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The map label for this gene is nqrB [H]

Identifier: 93007249

GI number: 93007249

Start: 2998539

End: 2999774

Strand: Direct

Name: nqrB [H]

Synonym: Pcryo_2425

Alternate gene names: 93007249

Gene position: 2998539-2999774 (Clockwise)

Preceding gene: 93007248

Following gene: 93007250

Centisome position: 98.0

GC content: 46.04

Gene sequence:

>1236_bases
ATGAAATTTTTACACAATATGTTCGATCGTATGGAGCCGTCTTTCACCAAGGGTGGCAAACACACAAAATACTACGCCAT
CTTTGAGATGTTTGATACGTTTTTGCGTCAACCAAGCTCAACGACATACGCCTCATCACATGTACGCGACGGTATTGACC
TTAAGCGTATTATGATTACCGTCTGGTTATGTACCTTCCCAGCCATGTTTTGGGGTATGTACAATATCGGTCATCAAGCA
TTAACTGCGATTGCAACCCTTGGCTTACAGCCTGAAGGCTGGCGCACCATTATCACCAGTATGGCAGGCTACAACCCAGA
CAGTATCTGGGCAAGCTTCGTCTACGGTGCTATGCAGTTCTTACCGATTTATCTTGTGACCTTTGGGGTTGGTATTCTCT
GTGAGATTATCTTTGCCGTCGTCCGTGGTCATGAAGTCAACGAAGGCTTCTTTGTTACCTCTGTACTGTTTGCACTATGT
CTGCCACCCGATATCCCATTATGGCAAGTTGCCTTAGGTATTATCTTTGGTGTGGTCGTGGCAAAAGAGGTATTCGGTGG
TACAGGTAAAAACTTCCTTAACCCTGCCCTATCGGGTCGTGCGTTCTTATACTTTGCTTATCCGGCTTATATGTCAGGCG
ACTCAGTATGGACCGCGGTTGATGGTTTTTCAGGTGCAACGCCACTTGGTCTTGCTGCCCTTGGGGTTGTCCCACAAGAT
TTTGTCGATGTTTATGGCAATGCCATCACGTGGAGTGATGCATTCTTAGGCAACATGCAAGGCAGTATCGGTGAAGTATC
GACGCTAGCTATCTTAATGGGTGCTGCTGTCCTACTTTGGACGCGCATTGCCTCATGGCGCATCATGGCAGGTTGTGTGG
TTGGTCTGATTGCCACATCTCTTGTGTTTAATATGATTGGCTCTGAAGACAATATGATGATGAGCTTGCCGTTTTACTGG
CACTTAGTGATCGGTGGTTTTGCTTTTGGTGCCGTGTTTATGGCAACTGATCCAGTATCTGCTGCGCATACCAATAAAGG
GCGCTGGGCTTACGGTATCTTGATTGGTTTTATGACCGTATTGATTCGTGTCATTAATCCAGCTTTCCCAGAAGGCATCA
TGCTAGCCATTTTATTTGCTAACTTATTTGCGCCACTGTTTGACTATTTTGTCACTCAAGCAAATATCAAACGCCAAACT
GCTCGGAGGGTTCGCTATGTCCAAGCCCAAAAGTAA

Upstream 100 bases:

>100_bases
AAGATTTAGCATTATGCACCTTCGTATCTCCTGGCAAATATGAATTCGGCGATATTTTGCGTGATAACTTAACGCGCATT
GAGCTGGAGGGCTAACCACG

Downstream 100 bases:

>100_bases
TAACGCAAAAACCATCAGTGTGGCGTTGACGCTATGCTTGGTGTGTTCGGTATTGGTCTCAGCTGCGGCTGTGGGTCTAA
AGCCTGCGCAAGTAGAAAAC

Product: Na(+)-translocating NADH-quinone reductase subunit B

Products: NA

Alternate protein names: Na(+)-NQR subunit B; Na(+)-translocating NQR subunit B; NQR complex subunit B; NQR-1 subunit B [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKFLHNMFDRMEPSFTKGGKHTKYYAIFEMFDTFLRQPSSTTYASSHVRDGIDLKRIMITVWLCTFPAMFWGMYNIGHQA
LTAIATLGLQPEGWRTIITSMAGYNPDSIWASFVYGAMQFLPIYLVTFGVGILCEIIFAVVRGHEVNEGFFVTSVLFALC
LPPDIPLWQVALGIIFGVVVAKEVFGGTGKNFLNPALSGRAFLYFAYPAYMSGDSVWTAVDGFSGATPLGLAALGVVPQD
FVDVYGNAITWSDAFLGNMQGSIGEVSTLAILMGAAVLLWTRIASWRIMAGCVVGLIATSLVFNMIGSEDNMMMSLPFYW
HLVIGGFAFGAVFMATDPVSAAHTNKGRWAYGILIGFMTVLIRVINPAFPEGIMLAILFANLFAPLFDYFVTQANIKRQT
ARRVRYVQAQK

Sequences:

>Translated_411_residues
MKFLHNMFDRMEPSFTKGGKHTKYYAIFEMFDTFLRQPSSTTYASSHVRDGIDLKRIMITVWLCTFPAMFWGMYNIGHQA
LTAIATLGLQPEGWRTIITSMAGYNPDSIWASFVYGAMQFLPIYLVTFGVGILCEIIFAVVRGHEVNEGFFVTSVLFALC
LPPDIPLWQVALGIIFGVVVAKEVFGGTGKNFLNPALSGRAFLYFAYPAYMSGDSVWTAVDGFSGATPLGLAALGVVPQD
FVDVYGNAITWSDAFLGNMQGSIGEVSTLAILMGAAVLLWTRIASWRIMAGCVVGLIATSLVFNMIGSEDNMMMSLPFYW
HLVIGGFAFGAVFMATDPVSAAHTNKGRWAYGILIGFMTVLIRVINPAFPEGIMLAILFANLFAPLFDYFVTQANIKRQT
ARRVRYVQAQK
>Mature_411_residues
MKFLHNMFDRMEPSFTKGGKHTKYYAIFEMFDTFLRQPSSTTYASSHVRDGIDLKRIMITVWLCTFPAMFWGMYNIGHQA
LTAIATLGLQPEGWRTIITSMAGYNPDSIWASFVYGAMQFLPIYLVTFGVGILCEIIFAVVRGHEVNEGFFVTSVLFALC
LPPDIPLWQVALGIIFGVVVAKEVFGGTGKNFLNPALSGRAFLYFAYPAYMSGDSVWTAVDGFSGATPLGLAALGVVPQD
FVDVYGNAITWSDAFLGNMQGSIGEVSTLAILMGAAVLLWTRIASWRIMAGCVVGLIATSLVFNMIGSEDNMMMSLPFYW
HLVIGGFAFGAVFMATDPVSAAHTNKGRWAYGILIGFMTVLIRVINPAFPEGIMLAILFANLFAPLFDYFVTQANIKRQT
ARRVRYVQAQK

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. NqrA to nqrE are probably involved in the second step, the conversion of ubisemiqui

COG id: COG1805

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrB/rnfD family [H]

Homologues:

Organism=Escherichia coli, GI1787917, Length=296, Percent_Identity=28.7162162162162, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010966
- InterPro:   IPR004338 [H]

Pfam domain/function: PF03116 NQR2_RnfD_RnfE [H]

EC number: NA

Molecular weight: Translated: 45316; Mature: 45316

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLHNMFDRMEPSFTKGGKHTKYYAIFEMFDTFLRQPSSTTYASSHVRDGIDLKRIMIT
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHH
VWLCTFPAMFWGMYNIGHQALTAIATLGLQPEGWRTIITSMAGYNPDSIWASFVYGAMQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LPIYLVTFGVGILCEIIFAVVRGHEVNEGFFVTSVLFALCLPPDIPLWQVALGIIFGVVV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AKEVFGGTGKNFLNPALSGRAFLYFAYPAYMSGDSVWTAVDGFSGATPLGLAALGVVPQD
HHHHHCCCCCHHHCCCCCCCEEEEEECHHHCCCCCEEEEECCCCCCCCHHHHHHCCCCHH
FVDVYGNAITWSDAFLGNMQGSIGEVSTLAILMGAAVLLWTRIASWRIMAGCVVGLIATS
HHHHCCCCEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVFNMIGSEDNMMMSLPFYWHLVIGGFAFGAVFMATDPVSAAHTNKGRWAYGILIGFMTV
HHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
LIRVINPAFPEGIMLAILFANLFAPLFDYFVTQANIKRQTARRVRYVQAQK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFLHNMFDRMEPSFTKGGKHTKYYAIFEMFDTFLRQPSSTTYASSHVRDGIDLKRIMIT
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHH
VWLCTFPAMFWGMYNIGHQALTAIATLGLQPEGWRTIITSMAGYNPDSIWASFVYGAMQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LPIYLVTFGVGILCEIIFAVVRGHEVNEGFFVTSVLFALCLPPDIPLWQVALGIIFGVVV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AKEVFGGTGKNFLNPALSGRAFLYFAYPAYMSGDSVWTAVDGFSGATPLGLAALGVVPQD
HHHHHCCCCCHHHCCCCCCCEEEEEECHHHCCCCCEEEEECCCCCCCCHHHHHHCCCCHH
FVDVYGNAITWSDAFLGNMQGSIGEVSTLAILMGAAVLLWTRIASWRIMAGCVVGLIATS
HHHHCCCCEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVFNMIGSEDNMMMSLPFYWHLVIGGFAFGAVFMATDPVSAAHTNKGRWAYGILIGFMTV
HHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
LIRVINPAFPEGIMLAILFANLFAPLFDYFVTQANIKRQTARRVRYVQAQK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA