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The map label for this gene is nfuA [H]

Identifier: 93007026

GI number: 93007026

Start: 2706331

End: 2707104

Strand: Reverse

Name: nfuA [H]

Synonym: Pcryo_2202

Alternate gene names: 93007026

Gene position: 2707104-2706331 (Counterclockwise)

Preceding gene: 93007031

Following gene: 93007025

Centisome position: 88.47

GC content: 44.96

Gene sequence:

>774_bases
ATGATAGTCACTGACACATATTCCCTTGCATTGACGACACAAGAAGCGGGTACACAGCACGCTGGTGTCATAACACAGAT
AGGACGCATTATGAGTGAACATAACCCAACGGATACTCAAGTAGAACCTGCTGCCGATTACGAGTCAGCGCTTGATACTG
AGCAAACCGAAGCTAAAAGCAATATTACCATCACGGAAGCGGCGCAAGGTTACTTAGCGGATTTGTTATCGAAGCAAGAC
ACCGATGGTATTGGCGTACGTATTTTTGTTGAACATCCGGGTACACCGCGTGCTGAGTGCTGCATGGCTTACAATCAGCC
AGGCGAAGAGGATAGTGCTGACCTGAAATTCAGCTACGATAATTTCTCTGCTTTTATTGAAGCGGCATCAGTACCTTATT
TAGAAGATGCGGTAATTGACTATAATAAAGACCGTTTTGGTGGTCAGTTGACCTTCCGTGCACCAAACTCTAAAGTGCCA
AAAGTCGGCGCTGATGCCAGTGTAGAAGAGCGTATCAACTATGTGTTGCAGTCTGAGATTAATCCAAGTCTGGCGGCGCA
TGGTGGTGATGTACAGTTGCTTGAATTGATTGATGAAGAAGGTGTTGGATTGACTGCCGTATTGAAATTTGGTGGCGGTT
GTCAAGGCTGTTCAGCGGTTGATATGACTTTGCGTCAAGGTGTTGAAGTGCAGCTAAAACAGCAAATACCAGAGCTTACT
CAAGTGATTGATGAGACCGATCATACTCGTACTGAAAACGCCTATTATAAATAA

Upstream 100 bases:

>100_bases
CTGCTAATTCACACCGCTGCAGTCGGAATAGTCGACTAAGTGCGCTATAATGAGCCGTATAAATAGATAGCTGTCATGGT
CAAAGCAACAAACGATAAGC

Downstream 100 bases:

>100_bases
GAATGCTTTGATTAATAAAACCTAATCGGTGATTATAAGTACGAATAGGATGTTTGTTTTTTATTGCTTATGAATTTTTG
TCATTAAGCGATGGATAATT

Product: HesB/YadR/YfhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MIVTDTYSLALTTQEAGTQHAGVITQIGRIMSEHNPTDTQVEPAADYESALDTEQTEAKSNITITEAAQGYLADLLSKQD
TDGIGVRIFVEHPGTPRAECCMAYNQPGEEDSADLKFSYDNFSAFIEAASVPYLEDAVIDYNKDRFGGQLTFRAPNSKVP
KVGADASVEERINYVLQSEINPSLAAHGGDVQLLELIDEEGVGLTAVLKFGGGCQGCSAVDMTLRQGVEVQLKQQIPELT
QVIDETDHTRTENAYYK

Sequences:

>Translated_257_residues
MIVTDTYSLALTTQEAGTQHAGVITQIGRIMSEHNPTDTQVEPAADYESALDTEQTEAKSNITITEAAQGYLADLLSKQD
TDGIGVRIFVEHPGTPRAECCMAYNQPGEEDSADLKFSYDNFSAFIEAASVPYLEDAVIDYNKDRFGGQLTFRAPNSKVP
KVGADASVEERINYVLQSEINPSLAAHGGDVQLLELIDEEGVGLTAVLKFGGGCQGCSAVDMTLRQGVEVQLKQQIPELT
QVIDETDHTRTENAYYK
>Mature_257_residues
MIVTDTYSLALTTQEAGTQHAGVITQIGRIMSEHNPTDTQVEPAADYESALDTEQTEAKSNITITEAAQGYLADLLSKQD
TDGIGVRIFVEHPGTPRAECCMAYNQPGEEDSADLKFSYDNFSAFIEAASVPYLEDAVIDYNKDRFGGQLTFRAPNSKVP
KVGADASVEERINYVLQSEINPSLAAHGGDVQLLELIDEEGVGLTAVLKFGGGCQGCSAVDMTLRQGVEVQLKQQIPELT
QVIDETDHTRTENAYYK

Specific function: Involved in iron-sulfur cluster biogenesis. Binds a 4Fe- 4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins [H]

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nfuA family [H]

Homologues:

Organism=Escherichia coli, GI1789819, Length=195, Percent_Identity=49.2307692307692, Blast_Score=194, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017726
- InterPro:   IPR000361
- InterPro:   IPR001075
- ProDom:   PD002830 [H]

Pfam domain/function: PF01521 Fe-S_biosyn; PF01106 NifU [H]

EC number: NA

Molecular weight: Translated: 27931; Mature: 27931

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTDTYSLALTTQEAGTQHAGVITQIGRIMSEHNPTDTQVEPAADYESALDTEQTEAKS
CEEECCEEEEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHCC
NITITEAAQGYLADLLSKQDTDGIGVRIFVEHPGTPRAECCMAYNQPGEEDSADLKFSYD
CEEEEHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCEEEECC
NFSAFIEAASVPYLEDAVIDYNKDRFGGQLTFRAPNSKVPKVGADASVEERINYVLQSEI
HHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
NPSLAAHGGDVQLLELIDEEGVGLTAVLKFGGGCQGCSAVDMTLRQGVEVQLKQQIPELT
CCHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
QVIDETDHTRTENAYYK
HHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIVTDTYSLALTTQEAGTQHAGVITQIGRIMSEHNPTDTQVEPAADYESALDTEQTEAKS
CEEECCEEEEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHCC
NITITEAAQGYLADLLSKQDTDGIGVRIFVEHPGTPRAECCMAYNQPGEEDSADLKFSYD
CEEEEHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCEEEECC
NFSAFIEAASVPYLEDAVIDYNKDRFGGQLTFRAPNSKVPKVGADASVEERINYVLQSEI
HHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
NPSLAAHGGDVQLLELIDEEGVGLTAVLKFGGGCQGCSAVDMTLRQGVEVQLKQQIPELT
CCHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
QVIDETDHTRTENAYYK
HHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA