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The map label for this gene is murF [H]

Identifier: 93007200

GI number: 93007200

Start: 2920667

End: 2922115

Strand: Reverse

Name: murF [H]

Synonym: Pcryo_2376

Alternate gene names: 93007200

Gene position: 2922115-2920667 (Counterclockwise)

Preceding gene: 93007201

Following gene: 93007199

Centisome position: 95.5

GC content: 47.41

Gene sequence:

>1449_bases
ATGACAGCCGACAACGATAACAGCATTCAGTCGCAAGGGCTGTATGTTTGGCAAGAAGACAATCTGCTCGCAGCAACCGC
AGCACTCGACGGGCATTGGCAGCTGAGTGAGGAACAATCCGGCTCAAAAAACGATGTAATGAGCACTCGTATTGCCACCG
ACACCCGTACGATAAAGCCTGGTGATATATTTTTAGCGTTAACGGGTGACAATTTTGATGGTCACGATTATCTCGATACT
GCCATTGCACAAGGTGCGGTTGCGGCTATCGTCGCCCGCCCTATTGCTACAGCTGATGCAAATAGTATTCCGCAGTTAGT
GGTGAGCGATACCCGTTTAGCGTTAGGGCAATTGGCTGCGTATCGTCGTCAGCAGCATCAAAATTTAACCGTCATTGCTA
TTACTGGCTCTAGTGGTAAGACCACCTGTAAAGAGATGCTCGGTAGTATCTTTGGCAGACTTGCCCCAACGCTTATCACG
CGTGGCAACCTAAATAATGATTTGGGTGTGCCGATGATGTTGCTCGAATTATCCGACCATCATCGCTATGCCATCCTAGA
GCTTGGCGCAAACCATATTGGCGAAATTGCTTATACTACGGAGATTGTCCAGCCAGATGTTGCGTGTATTTTAAATATTG
GCACGGCGCATTTGGGTGAGTTTGGCAGCCGTGAAGGGATTTGCCAAACCAAGGCTGAGATTTACCATACCTTGAATGAC
AGCCAATTTGCGATCGTTCCTGATAAAGATGATTTTACCAATCAATTACGCCGTATCGCTGAAAAGCATACATCACACGT
GATTGGCTTTGGTAGTACTGATGTCAGTGCCAGCCATTTAGATGTCGAGCCTGAGCGCAGTGAATTTAAGCTGCATATAG
GCAATCAAGTCCATGATATCAACTTGCCACTAGCGGGCGAGCACAATGTCAATAATGCTTTAGCCGCTGCTGCCTGTGCC
CATGCATTAAATATTGAAGTTAGTGATATCGTCGTTGGACTTGAAAATGCGCGTCCTGCCAAAGGTCGCTTGAACAGTCA
GCTGTTAGGCATGCATCGACTGATTGATGATACTTATAATGCCAATCCACATTCGGTGCGAGCTGCAGCAAAAGTATTGG
CAGCACAAACGGGCACGCAAGTCATGGTGCTGGGCGATATCGCAGAACTGGGAGAGGCGGCGGTTAGCGAGCATCAAAGC
TTGGGTCGTACCATTGCGACCACAGGTATTAATGTACTGCTTTGCGTTGGTGAATATGCCCCTTATACAGTCGAAGGTGC
GCAGGAGATTTCTGATATCAATGCCTATGCGTTTACCGATAAAGACAGTTTATTGGCGTACTTGAAACCTTATTTGCAAG
CGCAGCAGGCGCAACCTTGTACGGTGTTGTTTAAAGGTTCTCGTTCCATGGAAATGGAAACACTTATCAATGCGCTAGTC
GAGGAGTAG

Upstream 100 bases:

>100_bases
GCGCTTACAGTTAGTTTTTCATGCTATCTAAAAAGTATTAGTCGTTAACTATAAAAAATAAATAAATAAGTAAGTAGTCA
TCATATATAAGGTAATCATT

Downstream 100 bases:

>100_bases
GCAGTGTTAGTTTGGTTGTTTAGCTGGCTTGGTCATTATTATGCCCCGTTTTATGCGGTTTCTTCTTTGACGCTGCGCGC
GTTACTGGCGGTCGTTACCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTADNDNSIQSQGLYVWQEDNLLAATAALDGHWQLSEEQSGSKNDVMSTRIATDTRTIKPGDIFLALTGDNFDGHDYLDT
AIAQGAVAAIVARPIATADANSIPQLVVSDTRLALGQLAAYRRQQHQNLTVIAITGSSGKTTCKEMLGSIFGRLAPTLIT
RGNLNNDLGVPMMLLELSDHHRYAILELGANHIGEIAYTTEIVQPDVACILNIGTAHLGEFGSREGICQTKAEIYHTLND
SQFAIVPDKDDFTNQLRRIAEKHTSHVIGFGSTDVSASHLDVEPERSEFKLHIGNQVHDINLPLAGEHNVNNALAAAACA
HALNIEVSDIVVGLENARPAKGRLNSQLLGMHRLIDDTYNANPHSVRAAAKVLAAQTGTQVMVLGDIAELGEAAVSEHQS
LGRTIATTGINVLLCVGEYAPYTVEGAQEISDINAYAFTDKDSLLAYLKPYLQAQQAQPCTVLFKGSRSMEMETLINALV
EE

Sequences:

>Translated_482_residues
MTADNDNSIQSQGLYVWQEDNLLAATAALDGHWQLSEEQSGSKNDVMSTRIATDTRTIKPGDIFLALTGDNFDGHDYLDT
AIAQGAVAAIVARPIATADANSIPQLVVSDTRLALGQLAAYRRQQHQNLTVIAITGSSGKTTCKEMLGSIFGRLAPTLIT
RGNLNNDLGVPMMLLELSDHHRYAILELGANHIGEIAYTTEIVQPDVACILNIGTAHLGEFGSREGICQTKAEIYHTLND
SQFAIVPDKDDFTNQLRRIAEKHTSHVIGFGSTDVSASHLDVEPERSEFKLHIGNQVHDINLPLAGEHNVNNALAAAACA
HALNIEVSDIVVGLENARPAKGRLNSQLLGMHRLIDDTYNANPHSVRAAAKVLAAQTGTQVMVLGDIAELGEAAVSEHQS
LGRTIATTGINVLLCVGEYAPYTVEGAQEISDINAYAFTDKDSLLAYLKPYLQAQQAQPCTVLFKGSRSMEMETLINALV
EE
>Mature_481_residues
TADNDNSIQSQGLYVWQEDNLLAATAALDGHWQLSEEQSGSKNDVMSTRIATDTRTIKPGDIFLALTGDNFDGHDYLDTA
IAQGAVAAIVARPIATADANSIPQLVVSDTRLALGQLAAYRRQQHQNLTVIAITGSSGKTTCKEMLGSIFGRLAPTLITR
GNLNNDLGVPMMLLELSDHHRYAILELGANHIGEIAYTTEIVQPDVACILNIGTAHLGEFGSREGICQTKAEIYHTLNDS
QFAIVPDKDDFTNQLRRIAEKHTSHVIGFGSTDVSASHLDVEPERSEFKLHIGNQVHDINLPLAGEHNVNNALAAAACAH
ALNIEVSDIVVGLENARPAKGRLNSQLLGMHRLIDDTYNANPHSVRAAAKVLAAQTGTQVMVLGDIAELGEAAVSEHQSL
GRTIATTGINVLLCVGEYAPYTVEGAQEISDINAYAFTDKDSLLAYLKPYLQAQQAQPCTVLFKGSRSMEMETLINALVE
E

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=442, Percent_Identity=41.6289592760181, Blast_Score=282, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51923; Mature: 51792

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADNDNSIQSQGLYVWQEDNLLAATAALDGHWQLSEEQSGSKNDVMSTRIATDTRTIKP
CCCCCCCCCCCCCEEEEECCCEEEEEEEECCCEEECCCCCCCCCCHHEEEEECCCCEECC
GDIFLALTGDNFDGHDYLDTAIAQGAVAAIVARPIATADANSIPQLVVSDTRLALGQLAA
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHH
YRRQQHQNLTVIAITGSSGKTTCKEMLGSIFGRLAPTLITRGNLNNDLGVPMMLLELSDH
HHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEECCC
HRYAILELGANHIGEIAYTTEIVQPDVACILNIGTAHLGEFGSREGICQTKAEIYHTLND
CCEEEEEECCCHHCCHHEEHHHCCCCEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHCCC
SQFAIVPDKDDFTNQLRRIAEKHTSHVIGFGSTDVSASHLDVEPERSEFKLHIGNQVHDI
CEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCEECCCCCCCCEEEEECCEEEEE
NLPLAGEHNVNNALAAAACAHALNIEVSDIVVGLENARPAKGRLNSQLLGMHRLIDDTYN
CCCCCCCCCCCHHHHHHHHHHHCCCEEHHEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
ANPHSVRAAAKVLAAQTGTQVMVLGDIAELGEAAVSEHQSLGRTIATTGINVLLCVGEYA
CCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCEEHHCCCEEEEEECCCC
PYTVEGAQEISDINAYAFTDKDSLLAYLKPYLQAQQAQPCTVLFKGSRSMEMETLINALV
CEEECCHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
EE
CC
>Mature Secondary Structure 
TADNDNSIQSQGLYVWQEDNLLAATAALDGHWQLSEEQSGSKNDVMSTRIATDTRTIKP
CCCCCCCCCCCCEEEEECCCEEEEEEEECCCEEECCCCCCCCCCHHEEEEECCCCEECC
GDIFLALTGDNFDGHDYLDTAIAQGAVAAIVARPIATADANSIPQLVVSDTRLALGQLAA
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHH
YRRQQHQNLTVIAITGSSGKTTCKEMLGSIFGRLAPTLITRGNLNNDLGVPMMLLELSDH
HHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEECCC
HRYAILELGANHIGEIAYTTEIVQPDVACILNIGTAHLGEFGSREGICQTKAEIYHTLND
CCEEEEEECCCHHCCHHEEHHHCCCCEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHCCC
SQFAIVPDKDDFTNQLRRIAEKHTSHVIGFGSTDVSASHLDVEPERSEFKLHIGNQVHDI
CEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCEECCCCCCCCEEEEECCEEEEE
NLPLAGEHNVNNALAAAACAHALNIEVSDIVVGLENARPAKGRLNSQLLGMHRLIDDTYN
CCCCCCCCCCCHHHHHHHHHHHCCCEEHHEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
ANPHSVRAAAKVLAAQTGTQVMVLGDIAELGEAAVSEHQSLGRTIATTGINVLLCVGEYA
CCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCEEHHCCCEEEEEECCCC
PYTVEGAQEISDINAYAFTDKDSLLAYLKPYLQAQQAQPCTVLFKGSRSMEMETLINALV
CEEECCHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]