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The map label for this gene is braD [H]

Identifier: 94309898

GI number: 94309898

Start: 1038981

End: 1040618

Strand: Direct

Name: braD [H]

Synonym: Rmet_0953

Alternate gene names: 94309898

Gene position: 1038981-1040618 (Clockwise)

Preceding gene: 94309897

Following gene: 94309899

Centisome position: 26.45

GC content: 66.42

Gene sequence:

>1638_bases
ATGCACCGACCCAATATCCGTACATGGCTGCGTGTCATGCTCGCCGCGCTGCTGCTGGCCGCGGCACCGTTGTGGGCCGC
CACGCCGCTGACGCAGGCCGACCTCAAGCCATTGGCCGAGGATGACTACGATGCCAAGACCGCGGCGCTGAACAAGCTCA
CTCAGGCGCCGGCGGAGCAGGCCGGCCCGATCCTGAAGGCCTTGCAGGATGACGCGCTGCAGTACGCGCCCTCAGTCGGC
ATGGTGCGCCAGGATGGCGACAAGACCGTCGACGCGGTCACCGGCAAGCCGGTCATCGTGAAGGCGGACGAGCTCGAATC
GCTGACGCTGAATAACAGCCTGCGCGCCATCGTCGATAGTGCCGCGAGCAGCCTGGAGCTGCAGTCACCGGACATCGCGG
TGCGCACGCAGGCCATCAGTACGCTGTTCGATCAACCAGAGAACGCTTCGCGTGCGGCGGTGGAGGCCGCGCGCAAGGCC
GAGGCCGATGCCGGACTGCGGGCGAGGCTCGACGTGATCTGGGCCAACACGGTTCTCGCGCAAGGTGCCGAAGCGGGCCG
GGACGCGCGTATCGAGGCGATTCGCATCCTCGGCCACGACAGCAATCCGCAATCGCGCCAGAAGCTGATGCCGCTGGTCG
AGCGCGACGCGGCCGGCAAGTACAAAGAGGCCGACGAAGGCGTGCGCGTGGCCGCGCAAGTGGCCATTGACCAGTTGCGG
ACCGAACAGCGCCGCGCCGAACTGCTCGGCAACCTGTTCGCGGGTCTGAGCCTTGGCAGCGTGCTGCTGCTGGCGGCGCT
GGGTCTGGCGATCACGTATGGGCTGATCGGCGTCATCAACATGGCGCACGGCGAGTTTCTGATGATCGGCGCCTATGCCA
CCTACGTGGTGCAGACGCTGTTTCGTGCCTGCGCGCCCAATGCGTTCGACTGGTACCTCGTGGCGGCCGTGCCTGCGTCC
TTCCTGGCTGCCGGAGCCGTCGGTTTCGCGCTCGAGCGAATCGTGCTGCGCCATCTCTATGGCCGCCCGCTGGAGACGCT
GCTGGCCACGTTCGGTGTCAGCCTGCTGATGATGCAGGCCGTGCGTACGATCTTCGGCGCGCAGAACGTGGAAGTGGCCA
ATCCCGCGTGGATGAGCGGCGGCATCGAGGCACTCGCCGGGCTCGTGATTCCGTACAACCGCATCATCATCATCCTGTTC
GCGCTGGGCGTGGTGACGGTGGCATGGGCCGTACTGAATCGCACGCGCCTGGGTCTGTTCGTGCGCGCCACGACGCAGAA
CCGCACGATGGCGGCCTGCGTGGGCGTGCGGACGTGGAAGGTGGACAGCTACGCGTTCGCATTTGGCGCCGGCATCGCGG
GGCTCGGCGGTTGCGCGCTCTCGCAGATCGGTAACGTGGGCCCGGACCTGGGGCAGGCCTACATCATCGACTCGTTCATG
GTGGTGGTGCTGGGTGGCGTCGGGCAGCTGGCGGGCACGATCGTGGGCGCGTTCGGCCTCGGCATCATCAACAAATTCAT
CGAGCCGTTCTATGGCGCGGTGCTGGCCAAGATCTTCGTCCTCGTGCTGATCGTGCTGTTCATCCAGAAGCGGCCACAAG
GACTTTTCGCGCTCAAGGGGCGCAGCGCGGAGGCCTGA

Upstream 100 bases:

>100_bases
CGGTCTGTTGCTCCCACTCCCGTGCTCGGGAGTGGGAGCAAACCACAGGTGAATGCCAACAACCCCAAGATGCGATCCAT
CGCGCGAACCAGGCTCCTGA

Downstream 100 bases:

>100_bases
CATGAGCAGCTTTTCCTCGAAATCCGGCAAGTCATTCACGCTATCCGTGCCGGAACGCCAGCCGCTGTTCTCCACGCGCA
TGTGGATGGCGATGGCGGTA

Product: putative branched-chain amino acid permease protein (ABC superfamily)

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MHRPNIRTWLRVMLAALLLAAAPLWAATPLTQADLKPLAEDDYDAKTAALNKLTQAPAEQAGPILKALQDDALQYAPSVG
MVRQDGDKTVDAVTGKPVIVKADELESLTLNNSLRAIVDSAASSLELQSPDIAVRTQAISTLFDQPENASRAAVEAARKA
EADAGLRARLDVIWANTVLAQGAEAGRDARIEAIRILGHDSNPQSRQKLMPLVERDAAGKYKEADEGVRVAAQVAIDQLR
TEQRRAELLGNLFAGLSLGSVLLLAALGLAITYGLIGVINMAHGEFLMIGAYATYVVQTLFRACAPNAFDWYLVAAVPAS
FLAAGAVGFALERIVLRHLYGRPLETLLATFGVSLLMMQAVRTIFGAQNVEVANPAWMSGGIEALAGLVIPYNRIIIILF
ALGVVTVAWAVLNRTRLGLFVRATTQNRTMAACVGVRTWKVDSYAFAFGAGIAGLGGCALSQIGNVGPDLGQAYIIDSFM
VVVLGGVGQLAGTIVGAFGLGIINKFIEPFYGAVLAKIFVLVLIVLFIQKRPQGLFALKGRSAEA

Sequences:

>Translated_545_residues
MHRPNIRTWLRVMLAALLLAAAPLWAATPLTQADLKPLAEDDYDAKTAALNKLTQAPAEQAGPILKALQDDALQYAPSVG
MVRQDGDKTVDAVTGKPVIVKADELESLTLNNSLRAIVDSAASSLELQSPDIAVRTQAISTLFDQPENASRAAVEAARKA
EADAGLRARLDVIWANTVLAQGAEAGRDARIEAIRILGHDSNPQSRQKLMPLVERDAAGKYKEADEGVRVAAQVAIDQLR
TEQRRAELLGNLFAGLSLGSVLLLAALGLAITYGLIGVINMAHGEFLMIGAYATYVVQTLFRACAPNAFDWYLVAAVPAS
FLAAGAVGFALERIVLRHLYGRPLETLLATFGVSLLMMQAVRTIFGAQNVEVANPAWMSGGIEALAGLVIPYNRIIIILF
ALGVVTVAWAVLNRTRLGLFVRATTQNRTMAACVGVRTWKVDSYAFAFGAGIAGLGGCALSQIGNVGPDLGQAYIIDSFM
VVVLGGVGQLAGTIVGAFGLGIINKFIEPFYGAVLAKIFVLVLIVLFIQKRPQGLFALKGRSAEA
>Mature_545_residues
MHRPNIRTWLRVMLAALLLAAAPLWAATPLTQADLKPLAEDDYDAKTAALNKLTQAPAEQAGPILKALQDDALQYAPSVG
MVRQDGDKTVDAVTGKPVIVKADELESLTLNNSLRAIVDSAASSLELQSPDIAVRTQAISTLFDQPENASRAAVEAARKA
EADAGLRARLDVIWANTVLAQGAEAGRDARIEAIRILGHDSNPQSRQKLMPLVERDAAGKYKEADEGVRVAAQVAIDQLR
TEQRRAELLGNLFAGLSLGSVLLLAALGLAITYGLIGVINMAHGEFLMIGAYATYVVQTLFRACAPNAFDWYLVAAVPAS
FLAAGAVGFALERIVLRHLYGRPLETLLATFGVSLLMMQAVRTIFGAQNVEVANPAWMSGGIEALAGLVIPYNRIIIILF
ALGVVTVAWAVLNRTRLGLFVRATTQNRTMAACVGVRTWKVDSYAFAFGAGIAGLGGCALSQIGNVGPDLGQAYIIDSFM
VVVLGGVGQLAGTIVGAFGLGIINKFIEPFYGAVLAKIFVLVLIVLFIQKRPQGLFALKGRSAEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=300, Percent_Identity=27.3333333333333, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 57909; Mature: 57909

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRPNIRTWLRVMLAALLLAAAPLWAATPLTQADLKPLAEDDYDAKTAALNKLTQAPAEQ
CCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCHHH
AGPILKALQDDALQYAPSVGMVRQDGDKTVDAVTGKPVIVKADELESLTLNNSLRAIVDS
HHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHCCCCCEEEECCCCCHHHHCCHHHHHHHH
AASSLELQSPDIAVRTQAISTLFDQPENASRAAVEAARKAEADAGLRARLDVIWANTVLA
HHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QGAEAGRDARIEAIRILGHDSNPQSRQKLMPLVERDAAGKYKEADEGVRVAAQVAIDQLR
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
TEQRRAELLGNLFAGLSLGSVLLLAALGLAITYGLIGVINMAHGEFLMIGAYATYVVQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
FRACAPNAFDWYLVAAVPASFLAAGAVGFALERIVLRHLYGRPLETLLATFGVSLLMMQA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VRTIFGAQNVEVANPAWMSGGIEALAGLVIPYNRIIIILFALGVVTVAWAVLNRTRLGLF
HHHHHCCCCCEECCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VRATTQNRTMAACVGVRTWKVDSYAFAFGAGIAGLGGCALSQIGNVGPDLGQAYIIDSFM
EEEECCCCCHHHHHCCEEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VVVLGGVGQLAGTIVGAFGLGIINKFIEPFYGAVLAKIFVLVLIVLFIQKRPQGLFALKG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
RSAEA
CCCCC
>Mature Secondary Structure
MHRPNIRTWLRVMLAALLLAAAPLWAATPLTQADLKPLAEDDYDAKTAALNKLTQAPAEQ
CCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCHHH
AGPILKALQDDALQYAPSVGMVRQDGDKTVDAVTGKPVIVKADELESLTLNNSLRAIVDS
HHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHCCCCCEEEECCCCCHHHHCCHHHHHHHH
AASSLELQSPDIAVRTQAISTLFDQPENASRAAVEAARKAEADAGLRARLDVIWANTVLA
HHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QGAEAGRDARIEAIRILGHDSNPQSRQKLMPLVERDAAGKYKEADEGVRVAAQVAIDQLR
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
TEQRRAELLGNLFAGLSLGSVLLLAALGLAITYGLIGVINMAHGEFLMIGAYATYVVQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
FRACAPNAFDWYLVAAVPASFLAAGAVGFALERIVLRHLYGRPLETLLATFGVSLLMMQA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VRTIFGAQNVEVANPAWMSGGIEALAGLVIPYNRIIIILFALGVVTVAWAVLNRTRLGLF
HHHHHCCCCCEECCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VRATTQNRTMAACVGVRTWKVDSYAFAFGAGIAGLGGCALSQIGNVGPDLGQAYIIDSFM
EEEECCCCCHHHHHCCEEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VVVLGGVGQLAGTIVGAFGLGIINKFIEPFYGAVLAKIFVLVLIVLFIQKRPQGLFALKG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
RSAEA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]