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The map label for this gene is doxJ [H]

Identifier: 94309054

GI number: 94309054

Start: 116409

End: 117068

Strand: Direct

Name: doxJ [H]

Synonym: Rmet_0109

Alternate gene names: 94309054

Gene position: 116409-117068 (Clockwise)

Preceding gene: 94309053

Following gene: 94309055

Centisome position: 2.96

GC content: 60.61

Gene sequence:

>660_bases
ATGACCGCCCCCATCGACTTCTACTTCGATTTCTCCTCGCCCTATGGCTACTTCGCCAGCACGCGCATCGACGATATTGC
CCAGAAGTACGGCCGCTATGTGATCTGGCATCCGATCCTGCTCGGCGTGGTGTTCAAGACAACCGGCTCGTCGCCGCTTC
CCAGCATTCCGCTCAAGGGCGATTACTGCTGGCGTGATTTCGAGCGGACCTCGGAATTCAACCATATCGACTACAAGCGG
CCCACGCATTTCCCACTGCCGACGCGACATGCAGCGCGCGCGATGCTATGGCTGCAGAACAACCACGGTGCGGATGTGGC
CACGGCGTTTGCCAAGGCCGTGTATCGCGCGCTGTTTGTCGACGACATCAACATTGCCGAACCCGCCGAACTGGCGAAGC
TGGCCGAGCCGCTCGGCGTCGACCCGATCGCCATGAACGAAGGTGCCAACAACTACCAGATCAAGGACCAGCTCAAGGCC
GAGATCGACGTGGCGATGGCCAAGGGTGTGTTCGGCTCGCCGTTCGTGATCGTCGACGGCGAACCGTTCTGGGGCTTCGA
CCGCTTCGACCAGGTCGAAGCCTATCTGAAGAGCCGGCGGCAGACCGAACTGCGCGCCGTCGGCGGTAACGAACTCAACA
AGGAAAAGAAACCCGCATGA

Upstream 100 bases:

>100_bases
CGCTTGGCTGACAGGCATCTCGAATACGAAGACGAACACAAGTACGAACACGAGTATCCGCACTTTCCTCAACTGTTGTC
CGTGAACCGGAGCCTTTGCG

Downstream 100 bases:

>100_bases
CCGCAGCAAACCAGGCCGCACGCTTCGATTGCGTGATTTTCGATTGTGACGGCGTGCTCGTCGATAGCGAACCGATCGTG
CATCGCGTGCTGAATCGCGT

Product: 2-hydroxychromene-2-carboxylate isomerase

Products: NA

Alternate protein names: HCCA isomerase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTAPIDFYFDFSSPYGYFASTRIDDIAQKYGRYVIWHPILLGVVFKTTGSSPLPSIPLKGDYCWRDFERTSEFNHIDYKR
PTHFPLPTRHAARAMLWLQNNHGADVATAFAKAVYRALFVDDINIAEPAELAKLAEPLGVDPIAMNEGANNYQIKDQLKA
EIDVAMAKGVFGSPFVIVDGEPFWGFDRFDQVEAYLKSRRQTELRAVGGNELNKEKKPA

Sequences:

>Translated_219_residues
MTAPIDFYFDFSSPYGYFASTRIDDIAQKYGRYVIWHPILLGVVFKTTGSSPLPSIPLKGDYCWRDFERTSEFNHIDYKR
PTHFPLPTRHAARAMLWLQNNHGADVATAFAKAVYRALFVDDINIAEPAELAKLAEPLGVDPIAMNEGANNYQIKDQLKA
EIDVAMAKGVFGSPFVIVDGEPFWGFDRFDQVEAYLKSRRQTELRAVGGNELNKEKKPA
>Mature_218_residues
TAPIDFYFDFSSPYGYFASTRIDDIAQKYGRYVIWHPILLGVVFKTTGSSPLPSIPLKGDYCWRDFERTSEFNHIDYKRP
THFPLPTRHAARAMLWLQNNHGADVATAFAKAVYRALFVDDINIAEPAELAKLAEPLGVDPIAMNEGANNYQIKDQLKAE
IDVAMAKGVFGSPFVIVDGEPFWGFDRFDQVEAYLKSRRQTELRAVGGNELNKEKKPA

Specific function: Catalyzes the isomerization of 2-hydroxychromene-2- carboxylate (HCCA) to trans-O-hydroxybenzylidenepyruvate (THBPA). The reaction is reversible [H]

COG id: COG3917

COG function: function code Q; 2-hydroxychromene-2-carboxylate isomerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR014440
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 24755; Mature: 24624

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPIDFYFDFSSPYGYFASTRIDDIAQKYGRYVIWHPILLGVVFKTTGSSPLPSIPLKG
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCCCCCCCCCCC
DYCWRDFERTSEFNHIDYKRPTHFPLPTRHAARAMLWLQNNHGADVATAFAKAVYRALFV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHH
DDINIAEPAELAKLAEPLGVDPIAMNEGANNYQIKDQLKAEIDVAMAKGVFGSPFVIVDG
CCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECC
EPFWGFDRFDQVEAYLKSRRQTELRAVGGNELNKEKKPA
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TAPIDFYFDFSSPYGYFASTRIDDIAQKYGRYVIWHPILLGVVFKTTGSSPLPSIPLKG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCCCCCCCCCCC
DYCWRDFERTSEFNHIDYKRPTHFPLPTRHAARAMLWLQNNHGADVATAFAKAVYRALFV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHH
DDINIAEPAELAKLAEPLGVDPIAMNEGANNYQIKDQLKAEIDVAMAKGVFGSPFVIVDG
CCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECC
EPFWGFDRFDQVEAYLKSRRQTELRAVGGNELNKEKKPA
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226631 [H]