Click here to switch to the map view.

The map label for this gene is folD

Identifier: 94310137

GI number: 94310137

Start: 1306839

End: 1307696

Strand: Reverse

Name: folD

Synonym: Rmet_1192

Alternate gene names: 94310137

Gene position: 1307696-1306839 (Counterclockwise)

Preceding gene: 94310139

Following gene: 94310136

Centisome position: 33.29

GC content: 66.43

Gene sequence:

>858_bases
ATGTCCGCTCACCTGATCGACGGTAACGCCCTTGCCAAACAAATCCGCGCCGAGGCCGCCCAGCGCGCTGCCCGCCTGAC
CGCGGCCGGCCACCAGCCCGGCCTGGCCGTGGTGCTCGTGGGCGAGGACCCCGCCAGCCAGGTCTATGTGCGCAACAAGG
TGAAGGCGTGCGAGGACAACGGATTCTATTCGTCGCTGAACCGCTACCCGGCCGACCTGTCCGAAGCCGACCTGCTGGCC
CGCATCGACGAACTGAACAACGACCCGAAGATCCACGGGATCCTGGTCCAACTGCCGCTGCCCAAGCATATCGACAGCCA
CAAGGTGCTCGAAGCCATCGCCCCGGAAAAGGACGTCGACGGCTTCCACGTGGCCAACGCCGGCGCGCTGATGACCGGCG
CCCCCCTGTTCCGCCCCTGCACGCCGTACGGCTGCATGAAGATGCTCGAATCGATCCAGTACCCGCTGCGCGGCGCGCGT
GCCGTGGTGGTGGGTGCCTCGAACATCGTCGGCAAGCCGATGGCCATGATGCTGCTGCAGGCTGGCGCAACCGTAACGAT
CTGCAATAGCAAGACGCGCGACCTCGCCGCCCATACGCGCGACGCCGACGTGATCGTCGCTGCCGTGGGTCGCCGCAACA
TCATTACTGCCGACATCGTCAAGCCGGGCGCCGTGGTGATCGACGTAGGCATGAACCGTGACGACGCCGGCAAGCTGTGC
GGCGATGTGGACTTCGCCGGTGTGCGCGAAGTGGCCGGCCATATCACCCCGGTTCCGGGTGGCGTCGGCCCGATGACGAT
CACGATGCTGCTGATCAACACGCTGGAAGCCGCGGAACGCGAAGCCGGCATCGCCTGA

Upstream 100 bases:

>100_bases
TCTGATCAACCCCTTTATAATCCGGGTTTCACGAGCGACGCTGGCGACCCCCGCCGGCGTCGCTCCCCGTTTCCCCAAGC
CTCTCCAGTCACGTATCCCC

Downstream 100 bases:

>100_bases
GTGATGCTCTAGGTGATGGCCTGAGCGATGGGCCAATTGCGCCAATAGCTAAGTCGTACTGGATATTTCGGCGCATAGGC
CTCATCTTGCACGTGTGATG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSAHLIDGNALAKQIRAEAAQRAARLTAAGHQPGLAVVLVGEDPASQVYVRNKVKACEDNGFYSSLNRYPADLSEADLLA
RIDELNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESIQYPLRGAR
AVVVGASNIVGKPMAMMLLQAGATVTICNSKTRDLAAHTRDADVIVAAVGRRNIITADIVKPGAVVIDVGMNRDDAGKLC
GDVDFAGVREVAGHITPVPGGVGPMTITMLLINTLEAAEREAGIA

Sequences:

>Translated_285_residues
MSAHLIDGNALAKQIRAEAAQRAARLTAAGHQPGLAVVLVGEDPASQVYVRNKVKACEDNGFYSSLNRYPADLSEADLLA
RIDELNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESIQYPLRGAR
AVVVGASNIVGKPMAMMLLQAGATVTICNSKTRDLAAHTRDADVIVAAVGRRNIITADIVKPGAVVIDVGMNRDDAGKLC
GDVDFAGVREVAGHITPVPGGVGPMTITMLLINTLEAAEREAGIA
>Mature_284_residues
SAHLIDGNALAKQIRAEAAQRAARLTAAGHQPGLAVVLVGEDPASQVYVRNKVKACEDNGFYSSLNRYPADLSEADLLAR
IDELNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESIQYPLRGARA
VVVGASNIVGKPMAMMLLQAGATVTICNSKTRDLAAHTRDADVIVAAVGRRNIITADIVKPGAVVIDVGMNRDDAGKLCG
DVDFAGVREVAGHITPVPGGVGPMTITMLLINTLEAAEREAGIA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=292, Percent_Identity=46.5753424657534, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=44.5205479452055, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=41.0344827586207, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI36796743, Length=223, Percent_Identity=27.8026905829596, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=53.9568345323741, Blast_Score=283, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=41.3559322033898, Blast_Score=204, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=46.5277777777778, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=41.5282392026578, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=295, Percent_Identity=26.7796610169492, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=48.4745762711864, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=48.4745762711864, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=46.0207612456747, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=46.0207612456747, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=46.0207612456747, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=46.0207612456747, Blast_Score=251, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_RALME (Q1LP48)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583347.1
- ProteinModelPortal:   Q1LP48
- SMR:   Q1LP48
- STRING:   Q1LP48
- GeneID:   4037994
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1192
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- PhylomeDB:   Q1LP48
- ProtClustDB:   PRK14189
- BioCyc:   RMET266264:RMET_1192-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30005; Mature: 29874

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHLIDGNALAKQIRAEAAQRAARLTAAGHQPGLAVVLVGEDPASQVYVRNKVKACEDN
CCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEEHHHHHHHCCCC
GFYSSLNRYPADLSEADLLARIDELNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVD
CCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVANAGALMTGAPLFRPCTPYGCMKMLESIQYPLRGARAVVVGASNIVGKPMAMMLLQ
CEEEECCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCHHCCCHHHHHHHH
AGATVTICNSKTRDLAAHTRDADVIVAAVGRRNIITADIVKPGAVVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHCCCCCCEEEEECCCCCEEEEECCCCCEEEEEECCCCCCHHHHC
GDVDFAGVREVAGHITPVPGGVGPMTITMLLINTLEAAEREAGIA
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAHLIDGNALAKQIRAEAAQRAARLTAAGHQPGLAVVLVGEDPASQVYVRNKVKACEDN
CCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEEHHHHHHHCCCC
GFYSSLNRYPADLSEADLLARIDELNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVD
CCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVANAGALMTGAPLFRPCTPYGCMKMLESIQYPLRGARAVVVGASNIVGKPMAMMLLQ
CEEEECCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCHHCCCHHHHHHHH
AGATVTICNSKTRDLAAHTRDADVIVAAVGRRNIITADIVKPGAVVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHCCCCCCEEEEECCCCCEEEEECCCCCEEEEEECCCCCCHHHHC
GDVDFAGVREVAGHITPVPGGVGPMTITMLLINTLEAAEREAGIA
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA