Click here to switch to the map view.

The map label for this gene is actII-2 [H]

Identifier: 94310372

GI number: 94310372

Start: 1548044

End: 1549486

Strand: Reverse

Name: actII-2 [H]

Synonym: Rmet_1430

Alternate gene names: 94310372

Gene position: 1549486-1548044 (Counterclockwise)

Preceding gene: 94310374

Following gene: 94310367

Centisome position: 39.45

GC content: 66.81

Gene sequence:

>1443_bases
ATGCAAACATCCCCCGCCCCCACCCTCGGCCGAGCCGGACTTTACGTCCTGCTGGCCGGCCAGCTACTGCCACAGATCGA
CTTTTCGATCGTCAATGTGGCGCTACACCCTATCGAGCACTCCCTGGGAGCGACGCCGTCCGAACTGGAACTGATGGTGG
CCGTCTACGGCGTGGCATTCGCCGTCTTCCTGGCCATGGGCGGGCGGCTCGGCGACAACTACGGCCGCCGTCGCATCTTC
AACATCGGCGTGCTGCTCTTCGGCGTCGCGTCGCTACTGTGCGGCGTGGCGGGTTCGATGCTGTCGCTGCTGATTGCGCG
GACGCTGCAAGGCGCGGCGGCGGCGCTGCTCGTGCCGCAGATCCTGGCGACAATTCACGTCGGCCTGCGCGGACACGCTC
ACTCCCGTGCCCTGGCGTTGTTCGCGTCGATTGGCGGCATCGCGTTCATCATCGGGCAGGTTCTGGGCGGCTTGCTGGTC
GATGCCGACATCGACGGCCTGGGATGGCGCAACGCGTTCCTCATCAACCTGCCAATCTGCCTGGCCATCCTGGTCCTTTC
GCCGCGCGTGATCCCCGAGACCCGGCGTGAGAACGCCACTGGCATCGATGCGCCAGGCACCGTGCTGCTGGCAGCCGTGA
TCCTGGCAATTCTGCTTCCGATCGCGTTGGGACCCATGTTTCGCTGGTCGTGGCCCGTGCTGGCCGTTCTGGCCGCCACG
GCGCCGTTGATGCTGCTGCTCTGGTACATCGAACTGCGCCAGGAACGCCGCGGCGTGCATCCGCTGTTGCCGCCGTCGCT
GCTGCGACTGCCAAGCGTGCGCTTCGGTTTTATCGTCGCCGTGCTGTTCTATGCGTGCTGGAGCGGCCTGATGTTCGTGC
TGGCGCTAACGCTGCAGAGCGGCGCCGGTATGACGCCCACGATGGCGGGCAACGCATTCATTCCGCTTGGGCTGGCCTTT
ATTGCGGGATCGCTGACGAGCCCGCGCGTGGTGAACGGCTGGGGCCGCACGCGTGTGCTGATTGTCGGATGCGTGACACA
GATGGCCGGCGTTGTGGTGCTGGCCTACGTGCTGCGGCAAGCCTGGCCCCACCCGGACATCCTGACGCTCGCCCCGCCAA
GCTTGCTGATCGGCTTCGGCAATGCGCTGGTGGTCGGGTGCTTCTACCGGATTGGCCTGGCGGACATCCCCACCGACCAG
GCCGGCGCGGGCAGCGCCGCGCTGGCCACGGTTCAGCAGGCCTCGCTCGGGCTCGGCTCGGCCCTGCTCGGCACGGTATT
CGCGCAGCGACTGCATCACGCCGCGACTTATCTCGATGCGGCGCTGGCCAGCCTCGCCGCCGAGTTGTGCCTGATGACGA
TCCTGGTTGCCGCTGCGCTGGTCTATCACAACCGGCATGGGCAGCCGGCAATCACGGCGTGCGCCTCGCCTCAGTCGTCG
TAA

Upstream 100 bases:

>100_bases
CTAGGCTGACACAATTTATACCAGTCTAACTGGACTCTTGTACCAGTCTGATCGGGCCACCATACTCCGGTCCTCAAGCG
TTGTCGAGGCCAGATCCGCC

Downstream 100 bases:

>100_bases
TTGGGTGGTAGTTCGGGGAACAGGATCTCGGTAAAACCGAAGCGCGTGAAGTCCTTGATCCGCATCGGATACAGCACGCC
GATCAGGTGATCGCACTCGT

Product: putative MFS transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MQTSPAPTLGRAGLYVLLAGQLLPQIDFSIVNVALHPIEHSLGATPSELELMVAVYGVAFAVFLAMGGRLGDNYGRRRIF
NIGVLLFGVASLLCGVAGSMLSLLIARTLQGAAAALLVPQILATIHVGLRGHAHSRALALFASIGGIAFIIGQVLGGLLV
DADIDGLGWRNAFLINLPICLAILVLSPRVIPETRRENATGIDAPGTVLLAAVILAILLPIALGPMFRWSWPVLAVLAAT
APLMLLLWYIELRQERRGVHPLLPPSLLRLPSVRFGFIVAVLFYACWSGLMFVLALTLQSGAGMTPTMAGNAFIPLGLAF
IAGSLTSPRVVNGWGRTRVLIVGCVTQMAGVVVLAYVLRQAWPHPDILTLAPPSLLIGFGNALVVGCFYRIGLADIPTDQ
AGAGSAALATVQQASLGLGSALLGTVFAQRLHHAATYLDAALASLAAELCLMTILVAAALVYHNRHGQPAITACASPQSS

