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The map label for this gene is ybdL [H]

Identifier: 94309921

GI number: 94309921

Start: 1062002

End: 1063171

Strand: Reverse

Name: ybdL [H]

Synonym: Rmet_0976

Alternate gene names: 94309921

Gene position: 1063171-1062002 (Counterclockwise)

Preceding gene: 94309922

Following gene: 94309919

Centisome position: 27.07

GC content: 64.1

Gene sequence:

>1170_bases
ATGGCTGAATCCAACCTCGCCCCGCTGAATCCGCCCCCGTCGCGCCTGCCCAACGTGGGCACCACGATCTTTACCGTGAT
GTCAGCGCTGGCGGCCGAAAAGAACGCCGTCAATCTCGGCCAGGGCTTCCCGGATTTCGACTGCGACCCGCGCATCGTCG
ACGCCGTGACGCACGCAATGCGCACCGGTCACAACCAGTACCCGCCGATGGCCGGTGTGCCACAACTGCGCCAGGCCATT
GCCGCCAAGATCGCGAAGCTGTACGAACACACCTACGACTGGAATACCGAGATCACGGTGACCGCGGGCGCCACGCAGGG
CCTGTTGACCGCCATCCTCTGCGCCGTGCATCCGGGTGACGAAGTGATCGTGCTGGAACCGTGCTACGACAGCTATCTGC
CTGCAATCGAACTGGCCGGCGCGACGGCCGTGCCCGTGACGCTGGAAGCGCCGCTGTTCCGCATCCCGTTCGACAAGCTG
GCCGCCGCGATCACGCCGCGCACGCGCATGATCATGATCAACACCCCGCACAACCCGACGGGCACGATCTGGCGCGAAGA
GGACATGCAGCAACTGGCCGACCTGCTGGCCGGCACCGACATCCTGCTGCTGTCCGACGAGGTCTACGAGCACATGGTCT
ACGATGGCCAGCAGCACGAGTCGGTGGCACGCCATCCCGAACTGGCGCGACGCAGCTTTGTCGTGTCGAGCTTCGGCAAG
ACCTATCACGTGACGGGCTGGAAGGTCGGCTTCGTGGCCGCCCCGGCCGCGCTGTCTAACGAATTCCGCAAGGTCCACCA
GTTCAACGTGTTCACGGTGAACACGCCGGTGCAGTACGGGCTGGCGGATTACATGGCCGACCCGGCCCCGTACCTGAACC
TCTCCGCGTTCTACCAAGCCAAGCGCGACTACTTCCGTGCGGGCCTGGCCGGCACGCGCTTCAAGCTTCTGCCATCGGAC
GGCACCTACTTCCAGTGCGTCGACTACTCGGCGATCTCCGATATGAGCGAGGCCGATTTCTCGATGTGGCTCACGCGCGA
AATCGGCGTTGCCGCGATTCCGGTCTCGGCGTTCTACTCCACGCCGCGTGAATCGGGTGTCGTGCGCTTCTGTTTCGCGA
AGAAGGAAGAGACGCTGAAGCTGGCGCTGGATCGTCTGGCAAAGCTCTGA

Upstream 100 bases:

>100_bases
GGCATTCGGCGGCTGGCTGGCGGCATGGGTGCCGCCCGATGATCGCGGCTAGAATGTCGCTCTGGCTGATTGCCCCGCTT
CTACTCTTTCAAGCTCCGTC

Downstream 100 bases:

>100_bases
TCCGAATCGGAGGTTTGCGCGCCCCACTCCCACAACGTGGAAGTGGGGTGATCCCCCTTCTTACTTGTTCCTGGCGGCCA
TCTGTTCCAGATTCGCCTTG

Product: methionine aminotransferase

Products: oxaloacetate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MAESNLAPLNPPPSRLPNVGTTIFTVMSALAAEKNAVNLGQGFPDFDCDPRIVDAVTHAMRTGHNQYPPMAGVPQLRQAI
AAKIAKLYEHTYDWNTEITVTAGATQGLLTAILCAVHPGDEVIVLEPCYDSYLPAIELAGATAVPVTLEAPLFRIPFDKL
AAAITPRTRMIMINTPHNPTGTIWREEDMQQLADLLAGTDILLLSDEVYEHMVYDGQQHESVARHPELARRSFVVSSFGK
TYHVTGWKVGFVAAPAALSNEFRKVHQFNVFTVNTPVQYGLADYMADPAPYLNLSAFYQAKRDYFRAGLAGTRFKLLPSD
GTYFQCVDYSAISDMSEADFSMWLTREIGVAAIPVSAFYSTPRESGVVRFCFAKKEETLKLALDRLAKL

Sequences:

>Translated_389_residues
MAESNLAPLNPPPSRLPNVGTTIFTVMSALAAEKNAVNLGQGFPDFDCDPRIVDAVTHAMRTGHNQYPPMAGVPQLRQAI
AAKIAKLYEHTYDWNTEITVTAGATQGLLTAILCAVHPGDEVIVLEPCYDSYLPAIELAGATAVPVTLEAPLFRIPFDKL
AAAITPRTRMIMINTPHNPTGTIWREEDMQQLADLLAGTDILLLSDEVYEHMVYDGQQHESVARHPELARRSFVVSSFGK
TYHVTGWKVGFVAAPAALSNEFRKVHQFNVFTVNTPVQYGLADYMADPAPYLNLSAFYQAKRDYFRAGLAGTRFKLLPSD
GTYFQCVDYSAISDMSEADFSMWLTREIGVAAIPVSAFYSTPRESGVVRFCFAKKEETLKLALDRLAKL
>Mature_388_residues
AESNLAPLNPPPSRLPNVGTTIFTVMSALAAEKNAVNLGQGFPDFDCDPRIVDAVTHAMRTGHNQYPPMAGVPQLRQAIA
AKIAKLYEHTYDWNTEITVTAGATQGLLTAILCAVHPGDEVIVLEPCYDSYLPAIELAGATAVPVTLEAPLFRIPFDKLA
AAITPRTRMIMINTPHNPTGTIWREEDMQQLADLLAGTDILLLSDEVYEHMVYDGQQHESVARHPELARRSFVVSSFGKT
YHVTGWKVGFVAAPAALSNEFRKVHQFNVFTVNTPVQYGLADYMADPAPYLNLSAFYQAKRDYFRAGLAGTRFKLLPSDG
TYFQCVDYSAISDMSEADFSMWLTREIGVAAIPVSAFYSTPRESGVVRFCFAKKEETLKLALDRLAKL

Specific function: Shows aminotransferase activity with methionine and histidine as substrates, and to a lesser extent also with phenylalanine [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI56713254, Length=399, Percent_Identity=35.5889724310777, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI56713256, Length=399, Percent_Identity=35.5889724310777, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI95147551, Length=409, Percent_Identity=33.4963325183374, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI169881279, Length=409, Percent_Identity=33.4963325183374, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI169881281, Length=407, Percent_Identity=30.2211302211302, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI4507369, Length=340, Percent_Identity=24.4117647058824, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786816, Length=383, Percent_Identity=50.6527415143603, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1788722, Length=364, Percent_Identity=25.2747252747253, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788627, Length=158, Percent_Identity=29.746835443038, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787710, Length=396, Percent_Identity=26.7676767676768, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787909, Length=112, Percent_Identity=33.0357142857143, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994476, Length=421, Percent_Identity=35.3919239904988, Blast_Score=234, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI71994472, Length=421, Percent_Identity=35.3919239904988, Blast_Score=234, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17567369, Length=410, Percent_Identity=32.9268292682927, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6322401, Length=402, Percent_Identity=30.8457711442786, Blast_Score=182, Evalue=9e-47,
Organism=Drosophila melanogaster, GI28573069, Length=410, Percent_Identity=32.9268292682927, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24646114, Length=410, Percent_Identity=32.9268292682927, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI28573067, Length=410, Percent_Identity=32.9268292682927, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI28573065, Length=410, Percent_Identity=32.9268292682927, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI18859735, Length=207, Percent_Identity=26.5700483091787, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.1

Molecular weight: Translated: 42872; Mature: 42741

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESNLAPLNPPPSRLPNVGTTIFTVMSALAAEKNAVNLGQGFPDFDCDPRIVDAVTHAM
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RTGHNQYPPMAGVPQLRQAIAAKIAKLYEHTYDWNTEITVTAGATQGLLTAILCAVHPGD
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCC
EVIVLEPCYDSYLPAIELAGATAVPVTLEAPLFRIPFDKLAAAITPRTRMIMINTPHNPT
CEEEEECCCCCCCCHHHCCCCEECCEEECCCEEECCHHHHHHHCCCCEEEEEEECCCCCC
GTIWREEDMQQLADLLAGTDILLLSDEVYEHMVYDGQQHESVARHPELARRSFVVSSFGK
CCEECHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHCCC
TYHVTGWKVGFVAAPAALSNEFRKVHQFNVFTVNTPVQYGLADYMADPAPYLNLSAFYQA
EEEECCEEEEEEECCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHH
KRDYFRAGLAGTRFKLLPSDGTYFQCVDYSAISDMSEADFSMWLTREIGVAAIPVSAFYS
HHHHHHHCCCCCEEEEECCCCCEEEEECCHHHCCCCHHHHHEEEECCCCEEEEEHHHHHC
TPRESGVVRFCFAKKEETLKLALDRLAKL
CCCCCCEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AESNLAPLNPPPSRLPNVGTTIFTVMSALAAEKNAVNLGQGFPDFDCDPRIVDAVTHAM
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RTGHNQYPPMAGVPQLRQAIAAKIAKLYEHTYDWNTEITVTAGATQGLLTAILCAVHPGD
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCC
EVIVLEPCYDSYLPAIELAGATAVPVTLEAPLFRIPFDKLAAAITPRTRMIMINTPHNPT
CEEEEECCCCCCCCHHHCCCCEECCEEECCCEEECCHHHHHHHCCCCEEEEEEECCCCCC
GTIWREEDMQQLADLLAGTDILLLSDEVYEHMVYDGQQHESVARHPELARRSFVVSSFGK
CCEECHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHCCC
TYHVTGWKVGFVAAPAALSNEFRKVHQFNVFTVNTPVQYGLADYMADPAPYLNLSAFYQA
EEEECCEEEEEEECCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHH
KRDYFRAGLAGTRFKLLPSDGTYFQCVDYSAISDMSEADFSMWLTREIGVAAIPVSAFYS
HHHHHHHCCCCCEEEEECCCCCEEEEECCHHHCCCCHHHHHEEEECCCCEEEEEHHHHHC
TPRESGVVRFCFAKKEETLKLALDRLAKL
CCCCCCEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate; 2-oxoglutarate

Specific reaction: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]