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The map label for this gene is kefB [H]

Identifier: 94309253

GI number: 94309253

Start: 323463

End: 325487

Strand: Direct

Name: kefB [H]

Synonym: Rmet_0308

Alternate gene names: 94309253

Gene position: 323463-325487 (Clockwise)

Preceding gene: 94309240

Following gene: 94309256

Centisome position: 8.23

GC content: 66.72

Gene sequence:

>2025_bases
ATGCCGGGACGGCGCGCGTGCCTGGCTGGTACTTTTCAATCGCCGATGCATTCTCCGCTGGAACTGACCCTGGTGCTGCT
GGCCGCTGCCGTTTTTGGCGTGGTGGCGTTCCGCATGTTGCAACTACCGCCGATGCTCGGGTATCTGGCGGTCGGTATCC
TGATCGGCCCGCACGCGCTCGGCCTGGCCAGCGATTCGGGCCAGACCAAATACCTGGCCGAATTCGGCGTCGTCTTCCTG
ATGTTCTCGATCGGGCTCGAGTTCAGCCTGGCCAAGCTGCGCGCGATGAAGCGGCTGGTGTTCGGGCTGGGTGGATCACA
GGTCATCCTGTCGATGCTGGCTGTGGTGCCGGCGAGCTGGATGTTCAACTGGCTGTTCCCGTTGTCCTGGCAGGCATCCG
TGGCCCTGGGTGGCGCGCTGGCCATGTCCTCCACGGCCATCGTTTCCAAGATGCTTTCCGAGCGCATGGAGCTGGAAAGC
GAGCACGGGCGCAACATCATCAGCATCCTGCTGTTCCAGGATCTGGCCGTGGTGCCGCTGCTGATTGTGATCCCCGCGCT
GTCGCGCGACCCGGGAGACCTGGCGTTCGCGCTGGGGCTGGCCACGGTCAAGATCGCCGTGGCCCTGGGCGCGATCTTCT
TCCTGGGCCAGCGCCTGATGAGTCGCTGGTTCCACGTCGTGGCGGCGCGCCGGTCGCAGGAACTCTTCATGCTGAACCTG
CTGCTGGTCACGCTGGGCATGGCCGCGCTGACAGAGCGGCTGGGCCTGTCGATGGCGCTCGGCGCGTTCATGGCGGGGAT
GCTGATCTCGGAAACGCCCTACCGCCATCAGGTGGAAGAGGACATCAAGCCGTTCCGGGACGTGCTGCTCGGGCTGTTCT
TCGTCACGATCGGCATGTTGCTGAACATCCGGGTGGTGTTCGACCACGTCTGGCTCGTGCTCGCGCTGCTGATCGTGCCG
ATCCTGTTCAAGCTGGTGCTGATCGCGGTGCTGGCGCGGCTGTTCGGTTCGCGCCAGGGGGTGGCGATCCGCACCGGCCT
TGGCTTGGCGCAGGCGGGCGAATTCGGCTTCGTGCTGCTGAACCAGATCGATGGCATGGGGCTGGTGGACCCGGTGCTGA
TCCAGGTGATCCTGGCGTCGATGCTGCTGTCGATGCTGGCCGCGCCGTTCCTGATCCAGTACAGCGACGCGATCGTGCTG
CGGTTTGCCGCGAACGAATGGCTGTTGCAATCGCTGAACATGACCCGGATCGCGGCGCAGAGTCTGCAGACCGAGAAGCA
CGCGATCATCTGCGGGTACGGCCGCAGCGGCCAGAACCTGGCTCATATGCTGGAACGCGAGGGCATCACCTACGTGGCGC
TCGACCTCGACCCGGATCGCGTGCGTGACGCGGCGGCTGCGGGCGATACGGTGGTTTATGGCGATGCCGGCCGGCGCGAG
GCGCTGATCGCGGCCGGTATCCACCGCGCGGCGCTGGTGGTGGTGACTTATGCCAATACGCCATCGGCGGTGAAGGTGCT
GCACCACGTGCAGGAGCTGGCGCCGGCGCTGCCGGTGCTGGTGCGGACCGTCGATGATTCCGAACTGGACATCTTGCAGA
AGGCGGGCGCGACCGAGGTGGTGCCCGAAATCATCGAGGGCAGCCTGATGCTGGCTTCACACGCGCTGGTGCTGCTGGGC
GTGCCGATGCGCCGCGTGGTACGGGGCGTGCAGCAGGCGCGCGACGCACGCTACAGCCTGCTGCGCGGCTATTTCCACGG
CCGTGACGACGAGGAAGACATGGTGGAACGGGACACGGTGCGGCTGCACGCGGTGTCCCTCGGGCCGGAACACACGGCCG
TGGGGCGTCGCCTGGGCGCGCTGGGGCTGGAGCGGATCGGCGTGGAGGTCACGGCGGTGCGCCGGCGCGGCATCCGCGCC
TTCGACCCCGAGCCGGACACCGTGCTGGAGCCGGGCGACATCGTGGTGCTGCGTGGCACGCCCGAGGCGCTGGAGTCGGC
CGAGGAACGGCTAATCAAGGACTAG

