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The map label for this gene is mdeA [H]

Identifier: 93007025

GI number: 93007025

Start: 2704801

End: 2706105

Strand: Reverse

Name: mdeA [H]

Synonym: Pcryo_2201

Alternate gene names: 93007025

Gene position: 2706105-2704801 (Counterclockwise)

Preceding gene: 93007026

Following gene: 93007022

Centisome position: 88.44

GC content: 45.9

Gene sequence:

>1305_bases
ATGAGTGACGGACAACCAGCTAAGCAAAATAATCAACGCCTTGAAACCTTGGCGATTCATGCCGGATATACTGTAGAGCC
GACCACCAAAGCGGTTGCTGTGCCCATTTATCATACCAGCTCATATGCCTTTGATAATACTCAGCATGGTGCAGACTTAT
TTGATTTAAAAGTTGCTGGCAACATTTATACCCGTATCATGAACCCTACCAATGCGGTACTAGAAGAGCGCGTTGCTGCA
CTTGAGGGCGGTATCGGCGCGCTTGCTGTGGCTTCTGGTATGGCTGCCATCACTTATGCGATTCAAACCATTTGTGAAGC
AGGCGACAATATCGTTGCGGTATCGACACTATATGGTGGTACTTATAATTTATTCGCTCATGCATTACCACGACAAGGTA
TAGAGGTGCGCTTTTTTGACCATAAAAATCCTGCCGCGATTCGTGATTTAATCGATGATAAGACTAAAATGGTCTTTGCG
GAAAGTATTGGTAATCCACTCGGCAATATTATTGATATCCAAGCACTCAGTGATATCGCGCATGAATATGGCGTGCCAGT
GGTGATTGATAGCACCGTTGCGACACCTGCATTATGTCGTCCATTTGAGTTTGGTGCGGATATTGTCATTCATTCATTGA
CCAAATATATGAGCGGTACCGGTACCTCGATTGGTGGCGCTATTGTCGATAGTGGTACTTTCCCTTGGGACGATTATCCT
GAACGTTTTCCGTTATTAAATACCCCAGATGCTAGCTATCATGGCGTAAACTTCGTCAAAGACGTTGGCGCTGCGGCATT
TATCGCCCGTGCTCGTGTGGCACCGCTGCGAAATACTGGCGCAGCGTTAAGCCCGCTCAATGCCTTTAGTATTTTGCAAG
GTTTACAGACGCTAGGTTTACGTATGGAGCGTCATGTTCAAAACGCGCAAGCGGTTGCAGAGTATCTACGTGATCATGAT
AAAGTTGCTTGGGTAAAATATGCTGGTCTGCCTGACCATCCTGAGCATGAACTTGCCAAAAAATACATGAAAGGTACGCC
ATCTGCTATTTTGACTTTTGGGGTAAAAGGTGGGCTTGAAGCAGGCGCGCGCTTTATTGATGCACTGCAACTGATTACAC
GCTTGGTGAATATCGGTGATGCCAAATCATTAGCTTGTCATCCAGCAACGACCACCCATCGCCAGCTCAATGAGCAAGAG
CTCGAAAAGGCAGGCGTCAGCATTGATATGGTTCGTTTATCAATTGGTATTGAGCATATCGAAGATATCAAAGCCGATTT
AGAGCAAGCATTGGCTGCGGCTTAA

Upstream 100 bases:

>100_bases
AGTTATGATTAACTCAGCTTTTTTTATGCCTATCGTTTAGCGATAGCTATTTTAATCACCAAATATTTTTTGATAGCGAA
TAAATATATAGGAGCCCAAT

Downstream 100 bases:

>100_bases
TTAAATAATAGCTTATAAAGCAGAAAGCCCATATCAAATCGATATGGGCTTTTTTATTTTCTAAGCTATCTAAATACTGC
TAATTATTAGGCTTAATTTT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSDGQPAKQNNQRLETLAIHAGYTVEPTTKAVAVPIYHTSSYAFDNTQHGADLFDLKVAGNIYTRIMNPTNAVLEERVAA
LEGGIGALAVASGMAAITYAIQTICEAGDNIVAVSTLYGGTYNLFAHALPRQGIEVRFFDHKNPAAIRDLIDDKTKMVFA
ESIGNPLGNIIDIQALSDIAHEYGVPVVIDSTVATPALCRPFEFGADIVIHSLTKYMSGTGTSIGGAIVDSGTFPWDDYP
ERFPLLNTPDASYHGVNFVKDVGAAAFIARARVAPLRNTGAALSPLNAFSILQGLQTLGLRMERHVQNAQAVAEYLRDHD
KVAWVKYAGLPDHPEHELAKKYMKGTPSAILTFGVKGGLEAGARFIDALQLITRLVNIGDAKSLACHPATTTHRQLNEQE
LEKAGVSIDMVRLSIGIEHIEDIKADLEQALAAA

Sequences:

>Translated_434_residues
MSDGQPAKQNNQRLETLAIHAGYTVEPTTKAVAVPIYHTSSYAFDNTQHGADLFDLKVAGNIYTRIMNPTNAVLEERVAA
LEGGIGALAVASGMAAITYAIQTICEAGDNIVAVSTLYGGTYNLFAHALPRQGIEVRFFDHKNPAAIRDLIDDKTKMVFA
ESIGNPLGNIIDIQALSDIAHEYGVPVVIDSTVATPALCRPFEFGADIVIHSLTKYMSGTGTSIGGAIVDSGTFPWDDYP
ERFPLLNTPDASYHGVNFVKDVGAAAFIARARVAPLRNTGAALSPLNAFSILQGLQTLGLRMERHVQNAQAVAEYLRDHD
KVAWVKYAGLPDHPEHELAKKYMKGTPSAILTFGVKGGLEAGARFIDALQLITRLVNIGDAKSLACHPATTTHRQLNEQE
LEKAGVSIDMVRLSIGIEHIEDIKADLEQALAAA
>Mature_433_residues
SDGQPAKQNNQRLETLAIHAGYTVEPTTKAVAVPIYHTSSYAFDNTQHGADLFDLKVAGNIYTRIMNPTNAVLEERVAAL
EGGIGALAVASGMAAITYAIQTICEAGDNIVAVSTLYGGTYNLFAHALPRQGIEVRFFDHKNPAAIRDLIDDKTKMVFAE
SIGNPLGNIIDIQALSDIAHEYGVPVVIDSTVATPALCRPFEFGADIVIHSLTKYMSGTGTSIGGAIVDSGTFPWDDYPE
RFPLLNTPDASYHGVNFVKDVGAAAFIARARVAPLRNTGAALSPLNAFSILQGLQTLGLRMERHVQNAQAVAEYLRDHDK
VAWVKYAGLPDHPEHELAKKYMKGTPSAILTFGVKGGLEAGARFIDALQLITRLVNIGDAKSLACHPATTTHRQLNEQEL
EKAGVSIDMVRLSIGIEHIEDIKADLEQALAAA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=426, Percent_Identity=34.2723004694836, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI299473759, Length=426, Percent_Identity=30.7511737089202, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI25453487, Length=425, Percent_Identity=31.7647058823529, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1790375, Length=419, Percent_Identity=32.4582338902148, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=26.5116279069767, Blast_Score=129, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71980708, Length=410, Percent_Identity=32.1951219512195, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=33.806146572104, Blast_Score=198, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=423, Percent_Identity=33.096926713948, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=47.0997679814385, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319307, Length=433, Percent_Identity=30.2540415704388, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321254, Length=448, Percent_Identity=26.7857142857143, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321170, Length=342, Percent_Identity=28.0701754385965, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321904, Length=411, Percent_Identity=24.330900243309, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=32.2274881516588, Blast_Score=197, Evalue=9e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46626; Mature: 46494

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00359 RIBOSOMAL_L11

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGQPAKQNNQRLETLAIHAGYTVEPTTKAVAVPIYHTSSYAFDNTQHGADLFDLKVAG
CCCCCCCCCCCCCEEEEEEECCCEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECC
NIYTRIMNPTNAVLEERVAALEGGIGALAVASGMAAITYAIQTICEAGDNIVAVSTLYGG
HHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TYNLFAHALPRQGIEVRFFDHKNPAAIRDLIDDKTKMVFAESIGNPLGNIIDIQALSDIA
HHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHHCCCCCCEEEHHHHHHHH
HEYGVPVVIDSTVATPALCRPFEFGADIVIHSLTKYMSGTGTSIGGAIVDSGTFPWDDYP
HHCCCCEEEECCCCCHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCC
ERFPLLNTPDASYHGVNFVKDVGAAAFIARARVAPLRNTGAALSPLNAFSILQGLQTLGL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
RMERHVQNAQAVAEYLRDHDKVAWVKYAGLPDHPEHELAKKYMKGTPSAILTFGVKGGLE
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHH
AGARFIDALQLITRLVNIGDAKSLACHPATTTHRQLNEQELEKAGVSIDMVRLSIGIEHI
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHCCHHHHHHCCCEEEEEEEHHHHHHH
EDIKADLEQALAAA
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SDGQPAKQNNQRLETLAIHAGYTVEPTTKAVAVPIYHTSSYAFDNTQHGADLFDLKVAG
CCCCCCCCCCCCEEEEEEECCCEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECC
NIYTRIMNPTNAVLEERVAALEGGIGALAVASGMAAITYAIQTICEAGDNIVAVSTLYGG
HHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TYNLFAHALPRQGIEVRFFDHKNPAAIRDLIDDKTKMVFAESIGNPLGNIIDIQALSDIA
HHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHHCCCCCCEEEHHHHHHHH
HEYGVPVVIDSTVATPALCRPFEFGADIVIHSLTKYMSGTGTSIGGAIVDSGTFPWDDYP
HHCCCCEEEECCCCCHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCC
ERFPLLNTPDASYHGVNFVKDVGAAAFIARARVAPLRNTGAALSPLNAFSILQGLQTLGL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
RMERHVQNAQAVAEYLRDHDKVAWVKYAGLPDHPEHELAKKYMKGTPSAILTFGVKGGLE
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHH
AGARFIDALQLITRLVNIGDAKSLACHPATTTHRQLNEQELEKAGVSIDMVRLSIGIEHI
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHCCHHHHHHCCCEEEEEEEHHHHHHH
EDIKADLEQALAAA
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]