAGPADGAPRNERQDRPARPA
RPRPAEGERAPRRFAEGERAPRRFSDEERPQRERRYGDDNRPAQPRRYGDDNRAERPRRFSDEDRAARPRRFGDEERPQR
ERRYGDDNRPAQPRRYGDDNRAERPRRFSDENRAASPRRFGDEERPQRERRYGDDNRPAQPRRYGDDNRAERPRRFSDEN
RAAPPRRFGDEERPQRERRAGDDNRSAQPRRYGDDNRAERPRRYGDDERTQRTERPHRSDEARAPRRQHQDDNAPRRGDA
PARRFSDSADRARGERTERPVPRAEARQPVSEHEDGLVRLSKRMSELGLCSRREADEWIPRGWVLVDGKPVTELGTRIRP
EAEIEILQAARSEQGERVTVLLNKPVGYVSGQAEDGYEPAAVLFAPENQWELDPTRKRFAPWQRKNLAPAGRLDIDSTGL
LVLTQDGRVARALIGDDSNVEKEYLVRVEWESPQGLVERNISAVFPDDLLERLRHGLSLDGVALKPAKVSWQNEEQLRFV
LREGRKRQIRRMCEQVGLKVVGLKRVRMGRVVLGDLPPGQWRFLGAFEKF
>Mature_609_residues
TDSNTPGRKTLGIKTTSSSTTRKASRPVRVSDLNRKRVQEVVTGIKQAKDRASDKDKRKAGPADGAPRNERQDRPARPAR
PRPAEGERAPRRFAEGERAPRRFSDEERPQRERRYGDDNRPAQPRRYGDDNRAERPRRFSDEDRAARPRRFGDEERPQRE
RRYGDDNRPAQPRRYGDDNRAERPRRFSDENRAASPRRFGDEERPQRERRYGDDNRPAQPRRYGDDNRAERPRRFSDENR
AAPPRRFGDEERPQRERRAGDDNRSAQPRRYGDDNRAERPRRYGDDERTQRTERPHRSDEARAPRRQHQDDNAPRRGDAP
ARRFSDSADRARGERTERPVPRAEARQPVSEHEDGLVRLSKRMSELGLCSRREADEWIPRGWVLVDGKPVTELGTRIRPE
AEIEILQAARSEQGERVTVLLNKPVGYVSGQAEDGYEPAAVLFAPENQWELDPTRKRFAPWQRKNLAPAGRLDIDSTGLL
VLTQDGRVARALIGDDSNVEKEYLVRVEWESPQGLVERNISAVFPDDLLERLRHGLSLDGVALKPAKVSWQNEEQLRFVL
REGRKRQIRRMCEQVGLKVVGLKRVRMGRVVLGDLPPGQWRFLGAFEKF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=251, Percent_Identity=35.8565737051793, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788510, Length=248, Percent_Identity=31.8548387096774, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787524, Length=264, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081838, Length=167, Percent_Identity=33.5329341317365, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 70828; Mature: 70697

Theoretical pI: Translated: 11.07; Mature: 11.07

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSNTPGRKTLGIKTTSSSTTRKASRPVRVSDLNRKRVQEVVTGIKQAKDRASDKDKRK
CCCCCCCCCEEEEEEECCCCHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCHHHHH
AGPADGAPRNERQDRPARPARPRPAEGERAPRRFAEGERAPRRFSDEERPQRERRYGDDN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
RPAQPRRYGDDNRAERPRRFSDEDRAARPRRFGDEERPQRERRYGDDNRPAQPRRYGDDN
CCCCCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC
RAERPRRFSDENRAASPRRFGDEERPQRERRYGDDNRPAQPRRYGDDNRAERPRRFSDEN
HHCCCCCCCCCCCCCCCHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
RAAPPRRFGDEERPQRERRAGDDNRSAQPRRYGDDNRAERPRRYGDDERTQRTERPHRSD
CCCCCHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHCCHHCCCHHHHHHHCCCCCCC
EARAPRRQHQDDNAPRRGDAPARRFSDSADRARGERTERPVPRAEARQPVSEHEDGLVRL
HHCCCHHHCCCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHH
SKRMSELGLCSRREADEWIPRGWVLVDGKPVTELGTRIRPEAEIEILQAARSEQGERVTV
HHHHHHHCCHHCCCHHHHCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHHCCCCEEEE
LLNKPVGYVSGQAEDGYEPAAVLFAPENQWELDPTRKRFAPWQRKNLAPAGRLDIDSTGL
EEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCE
LVLTQDGRVARALIGDDSNVEKEYLVRVEWESPQGLVERNISAVFPDDLLERLRHGLSLD
EEEECCCCEEEHHHCCCCCCCCEEEEEEECCCCHHHHHCCCHHCCCHHHHHHHHHCCCCC
GVALKPAKVSWQNEEQLRFVLREGRKRQIRRMCEQVGLKVVGLKRVRMGRVVLGDLPPGQ
CEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHCCEEECCCCCCC
WRFLGAFEKF
EEECCHHHCC
>Mature Secondary Structure 
TDSNTPGRKTLGIKTTSSSTTRKASRPVRVSDLNRKRVQEVVTGIKQAKDRASDKDKRK
CCCCCCCCEEEEEEECCCCHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCHHHHH
AGPADGAPRNERQDRPARPARPRPAEGERAPRRFAEGERAPRRFSDEERPQRERRYGDDN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
RPAQPRRYGDDNRAERPRRFSDEDRAARPRRFGDEERPQRERRYGDDNRPAQPRRYGDDN
CCCCCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC
RAERPRRFSDENRAASPRRFGDEERPQRERRYGDDNRPAQPRRYGDDNRAERPRRFSDEN
HHCCCCCCCCCCCCCCCHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
RAAPPRRFGDEERPQRERRAGDDNRSAQPRRYGDDNRAERPRRYGDDERTQRTERPHRSD
CCCCCHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHCCHHCCCHHHHHHHCCCCCCC
EARAPRRQHQDDNAPRRGDAPARRFSDSADRARGERTERPVPRAEARQPVSEHEDGLVRL
HHCCCHHHCCCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHH
SKRMSELGLCSRREADEWIPRGWVLVDGKPVTELGTRIRPEAEIEILQAARSEQGERVTV
HHHHHHHCCHHCCCHHHHCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHHCCCCEEEE
LLNKPVGYVSGQAEDGYEPAAVLFAPENQWELDPTRKRFAPWQRKNLAPAGRLDIDSTGL
EEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCE
LVLTQDGRVARALIGDDSNVEKEYLVRVEWESPQGLVERNISAVFPDDLLERLRHGLSLD
EEEECCCCEEEHHHCCCCCCCCEEEEEEECCCCHHHHHCCCHHCCCHHHHHHHHHCCCCC
GVALKPAKVSWQNEEQLRFVLREGRKRQIRRMCEQVGLKVVGLKRVRMGRVVLGDLPPGQ
CEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHCCEEECCCCCCC
WRFLGAFEKF
EEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]