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The map label for this gene is phaA [H]

Identifier: 94310299

GI number: 94310299

Start: 1466344

End: 1467525

Strand: Direct

Name: phaA [H]

Synonym: Rmet_1357

Alternate gene names: 94310299

Gene position: 1466344-1467525 (Clockwise)

Preceding gene: 94310298

Following gene: 94310300

Centisome position: 37.33

GC content: 65.65

Gene sequence:

>1182_bases
ATGACCGATATCGTCATCGTTTCCGCAGCCCGTACCGCAGTTGGCAAGTTCGGCGGTTCGCTCGCGAAGATTCCCGCGCC
GGAGTTGGGCGCCATCGTGATCAAGGCCGCGCTGGAGCGTGCCGGCGTGAAGCCGGACCAGGTGAGCGAAGTGATCATGG
GCCAGGTGCTGACGGCCGCCTCGGGTCAGAACCCGGCACGCCAGGCTTCGCGCAAGGCGGGCCTGCCCGACATGGTGCCG
GCCATGACCATCAACAAGGTGTGCGGTTCGGGCCTGAAAGCCGTGATGCTGGCCGCCAACGCGATTGTGGCTGGCGAGGC
CGAGATCGTCGTGGCCGGCGGCATGGAGAACATGAGCGCCGCGCCGCACGTGCTGCCGGGTTCGCGCGATGGCTTCCGCA
TGGGCGACACCAAGCTGGTCGATTCGATGATCGTTGACGGCCTGTGGGATGTGTACAACCAGTACCACATGGGTATCACG
GCCGAGAACGTGGCCAAGGAATACGGCATCACCCGCCAGGCCCAGGACGAGTTCGCCGTGGGCTCCCAGAACAAGGCCGA
AGCCGCCCAGAAGGCCGGCAAGTTCGACGAGGAAATCGTTCCGGTCCTGATTCCGCAACGCAAGGGCGATCCGGTTGCGT
TCAAGACCGACGAGTACGTGCGTCAGGGCGCCACGCTGGAAAGCATGGCTAGCCTGAAACCGGCCTTCGACAAGACCGGC
ACGGTGACGGCCGCCAACGCATCGGGCCTGAATGATGGCGCCGCCGCTGTGGTCGTGATGTCGGCGGCCAAGGCGCGCGA
ACTGGGCCTGACCCCGCTGGCGACCATCAAGGCGTACGCCAACGCCGGCGTGGACCCGGCCGTGATGGGCATGGGCCCGG
TGCCTGCCAGCAAGCGCTGCCTGTCGCGTGCCGGCTGGGAGGTCAAGGACCTGGACCTGATGGAAATCAACGAGGCCTTT
GCCGCTCAGGCACTGGCCGTGCATCAGCAAATGGGCTGGGATCAGTCGAAGATCAACGTGAACGGCGGCGCGATCGCCAT
CGGCCATCCGATCGGCGCGTCAGGCTGCCGCATCCTGGTCACGCTGCTGCACGAAATGAAGCGCCGCGATGCCAAGAAGG
GCCTGGCCTCGCTGTGCATCGGTGGTGGTATGGGCGTGGCGCTCGCAGTCGAGCGGAATTAA

Upstream 100 bases:

>100_bases
CGCTACGTCAAACAGCGCGCCTGAGCTTCATAAGGGCCGGCCCGCGACGCCGGCCCGGATTTCGAACCGTTCCGCATTCG
TTTTCTCGAAAGGACATCAA

Downstream 100 bases:

>100_bases
GCATCGCAATTGCATTGAGGGGTGTGCCGGCCGTGTACGTCCAGGACGGATGCGGCCGGCGTGAATAAGAAAGACCAAAG
CTTCGACACGATCGGGCGAG

Product: putative acyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTDIVIVSAARTAVGKFGGSLAKIPAPELGAIVIKAALERAGVKPDQVSEVIMGQVLTAASGQNPARQASRKAGLPDMVP
AMTINKVCGSGLKAVMLAANAIVAGEAEIVVAGGMENMSAAPHVLPGSRDGFRMGDTKLVDSMIVDGLWDVYNQYHMGIT
AENVAKEYGITRQAQDEFAVGSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEYVRQGATLESMASLKPAFDKTG
TVTAANASGLNDGAAAVVVMSAAKARELGLTPLATIKAYANAGVDPAVMGMGPVPASKRCLSRAGWEVKDLDLMEINEAF
AAQALAVHQQMGWDQSKINVNGGAIAIGHPIGASGCRILVTLLHEMKRRDAKKGLASLCIGGGMGVALAVERN

Sequences:

>Translated_393_residues
MTDIVIVSAARTAVGKFGGSLAKIPAPELGAIVIKAALERAGVKPDQVSEVIMGQVLTAASGQNPARQASRKAGLPDMVP
AMTINKVCGSGLKAVMLAANAIVAGEAEIVVAGGMENMSAAPHVLPGSRDGFRMGDTKLVDSMIVDGLWDVYNQYHMGIT
AENVAKEYGITRQAQDEFAVGSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEYVRQGATLESMASLKPAFDKTG
TVTAANASGLNDGAAAVVVMSAAKARELGLTPLATIKAYANAGVDPAVMGMGPVPASKRCLSRAGWEVKDLDLMEINEAF
AAQALAVHQQMGWDQSKINVNGGAIAIGHPIGASGCRILVTLLHEMKRRDAKKGLASLCIGGGMGVALAVERN
>Mature_392_residues
TDIVIVSAARTAVGKFGGSLAKIPAPELGAIVIKAALERAGVKPDQVSEVIMGQVLTAASGQNPARQASRKAGLPDMVPA
MTINKVCGSGLKAVMLAANAIVAGEAEIVVAGGMENMSAAPHVLPGSRDGFRMGDTKLVDSMIVDGLWDVYNQYHMGITA
ENVAKEYGITRQAQDEFAVGSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEYVRQGATLESMASLKPAFDKTGT
VTAANASGLNDGAAAVVVMSAAKARELGLTPLATIKAYANAGVDPAVMGMGPVPASKRCLSRAGWEVKDLDLMEINEAFA
AQALAVHQQMGWDQSKINVNGGAIAIGHPIGASGCRILVTLLHEMKRRDAKKGLASLCIGGGMGVALAVERN

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=56.7774936061381, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=45.0381679389313, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI167614485, Length=390, Percent_Identity=44.3589743589744, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=41.0126582278481, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=30.8962264150943, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=30.690537084399, Blast_Score=155, Evalue=4e-38,
Organism=Escherichia coli, GI87082165, Length=393, Percent_Identity=60.559796437659, Blast_Score=489, Evalue=1e-140,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=60.3053435114504, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=42.75, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=41.3533834586466, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1788683, Length=409, Percent_Identity=35.2078239608802, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=46.1538461538462, Blast_Score=337, Evalue=9e-93,
Organism=Caenorhabditis elegans, GI25147385, Length=387, Percent_Identity=43.1524547803618, Blast_Score=308, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17535921, Length=397, Percent_Identity=42.5692695214106, Blast_Score=283, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI17535917, Length=393, Percent_Identity=34.3511450381679, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=33.1775700934579, Blast_Score=195, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6325229, Length=397, Percent_Identity=43.3249370277078, Blast_Score=304, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=37.6590330788804, Blast_Score=227, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=57.1428571428571, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24640423, Length=393, Percent_Identity=45.5470737913486, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI17648125, Length=390, Percent_Identity=45.1282051282051, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=31.0588235294118, Blast_Score=181, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40727; Mature: 40595

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00154 ATPASE_E1_E2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIVIVSAARTAVGKFGGSLAKIPAPELGAIVIKAALERAGVKPDQVSEVIMGQVLTAA
CCCEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
SGQNPARQASRKAGLPDMVPAMTINKVCGSGLKAVMLAANAIVAGEAEIVVAGGMENMSA
CCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHEEECCCEEEEECCCCCCCC
APHVLPGSRDGFRMGDTKLVDSMIVDGLWDVYNQYHMGITAENVAKEYGITRQAQDEFAV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHCC
GSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEYVRQGATLESMASLKPAFDKTG
CCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCEECCHHHHHCCCCHHHHHHCCCCCCCCC
TVTAANASGLNDGAAAVVVMSAAKARELGLTPLATIKAYANAGVDPAVMGMGPVPASKRC
CEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCCCHHHHH
LSRAGWEVKDLDLMEINEAFAAQALAVHQQMGWDQSKINVNGGAIAIGHPIGASGCRILV
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCHHHHHH
TLLHEMKRRDAKKGLASLCIGGGMGVALAVERN
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
TDIVIVSAARTAVGKFGGSLAKIPAPELGAIVIKAALERAGVKPDQVSEVIMGQVLTAA
CCEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
SGQNPARQASRKAGLPDMVPAMTINKVCGSGLKAVMLAANAIVAGEAEIVVAGGMENMSA
CCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHEEECCCEEEEECCCCCCCC
APHVLPGSRDGFRMGDTKLVDSMIVDGLWDVYNQYHMGITAENVAKEYGITRQAQDEFAV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHCC
GSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEYVRQGATLESMASLKPAFDKTG
CCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCEECCHHHHHCCCCHHHHHHCCCCCCCCC
TVTAANASGLNDGAAAVVVMSAAKARELGLTPLATIKAYANAGVDPAVMGMGPVPASKRC
CEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCCCHHHHH
LSRAGWEVKDLDLMEINEAFAAQALAVHQQMGWDQSKINVNGGAIAIGHPIGASGCRILV
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCHHHHHH
TLLHEMKRRDAKKGLASLCIGGGMGVALAVERN
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2670935; 2670936 [H]