BacMap

Definition	Cupriavidus metallidurans CH34 chromosome, complete genome.
Accession	NC_007973
Length	3,928,089

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The map label for this gene is phnL [H]

Identifier: 94309706

GI number: 94309706

Start: 839655

End: 840380

Strand: Reverse

Name: phnL [H]

Synonym: Rmet_0761

Alternate gene names: 94309706

Gene position: 840380-839655 (Counterclockwise)

Preceding gene: 94309707

Following gene: 94309705

Centisome position: 21.39

GC content: 67.22

Gene sequence:

>726_bases
ATGATGCAAGCCAACAACCAGAAACTGATTGAGGTCCGCGGCCTCGGCAAGATGTTCACGTTGCACAACCAGGGCGGCAT
TCGCCTGCCCGTGCTGCGCGCTATCGACTTTGACGCCTCGCGTGGCGAATGCCTCGTGCTGTCCGGCCGTTCCGGCACCG
GCAAGAGCACGCTTCTGCGCTGCCTCTATGGCAACTACCTCGCAACCGAAGGCACGATCCGCCTGCGCGACACGAGCGAG
GCCAGCGAGCCATGGGTGGAACTGACCAACGCCCCGGAACAACGCGTGCTCAAGTTGCGCCGCGATGTGATCGGCTATGT
CTCGCAGTTCCTGCGTGCCATCCCACGCGTCTCCGCGCTCGACGTGGTGGCCGATCCGCTGCAGCAACGCGGCGCCAGCC
ACGAGGAAGCCCGCGCCCGCGCCGCGACGCTGCTCGAACGGCTGAACCTGCCACGCCGACTTTGGGACTTGCCACCCGCC
ACGTTCTCCGGCGGCGAGCAGCAACGCGTGAATATCGCGCGCGGCCTGATCGGTGGCCACCCGATCCTGTTGCTCGACGA
GCCCACCGCGTCGCTCGACGCCGACAACCGGGCGGTGGTGATCGAACTGATCCGCGAAGCGCTGGCCGAAGGCCGCTCGC
TGATCGGCATCTTCCACGACGAGGCTGTGCGCGATGCGGTGGCCACGCGCATGCTGCCGCTGCAGCCGGCCACGGCGCTT
GCCTGA

Upstream 100 bases:

>100_bases
GCGGCCGCGTGGTCGAACAGGGCCTGACCGATCAGATCCTCGATGACCCGCAGCATCCGTACACGCAGTTGCTGGTCTCC
TCCATCCTGCAGGGATGATC

Downstream 100 bases:

>100_bases
ACCTGAACTGGAACCGGACATGTCTACCACCTACCTCACGCACGCGACGCTGGTACTGCCGGATCGCGTCCTGCACGACA
GCGCGATGCTGATCGCCGAC

Product: carbon-phosphorus lyase complex subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MMQANNQKLIEVRGLGKMFTLHNQGGIRLPVLRAIDFDASRGECLVLSGRSGTGKSTLLRCLYGNYLATEGTIRLRDTSE
ASEPWVELTNAPEQRVLKLRRDVIGYVSQFLRAIPRVSALDVVADPLQQRGASHEEARARAATLLERLNLPRRLWDLPPA
TFSGGEQQRVNIARGLIGGHPILLLDEPTASLDADNRAVVIELIREALAEGRSLIGIFHDEAVRDAVATRMLPLQPATAL
A

Sequences:

>Translated_241_residues
MMQANNQKLIEVRGLGKMFTLHNQGGIRLPVLRAIDFDASRGECLVLSGRSGTGKSTLLRCLYGNYLATEGTIRLRDTSE
ASEPWVELTNAPEQRVLKLRRDVIGYVSQFLRAIPRVSALDVVADPLQQRGASHEEARARAATLLERLNLPRRLWDLPPA
TFSGGEQQRVNIARGLIGGHPILLLDEPTASLDADNRAVVIELIREALAEGRSLIGIFHDEAVRDAVATRMLPLQPATAL
A
>Mature_241_residues
MMQANNQKLIEVRGLGKMFTLHNQGGIRLPVLRAIDFDASRGECLVLSGRSGTGKSTLLRCLYGNYLATEGTIRLRDTSE
ASEPWVELTNAPEQRVLKLRRDVIGYVSQFLRAIPRVSALDVVADPLQQRGASHEEARARAATLLERLNLPRRLWDLPPA
TFSGGEQQRVNIARGLIGGHPILLLDEPTASLDADNRAVVIELIREALAEGRSLIGIFHDEAVRDAVATRMLPLQPATAL
A

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates. Probably responsible for energy coupling to the transport system [H]

