Click here to switch to the map view.

The map label for this gene is gyrB [H]

Identifier: 94308948

GI number: 94308948

Start: 3521

End: 6046

Strand: Direct

Name: gyrB [H]

Synonym: Rmet_0003

Alternate gene names: 94308948

Gene position: 3521-6046 (Clockwise)

Preceding gene: 94308947

Following gene: 94308949

Centisome position: 0.09

GC content: 63.14

Gene sequence:

>2526_bases
ATGACCGATCAGCAGAAACCGCAGCAGGAAAACTCTTACGGCGCCTCTTCGATCCAGATCCTGGAAGGCCTGGAGGCGGT
ACGCAAGCGTCCCGGCATGTACATCGGCGATACGTCCGACGGCACCGGCTTGCACCACCTCGTGTTCGAGGTACTCGACA
ACTCGATCGACGAAGCCCTGGCCGGTTATTGCACCGAGATCCAGGTCACGATCCACAGCGACAACTCGATCTCGATCGTC
GACAACGGCCGCGGCATTCCGCCGGCCGTGAAGTTCGACGACAAGCACGAGCCGAAGCGCAGCGCGGCGGAAATCGCCAT
GACCGAACTGCACGCCGGCGGCAAGTTCAACCAGAACAGCTACAAGGTTTCGGGCGGCCTGCATGGCGTGGGCGTGTCCT
GCGTGAACGCGCTGTCGAAGTGGCTGCGCCTGACCGTGCGCCGCGATGGCCAGGTCAACCTGATCGAGTTCGCGAAGGGT
GAAGTACAGAACCGCGTTATCGAGACGGTGACCGGCCCGGACGGCCAGCCCGTTGAAGTGTCGCCGATGAAGGTCATCGG
TGCGACCGACAAGCGCGGCACCGAAGTGCACTTCCTGGCCGACGAGGAAATCTTCACGAACGTCGAGTTCCACTACGAAG
TGCTGTCCAAGCGCATCCGCGAACTCTCGTTCCTGAACAACGGCGTGCATATCAAGCTGGTGGACCAGCGCACGGGCAAG
GAAGAGGACTTCGCGTTCTCCGGTGGCGTGAAGGGTTTCGTCGAGTACATCAACCGCTCGAAGACCACGCTGCACCCGAA
CATTTTCTACGCGAACGCCGAGAAGGATGGCATCAGCGTGGAAGTGGCCATGCAGTGGAACGATGGCTACAACGAGCAGG
TGCTCTGCTTCACGAACAACATTCCGCAGCGTGACGGCGGTACCCACCTGACCGGCTTGCGCGCGGCGATGACCCGTGTC
ATCAACAAGTACATCGAAGAAAACGAGGTCGCCAAGAAGGCGAAGGTCGAAACCTCCGGCGACGACATGCGCGAAGGCCT
GGCCTGCGTGCTGTCCGTGAAGGTACCCGAGCCGAAGTTCAGCTCGCAGACCAAGGACAAGCTCGTTTCCTCCGAAGTGC
GCCTGCCTGTGGAAGAGCTCGTCGGCAAGGCGCTGACCGACTTCCTGCTGGAAACGCCGAACGACGCCAAGATCATCTGC
GGCAAGATCGTCGATGCGGCCCGCGCCCGCGAAGCCGCGCGCAAGGCCCGCGAAATGACGCGCCGCAAGGGCGTCATGGA
CGGCATGGGCCTGCCCGGCAAGCTCGCGGACTGCCAGGAGAAGGATCCGGCGATGTCCGAGCTGTTCCTCGTCGAGGGTG
ACTCCGCAGGCGGCTCCGCCAAGCAGGGCCGCGACCGCAAGTTCCAGGCGATCCTGCCGCTCAAGGGCAAGATCCTGAAC
GTGGAACGCGCGCGCTTCGACAAGATGCTGTCGAGCCAGGAAGTGCTGACGCTGATCACGGCACTGGGCACCGGCATCGG
CAAGGAGGACTACAACCTCGAGAAGCTGCGCTACCACCGCATCATCATCATGACCGATGCGGACGTGGACGGCTCGCACA
TCCGCACACTGCTGCTGACGTTCTTCTATCGCCAGATGCCGGACATCATCGAGCGCGGCTACGTGTACATCGCCCAGCCG
CCGCTCTACAAGATCAAGCACGGCAAGGAAGAGCGCTACATCAAGGACGACAACGAGCTGAACGCCTACATGCTGAAGCT
GGCAATGGAAAAGGCTTCGCTCAGCCGCCCGGATGGCAGCGAGATCAACGGTGAAGCCCTGACCGAACTGGCACGCCAGT
ACCAGCTCGCCGAAGGCGTGATCGGCCGCCTGTCGCGCATCGTCGATGTGGACGCGCTACGCGCGATCGCCGACGGCGTG
GCGCTCGACCTCGACAACGCCGCCGCAGCCGAAGCCTCGGCCGTGGCGCTGAAGGCCAAGCTCGCCGAGATGCACGCCAA
GACGCTGCTCGGCGCCGCGGTCAACGACGACGGCACCGCCGACGTCTATGCCCAGTTCGACGAGAAGACCGACAAGCACC
GCCTGATGATCGCGCGTCGCCATCATGGCAACGTGCGTCTGTCACACGTGGATGCGGACTTCGTCCACGGCGCCGACTAC
GCGGCACTCGCCCGCGCGGCCACGACCTTCCAGGGCCTGATCCCGGACGGCACCAAGGTTCGCCGCGGCGAAGGCGAGAA
GATGCGCGAACAGACCGTCGCAGACTTCCACCAGGCCATGCAGTGGCTACTCTCCGAAGCCGAACGCGGCGTCTCGCGCC
AGCGCTACAAGGGCCTGGGCGAAATGAACCCCGAGCAGCTCTTCGAAACCACGCTCGACGTCACGCAGCGCCGCCTGCTG
AGGGTCCAGATCGAAGACGCCATCGCCGCCGACCAGATCTTCACGACACTGATGGGCGACGAGGTGGAACCGCGCCGGAA
CTTTATCGAGTCGAATGCGCTGGTGGCTCGGAATATTGATGTTTGA

Upstream 100 bases:

>100_bases
TTTTAAGTAACCCGCGAAACGCGGATCTACCGCACCCGCCGCATTTTTGGCGCAGGTTCGGTTTTCCGCTTTTTGCCGTC
AGTATCGTGAGTAGAAAGAC

Downstream 100 bases:

>100_bases
TTGTGTCGGATGACACGGGAAGTGAAATCTGTGCCGAGTTACTGAAAACTTTGTGACGCAGTAAATGAAAAAACCGCCTT
CGGGCGGTTTTTTTGTATGT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 841; Mature: 840

Protein sequence:

>841_residues
MTDQQKPQQENSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGYCTEIQVTIHSDNSISIV
DNGRGIPPAVKFDDKHEPKRSAAEIAMTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTVRRDGQVNLIEFAKG
EVQNRVIETVTGPDGQPVEVSPMKVIGATDKRGTEVHFLADEEIFTNVEFHYEVLSKRIRELSFLNNGVHIKLVDQRTGK
EEDFAFSGGVKGFVEYINRSKTTLHPNIFYANAEKDGISVEVAMQWNDGYNEQVLCFTNNIPQRDGGTHLTGLRAAMTRV
INKYIEENEVAKKAKVETSGDDMREGLACVLSVKVPEPKFSSQTKDKLVSSEVRLPVEELVGKALTDFLLETPNDAKIIC
GKIVDAARAREAARKAREMTRRKGVMDGMGLPGKLADCQEKDPAMSELFLVEGDSAGGSAKQGRDRKFQAILPLKGKILN
VERARFDKMLSSQEVLTLITALGTGIGKEDYNLEKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPDIIERGYVYIAQP
PLYKIKHGKEERYIKDDNELNAYMLKLAMEKASLSRPDGSEINGEALTELARQYQLAEGVIGRLSRIVDVDALRAIADGV
ALDLDNAAAAEASAVALKAKLAEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHVDADFVHGADY
AALARAATTFQGLIPDGTKVRRGEGEKMREQTVADFHQAMQWLLSEAERGVSRQRYKGLGEMNPEQLFETTLDVTQRRLL
RVQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVARNIDV

Sequences:

>Translated_841_residues
MTDQQKPQQENSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGYCTEIQVTIHSDNSISIV
DNGRGIPPAVKFDDKHEPKRSAAEIAMTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTVRRDGQVNLIEFAKG
EVQNRVIETVTGPDGQPVEVSPMKVIGATDKRGTEVHFLADEEIFTNVEFHYEVLSKRIRELSFLNNGVHIKLVDQRTGK
EEDFAFSGGVKGFVEYINRSKTTLHPNIFYANAEKDGISVEVAMQWNDGYNEQVLCFTNNIPQRDGGTHLTGLRAAMTRV
INKYIEENEVAKKAKVETSGDDMREGLACVLSVKVPEPKFSSQTKDKLVSSEVRLPVEELVGKALTDFLLETPNDAKIIC
GKIVDAARAREAARKAREMTRRKGVMDGMGLPGKLADCQEKDPAMSELFLVEGDSAGGSAKQGRDRKFQAILPLKGKILN
VERARFDKMLSSQEVLTLITALGTGIGKEDYNLEKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPDIIERGYVYIAQP
PLYKIKHGKEERYIKDDNELNAYMLKLAMEKASLSRPDGSEINGEALTELARQYQLAEGVIGRLSRIVDVDALRAIADGV
ALDLDNAAAAEASAVALKAKLAEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHVDADFVHGADY
AALARAATTFQGLIPDGTKVRRGEGEKMREQTVADFHQAMQWLLSEAERGVSRQRYKGLGEMNPEQLFETTLDVTQRRLL
RVQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVARNIDV
>Mature_840_residues
TDQQKPQQENSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGYCTEIQVTIHSDNSISIVD
NGRGIPPAVKFDDKHEPKRSAAEIAMTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTVRRDGQVNLIEFAKGE
VQNRVIETVTGPDGQPVEVSPMKVIGATDKRGTEVHFLADEEIFTNVEFHYEVLSKRIRELSFLNNGVHIKLVDQRTGKE
EDFAFSGGVKGFVEYINRSKTTLHPNIFYANAEKDGISVEVAMQWNDGYNEQVLCFTNNIPQRDGGTHLTGLRAAMTRVI
NKYIEENEVAKKAKVETSGDDMREGLACVLSVKVPEPKFSSQTKDKLVSSEVRLPVEELVGKALTDFLLETPNDAKIICG
KIVDAARAREAARKAREMTRRKGVMDGMGLPGKLADCQEKDPAMSELFLVEGDSAGGSAKQGRDRKFQAILPLKGKILNV
ERARFDKMLSSQEVLTLITALGTGIGKEDYNLEKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPDIIERGYVYIAQPP
LYKIKHGKEERYIKDDNELNAYMLKLAMEKASLSRPDGSEINGEALTELARQYQLAEGVIGRLSRIVDVDALRAIADGVA
LDLDNAAAAEASAVALKAKLAEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHVDADFVHGADYA
ALARAATTFQGLIPDGTKVRRGEGEKMREQTVADFHQAMQWLLSEAERGVSRQRYKGLGEMNPEQLFETTLDVTQRRLLR
VQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVARNIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=598, Percent_Identity=24.247491638796, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI19913406, Length=554, Percent_Identity=24.5487364620939, Blast_Score=101, Evalue=3e-21,
Organism=Escherichia coli, GI48994957, Length=833, Percent_Identity=58.2232893157263, Blast_Score=937, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=584, Percent_Identity=38.8698630136986, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17535065, Length=603, Percent_Identity=25.2072968490879, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=533, Percent_Identity=22.8893058161351, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI212645657, Length=154, Percent_Identity=35.7142857142857, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=612, Percent_Identity=23.0392156862745, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17136538, Length=558, Percent_Identity=24.0143369175627, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 93229; Mature: 93098

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQQKPQQENSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEAL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
AGYCTEIQVTIHSDNSISIVDNGRGIPPAVKFDDKHEPKRSAAEIAMTELHAGGKFNQNS
HCCEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
YKVSGGLHGVGVSCVNALSKWLRLTVRRDGQVNLIEFAKGEVQNRVIETVTGPDGQPVEV
EEECCCCCCHHHHHHHHHHHHHEEHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEE
SPMKVIGATDKRGTEVHFLADEEIFTNVEFHYEVLSKRIRELSFLNNGVHIKLVDQRTGK
CCEEEEECCCCCCCEEEEEECCHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
EEDFAFSGGVKGFVEYINRSKTTLHPNIFYANAEKDGISVEVAMQWNDGYNEQVLCFTNN
CCCCEECCCHHHHHHHHCCCCCEECCEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECC
IPQRDGGTHLTGLRAAMTRVINKYIEENEVAKKAKVETSGDDMREGLACVLSVKVPEPKF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEEEECCCCCC
SSQTKDKLVSSEVRLPVEELVGKALTDFLLETPNDAKIICGKIVDAARAREAARKAREMT
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
RRKGVMDGMGLPGKLADCQEKDPAMSELFLVEGDSAGGSAKQGRDRKFQAILPLKGKILN
HHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEC
VERARFDKMLSSQEVLTLITALGTGIGKEDYNLEKLRYHRIIIMTDADVDGSHIRTLLLT
HHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHH
FFYRQMPDIIERGYVYIAQPPLYKIKHGKEERYIKDDNELNAYMLKLAMEKASLSRPDGS
HHHHHHHHHHHCCEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
EINGEALTELARQYQLAEGVIGRLSRIVDVDALRAIADGVALDLDNAAAAEASAVALKAK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHH
LAEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHVDADFVHGADY
HHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCEEEEEEECCCCEEEEECCCHHHCCCCH
AALARAATTFQGLIPDGTKVRRGEGEKMREQTVADFHQAMQWLLSEAERGVSRQRYKGLG
HHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
EMNPEQLFETTLDVTQRRLLRVQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVARNID
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECCC
V
C
>Mature Secondary Structure 
TDQQKPQQENSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEAL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
AGYCTEIQVTIHSDNSISIVDNGRGIPPAVKFDDKHEPKRSAAEIAMTELHAGGKFNQNS
HCCEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
YKVSGGLHGVGVSCVNALSKWLRLTVRRDGQVNLIEFAKGEVQNRVIETVTGPDGQPVEV
EEECCCCCCHHHHHHHHHHHHHEEHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEE
SPMKVIGATDKRGTEVHFLADEEIFTNVEFHYEVLSKRIRELSFLNNGVHIKLVDQRTGK
CCEEEEECCCCCCCEEEEEECCHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
EEDFAFSGGVKGFVEYINRSKTTLHPNIFYANAEKDGISVEVAMQWNDGYNEQVLCFTNN
CCCCEECCCHHHHHHHHCCCCCEECCEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECC
IPQRDGGTHLTGLRAAMTRVINKYIEENEVAKKAKVETSGDDMREGLACVLSVKVPEPKF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEEEECCCCCC
SSQTKDKLVSSEVRLPVEELVGKALTDFLLETPNDAKIICGKIVDAARAREAARKAREMT
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
RRKGVMDGMGLPGKLADCQEKDPAMSELFLVEGDSAGGSAKQGRDRKFQAILPLKGKILN
HHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEC
VERARFDKMLSSQEVLTLITALGTGIGKEDYNLEKLRYHRIIIMTDADVDGSHIRTLLLT
HHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHH
FFYRQMPDIIERGYVYIAQPPLYKIKHGKEERYIKDDNELNAYMLKLAMEKASLSRPDGS
HHHHHHHHHHHCCEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
EINGEALTELARQYQLAEGVIGRLSRIVDVDALRAIADGVALDLDNAAAAEASAVALKAK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHH
LAEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHVDADFVHGADY
HHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCEEEEEEECCCCEEEEECCCHHHCCCCH
AALARAATTFQGLIPDGTKVRRGEGEKMREQTVADFHQAMQWLLSEAERGVSRQRYKGLG
HHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
EMNPEQLFETTLDVTQRRLLRVQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVARNID
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1906260 [H]