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The map label for this gene is ykgE [H]

Identifier: 94310151

GI number: 94310151

Start: 1325608

End: 1326333

Strand: Direct

Name: ykgE [H]

Synonym: Rmet_1206

Alternate gene names: 94310151

Gene position: 1325608-1326333 (Clockwise)

Preceding gene: 94310149

Following gene: 94310152

Centisome position: 33.75

GC content: 63.22

Gene sequence:

>726_bases
ATGCGAATCGGACTGTTCGCCACCTGTCTGGTGGACCTCATGCGCCCCGAGATCGGGTTCTCGGTGCTGAAGCTGCTGGA
ATCGGCTGGCTACGAGGTCATGGTGCCCGAGGCGCAGACCTGCTGCGGACAGCCTGCCTACAACTCGGGTGAGCGCGCTG
TATCACGTGACCTGGCGGAGAAATTCCTGCGCGAGTTCGAGATGTTCGACTACGTGGTGGTGCCGTCTGGCTCCTGCGGC
GGCATGATTCGCCAGCACTATCAGGACCTGCTGCGCGACGATCCGGAACTGCATGGCCGCTACGAGCGCCTGCGGGAGCG
CGTCTTCGAACTGACCGATTTCCTCGTCAACGTGGCAAAGATTGACCGGTTCGATTCCACGTTTGCCGGCCGTGTCACCT
ATCACGATTCCTGTTCCGGGCTGCGCGAGCTGGGCGTCAAGCAGCAGCCACGCGAGCTGCTGGGCAAGCTGCCGGGCGTG
ACGCTGACCGAAATGAAGGACTGCGAGGCGTGCTGCGGATTCGGCGGCACGTTCTCGGTCAAGTACGGCAACATCTCCAC
GGCGATCGTCGACGAGAAGTGCGCGAATATTCAGTCCAGCGGGGCCGATGCCGTGGTGCTCGGTGACCTCGGCTGTATCC
TCAACATCGAAGGCCGGCTGCGCCGCACGGGCGATACGCGCACACGTGTGCTGCATATTGCCCAGGTGCTGGCTGGCGAC
GCCTGA

Upstream 100 bases:

>100_bases
ATTGTGCGGCGGGATGAACAAGATTGCGGCGCCGGATGCCCGCAGCGGCGGGGGCAAGGGGCATAATTGCGATGTTTCTG
GGTCTGGAAAAAGGGAAGCG

Downstream 100 bases:

>100_bases
AGACCCGCAACGAGCCCCCACGATGCAAGTCCACAGCATGGAATTCAAGGCGCGCGCAGGGCAGAAGCTTGCCGACCAGC
GCCTGCAGCAGAACCTCAAG

Product: putative hydroxyacid oxidoreductase (Fe-S centre)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MRIGLFATCLVDLMRPEIGFSVLKLLESAGYEVMVPEAQTCCGQPAYNSGERAVSRDLAEKFLREFEMFDYVVVPSGSCG
GMIRQHYQDLLRDDPELHGRYERLRERVFELTDFLVNVAKIDRFDSTFAGRVTYHDSCSGLRELGVKQQPRELLGKLPGV
TLTEMKDCEACCGFGGTFSVKYGNISTAIVDEKCANIQSSGADAVVLGDLGCILNIEGRLRRTGDTRTRVLHIAQVLAGD
A

Sequences:

>Translated_241_residues
MRIGLFATCLVDLMRPEIGFSVLKLLESAGYEVMVPEAQTCCGQPAYNSGERAVSRDLAEKFLREFEMFDYVVVPSGSCG
GMIRQHYQDLLRDDPELHGRYERLRERVFELTDFLVNVAKIDRFDSTFAGRVTYHDSCSGLRELGVKQQPRELLGKLPGV
TLTEMKDCEACCGFGGTFSVKYGNISTAIVDEKCANIQSSGADAVVLGDLGCILNIEGRLRRTGDTRTRVLHIAQVLAGD
A
>Mature_241_residues
MRIGLFATCLVDLMRPEIGFSVLKLLESAGYEVMVPEAQTCCGQPAYNSGERAVSRDLAEKFLREFEMFDYVVVPSGSCG
GMIRQHYQDLLRDDPELHGRYERLRERVFELTDFLVNVAKIDRFDSTFAGRVTYHDSCSGLRELGVKQQPRELLGKLPGV
TLTEMKDCEACCGFGGTFSVKYGNISTAIVDEKCANIQSSGADAVVLGDLGCILNIEGRLRRTGDTRTRVLHIAQVLAGD
A

Specific function: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source [H]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lutA/ykgE family [H]

Homologues:

Organism=Escherichia coli, GI1786497, Length=238, Percent_Identity=37.8151260504202, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI48994913, Length=241, Percent_Identity=21.1618257261411, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004017
- InterPro:   IPR022822 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 26645; Mature: 26645

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGLFATCLVDLMRPEIGFSVLKLLESAGYEVMVPEAQTCCGQPAYNSGERAVSRDLAE
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCHHHHHHHHHH
KFLREFEMFDYVVVPSGSCGGMIRQHYQDLLRDDPELHGRYERLRERVFELTDFLVNVAK
HHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IDRFDSTFAGRVTYHDSCSGLRELGVKQQPRELLGKLPGVTLTEMKDCEACCGFGGTFSV
HHHHCCCCCCEEEECHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEE
KYGNISTAIVDEKCANIQSSGADAVVLGDLGCILNIEGRLRRTGDTRTRVLHIAQVLAGD
EECCEEHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCEECCCCHHHHHHHHHHHHHCC
A
C
>Mature Secondary Structure
MRIGLFATCLVDLMRPEIGFSVLKLLESAGYEVMVPEAQTCCGQPAYNSGERAVSRDLAE
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCHHHHHHHHHH
KFLREFEMFDYVVVPSGSCGGMIRQHYQDLLRDDPELHGRYERLRERVFELTDFLVNVAK
HHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IDRFDSTFAGRVTYHDSCSGLRELGVKQQPRELLGKLPGVTLTEMKDCEACCGFGGTFSV
HHHHCCCCCCEEEECHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEE
KYGNISTAIVDEKCANIQSSGADAVVLGDLGCILNIEGRLRRTGDTRTRVLHIAQVLAGD
EECCEEHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCEECCCCHHHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA