Click here to switch to the map view.

The map label for this gene is phaC1 [H]

Identifier: 94310298

GI number: 94310298

Start: 1464177

End: 1466267

Strand: Direct

Name: phaC1 [H]

Synonym: Rmet_1356

Alternate gene names: 94310298

Gene position: 1464177-1466267 (Clockwise)

Preceding gene: 94310296

Following gene: 94310299

Centisome position: 37.27

GC content: 63.27

Gene sequence:

>2091_bases
ATGCCTTTAAATGTATCGAAACCGAGGAATTACTCTAGGGTTTCGCCGGCTAGCATAGCGTATGACGCAGGGCATCCGAG
GTCGTTCTGCAGCAGTGTCAGACGCAACCCGGATCAGGGGCGGGTCGGGAGACAGTCACCCCGAGCCCGTCAACCAATAC
GACGGCAGAGAGACGAATACATTATGGCGACCGGCAAAGGTGCGGCAGCTTCCGCTAAGGAAGGCAAGTCCCAACCGTTT
CAGTTCGTGCCGGGGCCATTTGATCCAGCCACCTGGCTGGAGTGGTCCCGTCAATGGGTACCCGAGGCCAACGGCGATAT
GCCGTCAGGTTTCCCGGCCGGTATGGCCGGCGCGTTTCCGGGTGGATTCCCGGGCGGCGAGGCATTCGTCCAGGCCTTCC
AGAACGCTTTCCAGGGTGCCACCGCGCAGGGTGCGAACGGCATGAATGGCGCCGGCGGCGTGAAGATCGCGCCCGCGCAG
CTTGCTCGTATCCAGGAACGCTTTTTCAGGGATTTCGCCGAACTCTGGCGAGAAATGGGCGAGGGCGCCGTGGCAGCCAG
CACGCCTGCGCCCAACGGCGCGGCCAATGCCGATTCAGCCAGCCAGCGCTTGTCCGACAAGCGTTTCGCCAGCCCGGCAT
GGCGCAACAATGCCCCGTATCGCTATACCGCAGCCTTTTACCTGCTGACGGCCCGGGCCATGACCGAACTGGCCGAGGCG
GTCGAGGCCGATCCCAAGGTTCGTCAGCGCATCCGCTTCGCGGTATCGCAATGGGTGGATGCCATGTCGCCGGCCAACTT
CCTGGCGACGAACCCCGACGCGCAACAGCGCCTGATCGAATCCGGTGGTGAGTCGCTGCGCAAGGGCGTGCGCAATATGC
TGGAGGATCTGGGCAGGGGCAAGATCTCACAGACTGACGAAACAGCGTTCGAGGTCGGCCGCAATATGGCCGTGACCGAA
GGCGCCGTAGTCTACGAGAACGAGTATTTCCAGCTTCTGCAGTACAAGCCTCTGACCGACAAGGTCTACGAGCGGCCGCT
GCTGATGGTGCCGCCGTGCATCAACAAGTTCTACATTCTGGACCTGCAACCCGAGAGCTCGCTGGTGCGTCATGCGGTGG
AGCAGGGGAATACCGTCTTCCTGGTGTCGTGGCGCAACCCCGATGCATCGATGGCCGAGCGCACCTGGGATGAGTACATC
GAGCACGCGGCGATCCGCGCGATCGAAGTGACGCGCGACATCAGCGGACAGGACAAGATCAACGTGCTGGGCTTCTGCGT
CGGCGGCACGATCGTCTCGACGGCGCTTTCGGTGCTGGCCGCGCGTGGCGAGCGTCCCGCAGCCAGCCTGACATTGCTGA
CCACGCTGCTGGATTTCGCCGATACGGGCATCCTCGATGTCTTCGTCGACGAAGCGCACGTGCAACTGCGCGAGGCCACG
CTGGGTGGGGCCGGCGGCGCGCCGTGCGGGCTGCTGCGCGGCGTGGAACTTGCCAATACGTTCTCGTTCCTTCGTCCGAA
CGACCTGGTCTGGAACTACGTGGTCGACAACTATCTCAAGGGCAACACGCCGGTTCCGTTCGACCTGTTGTTCTGGAATG
GCGACGCCACCAATCTCCCGGGCCCGTGGTACTGCTGGTACCTGAGGCATACGTATCTGCAGGACGAGTTGAAGGTGCCG
GGCAAGCTCACGGTCTGCAACGCGCCGGTGGACTTCGGGGCGATCGACGTGCCAACGTACATCTACGGGTCGCGCGAAGA
CCACATCGTGCCCTGGGCCGCGGCCTACGCTTCCACGGCGCTGCTGCGGAACAAGTTGCGTTTCGTGCTGGGTGCCTCTG
GCCATATCGCGGGCGTCATCAACCCGCCGGCCAAGAAGAAGCGCAGTCATTGGACCAATGACAAATTGTCGGAATCGGCC
CATGATTGGCTGGCCGGCGCGCAGGAGCATCCGGGAAGCTGGTGGCCGGACTGGGTGACATGGCTGGGCAAGCAGGCGGG
TCAGAAGCGTGCCGCGCCAGCCGATTACGGCAATGACCACTACCGGGCGATTGAACCCGCGCCAGGGCGCTACGTCAAAC
AGCGCGCCTGA

Upstream 100 bases:

>100_bases
CCGCGCCAGGCCTACCGTCCGGTAGGTCGGGATTTGGCGGCCAGGGAGTTACCTGTCGTGCGGATTTCCGCATTGACAGC
GTCTACATTGCGAGGCAACA

Downstream 100 bases:

>100_bases
GCTTCATAAGGGCCGGCCCGCGACGCCGGCCCGGATTTCGAACCGTTCCGCATTCGTTTTCTCGAAAGGACATCAAATGA
CCGATATCGTCATCGTTTCC

Product: Poly(3-hydroxybutyrate) polymerase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MPLNVSKPRNYSRVSPASIAYDAGHPRSFCSSVRRNPDQGRVGRQSPRARQPIRRQRDEYIMATGKGAAASAKEGKSQPF
QFVPGPFDPATWLEWSRQWVPEANGDMPSGFPAGMAGAFPGGFPGGEAFVQAFQNAFQGATAQGANGMNGAGGVKIAPAQ
LARIQERFFRDFAELWREMGEGAVAASTPAPNGAANADSASQRLSDKRFASPAWRNNAPYRYTAAFYLLTARAMTELAEA
VEADPKVRQRIRFAVSQWVDAMSPANFLATNPDAQQRLIESGGESLRKGVRNMLEDLGRGKISQTDETAFEVGRNMAVTE
GAVVYENEYFQLLQYKPLTDKVYERPLLMVPPCINKFYILDLQPESSLVRHAVEQGNTVFLVSWRNPDASMAERTWDEYI
EHAAIRAIEVTRDISGQDKINVLGFCVGGTIVSTALSVLAARGERPAASLTLLTTLLDFADTGILDVFVDEAHVQLREAT
LGGAGGAPCGLLRGVELANTFSFLRPNDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLPGPWYCWYLRHTYLQDELKVP
GKLTVCNAPVDFGAIDVPTYIYGSREDHIVPWAAAYASTALLRNKLRFVLGASGHIAGVINPPAKKKRSHWTNDKLSESA
HDWLAGAQEHPGSWWPDWVTWLGKQAGQKRAAPADYGNDHYRAIEPAPGRYVKQRA

Sequences:

>Translated_696_residues
MPLNVSKPRNYSRVSPASIAYDAGHPRSFCSSVRRNPDQGRVGRQSPRARQPIRRQRDEYIMATGKGAAASAKEGKSQPF
QFVPGPFDPATWLEWSRQWVPEANGDMPSGFPAGMAGAFPGGFPGGEAFVQAFQNAFQGATAQGANGMNGAGGVKIAPAQ
LARIQERFFRDFAELWREMGEGAVAASTPAPNGAANADSASQRLSDKRFASPAWRNNAPYRYTAAFYLLTARAMTELAEA
VEADPKVRQRIRFAVSQWVDAMSPANFLATNPDAQQRLIESGGESLRKGVRNMLEDLGRGKISQTDETAFEVGRNMAVTE
GAVVYENEYFQLLQYKPLTDKVYERPLLMVPPCINKFYILDLQPESSLVRHAVEQGNTVFLVSWRNPDASMAERTWDEYI
EHAAIRAIEVTRDISGQDKINVLGFCVGGTIVSTALSVLAARGERPAASLTLLTTLLDFADTGILDVFVDEAHVQLREAT
LGGAGGAPCGLLRGVELANTFSFLRPNDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLPGPWYCWYLRHTYLQDELKVP
GKLTVCNAPVDFGAIDVPTYIYGSREDHIVPWAAAYASTALLRNKLRFVLGASGHIAGVINPPAKKKRSHWTNDKLSESA
HDWLAGAQEHPGSWWPDWVTWLGKQAGQKRAAPADYGNDHYRAIEPAPGRYVKQRA
>Mature_695_residues
PLNVSKPRNYSRVSPASIAYDAGHPRSFCSSVRRNPDQGRVGRQSPRARQPIRRQRDEYIMATGKGAAASAKEGKSQPFQ
FVPGPFDPATWLEWSRQWVPEANGDMPSGFPAGMAGAFPGGFPGGEAFVQAFQNAFQGATAQGANGMNGAGGVKIAPAQL
ARIQERFFRDFAELWREMGEGAVAASTPAPNGAANADSASQRLSDKRFASPAWRNNAPYRYTAAFYLLTARAMTELAEAV
EADPKVRQRIRFAVSQWVDAMSPANFLATNPDAQQRLIESGGESLRKGVRNMLEDLGRGKISQTDETAFEVGRNMAVTEG
AVVYENEYFQLLQYKPLTDKVYERPLLMVPPCINKFYILDLQPESSLVRHAVEQGNTVFLVSWRNPDASMAERTWDEYIE
HAAIRAIEVTRDISGQDKINVLGFCVGGTIVSTALSVLAARGERPAASLTLLTTLLDFADTGILDVFVDEAHVQLREATL
GGAGGAPCGLLRGVELANTFSFLRPNDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLPGPWYCWYLRHTYLQDELKVPG
KLTVCNAPVDFGAIDVPTYIYGSREDHIVPWAAAYASTALLRNKLRFVLGASGHIAGVINPPAKKKRSHWTNDKLSESAH
DWLAGAQEHPGSWWPDWVTWLGKQAGQKRAAPADYGNDHYRAIEPAPGRYVKQRA

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 76556; Mature: 76425

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNVSKPRNYSRVSPASIAYDAGHPRSFCSSVRRNPDQGRVGRQSPRARQPIRRQRDEY
CCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCE
IMATGKGAAASAKEGKSQPFQFVPGPFDPATWLEWSRQWVPEANGDMPSGFPAGMAGAFP
EEEECCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GGFPGGEAFVQAFQNAFQGATAQGANGMNGAGGVKIAPAQLARIQERFFRDFAELWREMG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHC
EGAVAASTPAPNGAANADSASQRLSDKRFASPAWRNNAPYRYTAAFYLLTARAMTELAEA
CCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
VEADPKVRQRIRFAVSQWVDAMSPANFLATNPDAQQRLIESGGESLRKGVRNMLEDLGRG
HCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCC
KISQTDETAFEVGRNMAVTEGAVVYENEYFQLLQYKPLTDKVYERPLLMVPPCINKFYIL
CCCCCHHHHHHHCCCEEEECCEEEECCHHHHHHHCCCCHHHHHHCCCEECCCCCCCEEEE
DLQPESSLVRHAVEQGNTVFLVSWRNPDASMAERTWDEYIEHAAIRAIEVTRDISGQDKI
EECCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
NVLGFCVGGTIVSTALSVLAARGERPAASLTLLTTLLDFADTGILDVFVDEAHVQLREAT
EEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHH
LGGAGGAPCGLLRGVELANTFSFLRPNDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLP
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
GPWYCWYLRHTYLQDELKVPGKLTVCNAPVDFGAIDVPTYIYGSREDHIVPWAAAYASTA
CCEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHH
LLRNKLRFVLGASGHIAGVINPPAKKKRSHWTNDKLSESAHDWLAGAQEHPGSWWPDWVT
HHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
WLGKQAGQKRAAPADYGNDHYRAIEPAPGRYVKQRA
HHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHCCCC
>Mature Secondary Structure 
PLNVSKPRNYSRVSPASIAYDAGHPRSFCSSVRRNPDQGRVGRQSPRARQPIRRQRDEY
CCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCE
IMATGKGAAASAKEGKSQPFQFVPGPFDPATWLEWSRQWVPEANGDMPSGFPAGMAGAFP
EEEECCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GGFPGGEAFVQAFQNAFQGATAQGANGMNGAGGVKIAPAQLARIQERFFRDFAELWREMG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHC
EGAVAASTPAPNGAANADSASQRLSDKRFASPAWRNNAPYRYTAAFYLLTARAMTELAEA
CCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
VEADPKVRQRIRFAVSQWVDAMSPANFLATNPDAQQRLIESGGESLRKGVRNMLEDLGRG
HCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCC
KISQTDETAFEVGRNMAVTEGAVVYENEYFQLLQYKPLTDKVYERPLLMVPPCINKFYIL
CCCCCHHHHHHHCCCEEEECCEEEECCHHHHHHHCCCCHHHHHHCCCEECCCCCCCEEEE
DLQPESSLVRHAVEQGNTVFLVSWRNPDASMAERTWDEYIEHAAIRAIEVTRDISGQDKI
EECCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
NVLGFCVGGTIVSTALSVLAARGERPAASLTLLTTLLDFADTGILDVFVDEAHVQLREAT
EEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHH
LGGAGGAPCGLLRGVELANTFSFLRPNDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLP
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
GPWYCWYLRHTYLQDELKVPGKLTVCNAPVDFGAIDVPTYIYGSREDHIVPWAAAYASTA
CCEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHH
LLRNKLRFVLGASGHIAGVINPPAKKKRSHWTNDKLSESAHDWLAGAQEHPGSWWPDWVT
HHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
WLGKQAGQKRAAPADYGNDHYRAIEPAPGRYVKQRA
HHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2670936; 1987116 [H]