COG id: COG4778

COG function: function code P; ABC-type phosphonate transport system, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=247, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI153792144, Length=204, Percent_Identity=27.9411764705882, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI4505771, Length=198, Percent_Identity=34.3434343434343, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI9961252, Length=198, Percent_Identity=34.3434343434343, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI9961250, Length=200, Percent_Identity=33, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI21536376, Length=223, Percent_Identity=26.457399103139, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI42741659, Length=209, Percent_Identity=30.1435406698565, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI105990541, Length=190, Percent_Identity=28.9473684210526, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI47078218, Length=226, Percent_Identity=24.7787610619469, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI150417984, Length=230, Percent_Identity=25.6521739130435, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI45446740, Length=226, Percent_Identity=24.7787610619469, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1790534, Length=226, Percent_Identity=57.5221238938053, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1787029, Length=229, Percent_Identity=33.6244541484716, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786703, Length=215, Percent_Identity=38.1395348837209, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87081834, Length=216, Percent_Identity=32.4074074074074, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787089, Length=227, Percent_Identity=32.15859030837, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788225, Length=224, Percent_Identity=32.1428571428571, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787105, Length=199, Percent_Identity=33.1658291457286, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789962, Length=203, Percent_Identity=29.5566502463054, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994997, Length=205, Percent_Identity=33.1707317073171, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788644, Length=212, Percent_Identity=30.188679245283, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1787500, Length=210, Percent_Identity=32.3809523809524, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1789672, Length=219, Percent_Identity=30.5936073059361, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789873, Length=206, Percent_Identity=32.5242718446602, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790544, Length=214, Percent_Identity=34.1121495327103, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790467, Length=205, Percent_Identity=32.1951219512195, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48995001, Length=222, Percent_Identity=30.1801801801802, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786398, Length=198, Percent_Identity=31.3131313131313, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789891, Length=193, Percent_Identity=32.1243523316062, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1788761, Length=230, Percent_Identity=31.7391304347826, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693107, Length=236, Percent_Identity=30.0847457627119, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786872, Length=185, Percent_Identity=30.8108108108108, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788450, Length=219, Percent_Identity=31.0502283105023, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787712, Length=196, Percent_Identity=31.6326530612245, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI48994883, Length=207, Percent_Identity=30.9178743961353, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789032, Length=235, Percent_Identity=29.3617021276596, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787758, Length=204, Percent_Identity=27.9411764705882, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082267, Length=172, Percent_Identity=32.5581395348837, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1786698, Length=213, Percent_Identity=31.4553990610329, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1786563, Length=218, Percent_Identity=32.1100917431193, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788897, Length=217, Percent_Identity=27.1889400921659, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788506, Length=246, Percent_Identity=29.2682926829268, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081709, Length=234, Percent_Identity=29.4871794871795, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789864, Length=254, Percent_Identity=28.3464566929134, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787164, Length=206, Percent_Identity=32.0388349514563, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI2367384, Length=238, Percent_Identity=29.8319327731092, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790739, Length=200, Percent_Identity=29, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790190, Length=195, Percent_Identity=27.1794871794872, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787759, Length=206, Percent_Identity=30.0970873786408, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787112, Length=194, Percent_Identity=30.4123711340206, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787143, Length=196, Percent_Identity=30.1020408163265, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789751, Length=202, Percent_Identity=28.7128712871287, Blast_Score=60, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17558664, Length=207, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71992285, Length=199, Percent_Identity=30.6532663316583, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71984940, Length=209, Percent_Identity=30.1435406698565, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322640, Length=200, Percent_Identity=28, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319856, Length=205, Percent_Identity=31.219512195122, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24666092, Length=215, Percent_Identity=28.3720930232558, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR012701 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26456; Mature: 26456

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQANNQKLIEVRGLGKMFTLHNQGGIRLPVLRAIDFDASRGECLVLSGRSGTGKSTLLR
CCCCCCCCEEEEECCCEEEEEECCCCCEEEEEEEECCCCCCCCEEEEECCCCCCHHHHHH
CLYGNYLATEGTIRLRDTSEASEPWVELTNAPEQRVLKLRRDVIGYVSQFLRAIPRVSAL
HHHCCEEECCCEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
DVVADPLQQRGASHEEARARAATLLERLNLPRRLWDLPPATFSGGEQQRVNIARGLIGGH
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
PILLLDEPTASLDADNRAVVIELIREALAEGRSLIGIFHDEAVRDAVATRMLPLQPATAL
CEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHC
A
C
>Mature Secondary Structure
MMQANNQKLIEVRGLGKMFTLHNQGGIRLPVLRAIDFDASRGECLVLSGRSGTGKSTLLR
CCCCCCCCEEEEECCCEEEEEECCCCCEEEEEEEECCCCCCCCEEEEECCCCCCHHHHHH
CLYGNYLATEGTIRLRDTSEASEPWVELTNAPEQRVLKLRRDVIGYVSQFLRAIPRVSAL
HHHCCEEECCCEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
DVVADPLQQRGASHEEARARAATLLERLNLPRRLWDLPPATFSGGEQQRVNIARGLIGGH
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
PILLLDEPTASLDADNRAVVIELIREALAEGRSLIGIFHDEAVRDAVATRMLPLQPATAL
CEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]