Click here to switch to the map view.

The map label for this gene is yieH [H]

Identifier: 94309055

GI number: 94309055

Start: 117065

End: 117748

Strand: Direct

Name: yieH [H]

Synonym: Rmet_0110

Alternate gene names: 94309055

Gene position: 117065-117748 (Clockwise)

Preceding gene: 94309054

Following gene: 94309056

Centisome position: 2.98

GC content: 64.33

Gene sequence:

>684_bases
ATGACCGCAGCAAACCAGGCCGCACGCTTCGATTGCGTGATTTTCGATTGTGACGGCGTGCTCGTCGATAGCGAACCGAT
CGTGCATCGCGTGCTGAATCGCGTGCTGAACGAGATTGGTATCGAGATCACGCTCGAGGAATCCATGAAGTGGTTCCTCG
GACGCGCGGTGCGTGACGAGCTTGGCAATATCGAGGCGCGCCTGGGCAAGCCATTGCCCAGGAATTTCCTGTCCGAGTGG
TTCGTGCGTCGCGACGCGGCACTCATCGAGGAACTGGAAGCCGTGCCGCACGTCCGTCAGGCGATCGAAGCCGTCAAGGC
ACGTGGCCTGCCGGTGTGCGTGGCGTCCGGCGCCGACCGCATCAAGGTCAAGCTGCAGTTGAACCACACGCAGCTGGTGG
AACTGTTCACCGACGATGCCGCGCGCGAGCACATCTTCTCGGCTACCGAAGTGGAGCACAGCAAGCCCGCGCCCGACGTG
TACCTGCTGGCTGCGCGCACGATGGGCGTCGATCCGTCGCGCTGCGCGGTGATCGAGGACAGTCCCACCGGCGCCACGGC
GGGCGTGGCTGCCGGCATGACGGTGTTTGGCTACGCCGCGAGCACCAACGCCGACGCCTTGCGCGCGGTTGGCGTGACGA
CGATCTTCACTGACATGCGCGAGTTGCCGGGGCTGATCGGATGA

Upstream 100 bases:

>100_bases
TCGACCGCTTCGACCAGGTCGAAGCCTATCTGAAGAGCCGGCGGCAGACCGAACTGCGCGCCGTCGGCGGTAACGAACTC
AACAAGGAAAAGAAACCCGC

Downstream 100 bases:

>100_bases
CGAACAAGGGTCGGGCCGGCACCGGCCCGGCACCGTGCCCCTGCGGCAACGGCGCCTACGACACGTGCTGCGGCCGCTTC
CATCGTGGCGAGGCATCGCC

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTAANQAARFDCVIFDCDGVLVDSEPIVHRVLNRVLNEIGIEITLEESMKWFLGRAVRDELGNIEARLGKPLPRNFLSEW
FVRRDAALIEELEAVPHVRQAIEAVKARGLPVCVASGADRIKVKLQLNHTQLVELFTDDAAREHIFSATEVEHSKPAPDV
YLLAARTMGVDPSRCAVIEDSPTGATAGVAAGMTVFGYAASTNADALRAVGVTTIFTDMRELPGLIG

Sequences:

>Translated_227_residues
MTAANQAARFDCVIFDCDGVLVDSEPIVHRVLNRVLNEIGIEITLEESMKWFLGRAVRDELGNIEARLGKPLPRNFLSEW
FVRRDAALIEELEAVPHVRQAIEAVKARGLPVCVASGADRIKVKLQLNHTQLVELFTDDAAREHIFSATEVEHSKPAPDV
YLLAARTMGVDPSRCAVIEDSPTGATAGVAAGMTVFGYAASTNADALRAVGVTTIFTDMRELPGLIG
>Mature_226_residues
TAANQAARFDCVIFDCDGVLVDSEPIVHRVLNRVLNEIGIEITLEESMKWFLGRAVRDELGNIEARLGKPLPRNFLSEWF
VRRDAALIEELEAVPHVRQAIEAVKARGLPVCVASGADRIKVKLQLNHTQLVELFTDDAAREHIFSATEVEHSKPAPDVY
LLAARTMGVDPSRCAVIEDSPTGATAGVAAGMTVFGYAASTNADALRAVGVTTIFTDMRELPGLIG

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=219, Percent_Identity=31.0502283105023, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1789046, Length=191, Percent_Identity=30.3664921465969, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1788021, Length=182, Percent_Identity=33.5164835164835, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787576, Length=202, Percent_Identity=28.2178217821782, Blast_Score=61, Evalue=6e-11,
Organism=Drosophila melanogaster, GI20129151, Length=223, Percent_Identity=29.1479820627803, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137324, Length=198, Percent_Identity=29.2929292929293, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI116008157, Length=190, Percent_Identity=32.6315789473684, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI45550911, Length=188, Percent_Identity=26.5957446808511, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24652; Mature: 24520

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAANQAARFDCVIFDCDGVLVDSEPIVHRVLNRVLNEIGIEITLEESMKWFLGRAVRDE
CCCCCCCCEEEEEEEECCCEEECCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHH
LGNIEARLGKPLPRNFLSEWFVRRDAALIEELEAVPHVRQAIEAVKARGLPVCVASGADR
HCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCE
IKVKLQLNHTQLVELFTDDAAREHIFSATEVEHSKPAPDVYLLAARTMGVDPSRCAVIED
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCCCCCEEEEEC
SPTGATAGVAAGMTVFGYAASTNADALRAVGVTTIFTDMRELPGLIG
CCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TAANQAARFDCVIFDCDGVLVDSEPIVHRVLNRVLNEIGIEITLEESMKWFLGRAVRDE
CCCCCCCEEEEEEEECCCEEECCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHH
LGNIEARLGKPLPRNFLSEWFVRRDAALIEELEAVPHVRQAIEAVKARGLPVCVASGADR
HCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCE
IKVKLQLNHTQLVELFTDDAAREHIFSATEVEHSKPAPDVYLLAARTMGVDPSRCAVIED
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCCCCCEEEEEC
SPTGATAGVAAGMTVFGYAASTNADALRAVGVTTIFTDMRELPGLIG
CCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]