Sequences:

>Translated_480_residues
MQTSPAPTLGRAGLYVLLAGQLLPQIDFSIVNVALHPIEHSLGATPSELELMVAVYGVAFAVFLAMGGRLGDNYGRRRIF
NIGVLLFGVASLLCGVAGSMLSLLIARTLQGAAAALLVPQILATIHVGLRGHAHSRALALFASIGGIAFIIGQVLGGLLV
DADIDGLGWRNAFLINLPICLAILVLSPRVIPETRRENATGIDAPGTVLLAAVILAILLPIALGPMFRWSWPVLAVLAAT
APLMLLLWYIELRQERRGVHPLLPPSLLRLPSVRFGFIVAVLFYACWSGLMFVLALTLQSGAGMTPTMAGNAFIPLGLAF
IAGSLTSPRVVNGWGRTRVLIVGCVTQMAGVVVLAYVLRQAWPHPDILTLAPPSLLIGFGNALVVGCFYRIGLADIPTDQ
AGAGSAALATVQQASLGLGSALLGTVFAQRLHHAATYLDAALASLAAELCLMTILVAAALVYHNRHGQPAITACASPQSS
>Mature_480_residues
MQTSPAPTLGRAGLYVLLAGQLLPQIDFSIVNVALHPIEHSLGATPSELELMVAVYGVAFAVFLAMGGRLGDNYGRRRIF
NIGVLLFGVASLLCGVAGSMLSLLIARTLQGAAAALLVPQILATIHVGLRGHAHSRALALFASIGGIAFIIGQVLGGLLV
DADIDGLGWRNAFLINLPICLAILVLSPRVIPETRRENATGIDAPGTVLLAAVILAILLPIALGPMFRWSWPVLAVLAAT
APLMLLLWYIELRQERRGVHPLLPPSLLRLPSVRFGFIVAVLFYACWSGLMFVLALTLQSGAGMTPTMAGNAFIPLGLAF
IAGSLTSPRVVNGWGRTRVLIVGCVTQMAGVVVLAYVLRQAWPHPDILTLAPPSLLIGFGNALVVGCFYRIGLADIPTDQ
AGAGSAALATVQQASLGLGSALLGTVFAQRLHHAATYLDAALASLAAELCLMTILVAAALVYHNRHGQPAITACASPQSS

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=462, Percent_Identity=27.2727272727273, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788392, Length=425, Percent_Identity=27.5294117647059, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789042, Length=420, Percent_Identity=26.4285714285714, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788710, Length=452, Percent_Identity=25, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081983, Length=295, Percent_Identity=27.4576271186441, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324954, Length=486, Percent_Identity=22.6337448559671, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50206; Mature: 50206

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSPAPTLGRAGLYVLLAGQLLPQIDFSIVNVALHPIEHSLGATPSELELMVAVYGVAF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AVFLAMGGRLGDNYGRRRIFNIGVLLFGVASLLCGVAGSMLSLLIARTLQGAAAALLVPQ
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILATIHVGLRGHAHSRALALFASIGGIAFIIGQVLGGLLVDADIDGLGWRNAFLINLPIC
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEHHHHH
LAILVLSPRVIPETRRENATGIDAPGTVLLAAVILAILLPIALGPMFRWSWPVLAVLAAT
HHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
APLMLLLWYIELRQERRGVHPLLPPSLLRLPSVRFGFIVAVLFYACWSGLMFVLALTLQS
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAGMTPTMAGNAFIPLGLAFIAGSLTSPRVVNGWGRTRVLIVGCVTQMAGVVVLAYVLRQ
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AWPHPDILTLAPPSLLIGFGNALVVGCFYRIGLADIPTDQAGAGSAALATVQQASLGLGS
HCCCCCEEEECCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHH
ALLGTVFAQRLHHAATYLDAALASLAAELCLMTILVAAALVYHNRHGQPAITACASPQSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MQTSPAPTLGRAGLYVLLAGQLLPQIDFSIVNVALHPIEHSLGATPSELELMVAVYGVAF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AVFLAMGGRLGDNYGRRRIFNIGVLLFGVASLLCGVAGSMLSLLIARTLQGAAAALLVPQ
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILATIHVGLRGHAHSRALALFASIGGIAFIIGQVLGGLLVDADIDGLGWRNAFLINLPIC
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEHHHHH
LAILVLSPRVIPETRRENATGIDAPGTVLLAAVILAILLPIALGPMFRWSWPVLAVLAAT
HHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
APLMLLLWYIELRQERRGVHPLLPPSLLRLPSVRFGFIVAVLFYACWSGLMFVLALTLQS
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAGMTPTMAGNAFIPLGLAFIAGSLTSPRVVNGWGRTRVLIVGCVTQMAGVVVLAYVLRQ
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AWPHPDILTLAPPSLLIGFGNALVVGCFYRIGLADIPTDQAGAGSAALATVQQASLGLGS
HCCCCCEEEECCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHH
ALLGTVFAQRLHHAATYLDAALASLAAELCLMTILVAAALVYHNRHGQPAITACASPQSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]