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The map label for this gene is estA [H]

Identifier: 93006457

GI number: 93006457

Start: 2008740

End: 2009411

Strand: Reverse

Name: estA [H]

Synonym: Pcryo_1633

Alternate gene names: 93006457

Gene position: 2009411-2008740 (Counterclockwise)

Preceding gene: 93006459

Following gene: 93006456

Centisome position: 65.67

GC content: 47.62

Gene sequence:

>672_bases
ATGAATAAGTATCTAGATGCCGTTATTGTCGAACACAATCATGCAAACAAAGCGATTAATCGATCCGTGATTTGGCTGCA
TGGTTTGGGGGCTAGCGGTCATGACTTTGAGCCAGTCGTGCCGCAGCTCGGCTTAGCGGATGGAATGGCAGTGCGGTTTA
TCTTTCCGCATGCACCCAACCGCCCAGTGACGGTCAATGGCGGTATGGTCATGCCCGCATGGTATGATATCCTGGAGATG
AGCCTTGAGCGCAAAGTTGATATTGCGCAGATTGAAGAGTCCGCACAACAAATCCATGACTTGATTGCTCGTGAAATCGA
GCGCGGTGTGAAACCTGAACATATTGTGATTGCAGGATTTTCGCAGGGCGGGGCAGTGGCGTATCATGTGGCACTCGGTT
ACCCACAGCGCTTGGCTGGCTTGATGGCGCTTTCTACCTACTTTGCAACCAATGACAGCGTTCGCTATAGCGCTGTCAAC
ATAGATATGCCAATTTTGATCGAGCATGGTACGCATGATCCAGTTGTTCCCGTTATATTAGGCGAACAAGCAAAGCAGTT
ATTATCAGAGAAAGGATATAATGTTGCTTATCATACCTATCCTATGGCCCATCAGGTATGTATGCCGCAGATTCAAAATA
TCGGTAAATGGTTAAATACCATACTGGCTTAG

Upstream 100 bases:

>100_bases
GTTCCTAAAATATAGGGTTATGTTTAAGGAATGATGACAACCAAAGTCATTTTGTAATATTAATTTTGTTTAAATTTTTG
ACTCTCATTAAGGATAATTA

Downstream 100 bases:

>100_bases
ATGATTAAGCTGTAACGTAATGTAATACAGAGCTCTAATAGGTTGCAAGCAGCCGTATATATGCTATATAATCATCGACC
TTATTGGGGATGTTCTGGCT

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MNKYLDAVIVEHNHANKAINRSVIWLHGLGASGHDFEPVVPQLGLADGMAVRFIFPHAPNRPVTVNGGMVMPAWYDILEM
SLERKVDIAQIEESAQQIHDLIAREIERGVKPEHIVIAGFSQGGAVAYHVALGYPQRLAGLMALSTYFATNDSVRYSAVN
IDMPILIEHGTHDPVVPVILGEQAKQLLSEKGYNVAYHTYPMAHQVCMPQIQNIGKWLNTILA

Sequences:

>Translated_223_residues
MNKYLDAVIVEHNHANKAINRSVIWLHGLGASGHDFEPVVPQLGLADGMAVRFIFPHAPNRPVTVNGGMVMPAWYDILEM
SLERKVDIAQIEESAQQIHDLIAREIERGVKPEHIVIAGFSQGGAVAYHVALGYPQRLAGLMALSTYFATNDSVRYSAVN
IDMPILIEHGTHDPVVPVILGEQAKQLLSEKGYNVAYHTYPMAHQVCMPQIQNIGKWLNTILA
>Mature_223_residues
MNKYLDAVIVEHNHANKAINRSVIWLHGLGASGHDFEPVVPQLGLADGMAVRFIFPHAPNRPVTVNGGMVMPAWYDILEM
SLERKVDIAQIEESAQQIHDLIAREIERGVKPEHIVIAGFSQGGAVAYHVALGYPQRLAGLMALSTYFATNDSVRYSAVN
IDMPILIEHGTHDPVVPVILGEQAKQLLSEKGYNVAYHTYPMAHQVCMPQIQNIGKWLNTILA

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=236, Percent_Identity=34.3220338983051, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI9966764, Length=206, Percent_Identity=36.4077669902913, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI20270341, Length=208, Percent_Identity=29.8076923076923, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17508057, Length=204, Percent_Identity=34.8039215686275, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323147, Length=212, Percent_Identity=34.9056603773585, Blast_Score=129, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45553055, Length=218, Percent_Identity=34.8623853211009, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI45553057, Length=218, Percent_Identity=34.8623853211009, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI21357257, Length=218, Percent_Identity=34.8623853211009, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI161078179, Length=207, Percent_Identity=27.0531400966184, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24634; Mature: 24634

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKYLDAVIVEHNHANKAINRSVIWLHGLGASGHDFEPVVPQLGLADGMAVRFIFPHAPN
CCCCEEEEEEECCCCCHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
RPVTVNGGMVMPAWYDILEMSLERKVDIAQIEESAQQIHDLIAREIERGVKPEHIVIAGF
CCEEEECCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SQGGAVAYHVALGYPQRLAGLMALSTYFATNDSVRYSAVNIDMPILIEHGTHDPVVPVIL
CCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCEEEEEE
GEQAKQLLSEKGYNVAYHTYPMAHQVCMPQIQNIGKWLNTILA
CHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKYLDAVIVEHNHANKAINRSVIWLHGLGASGHDFEPVVPQLGLADGMAVRFIFPHAPN
CCCCEEEEEEECCCCCHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
RPVTVNGGMVMPAWYDILEMSLERKVDIAQIEESAQQIHDLIAREIERGVKPEHIVIAGF
CCEEEECCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SQGGAVAYHVALGYPQRLAGLMALSTYFATNDSVRYSAVNIDMPILIEHGTHDPVVPVIL
CCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCEEEEEE
GEQAKQLLSEKGYNVAYHTYPMAHQVCMPQIQNIGKWLNTILA
CHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA