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The map label for this gene is murE [H]

Identifier: 93007201

GI number: 93007201

Start: 2922235

End: 2923947

Strand: Reverse

Name: murE [H]

Synonym: Pcryo_2377

Alternate gene names: 93007201

Gene position: 2923947-2922235 (Counterclockwise)

Preceding gene: 93007202

Following gene: 93007200

Centisome position: 95.56

GC content: 47.93

Gene sequence:

>1713_bases
ATGACCCTGTCTACCTCGTCGCCAAGCAGTATCACAGTTACCTTGCAACAGCTGCTTGCAGCTAGTAGCAGCGACGATAG
ACATAGTCTACAGCAGGTATTTACAGCGAATATGACCGCAGCTGTTAGCGAGTCATCGCATCAAATAACCGCTGTTCAAC
TGGATGCGGTGATCAATATTCCATTCCAGCAGTTCTGTTTAGACAGCCGTCAACTAGCCAGCAACGACGTGTTCGTCTTG
TTAAAAAGCCATACGCCAAATTGCCAAAAAAGCCGTGACCACCTTTATCAAGCCGCTAACAATGCGGCTTTTATCCTATC
AGAAATAGATCCTAGGGCTTTACTTAACACGACTAGTGTATCAAATCATGCCGATATCACCTTACCAAATGATAGTACTG
CTAAAGCGCAGCAGCAATTAGTAGCACTGCCTTGCCCCGTTTTACATGTGCCCAACATTCGTGATTTTTTAGGGACACTC
ATTCAAGCACGTTTGCAGTATCAGCAGCCCGTGACATTACCAACTGTCATAGCCGTGACGGGCACCAATGGCAAAACCAC
CATTAGCCAATTGGTGGCACAATTGACGCAGCTGACAGGTATGAGCAGCGCAGTCATGGGCACGGCAGGTAATGGTAGGT
TTGGTGCTTTGGTGCAAGCCAGCCATACCACGGGAGATGTTTTAGCGGTCCAGCAGTTTTTGTATCAAATGGGCAAAGAA
GGTGCTGAACTACTAGCATTGGAAGCCAGCTCACATGGTTTAGATCAGCAGCGTCTGCAAGGTATGCCAGTGTCGGTGGC
GATTTATACCAACCTGAGTCGCGATCATTTAGATTATCATGCTGATATGGCAGAGTACGCGGCAGCTAAAGCCAAGTTGT
TTGATAAAGCCCATTTTCCGGATTTGACCCATGCCATTATCAATATCGATGACGAGCATGCCCAGATTATGCTTGACACC
GCACACGCTAGCAATTTGACCGTTTGGACATACAGCTTAGATTCACTAAAAACAGCTACCTTCGTCGCCGCTAACATCAC
GCCAAGTTTACGAGGTGTAGAGATCAGCCTGCGTACAGATTTCGACGATGGGACAATGAATCATTTAGATATCGTTAGCC
CATTATTGGGACGTTTTAACGTGGCGAATTTACTTGCTGCTATGGCAGCTGCTGTAGCTTTAGGCATTAGCCTTGAGCAT
ATTGCCGCAGTGGTGCCGCAGTTACAAGGTGCGGTTGGGCGTATGCAGCGTGTACCATCGAATGATGGTTGTTTCATCGT
CGATTATGCTCACACCCCTGATGCCTTAAGTCAGGTGCTTGCCAGTCTCAAAACTCATTGTAAAGGCCAGCTATGGGCAG
TGTTTGGCTGCGGTGGCGACCGTGATGCGGGCAAACGTCCTTTGATGGCGCAAGCAGGATTGGCAGGCGCGGATAAAGTC
GTGTTAACGGCAGACAATCCACGTACTGAAGACCCCAACGCGATTTTGCAAGATATGCAGGTAGGGATGAGCACTGAGCA
ATATCAGCGTACCCATATTGAGCCTGCGCGCCAAGCAGCGATTGAGTATGCGGTCAATCAAGCAGCGGCTGATGATATCG
TCGTTATTGCTGGCAAGGGTCATGAGACCTATCAAGAGATTAATCATGTTCGTTATGATTTTGACGACAGTGTTATTTTG
CAAAATGCCTTAAAACAAGCAGGGCGCGTATAG

Upstream 100 bases:

>100_bases
GATAAGCCGCTCAAAACCGAAGCTCAATAGATATAAAAGTAAATTTTTTAATATCCACCTCTTAACTATTTGATGTGTTT
TAACCGTTTAGGATTTGCCA

Downstream 100 bases:

>100_bases
CATTTATAGATAAATAATAGCGCTTACAGTTAGTTTTTCATGCTATCTAAAAAGTATTAGTCGTTAACTATAAAAAATAA
ATAAATAAGTAAGTAGTCAT

Product: UDP-N-acetylmuramyl-tripeptide synthetases

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MTLSTSSPSSITVTLQQLLAASSSDDRHSLQQVFTANMTAAVSESSHQITAVQLDAVINIPFQQFCLDSRQLASNDVFVL
LKSHTPNCQKSRDHLYQAANNAAFILSEIDPRALLNTTSVSNHADITLPNDSTAKAQQQLVALPCPVLHVPNIRDFLGTL
IQARLQYQQPVTLPTVIAVTGTNGKTTISQLVAQLTQLTGMSSAVMGTAGNGRFGALVQASHTTGDVLAVQQFLYQMGKE
GAELLALEASSHGLDQQRLQGMPVSVAIYTNLSRDHLDYHADMAEYAAAKAKLFDKAHFPDLTHAIINIDDEHAQIMLDT
AHASNLTVWTYSLDSLKTATFVAANITPSLRGVEISLRTDFDDGTMNHLDIVSPLLGRFNVANLLAAMAAAVALGISLEH
IAAVVPQLQGAVGRMQRVPSNDGCFIVDYAHTPDALSQVLASLKTHCKGQLWAVFGCGGDRDAGKRPLMAQAGLAGADKV
VLTADNPRTEDPNAILQDMQVGMSTEQYQRTHIEPARQAAIEYAVNQAAADDIVVIAGKGHETYQEINHVRYDFDDSVIL
QNALKQAGRV

Sequences:

>Translated_570_residues
MTLSTSSPSSITVTLQQLLAASSSDDRHSLQQVFTANMTAAVSESSHQITAVQLDAVINIPFQQFCLDSRQLASNDVFVL
LKSHTPNCQKSRDHLYQAANNAAFILSEIDPRALLNTTSVSNHADITLPNDSTAKAQQQLVALPCPVLHVPNIRDFLGTL
IQARLQYQQPVTLPTVIAVTGTNGKTTISQLVAQLTQLTGMSSAVMGTAGNGRFGALVQASHTTGDVLAVQQFLYQMGKE
GAELLALEASSHGLDQQRLQGMPVSVAIYTNLSRDHLDYHADMAEYAAAKAKLFDKAHFPDLTHAIINIDDEHAQIMLDT
AHASNLTVWTYSLDSLKTATFVAANITPSLRGVEISLRTDFDDGTMNHLDIVSPLLGRFNVANLLAAMAAAVALGISLEH
IAAVVPQLQGAVGRMQRVPSNDGCFIVDYAHTPDALSQVLASLKTHCKGQLWAVFGCGGDRDAGKRPLMAQAGLAGADKV
VLTADNPRTEDPNAILQDMQVGMSTEQYQRTHIEPARQAAIEYAVNQAAADDIVVIAGKGHETYQEINHVRYDFDDSVIL
QNALKQAGRV
>Mature_569_residues
TLSTSSPSSITVTLQQLLAASSSDDRHSLQQVFTANMTAAVSESSHQITAVQLDAVINIPFQQFCLDSRQLASNDVFVLL
KSHTPNCQKSRDHLYQAANNAAFILSEIDPRALLNTTSVSNHADITLPNDSTAKAQQQLVALPCPVLHVPNIRDFLGTLI
QARLQYQQPVTLPTVIAVTGTNGKTTISQLVAQLTQLTGMSSAVMGTAGNGRFGALVQASHTTGDVLAVQQFLYQMGKEG
AELLALEASSHGLDQQRLQGMPVSVAIYTNLSRDHLDYHADMAEYAAAKAKLFDKAHFPDLTHAIINIDDEHAQIMLDTA
HASNLTVWTYSLDSLKTATFVAANITPSLRGVEISLRTDFDDGTMNHLDIVSPLLGRFNVANLLAAMAAAVALGISLEHI
AAVVPQLQGAVGRMQRVPSNDGCFIVDYAHTPDALSQVLASLKTHCKGQLWAVFGCGGDRDAGKRPLMAQAGLAGADKVV
LTADNPRTEDPNAILQDMQVGMSTEQYQRTHIEPARQAAIEYAVNQAAADDIVVIAGKGHETYQEINHVRYDFDDSVILQ
NALKQAGRV

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=506, Percent_Identity=37.5494071146245, Blast_Score=265, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 61301; Mature: 61170

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSTSSPSSITVTLQQLLAASSSDDRHSLQQVFTANMTAAVSESSHQITAVQLDAVINI
CCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHCHHHEECCCCCEEEEEEEEEEECC
PFQQFCLDSRQLASNDVFVLLKSHTPNCQKSRDHLYQAANNAAFILSEIDPRALLNTTSV
CHHHHHCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHEECCCC
SNHADITLPNDSTAKAQQQLVALPCPVLHVPNIRDFLGTLIQARLQYQQPVTLPTVIAVT
CCCCEEEECCCCHHHHHHHHEECCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEE
GTNGKTTISQLVAQLTQLTGMSSAVMGTAGNGRFGALVQASHTTGDVLAVQQFLYQMGKE
CCCCCHHHHHHHHHHHHHCCCCHHHEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCC
GAELLALEASSHGLDQQRLQGMPVSVAIYTNLSRDHLDYHADMAEYAAAKAKLFDKAHFP
CCEEEEEECCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLTHAIINIDDEHAQIMLDTAHASNLTVWTYSLDSLKTATFVAANITPSLRGVEISLRTD
CHHHHEEECCCCCEEEEEEECCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEEEEEEC
FDDGTMNHLDIVSPLLGRFNVANLLAAMAAAVALGISLEHIAAVVPQLQGAVGRMQRVPS
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
NDGCFIVDYAHTPDALSQVLASLKTHCKGQLWAVFGCGGDRDAGKRPLMAQAGLAGADKV
CCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCEE
VLTADNPRTEDPNAILQDMQVGMSTEQYQRTHIEPARQAAIEYAVNQAAADDIVVIAGKG
EEECCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCC
HETYQEINHVRYDFDDSVILQNALKQAGRV
CHHHHHHHHEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLSTSSPSSITVTLQQLLAASSSDDRHSLQQVFTANMTAAVSESSHQITAVQLDAVINI
CCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHCHHHEECCCCCEEEEEEEEEEECC
PFQQFCLDSRQLASNDVFVLLKSHTPNCQKSRDHLYQAANNAAFILSEIDPRALLNTTSV
CHHHHHCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHEECCCC
SNHADITLPNDSTAKAQQQLVALPCPVLHVPNIRDFLGTLIQARLQYQQPVTLPTVIAVT
CCCCEEEECCCCHHHHHHHHEECCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEE
GTNGKTTISQLVAQLTQLTGMSSAVMGTAGNGRFGALVQASHTTGDVLAVQQFLYQMGKE
CCCCCHHHHHHHHHHHHHCCCCHHHEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCC
GAELLALEASSHGLDQQRLQGMPVSVAIYTNLSRDHLDYHADMAEYAAAKAKLFDKAHFP
CCEEEEEECCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLTHAIINIDDEHAQIMLDTAHASNLTVWTYSLDSLKTATFVAANITPSLRGVEISLRTD
CHHHHEEECCCCCEEEEEEECCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEEEEEEC
FDDGTMNHLDIVSPLLGRFNVANLLAAMAAAVALGISLEHIAAVVPQLQGAVGRMQRVPS
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
NDGCFIVDYAHTPDALSQVLASLKTHCKGQLWAVFGCGGDRDAGKRPLMAQAGLAGADKV
CCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCEE
VLTADNPRTEDPNAILQDMQVGMSTEQYQRTHIEPARQAAIEYAVNQAAADDIVVIAGKG
EEECCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCC
HETYQEINHVRYDFDDSVILQNALKQAGRV
CHHHHHHHHEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]