Upstream 100 bases:

>100_bases
CGGAGTATACCAACGAATGCGATGGCGTCTGGCCCGGCTTTCCAGCCTTTTGCCGCCTTGACGACCGCCGCGCTTGCCGT
GTTTCGCCGGCAGGGCGCAA

Downstream 100 bases:

>100_bases
AACTAGACGCAGAGGCGTGCTCGCCTCTGGCTGCTCTTGGCAACGGCGAATTGCCGGCCTCCACCCACGATGCCGGCAGA
CTATCCAGCACGGCTCGCGC

Product: putative glutathione-regulated potassium-efflux system k+/h+ antiporter transmembrane protein; Kef-type K+ transport system

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 674; Mature: 673

Protein sequence:

>674_residues
MPGRRACLAGTFQSPMHSPLELTLVLLAAAVFGVVAFRMLQLPPMLGYLAVGILIGPHALGLASDSGQTKYLAEFGVVFL
MFSIGLEFSLAKLRAMKRLVFGLGGSQVILSMLAVVPASWMFNWLFPLSWQASVALGGALAMSSTAIVSKMLSERMELES
EHGRNIISILLFQDLAVVPLLIVIPALSRDPGDLAFALGLATVKIAVALGAIFFLGQRLMSRWFHVVAARRSQELFMLNL
LLVTLGMAALTERLGLSMALGAFMAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTIGMLLNIRVVFDHVWLVLALLIVP
ILFKLVLIAVLARLFGSRQGVAIRTGLGLAQAGEFGFVLLNQIDGMGLVDPVLIQVILASMLLSMLAAPFLIQYSDAIVL
RFAANEWLLQSLNMTRIAAQSLQTEKHAIICGYGRSGQNLAHMLEREGITYVALDLDPDRVRDAAAAGDTVVYGDAGRRE
ALIAAGIHRAALVVVTYANTPSAVKVLHHVQELAPALPVLVRTVDDSELDILQKAGATEVVPEIIEGSLMLASHALVLLG
VPMRRVVRGVQQARDARYSLLRGYFHGRDDEEDMVERDTVRLHAVSLGPEHTAVGRRLGALGLERIGVEVTAVRRRGIRA
FDPEPDTVLEPGDIVVLRGTPEALESAEERLIKD

Sequences:

>Translated_674_residues
MPGRRACLAGTFQSPMHSPLELTLVLLAAAVFGVVAFRMLQLPPMLGYLAVGILIGPHALGLASDSGQTKYLAEFGVVFL
MFSIGLEFSLAKLRAMKRLVFGLGGSQVILSMLAVVPASWMFNWLFPLSWQASVALGGALAMSSTAIVSKMLSERMELES
EHGRNIISILLFQDLAVVPLLIVIPALSRDPGDLAFALGLATVKIAVALGAIFFLGQRLMSRWFHVVAARRSQELFMLNL
LLVTLGMAALTERLGLSMALGAFMAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTIGMLLNIRVVFDHVWLVLALLIVP
ILFKLVLIAVLARLFGSRQGVAIRTGLGLAQAGEFGFVLLNQIDGMGLVDPVLIQVILASMLLSMLAAPFLIQYSDAIVL
RFAANEWLLQSLNMTRIAAQSLQTEKHAIICGYGRSGQNLAHMLEREGITYVALDLDPDRVRDAAAAGDTVVYGDAGRRE
ALIAAGIHRAALVVVTYANTPSAVKVLHHVQELAPALPVLVRTVDDSELDILQKAGATEVVPEIIEGSLMLASHALVLLG
VPMRRVVRGVQQARDARYSLLRGYFHGRDDEEDMVERDTVRLHAVSLGPEHTAVGRRLGALGLERIGVEVTAVRRRGIRA
FDPEPDTVLEPGDIVVLRGTPEALESAEERLIKD
>Mature_673_residues
PGRRACLAGTFQSPMHSPLELTLVLLAAAVFGVVAFRMLQLPPMLGYLAVGILIGPHALGLASDSGQTKYLAEFGVVFLM
FSIGLEFSLAKLRAMKRLVFGLGGSQVILSMLAVVPASWMFNWLFPLSWQASVALGGALAMSSTAIVSKMLSERMELESE
HGRNIISILLFQDLAVVPLLIVIPALSRDPGDLAFALGLATVKIAVALGAIFFLGQRLMSRWFHVVAARRSQELFMLNLL
LVTLGMAALTERLGLSMALGAFMAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTIGMLLNIRVVFDHVWLVLALLIVPI
LFKLVLIAVLARLFGSRQGVAIRTGLGLAQAGEFGFVLLNQIDGMGLVDPVLIQVILASMLLSMLAAPFLIQYSDAIVLR
FAANEWLLQSLNMTRIAAQSLQTEKHAIICGYGRSGQNLAHMLEREGITYVALDLDPDRVRDAAAAGDTVVYGDAGRREA
LIAAGIHRAALVVVTYANTPSAVKVLHHVQELAPALPVLVRTVDDSELDILQKAGATEVVPEIIEGSLMLASHALVLLGV
PMRRVVRGVQQARDARYSLLRGYFHGRDDEEDMVERDTVRLHAVSLGPEHTAVGRRLGALGLERIGVEVTAVRRRGIRAF
DPEPDTVLEPGDIVVLRGTPEALESAEERLIKD

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=371, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789749, Length=562, Percent_Identity=33.9857651245552, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1786232, Length=590, Percent_Identity=31.5254237288136, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1786685, Length=542, Percent_Identity=30.2583025830258, Blast_Score=197, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 73147; Mature: 73015

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGRRACLAGTFQSPMHSPLELTLVLLAAAVFGVVAFRMLQLPPMLGYLAVGILIGPHAL
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHH
GLASDSGQTKYLAEFGVVFLMFSIGLEFSLAKLRAMKRLVFGLGGSQVILSMLAVVPASW
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MFNWLFPLSWQASVALGGALAMSSTAIVSKMLSERMELESEHGRNIISILLFQDLAVVPL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LIVIPALSRDPGDLAFALGLATVKIAVALGAIFFLGQRLMSRWFHVVAARRSQELFMLNL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLVTLGMAALTERLGLSMALGAFMAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTIGML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LNIRVVFDHVWLVLALLIVPILFKLVLIAVLARLFGSRQGVAIRTGLGLAQAGEFGFVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCCCHHH
NQIDGMGLVDPVLIQVILASMLLSMLAAPFLIQYSDAIVLRFAANEWLLQSLNMTRIAAQ
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEEHHHHHHHHHCCHHHHHHH
SLQTEKHAIICGYGRSGQNLAHMLEREGITYVALDLDPDRVRDAAAAGDTVVYGDAGRRE
HHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCEEEECCCCCCH
ALIAAGIHRAALVVVTYANTPSAVKVLHHVQELAPALPVLVRTVDDSELDILQKAGATEV
HHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHH
VPEIIEGSLMLASHALVLLGVPMRRVVRGVQQARDARYSLLRGYFHGRDDEEDMVERDTV
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHE
RLHAVSLGPEHTAVGRRLGALGLERIGVEVTAVRRRGIRAFDPEPDTVLEPGDIVVLRGT
EEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCEECCCCEEEECCC
PEALESAEERLIKD
HHHHHHHHHHHCCC
>Mature Secondary Structure 
PGRRACLAGTFQSPMHSPLELTLVLLAAAVFGVVAFRMLQLPPMLGYLAVGILIGPHAL
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHH
GLASDSGQTKYLAEFGVVFLMFSIGLEFSLAKLRAMKRLVFGLGGSQVILSMLAVVPASW
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MFNWLFPLSWQASVALGGALAMSSTAIVSKMLSERMELESEHGRNIISILLFQDLAVVPL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LIVIPALSRDPGDLAFALGLATVKIAVALGAIFFLGQRLMSRWFHVVAARRSQELFMLNL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLVTLGMAALTERLGLSMALGAFMAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTIGML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LNIRVVFDHVWLVLALLIVPILFKLVLIAVLARLFGSRQGVAIRTGLGLAQAGEFGFVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCCCHHH
NQIDGMGLVDPVLIQVILASMLLSMLAAPFLIQYSDAIVLRFAANEWLLQSLNMTRIAAQ
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEEHHHHHHHHHCCHHHHHHH
SLQTEKHAIICGYGRSGQNLAHMLEREGITYVALDLDPDRVRDAAAAGDTVVYGDAGRRE
HHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCEEEECCCCCCH
ALIAAGIHRAALVVVTYANTPSAVKVLHHVQELAPALPVLVRTVDDSELDILQKAGATEV
HHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHH
VPEIIEGSLMLASHALVLLGVPMRRVVRGVQQARDARYSLLRGYFHGRDDEEDMVERDTV
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHE
RLHAVSLGPEHTAVGRRLGALGLERIGVEVTAVRRRGIRAFDPEPDTVLEPGDIVVLRGT
EEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCEECCCCEEEECCC
PEALESAEERLIKD
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA