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The map label for this gene is fixJ [H]

Identifier: 94310139

GI number: 94310139

Start: 1308659

End: 1309291

Strand: Reverse

Name: fixJ [H]

Synonym: Rmet_1194

Alternate gene names: 94310139

Gene position: 1309291-1308659 (Counterclockwise)

Preceding gene: 94310140

Following gene: 94310137

Centisome position: 33.33

GC content: 63.82

Gene sequence:

>633_bases
ATGACCGCAACGCCCAGCCCCACGCCTCATCGTGGCGAGACCGTATTCATCGTCGACGACGATGAAGCCATGCGTGACTC
GCTGACCTGGCTGCTCGAGGGCAACGGCTACCAGGTGCGCAGCTTCAACAGCGCCGAGCAGTTCCTGTCCGCCTACGACG
CCAGCCAGGTTTCGTGCCTGATCCTGGACGTCCGTATGCCCGGCATGAGCGGCCCGGAGTTGCAGGAGCGCATGATCGCC
GAGAACATCGACATCCCGATCGTCTTCATCACCGGCCACGGCGACGTGCCGATGGCGGTCTCGACGATGAAGCGCGGCGC
GATCGACTTCATCGAGAAGCCGTTCGACGAATCGGAACTGCGCTCGCTGGTGGAACGGATGCTGCAGAAAGCCCGCACCG
ATCACTCGGCCGCGCGCGAGCAGCGTGCCGCCAAGGACCTGCTGAGCAAGCTGACCACGCGCGAGCAGCAGGTGCTCGAG
CGCATCGTGGCAGGCCGTCTGAACAAGCAGATCGCCGACGACCTGGGCATTTCCATCAAGACCGTGGAGGCGCACCGCGC
CAACATCATGGAAAAGCTCAACGTCAACACGGTGGCGGATCTGTTGCGCCTGGCGCTCTCGCGCAATAGCTGA

Upstream 100 bases:

>100_bases
GCGGGGCATCGGGAGGTCGTCCGGATCGTTCACCCGGGGTGTTGCGCACTGCCGCTGGCGGTTACACTTCGTTGCAAACG
ACTTTGCCCCAGGAGATTTC

Downstream 100 bases:

>100_bases
GTCCCGCCTTGCCGCTCCTTCTCCCCGTCGCGGGGGAAGGAGCCGCCTCCCTGCCCACTGCCGGCCGAACGGCGCTTGTC
CTTCCCTTGGCCGCTCGTTA

Product: LuxR family two component transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTATPSPTPHRGETVFIVDDDEAMRDSLTWLLEGNGYQVRSFNSAEQFLSAYDASQVSCLILDVRMPGMSGPELQERMIA
ENIDIPIVFITGHGDVPMAVSTMKRGAIDFIEKPFDESELRSLVERMLQKARTDHSAAREQRAAKDLLSKLTTREQQVLE
RIVAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSRNS

Sequences:

>Translated_210_residues
MTATPSPTPHRGETVFIVDDDEAMRDSLTWLLEGNGYQVRSFNSAEQFLSAYDASQVSCLILDVRMPGMSGPELQERMIA
ENIDIPIVFITGHGDVPMAVSTMKRGAIDFIEKPFDESELRSLVERMLQKARTDHSAAREQRAAKDLLSKLTTREQQVLE
RIVAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSRNS
>Mature_209_residues
TATPSPTPHRGETVFIVDDDEAMRDSLTWLLEGNGYQVRSFNSAEQFLSAYDASQVSCLILDVRMPGMSGPELQERMIAE
NIDIPIVFITGHGDVPMAVSTMKRGAIDFIEKPFDESELRSLVERMLQKARTDHSAAREQRAAKDLLSKLTTREQQVLER
IVAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSRNS

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=114, Percent_Identity=35.9649122807018, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790102, Length=194, Percent_Identity=30.9278350515464, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788222, Length=202, Percent_Identity=26.2376237623762, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788550, Length=115, Percent_Identity=29.5652173913043, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790437, Length=119, Percent_Identity=33.6134453781513, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786747, Length=201, Percent_Identity=24.3781094527363, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1789809, Length=145, Percent_Identity=29.6551724137931, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=120, Percent_Identity=28.3333333333333, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790863, Length=141, Percent_Identity=29.7872340425532, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788905, Length=122, Percent_Identity=27.8688524590164, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23438; Mature: 23307

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPSPTPHRGETVFIVDDDEAMRDSLTWLLEGNGYQVRSFNSAEQFLSAYDASQVSCL
CCCCCCCCCCCCCEEEEECCCHHHHHHHEEEECCCCEEEECCCHHHHHHHHHCCCCEEEE
ILDVRMPGMSGPELQERMIAENIDIPIVFITGHGDVPMAVSTMKRGAIDFIEKPFDESEL
EEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHH
RSLVERMLQKARTDHSAAREQRAAKDLLSKLTTREQQVLERIVAGRLNKQIADDLGISIK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
TVEAHRANIMEKLNVNTVADLLRLALSRNS
EHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TATPSPTPHRGETVFIVDDDEAMRDSLTWLLEGNGYQVRSFNSAEQFLSAYDASQVSCL
CCCCCCCCCCCCEEEEECCCHHHHHHHEEEECCCCEEEECCCHHHHHHHHHCCCCEEEE
ILDVRMPGMSGPELQERMIAENIDIPIVFITGHGDVPMAVSTMKRGAIDFIEKPFDESEL
EEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHH
RSLVERMLQKARTDHSAAREQRAAKDLLSKLTTREQQVLERIVAGRLNKQIADDLGISIK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
TVEAHRANIMEKLNVNTVADLLRLALSRNS
EHHHHHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]