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The map label for this gene is gstI [H]

Identifier: 94309912

GI number: 94309912

Start: 1050834

End: 1051526

Strand: Reverse

Name: gstI [H]

Synonym: Rmet_0967

Alternate gene names: 94309912

Gene position: 1051526-1050834 (Counterclockwise)

Preceding gene: 94309913

Following gene: 94309910

Centisome position: 26.77

GC content: 63.06

Gene sequence:

>693_bases
ATGATCGACGCCTACACCTGGCCCACACCGAACGGCCATAAAGTCCACATCATGCTTGAGGAGTGCGGCCTCGAGTACAG
GGTCCATCCGATCAACATCGGCGCCGGCGACCAGTTCGGCGAGGACTTTCTCAAGATCAGCCCGAACAACAAGATCCCGG
CGATCGTCGACACCGACGGCCCGGACGGCCAGCCGATCTCGCTGTTCGAATCGGGTGCGATCCTGCTGTACCTGGCGGGC
AAGACCGGCAAGTTCCTGCCCGAGGACGTGCGCGGCAAGTACGAGGCGCTGCAGTGGCTGATGTTCCAGATGGGCGGTGT
CGGCCCGATGCTGGGTCAGGCGCACCACTTCCGCATCTACGCGCCGGAGAAGATCGAGTACGCCGTCAATCGCTATACCA
ACGAGGCCAAGCGCCTCTACGGCGTGATCGACAAGCAGTTGTCGACGCACAAGTATCTGGCAGGCGACACCTACACCGTG
GCCGATATCGCAACGTTCCCGTGGCTGCGCAGCTGGCAGAACCAGGGCGTGGAATTGGACGACTATCCGCACCTGAAGCG
CTGGTTCAACGACATCGCGGAACGTCCAGCGGTCAAGCGCGGCGTCGAGGTCCTGGCCAGCGCACGCAAGCCGCTGCAGG
GCGACAAGGAGCGCGAGGTCCTGTTCGGCGCCACGCAATACCAGCGTCGCTAA

Upstream 100 bases:

>100_bases
ACACCGAAGCACCATCCCACGAACAGATCATCCATGCCCAACAGGGTTATTTCTTTCGTGCTCTAATGCCCCGGCGAATC
TTCCATGGAGACAGTTGCAG

Downstream 100 bases:

>100_bases
TGCCGGCGTCAGGCGGCCTGTCGTTCGCCCTGCGGGGCGTATGCGTCTGACGGTACCGGTACCGGTGCCGGTGCCGGTGC
CGGCTGCATGCAGCAGAGCA

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MIDAYTWPTPNGHKVHIMLEECGLEYRVHPINIGAGDQFGEDFLKISPNNKIPAIVDTDGPDGQPISLFESGAILLYLAG
KTGKFLPEDVRGKYEALQWLMFQMGGVGPMLGQAHHFRIYAPEKIEYAVNRYTNEAKRLYGVIDKQLSTHKYLAGDTYTV
ADIATFPWLRSWQNQGVELDDYPHLKRWFNDIAERPAVKRGVEVLASARKPLQGDKEREVLFGATQYQRR

Sequences:

>Translated_230_residues
MIDAYTWPTPNGHKVHIMLEECGLEYRVHPINIGAGDQFGEDFLKISPNNKIPAIVDTDGPDGQPISLFESGAILLYLAG
KTGKFLPEDVRGKYEALQWLMFQMGGVGPMLGQAHHFRIYAPEKIEYAVNRYTNEAKRLYGVIDKQLSTHKYLAGDTYTV
ADIATFPWLRSWQNQGVELDDYPHLKRWFNDIAERPAVKRGVEVLASARKPLQGDKEREVLFGATQYQRR
>Mature_230_residues
MIDAYTWPTPNGHKVHIMLEECGLEYRVHPINIGAGDQFGEDFLKISPNNKIPAIVDTDGPDGQPISLFESGAILLYLAG
KTGKFLPEDVRGKYEALQWLMFQMGGVGPMLGQAHHFRIYAPEKIEYAVNRYTNEAKRLYGVIDKQLSTHKYLAGDTYTV
ADIATFPWLRSWQNQGVELDDYPHLKRWFNDIAERPAVKRGVEVLASARKPLQGDKEREVLFGATQYQRR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=203, Percent_Identity=52.7093596059113, Blast_Score=223, Evalue=6e-60,
Organism=Escherichia coli, GI87082195, Length=215, Percent_Identity=49.7674418604651, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081789, Length=187, Percent_Identity=30.4812834224599, Blast_Score=83, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324100, Length=232, Percent_Identity=32.3275862068966, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28571670, Length=174, Percent_Identity=29.3103448275862, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17933730, Length=228, Percent_Identity=27.1929824561404, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17864592, Length=199, Percent_Identity=29.6482412060301, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17864598, Length=205, Percent_Identity=27.8048780487805, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI85725204, Length=203, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17737923, Length=203, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17864594, Length=192, Percent_Identity=28.125, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI45549270, Length=194, Percent_Identity=26.8041237113402, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26153; Mature: 26153

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDAYTWPTPNGHKVHIMLEECGLEYRVHPINIGAGDQFGEDFLKISPNNKIPAIVDTDG
CCCCCCCCCCCCCEEEEEEHHCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCEEEECCC
PDGQPISLFESGAILLYLAGKTGKFLPEDVRGKYEALQWLMFQMGGVGPMLGQAHHFRIY
CCCCEEEEECCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHCCCCEEEEE
APEKIEYAVNRYTNEAKRLYGVIDKQLSTHKYLAGDTYTVADIATFPWLRSWQNQGVELD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEHHHHHHHHHHHHHHHCCCCCC
DYPHLKRWFNDIAERPAVKRGVEVLASARKPLQGDKEREVLFGATQYQRR
CCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHCCC
>Mature Secondary Structure
MIDAYTWPTPNGHKVHIMLEECGLEYRVHPINIGAGDQFGEDFLKISPNNKIPAIVDTDG
CCCCCCCCCCCCCEEEEEEHHCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCEEEECCC
PDGQPISLFESGAILLYLAGKTGKFLPEDVRGKYEALQWLMFQMGGVGPMLGQAHHFRIY
CCCCEEEEECCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHCCCCEEEEE
APEKIEYAVNRYTNEAKRLYGVIDKQLSTHKYLAGDTYTVADIATFPWLRSWQNQGVELD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEHHHHHHHHHHHHHHHCCCCCC
DYPHLKRWFNDIAERPAVKRGVEVLASARKPLQGDKEREVLFGATQYQRR
CCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]