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The map label for this gene is aceK

Identifier: 94309049

GI number: 94309049

Start: 110726

End: 112549

Strand: Direct

Name: aceK

Synonym: Rmet_0104

Alternate gene names: 94309049

Gene position: 110726-112549 (Clockwise)

Preceding gene: 94309048

Following gene: 94309050

Centisome position: 2.82

GC content: 60.75

Gene sequence:

>1824_bases
ATGTCCCACTTCCCCAAGCTGCTCTCGTCGCAGATTGCCTACGATGTGGCGCGGACCATGCTGGATGGCTTCGACAAGCA
CTACCGGCTGTTCCGCGAAGTCAGTATCGAGGCCAAGGCGCGCTTCGAGGTCGGTGACTGGCATGGTCTGCAGCAACTGC
AGCGGGACCGCATCGCGTTCTACAACCAGCGCGTGCACGAAACCATCGTGACGCTCCAGGACGAGTACGACGCGGAGGAC
ATCGAGGACGAGATCTGGCAGCAGATCAAGCTGCACTACATCGGCCTGCTGACGAACCATCATCAGCCCGAACTGGCCGA
GACGTTCTTCAACTCGGTCTTCACGCGTATCCAGCACCGCTCGTACTTCAACAACGATTTCATCTTCGTGCGTCCCGCGA
TCTCGACCGAGTACATCGAGAACGAGGAGTCGCCGATCAAGCCGACCTACCGCGCCTACTACCCGGGCAGCCGCGAGGGC
ATGGCGGCCTGCTTCGAGCGGATCGTGAACAACTTTCAGCTCGAGCGTCCGTTCGAGGATCTCAAGCGGGATGCCGGCTA
TGTGGCCCGCGCGATCACCGAGCACTTCGGCGAATTCCGCATTGCGCCGAATTTCCAGGTGCATACGCTGTCGTCGCTGT
TCTTCCGCAACAAGACTGCGTTCGTCATCGGCCGGGTGATCAACGGCGACCATACCTATCCGCTGGTGGTGCCGATCATT
CACGCGGCATCTGGCAAGCTCGTGCTGGACACGGTGCTGCTGCACCGGGAGCAGATCCTGATCCTGTTCTCGTTCGCGCA
CGCCTACTTCATGGTCGACATGGAGATCCCGTCGGCCTACGTGACTTTCCTGCGAGACCTGCTGCCGCGCAAGTCGCGCG
CGGAGATCTACACCTCGCTGGGTTTGCAGAAGCAGGGCAAGAACCTGTTCTATCGCGACTTCCTGCATCATCTGCAGCAT
TCGTCGGATAAATTCATCAGCGCGCCGGGCATCAAGGGGCTGGTGATGCTGGTGTTCACGCTGCCGTCGTTCCCATACGT
GTTCAAGGTGATCCGCGATTTCTTCCCGGCGCCGAAGGAAACCACGCGCGAACTGGTGAAATCGAAATACCAGCTCGTCA
AGCAGCATGACCGCGTGGGCCGCATGGCCGATACGCTCGAGTACTCCGATGTGGCGTTCCCGCTGTCCCGCTTCGACGAC
GCGCTGGTGCGCGAGCTGGAGCAGCACGCGCCGTCGATGATCGAGTACCAGCGCAGCAAGCAGGGCGAGGACGAGATCGT
CGTGCGCCATGTCTATATCGAACGCCGCATGACGCCGCTGAACATCTGGCTTCAGGAGGGAACGGACGAGCAGGTGGATC
ACGGCATCCTCGAATACGGCAACGCCGTCAAGGAACTGATCGCCGCGAACATCTTCCCCGGCGACATGCTTTACAAGAAC
TTCGGCGTAACCCGCCACGGCCGCGTGGTCTTCTACGACTACGACGAGATCGAGTACCTGACCGACTGCAACGTCCGCCG
CGTACCCGCCCCGCGCAACGAGGAAGAGGAAATGTCCGGCGAGGTGTGGTACACCGTACGTCCGCACGACATTTTTCCGG
AGACCTACGGCACATTCCTGCTGGGCGATCCGCGTGTGCGCGCCGCCTTCATGCGGCATCACCCCGATTTCTTTGACGCT
GCGATGTGGCAGCACCACAAAGACCGGCTCCTGGCCGGCCATGTCCATGACTTTTTTGCGTACCACAGCCAAGAACGCTT
CATCCACCGCTACGGGTCCGGCGCAACCGGCCCCGCAACTGAAGAACCAAGGAGGGCAGCATGA

Upstream 100 bases:

>100_bases
TATGAGGCGTTCGGTGCCGCCATTGGGCCGGCACCGAACGCCTTTACAATCTCAACATCGCCGCCTGCCCGATTCTCACA
GTCTTCGAGCCCTCCGAGCC

Downstream 100 bases:

>100_bases
GTGACGCCATCGCCCAGCTGTTCCGCAACAACCGCGAATGGGTAGACCGCGTCAACGCGGAAGACCCGACTTTCTTCACG
CGCCTGGCCAACCAGCAGGC

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P

Number of amino acids: Translated: 607; Mature: 606

Protein sequence:

>607_residues
MSHFPKLLSSQIAYDVARTMLDGFDKHYRLFREVSIEAKARFEVGDWHGLQQLQRDRIAFYNQRVHETIVTLQDEYDAED
IEDEIWQQIKLHYIGLLTNHHQPELAETFFNSVFTRIQHRSYFNNDFIFVRPAISTEYIENEESPIKPTYRAYYPGSREG
MAACFERIVNNFQLERPFEDLKRDAGYVARAITEHFGEFRIAPNFQVHTLSSLFFRNKTAFVIGRVINGDHTYPLVVPII
HAASGKLVLDTVLLHREQILILFSFAHAYFMVDMEIPSAYVTFLRDLLPRKSRAEIYTSLGLQKQGKNLFYRDFLHHLQH
SSDKFISAPGIKGLVMLVFTLPSFPYVFKVIRDFFPAPKETTRELVKSKYQLVKQHDRVGRMADTLEYSDVAFPLSRFDD
ALVRELEQHAPSMIEYQRSKQGEDEIVVRHVYIERRMTPLNIWLQEGTDEQVDHGILEYGNAVKELIAANIFPGDMLYKN
FGVTRHGRVVFYDYDEIEYLTDCNVRRVPAPRNEEEEMSGEVWYTVRPHDIFPETYGTFLLGDPRVRAAFMRHHPDFFDA
AMWQHHKDRLLAGHVHDFFAYHSQERFIHRYGSGATGPATEEPRRAA

Sequences:

>Translated_607_residues
MSHFPKLLSSQIAYDVARTMLDGFDKHYRLFREVSIEAKARFEVGDWHGLQQLQRDRIAFYNQRVHETIVTLQDEYDAED
IEDEIWQQIKLHYIGLLTNHHQPELAETFFNSVFTRIQHRSYFNNDFIFVRPAISTEYIENEESPIKPTYRAYYPGSREG
MAACFERIVNNFQLERPFEDLKRDAGYVARAITEHFGEFRIAPNFQVHTLSSLFFRNKTAFVIGRVINGDHTYPLVVPII
HAASGKLVLDTVLLHREQILILFSFAHAYFMVDMEIPSAYVTFLRDLLPRKSRAEIYTSLGLQKQGKNLFYRDFLHHLQH
SSDKFISAPGIKGLVMLVFTLPSFPYVFKVIRDFFPAPKETTRELVKSKYQLVKQHDRVGRMADTLEYSDVAFPLSRFDD
ALVRELEQHAPSMIEYQRSKQGEDEIVVRHVYIERRMTPLNIWLQEGTDEQVDHGILEYGNAVKELIAANIFPGDMLYKN
FGVTRHGRVVFYDYDEIEYLTDCNVRRVPAPRNEEEEMSGEVWYTVRPHDIFPETYGTFLLGDPRVRAAFMRHHPDFFDA
AMWQHHKDRLLAGHVHDFFAYHSQERFIHRYGSGATGPATEEPRRAA
>Mature_606_residues
SHFPKLLSSQIAYDVARTMLDGFDKHYRLFREVSIEAKARFEVGDWHGLQQLQRDRIAFYNQRVHETIVTLQDEYDAEDI
EDEIWQQIKLHYIGLLTNHHQPELAETFFNSVFTRIQHRSYFNNDFIFVRPAISTEYIENEESPIKPTYRAYYPGSREGM
AACFERIVNNFQLERPFEDLKRDAGYVARAITEHFGEFRIAPNFQVHTLSSLFFRNKTAFVIGRVINGDHTYPLVVPIIH
AASGKLVLDTVLLHREQILILFSFAHAYFMVDMEIPSAYVTFLRDLLPRKSRAEIYTSLGLQKQGKNLFYRDFLHHLQHS
SDKFISAPGIKGLVMLVFTLPSFPYVFKVIRDFFPAPKETTRELVKSKYQLVKQHDRVGRMADTLEYSDVAFPLSRFDDA
LVRELEQHAPSMIEYQRSKQGEDEIVVRHVYIERRMTPLNIWLQEGTDEQVDHGILEYGNAVKELIAANIFPGDMLYKNF
GVTRHGRVVFYDYDEIEYLTDCNVRRVPAPRNEEEEMSGEVWYTVRPHDIFPETYGTFLLGDPRVRAAFMRHHPDFFDAA
MWQHHKDRLLAGHVHDFFAYHSQERFIHRYGSGATGPATEEPRRAA

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family

Homologues:

Organism=Escherichia coli, GI1790446, Length=578, Percent_Identity=42.2145328719723, Blast_Score=461, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACEK_RALME (Q1LS86)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582259.1
- STRING:   Q1LS86
- GeneID:   4036888
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0104
- eggNOG:   COG4579
- HOGENOM:   HBG298593
- OMA:   RMTPLNI
- PhylomeDB:   Q1LS86
- ProtClustDB:   PRK02946
- BioCyc:   RMET266264:RMET_0104-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00747
- InterPro:   IPR010452
- PIRSF:   PIRSF000719
- ProDom:   PD043552

Pfam domain/function: PF06315 AceK

EC number: =2.7.11.5

Molecular weight: Translated: 71111; Mature: 70979

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: ACT_SITE 384-384 BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHFPKLLSSQIAYDVARTMLDGFDKHYRLFREVSIEAKARFEVGDWHGLQQLQRDRIAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCHHHHHHHHHHHHHH
YNQRVHETIVTLQDEYDAEDIEDEIWQQIKLHYIGLLTNHHQPELAETFFNSVFTRIQHR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SYFNNDFIFVRPAISTEYIENEESPIKPTYRAYYPGSREGMAACFERIVNNFQLERPFED
HCCCCCEEEEECCCCHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHH
LKRDAGYVARAITEHFGEFRIAPNFQVHTLSSLFFRNKTAFVIGRVINGDHTYPLVVPII
HHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCHHEEEH
HAASGKLVLDTVLLHREQILILFSFAHAYFMVDMEIPSAYVTFLRDLLPRKSRAEIYTSL
HCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHH
GLQKQGKNLFYRDFLHHLQHSSDKFISAPGIKGLVMLVFTLPSFPYVFKVIRDFFPAPKE
CCHHCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
TTRELVKSKYQLVKQHDRVGRMADTLEYSDVAFPLSRFDDALVRELEQHAPSMIEYQRSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHC
QGEDEIVVRHVYIERRMTPLNIWLQEGTDEQVDHGILEYGNAVKELIAANIFPGDMLYKN
CCCCHHEEEEEHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FGVTRHGRVVFYDYDEIEYLTDCNVRRVPAPRNEEEEMSGEVWYTVRPHDIFPETYGTFL
CCCCCCCEEEEEECCCHHHHHCCCCEECCCCCCCHHHCCCCEEEEECCCCCCCCCCCCEE
LGDPRVRAAFMRHHPDFFDAAMWQHHKDRLLAGHVHDFFAYHSQERFIHRYGSGATGPAT
ECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EEPRRAA
CCCCCCC
>Mature Secondary Structure 
SHFPKLLSSQIAYDVARTMLDGFDKHYRLFREVSIEAKARFEVGDWHGLQQLQRDRIAF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCHHHHHHHHHHHHHH
YNQRVHETIVTLQDEYDAEDIEDEIWQQIKLHYIGLLTNHHQPELAETFFNSVFTRIQHR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SYFNNDFIFVRPAISTEYIENEESPIKPTYRAYYPGSREGMAACFERIVNNFQLERPFED
HCCCCCEEEEECCCCHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHH
LKRDAGYVARAITEHFGEFRIAPNFQVHTLSSLFFRNKTAFVIGRVINGDHTYPLVVPII
HHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCHHEEEH
HAASGKLVLDTVLLHREQILILFSFAHAYFMVDMEIPSAYVTFLRDLLPRKSRAEIYTSL
HCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHH
GLQKQGKNLFYRDFLHHLQHSSDKFISAPGIKGLVMLVFTLPSFPYVFKVIRDFFPAPKE
CCHHCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
TTRELVKSKYQLVKQHDRVGRMADTLEYSDVAFPLSRFDDALVRELEQHAPSMIEYQRSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHC
QGEDEIVVRHVYIERRMTPLNIWLQEGTDEQVDHGILEYGNAVKELIAANIFPGDMLYKN
CCCCHHEEEEEHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FGVTRHGRVVFYDYDEIEYLTDCNVRRVPAPRNEEEEMSGEVWYTVRPHDIFPETYGTFL
CCCCCCCEEEEEECCCHHHHHCCCCEECCCCCCCHHHCCCCEEEEECCCCCCCCCCCCEE
LGDPRVRAAFMRHHPDFFDAAMWQHHKDRLLAGHVHDFFAYHSQERFIHRYGSGATGPAT
ECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EEPRRAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA