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The map label for this gene is lutR [H]

Identifier: 94309778

GI number: 94309778

Start: 910466

End: 911284

Strand: Reverse

Name: lutR [H]

Synonym: Rmet_0833

Alternate gene names: 94309778

Gene position: 911284-910466 (Counterclockwise)

Preceding gene: 94309785

Following gene: 94309777

Centisome position: 23.2

GC content: 63.37

Gene sequence:

>819_bases
ATGAGCGCATCACTACACGGTCAACAACCAGTGATCGCCACTGGCCAAGATCCAAGATATCTGCCCGCCATGTCCAATGC
CCCGCTGACTTCGCCCGCTCCCCTGCGCCGCCGTGGCCGCACGCTCGCCGAAGAGGTGGTCGGCAGCCTGACCGAGAATA
TCCAGCAGGGCCAGCTCAAACCGGGCGACAAACTCCCGACCGAATCCGAAATCATGGCCAGGCTCGGCGTGAGCCGCACG
GTGGTGCGTGAGGCGCTATCACGGCTGCAGGCCAGCGGCATGGTGGAAACGCGCCACGGCATCGGCACTTTTGTCCTGAA
TCGCCCCGCGGAAACGCAATCGCCGTTCCGCATCGACCCTTCGGCCATTGTCACGGCGATGGATGTGCTGGCAATGCTGG
AATTCCGCGTCAGCCTGGAAGCCGAAGCAGCCAGCCTGGCTGCCACGCGCCGCAGTGACGACCAACTACGGGCAATTCGC
CGGGCACTCGACGACATCAAGCACAGCGCGCTGGAGGGCAGCGATGCGGTAGGTGCCGATTTCGAGTTTCATCTGTCTAT
CGCACGCGCCACGGGCAACCGATACTTCACGGACATCATGGGCCACCTCGGCACGATGGTGATCCCGCGCAGCCGCCTGC
AAGTCAGTCAGCCCGAGCGCGAGCAGTATCTGGAACGCGTGAATTTCGAGCACGAAAGCATCTACGACGCCATCGAGCGA
CGCGACCCGGAAGCCGCCAAGGCTGCCATGCGGATGCACCTGACCAATAGCCGGGAGCGTTTGCGCAAGGCATCTGCAAT
GGCGGGATCGGCGGCCTAA

Upstream 100 bases:

>100_bases
CATTCATGTCGGTTGTCTCCGGGCCGGCGCTGCCACAACGTGAGGGTAGCTGCAAGCACCTGTGTGTTATCGGTCGTCGT
ATGACTTATGACGAAGTATA

Downstream 100 bases:

>100_bases
GGCACGCCCGTTATATGTTGCCCGCGGGGGAATCCCTACGGGCGACGCGGTTGCGGCATGCTGCGCCATGTCATATGATG
TCTTACGACAAAACCACTTC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSASLHGQQPVIATGQDPRYLPAMSNAPLTSPAPLRRRGRTLAEEVVGSLTENIQQGQLKPGDKLPTESEIMARLGVSRT
VVREALSRLQASGMVETRHGIGTFVLNRPAETQSPFRIDPSAIVTAMDVLAMLEFRVSLEAEAASLAATRRSDDQLRAIR
RALDDIKHSALEGSDAVGADFEFHLSIARATGNRYFTDIMGHLGTMVIPRSRLQVSQPEREQYLERVNFEHESIYDAIER
RDPEAAKAAMRMHLTNSRERLRKASAMAGSAA

Sequences:

>Translated_272_residues
MSASLHGQQPVIATGQDPRYLPAMSNAPLTSPAPLRRRGRTLAEEVVGSLTENIQQGQLKPGDKLPTESEIMARLGVSRT
VVREALSRLQASGMVETRHGIGTFVLNRPAETQSPFRIDPSAIVTAMDVLAMLEFRVSLEAEAASLAATRRSDDQLRAIR
RALDDIKHSALEGSDAVGADFEFHLSIARATGNRYFTDIMGHLGTMVIPRSRLQVSQPEREQYLERVNFEHESIYDAIER
RDPEAAKAAMRMHLTNSRERLRKASAMAGSAA
>Mature_271_residues
SASLHGQQPVIATGQDPRYLPAMSNAPLTSPAPLRRRGRTLAEEVVGSLTENIQQGQLKPGDKLPTESEIMARLGVSRTV
VREALSRLQASGMVETRHGIGTFVLNRPAETQSPFRIDPSAIVTAMDVLAMLEFRVSLEAEAASLAATRRSDDQLRAIRR
ALDDIKHSALEGSDAVGADFEFHLSIARATGNRYFTDIMGHLGTMVIPRSRLQVSQPEREQYLERVNFEHESIYDAIERR
DPEAAKAAMRMHLTNSRERLRKASAMAGSAA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=234, Percent_Identity=32.0512820512821, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082208, Length=225, Percent_Identity=31.5555555555556, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790032, Length=227, Percent_Identity=31.2775330396476, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789353, Length=235, Percent_Identity=29.7872340425532, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789621, Length=232, Percent_Identity=28.8793103448276, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786303, Length=220, Percent_Identity=30, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994955, Length=230, Percent_Identity=26.5217391304348, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 29927; Mature: 29796

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASLHGQQPVIATGQDPRYLPAMSNAPLTSPAPLRRRGRTLAEEVVGSLTENIQQGQLK
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGDKLPTESEIMARLGVSRTVVREALSRLQASGMVETRHGIGTFVLNRPAETQSPFRIDP
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCCCEECH
SAIVTAMDVLAMLEFRVSLEAEAASLAATRRSDDQLRAIRRALDDIKHSALEGSDAVGAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FEFHLSIARATGNRYFTDIMGHLGTMVIPRSRLQVSQPEREQYLERVNFEHESIYDAIER
EEEEEEEEHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHC
RDPEAAKAAMRMHLTNSRERLRKASAMAGSAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SASLHGQQPVIATGQDPRYLPAMSNAPLTSPAPLRRRGRTLAEEVVGSLTENIQQGQLK
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGDKLPTESEIMARLGVSRTVVREALSRLQASGMVETRHGIGTFVLNRPAETQSPFRIDP
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCCCEECH
SAIVTAMDVLAMLEFRVSLEAEAASLAATRRSDDQLRAIRRALDDIKHSALEGSDAVGAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FEFHLSIARATGNRYFTDIMGHLGTMVIPRSRLQVSQPEREQYLERVNFEHESIYDAIER
EEEEEEEEHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHC
RDPEAAKAAMRMHLTNSRERLRKASAMAGSAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]