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The map label for this gene is intS [H]

Identifier: 93006456

GI number: 93006456

Start: 2006891

End: 2008132

Strand: Reverse

Name: intS [H]

Synonym: Pcryo_1632

Alternate gene names: 93006456

Gene position: 2008132-2006891 (Counterclockwise)

Preceding gene: 93006457

Following gene: 93006455

Centisome position: 65.63

GC content: 44.69

Gene sequence:

>1242_bases
ATGCCCTTAACTCATACCGTTATCAATAAGCTACAGCCCAGTAGCACCTCGATTGATACAAAGCGACCTGATAAACATAG
TGATGGTAACGGGCTGCAATTATGGGTTCGTTACACGGGCGTTAAGTCATGGATCAGCGCCTACCGCTGGCAAGGTAAGC
AGCAAACCCTCACTATTGGCACTTATCCAGTTATGAGCTTGCAGAACGCAAGGCAGCGCAATATTGAGATCAAACGCTTA
ATAGCTGATGGTGTGAACCCCAAAGATCATAAAAAAGAGCAGCAAGCCAGCCAAGACGGTTTAAATATTTTCGATAACAT
AGCCCAGTCATGGTATGCAGAACGCAAAACCTACCTAGCAGAAAGCACCTTTACTCGTAACTATTCCGCCTATATGCGCG
ATGTTAAGCCCTCTATTGGTCAAAAGAACATCAACGACATAACCGCGCCTGATATATTAGCTATTGGCAAAGCAGTAGAA
AGCCGTGGTGCTAATGAAATGGCAAGGCGTACCATTAGAGAAGTAGGACAGATATTTAAGCACGCTATCCGCAATGGACT
AGCCACACATAACCCAGCCACCGACCTAGCCGAAGCAATCAAACCACACAAAACGACCAACCACCACCGTATTACTAGCC
AGCAACTGCCAAAGCTACTAACAGACATCAATGCTTATCAAGGTGATGTACTGGTGAAACTAGGCTTATGGTTTTTATGC
TATACGTTTGTGCGTACTAATGAGATCCGCTTCATGGAGTGGTCGGAGATTGACTACAAACATGGCTTATGGCGCATACC
AGCCGATAAGATGAAAGCCAAGCGTTTGCATATTGTGCCATTAGCACCGCAAGCAATGGCGATACTGGAACAGATCAAAG
AGCTAGGTTTTTCCGATCAGTACGTTTTCTTTAATACCTCAACTCGTAAGCCATACAGTCAAAGCGCGTTTATTAATGCC
CTATGGAATATGGGTTATAAAGGCAAGATGACGGGGCACGGGTTTAGAGGGCTTGCCAGTACCACACTACATGAAAAAGA
GTTTATGCACGAAGCCATAGAGCTACAGCTGGCACATGATAGAGAAAACAAGATCAGCGCAGCCTACAACGGGGCGCAGC
ACTTGCCATATAGAATAGACATGATGCACCAATGGGCAAACTTTATTGATGATGCTTATGCTGGCAAATTGGATAACGTC
ATACATACCAACTTTAGACAACAAAAACAAAAGCATGGCTAA

Upstream 100 bases:

>100_bases
TTGAGGGTTATGTAGGGGTATAGCGTCCTATATAGAATGTTGGTATTATTGTTGGTATTAGAGTTTATACATATGCTCAA
TACCAACAAAGGATAGTCCA

Downstream 100 bases:

>100_bases
TATCATGCTGTAACGACTATGCAAGGCTAGGACTAATTACCCGAAAGCCAGCCGTTCACACACCTTTAGGCTGGCACTGC
CTTGCACCTACTTTGAATTA

Product: phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MPLTHTVINKLQPSSTSIDTKRPDKHSDGNGLQLWVRYTGVKSWISAYRWQGKQQTLTIGTYPVMSLQNARQRNIEIKRL
IADGVNPKDHKKEQQASQDGLNIFDNIAQSWYAERKTYLAESTFTRNYSAYMRDVKPSIGQKNINDITAPDILAIGKAVE
SRGANEMARRTIREVGQIFKHAIRNGLATHNPATDLAEAIKPHKTTNHHRITSQQLPKLLTDINAYQGDVLVKLGLWFLC
YTFVRTNEIRFMEWSEIDYKHGLWRIPADKMKAKRLHIVPLAPQAMAILEQIKELGFSDQYVFFNTSTRKPYSQSAFINA
LWNMGYKGKMTGHGFRGLASTTLHEKEFMHEAIELQLAHDRENKISAAYNGAQHLPYRIDMMHQWANFIDDAYAGKLDNV
IHTNFRQQKQKHG

Sequences:

>Translated_413_residues
MPLTHTVINKLQPSSTSIDTKRPDKHSDGNGLQLWVRYTGVKSWISAYRWQGKQQTLTIGTYPVMSLQNARQRNIEIKRL
IADGVNPKDHKKEQQASQDGLNIFDNIAQSWYAERKTYLAESTFTRNYSAYMRDVKPSIGQKNINDITAPDILAIGKAVE
SRGANEMARRTIREVGQIFKHAIRNGLATHNPATDLAEAIKPHKTTNHHRITSQQLPKLLTDINAYQGDVLVKLGLWFLC
YTFVRTNEIRFMEWSEIDYKHGLWRIPADKMKAKRLHIVPLAPQAMAILEQIKELGFSDQYVFFNTSTRKPYSQSAFINA
LWNMGYKGKMTGHGFRGLASTTLHEKEFMHEAIELQLAHDRENKISAAYNGAQHLPYRIDMMHQWANFIDDAYAGKLDNV
IHTNFRQQKQKHG
>Mature_412_residues
PLTHTVINKLQPSSTSIDTKRPDKHSDGNGLQLWVRYTGVKSWISAYRWQGKQQTLTIGTYPVMSLQNARQRNIEIKRLI
ADGVNPKDHKKEQQASQDGLNIFDNIAQSWYAERKTYLAESTFTRNYSAYMRDVKPSIGQKNINDITAPDILAIGKAVES
RGANEMARRTIREVGQIFKHAIRNGLATHNPATDLAEAIKPHKTTNHHRITSQQLPKLLTDINAYQGDVLVKLGLWFLCY
TFVRTNEIRFMEWSEIDYKHGLWRIPADKMKAKRLHIVPLAPQAMAILEQIKELGFSDQYVFFNTSTRKPYSQSAFINAL
WNMGYKGKMTGHGFRGLASTTLHEKEFMHEAIELQLAHDRENKISAAYNGAQHLPYRIDMMHQWANFIDDAYAGKLDNVI
HTNFRQQKQKHG

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=373, Percent_Identity=33.5120643431635, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI145693166, Length=402, Percent_Identity=36.81592039801, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1788974, Length=401, Percent_Identity=31.6708229426434, Blast_Score=201, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47434; Mature: 47302

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTHTVINKLQPSSTSIDTKRPDKHSDGNGLQLWVRYTGVKSWISAYRWQGKQQTLTIG
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCEEEEE
TYPVMSLQNARQRNIEIKRLIADGVNPKDHKKEQQASQDGLNIFDNIAQSWYAERKTYLA
CCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ESTFTRNYSAYMRDVKPSIGQKNINDITAPDILAIGKAVESRGANEMARRTIREVGQIFK
HHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
HAIRNGLATHNPATDLAEAIKPHKTTNHHRITSQQLPKLLTDINAYQGDVLVKLGLWFLC
HHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YTFVRTNEIRFMEWSEIDYKHGLWRIPADKMKAKRLHIVPLAPQAMAILEQIKELGFSDQ
HHHHHCCCEEEEECCCCCCCCCCEECCHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCC
YVFFNTSTRKPYSQSAFINALWNMGYKGKMTGHGFRGLASTTLHEKEFMHEAIELQLAHD
EEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RENKISAAYNGAQHLPYRIDMMHQWANFIDDAYAGKLDNVIHTNFRQQKQKHG
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLTHTVINKLQPSSTSIDTKRPDKHSDGNGLQLWVRYTGVKSWISAYRWQGKQQTLTIG
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCEEEEE
TYPVMSLQNARQRNIEIKRLIADGVNPKDHKKEQQASQDGLNIFDNIAQSWYAERKTYLA
CCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ESTFTRNYSAYMRDVKPSIGQKNINDITAPDILAIGKAVESRGANEMARRTIREVGQIFK
HHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
HAIRNGLATHNPATDLAEAIKPHKTTNHHRITSQQLPKLLTDINAYQGDVLVKLGLWFLC
HHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YTFVRTNEIRFMEWSEIDYKHGLWRIPADKMKAKRLHIVPLAPQAMAILEQIKELGFSDQ
HHHHHCCCEEEEECCCCCCCCCCEECCHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCC
YVFFNTSTRKPYSQSAFINALWNMGYKGKMTGHGFRGLASTTLHEKEFMHEAIELQLAHD
EEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RENKISAAYNGAQHLPYRIDMMHQWANFIDDAYAGKLDNVIHTNFRQQKQKHG
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]