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The map label for this gene is dnaN [H]

Identifier: 94308947

GI number: 94308947

Start: 2220

End: 3335

Strand: Direct

Name: dnaN [H]

Synonym: Rmet_0002

Alternate gene names: 94308947

Gene position: 2220-3335 (Clockwise)

Preceding gene: 94308946

Following gene: 94308948

Centisome position: 0.06

GC content: 60.3

Gene sequence:

>1116_bases
ATGCAATTGGTCAAAACCTCGCGAGACAATCTGCTGCGTCCGCTGCAAATCGTGAGCGGCATCGTGGAGCGCCGCCACAC
CCTCCCGATCCTGGCCAACCTGCTGATTCGCAAGTCCGGCTCGAACGTATCCTTCCTCTCGACCGACATCGAGATCCAGA
TCACGACCCATGCCGAATGCGGTGTCGGTAGCGACAGCGTGGCGACCACCGTGGCCGCGCGCAAGCTGCTGGACATCCTG
CGCGCGATGCCTGACGGCGACGTGGCGCTGTCGCTGAACGACAAGCGCATGACCGTGCAGTCGGGCAAGAGCAGGTTTGC
GCTGCAGACGCTGGCCGCGGAAGAATTCCCGACCGTTGCCGAAGCCGCCGAATTCAACGCCAGCGTGAGCCTGCCGCAGA
AGACGTTCAAGCATCTGCTGGCGATGGTCCACTTCGCCATGGCGCAGCAGGACATCCGTTACTACCTGAACGGGATGCTG
CTGGTGGTCGATGGCAAGAAGGTCATGGCCGTGGCGACCGACGGTCACCGCCTGGCGTACTGCGGCGTGGAGCTCGAGAA
TGAAGCGACCGGCGTCGGTTCGCGCCAGGAAGTGATCATCCCGCGCAAGACCATCCTGGAACTGCAACGCCTGCTGGAAG
ACACCGATGATCCCGTGCAGGTGCAACTGGCCGCCAATCAGGTCAAGTTCACCTTCGCCAATATCGAACTGATCAGCAAG
CTGGTGGAAGGCAAGTTCCCGGACTTCCAGCGCGTGATCCCGAAGGGCTACAAGAACGCGTTTGCGATCGACCGCGTGCA
ACTGCAACAGGCGCTGCAACGCACGGCGATTCTGACTACCGACAAGTTCAAGGGCGTGCGTTGCATTCTCGATACGCACG
TGCTGAAGATCAGCTCCACCAACGCCGATCAGGAAGAGGCGCAGGAAGAGCTGGAACTCGATTATTCGGGCGATGCGCTC
GATATCGGCTTTAACGTGACCTATCTGCTCGATGTGCTCGCCAACCTGAAGAGCGAGCAGGTGCAGGTCAGCCTCGGCGA
CTCCAATTCGAGCGCGCTGATTACGGTGCCGGAAGACGACAACTTCAAGTATGTCGTCATGCCGATGCGCATTTGA

Upstream 100 bases:

>100_bases
TCCTTGGTACAATGCGACATTAACTCCTTGATTCCTAAGTGAATTTGGAGTTGTGCGAAACCCTGCGGTCGCTATCGACA
AACGACGAAGGATAAATTCA

Downstream 100 bases:

>100_bases
TGGCCACCATCAGACGCCATCCAGACCACAATCGGGCACACACCGCAGCAGGGGCCGGATCGAACCGATCCGCCCCTTTT
GCCGTTTTTAAGTAACCCGC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAECGVGSDSVATTVAARKLLDIL
RAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVAEAAEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGML
LVVDGKKVMAVATDGHRLAYCGVELENEATGVGSRQEVIIPRKTILELQRLLEDTDDPVQVQLAANQVKFTFANIELISK
LVEGKFPDFQRVIPKGYKNAFAIDRVQLQQALQRTAILTTDKFKGVRCILDTHVLKISSTNADQEEAQEELELDYSGDAL
DIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDDNFKYVVMPMRI

Sequences:

>Translated_371_residues
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAECGVGSDSVATTVAARKLLDIL
RAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVAEAAEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGML
LVVDGKKVMAVATDGHRLAYCGVELENEATGVGSRQEVIIPRKTILELQRLLEDTDDPVQVQLAANQVKFTFANIELISK
LVEGKFPDFQRVIPKGYKNAFAIDRVQLQQALQRTAILTTDKFKGVRCILDTHVLKISSTNADQEEAQEELELDYSGDAL
DIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDDNFKYVVMPMRI
>Mature_371_residues
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAECGVGSDSVATTVAARKLLDIL
RAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVAEAAEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGML
LVVDGKKVMAVATDGHRLAYCGVELENEATGVGSRQEVIIPRKTILELQRLLEDTDDPVQVQLAANQVKFTFANIELISK
LVEGKFPDFQRVIPKGYKNAFAIDRVQLQQALQRTAILTTDKFKGVRCILDTHVLKISSTNADQEEAQEELELDYSGDAL
DIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDDNFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=365, Percent_Identity=43.2876712328767, Blast_Score=305, Evalue=4e-84,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41035; Mature: 41035

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAEC
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCC
GVGSDSVATTVAARKLLDILRAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVA
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHCCCCHH
EAAEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGMLLVVDGKKVMAVATDGHRLAY
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE
CGVELENEATGVGSRQEVIIPRKTILELQRLLEDTDDPVQVQLAANQVKFTFANIELISK
EEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEECEEEEEEHHHHHHHH
LVEGKFPDFQRVIPKGYKNAFAIDRVQLQQALQRTAILTTDKFKGVRCILDTHVLKISST
HHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEEEEEEECC
NADQEEAQEELELDYSGDALDIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDD
CCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCC
NFKYVVMPMRI
CEEEEEEEECC
>Mature Secondary Structure
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAEC
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCC
GVGSDSVATTVAARKLLDILRAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVA
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHCCCCHH
EAAEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGMLLVVDGKKVMAVATDGHRLAY
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE
CGVELENEATGVGSRQEVIIPRKTILELQRLLEDTDDPVQVQLAANQVKFTFANIELISK
EEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEECEEEEEEHHHHHHHH
LVEGKFPDFQRVIPKGYKNAFAIDRVQLQQALQRTAILTTDKFKGVRCILDTHVLKISST
HHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEEEEEEECC
NADQEEAQEELELDYSGDALDIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDD
CCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCC
NFKYVVMPMRI
CEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]