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The map label for this gene is livM [H]

Identifier: 94309899

GI number: 94309899

Start: 1040620

End: 1041822

Strand: Direct

Name: livM [H]

Synonym: Rmet_0954

Alternate gene names: 94309899

Gene position: 1040620-1041822 (Clockwise)

Preceding gene: 94309898

Following gene: 94309900

Centisome position: 26.49

GC content: 63.18

Gene sequence:

>1203_bases
ATGAGCAGCTTTTCCTCGAAATCCGGCAAGTCATTCACGCTATCCGTGCCGGAACGCCAGCCGCTGTTCTCCACGCGCAT
GTGGATGGCGATGGCGGTACTGACCGTGGTCGTGGCGATCGGCGTGCCAGTCTGCGCGCTGGTCGTGCCGGAAGGCCACG
CGCTGCATCTGTCGGCCTATGCCTTGACGTTGATCGGCAAGATCATGTGCTTCGCGCTGGCGGCCATTGCGCTCGATCTG
GTGTGGGGCTATTGCGGCATCCTGAGCCTCGGCCATGGTCTGTTCTTCGCGCTGGGCGGGTATGCGATGGGCATGTACCT
GATGCGGTCGATCGGTCGCGATGGTGTCTACAAGAGCGATCTGCCCGACTTCATGGTGTTCCTGGACTGGAAGGAGCTGC
CGTGGTTCTGGCATGGCACCGAGCACTTTCCCTACGCATTGCTGCTGGTGGTACTGGTGCCGGGCGTGCTGGCGTGGCTG
TTTGGCTTCTTCGCGTTCCGGTCCCGCATCAAGGGTGTGTACCTGTCGATCATCACGCAGGCTATGACGTACGCCGCGAT
GCTGCTGTTCTTCCGCAATGAAACGGGCTTCGGCGGCAATAACGGCTTTACCGACTTCAAGCGCATCGCCGGTTTTGCCG
TGGCTGCGCCGCAGACGCGTGCCGCGCTGTTCGTGCTGACGTTCCTTGCACTGCTGGCCGGATTCGTGGCCTGCCGCTAC
ATCGTCACGTCCAAGCTCGGCCGGGTGGTGACCGCCGTGCGCGACGCGGAGATGCGCGTGATGTTTTCGGGCTACAACCC
GCTCGGGTACAAGCTGTTCGTGTGGACGTTCTCGGCTGTGCTTTGCGGCATTGCCGGAGCGCTGTACGTGCCGCAGGTCG
GCATCATCAACCCGAGCGAGATGTCGCCCGCGAACTCGATCGAAATGGCGGTGTGGGTCGCCGTCGGCGGGCGCGGCACG
CTGATCGGGCCGGTAGTGGGGGCATTCCTGGTCAACGGCGCGAAGACGATGTTCACGGCCTACTTCGCGGAGTACTGGCT
ATTCCTGCTCGGCGCGATGTTCGTGCTGGTGACGCTGTACCTGCCAGATGGCGTGGTGGGCCTCTGGCGACGCCTGCGTG
ATCGGCGCACTTCGAAGGTAGAAACCGGAGCCGCCGATGAAGCGGAAGCCGTGGTGGCGTCGCAGCAAGGGAGCAAGGCA
TGA

Upstream 100 bases:

>100_bases
GTGCTGGCCAAGATCTTCGTCCTCGTGCTGATCGTGCTGTTCATCCAGAAGCGGCCACAAGGACTTTTCGCGCTCAAGGG
GCGCAGCGCGGAGGCCTGAC

Downstream 100 bases:

>100_bases
ATTCGGTACTGGGTGAAGGCAAGGCGGAAAGCGGGGATGCCACCGGGCTGGGCCGGATCGTCGAACCCGGCGTCATCGAC
ACCACGCACGGTCCGATCCT

Product: putative branched-chain amino acid ABC transporter, permease protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSSFSSKSGKSFTLSVPERQPLFSTRMWMAMAVLTVVVAIGVPVCALVVPEGHALHLSAYALTLIGKIMCFALAAIALDL
VWGYCGILSLGHGLFFALGGYAMGMYLMRSIGRDGVYKSDLPDFMVFLDWKELPWFWHGTEHFPYALLLVVLVPGVLAWL
FGFFAFRSRIKGVYLSIITQAMTYAAMLLFFRNETGFGGNNGFTDFKRIAGFAVAAPQTRAALFVLTFLALLAGFVACRY
IVTSKLGRVVTAVRDAEMRVMFSGYNPLGYKLFVWTFSAVLCGIAGALYVPQVGIINPSEMSPANSIEMAVWVAVGGRGT
LIGPVVGAFLVNGAKTMFTAYFAEYWLFLLGAMFVLVTLYLPDGVVGLWRRLRDRRTSKVETGAADEAEAVVASQQGSKA

Sequences:

>Translated_400_residues
MSSFSSKSGKSFTLSVPERQPLFSTRMWMAMAVLTVVVAIGVPVCALVVPEGHALHLSAYALTLIGKIMCFALAAIALDL
VWGYCGILSLGHGLFFALGGYAMGMYLMRSIGRDGVYKSDLPDFMVFLDWKELPWFWHGTEHFPYALLLVVLVPGVLAWL
FGFFAFRSRIKGVYLSIITQAMTYAAMLLFFRNETGFGGNNGFTDFKRIAGFAVAAPQTRAALFVLTFLALLAGFVACRY
IVTSKLGRVVTAVRDAEMRVMFSGYNPLGYKLFVWTFSAVLCGIAGALYVPQVGIINPSEMSPANSIEMAVWVAVGGRGT
LIGPVVGAFLVNGAKTMFTAYFAEYWLFLLGAMFVLVTLYLPDGVVGLWRRLRDRRTSKVETGAADEAEAVVASQQGSKA
>Mature_399_residues
SSFSSKSGKSFTLSVPERQPLFSTRMWMAMAVLTVVVAIGVPVCALVVPEGHALHLSAYALTLIGKIMCFALAAIALDLV
WGYCGILSLGHGLFFALGGYAMGMYLMRSIGRDGVYKSDLPDFMVFLDWKELPWFWHGTEHFPYALLLVVLVPGVLAWLF
GFFAFRSRIKGVYLSIITQAMTYAAMLLFFRNETGFGGNNGFTDFKRIAGFAVAAPQTRAALFVLTFLALLAGFVACRYI
VTSKLGRVVTAVRDAEMRVMFSGYNPLGYKLFVWTFSAVLCGIAGALYVPQVGIINPSEMSPANSIEMAVWVAVGGRGTL
IGPVVGAFLVNGAKTMFTAYFAEYWLFLLGAMFVLVTLYLPDGVVGLWRRLRDRRTSKVETGAADEAEAVVASQQGSKA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=348, Percent_Identity=23.2758620689655, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 43606; Mature: 43475

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFSSKSGKSFTLSVPERQPLFSTRMWMAMAVLTVVVAIGVPVCALVVPEGHALHLSAY
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEHHHH
ALTLIGKIMCFALAAIALDLVWGYCGILSLGHGLFFALGGYAMGMYLMRSIGRDGVYKSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LPDFMVFLDWKELPWFWHGTEHFPYALLLVVLVPGVLAWLFGFFAFRSRIKGVYLSIITQ
CCCCEEEEEHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMTYAAMLLFFRNETGFGGNNGFTDFKRIAGFAVAAPQTRAALFVLTFLALLAGFVACRY
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IVTSKLGRVVTAVRDAEMRVMFSGYNPLGYKLFVWTFSAVLCGIAGALYVPQVGIINPSE
HHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
MSPANSIEMAVWVAVGGRGTLIGPVVGAFLVNGAKTMFTAYFAEYWLFLLGAMFVLVTLY
CCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPDGVVGLWRRLRDRRTSKVETGAADEAEAVVASQQGSKA
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSFSSKSGKSFTLSVPERQPLFSTRMWMAMAVLTVVVAIGVPVCALVVPEGHALHLSAY
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEHHHH
ALTLIGKIMCFALAAIALDLVWGYCGILSLGHGLFFALGGYAMGMYLMRSIGRDGVYKSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LPDFMVFLDWKELPWFWHGTEHFPYALLLVVLVPGVLAWLFGFFAFRSRIKGVYLSIITQ
CCCCEEEEEHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMTYAAMLLFFRNETGFGGNNGFTDFKRIAGFAVAAPQTRAALFVLTFLALLAGFVACRY
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IVTSKLGRVVTAVRDAEMRVMFSGYNPLGYKLFVWTFSAVLCGIAGALYVPQVGIINPSE
HHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
MSPANSIEMAVWVAVGGRGTLIGPVVGAFLVNGAKTMFTAYFAEYWLFLLGAMFVLVTLY
CCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPDGVVGLWRRLRDRRTSKVETGAADEAEAVVASQQGSKA
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]