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The map label for this gene is ilvA [H]

Identifier: 93006293

GI number: 93006293

Start: 1807541

End: 1809094

Strand: Direct

Name: ilvA [H]

Synonym: Pcryo_1467

Alternate gene names: 93006293

Gene position: 1807541-1809094 (Clockwise)

Preceding gene: 93006287

Following gene: 93006294

Centisome position: 59.07

GC content: 46.33

Gene sequence:

>1554_bases
ATGCTGTCACATTGGGTACGAGCCATCTTGCAAGCCACCGTCTATGACGTTGCTATTCAAACACCACTTGAAGCGGCACC
CAAACTGACCCAACGTTTTACTAATGACATTCGTTTTAAACGTGAAGACCTGCAACCGGTAAAGTCCTTTAAACTGCGCG
GCGCTTATAACCGCATTAGCCAATTAAATGATGAGCAAAAAGCACGTGGCGTGATTTGCGCATCGGCAGGTAACCATGCC
CAAGGGGTTGCCTACTCTGCTCGTAAACTTGCTCTTAATAACATTATCGTTATGCCAACTACTACTCCTGATATCAAAGT
AGATGCCGTCAGAGCACTTGGCGGCAATGTCGACTTGTACGGTGATAGCTTTGATGAAGCCAATCGCTATGCCATTGATC
GCGCTGAGCGTGAAGGTCTGACTTTTATCCCACCATATGATGATGAGCTGGTGATTGCTGGACAAGGTACCATCGGGCTT
GAGCTCACCCAGCAATGGCGCAATATGGATTACGTTTTTGTAGCAGTTGGTGGTGGTGGTCTGATTTCTGGCGTCGCTGC
ATTTTTGGGCGAAGTTGCCCCGCATGTCAAAGTCATCGCTGTAGAAGCCGACCAATCAGCGTCACTAAAAGCGGCGCTTG
AAGCCAATGAGCGAGTCAAACTTGAACAAGTAGGACTGTTCGTCGATGGTGTGGCAGTTGCCCAAATCGGCGAATTACCG
TTTGATATTGTTCGTATGCAGAAGAGCGATAAGTCCGGTCCTGTCGTCGATCCTGAAGTCGTCACTTGTACCAATGATGA
AGTTTGCGCTGCTGTCAAAGATATCTTTGAAGAGAACCGTAGTATCGTTGAGCCTGCTGGCGCGCTGCCAGTTGCTGGTA
TGAAAAAATACATCGAGGCTCATAACCTACAAGGCAAAAACTGCGTCGCCATCATTTGCGGTGCTAATATGAATTTTGAT
CGCTTGCGCTACATCGCTGAGCGTACTGAGATTGGTGAGAAAAAAGAAGCCATCTTTGGCGTAACGATCCCTGAGCAGAC
TGGCGCTTTCTTAAACTTCTGTCGTAGCCTACACGGTCGTAATATTACTGAGTTTAATTACCGTGCTGATAGTAAAAAAT
CACCTGATTCTATGGAACCTGCGGCGATATTCGTTGGGATTGCATTAAAAGAAGGTGACAAAGAGCGTCAAACCATCCGT
ACGCAATTGGTAGCTGATGGCTATACAGCACACGACTTGACCGATGACGATGTCGCCAAATCCCATATCCGCCATCTGAT
TGGTGGCCATGCGCATGTTGAAAATGAGCAATTATTACAAGTCACTTTCCCAGAACGTCCAGGTGCTTTATTGACCTTTT
TAGAAAAATTGGGTGACGATTTTAATATTACCTTGTTTCATTATCGCAATCATGGCGCTGCTGAAGGTCGAGTATTGGTC
GGTTTACAAGCTTCTGAGGGTAACTCTCGCCAGCTACAAGATGCGCTGCTCGATATCGGTTACGACTGCACCATGCTTAA
TGATAATGTTGGTTATCAGTTGTTTTTGAAATAA

Upstream 100 bases:

>100_bases
ATTTTTATCATTCTCTGCATTTTTTGTCGCTGATAGCGACTCGCTTGTTTACTTATAATTCACCGCACTTTTTCATACAT
ACTCTAGTTTAGGAATCATT

Downstream 100 bases:

>100_bases
AACTAAACATAGACTATCAATTCATCAGTGGCCGAAAGCATTGCTCCCAAATGACATAATGCACAGAAGCCACTTAAATC
TTATAGCACTGTCCTAATAC

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MLSHWVRAILQATVYDVAIQTPLEAAPKLTQRFTNDIRFKREDLQPVKSFKLRGAYNRISQLNDEQKARGVICASAGNHA
QGVAYSARKLALNNIIVMPTTTPDIKVDAVRALGGNVDLYGDSFDEANRYAIDRAEREGLTFIPPYDDELVIAGQGTIGL
ELTQQWRNMDYVFVAVGGGGLISGVAAFLGEVAPHVKVIAVEADQSASLKAALEANERVKLEQVGLFVDGVAVAQIGELP
FDIVRMQKSDKSGPVVDPEVVTCTNDEVCAAVKDIFEENRSIVEPAGALPVAGMKKYIEAHNLQGKNCVAIICGANMNFD
RLRYIAERTEIGEKKEAIFGVTIPEQTGAFLNFCRSLHGRNITEFNYRADSKKSPDSMEPAAIFVGIALKEGDKERQTIR
TQLVADGYTAHDLTDDDVAKSHIRHLIGGHAHVENEQLLQVTFPERPGALLTFLEKLGDDFNITLFHYRNHGAAEGRVLV
GLQASEGNSRQLQDALLDIGYDCTMLNDNVGYQLFLK

Sequences:

>Translated_517_residues
MLSHWVRAILQATVYDVAIQTPLEAAPKLTQRFTNDIRFKREDLQPVKSFKLRGAYNRISQLNDEQKARGVICASAGNHA
QGVAYSARKLALNNIIVMPTTTPDIKVDAVRALGGNVDLYGDSFDEANRYAIDRAEREGLTFIPPYDDELVIAGQGTIGL
ELTQQWRNMDYVFVAVGGGGLISGVAAFLGEVAPHVKVIAVEADQSASLKAALEANERVKLEQVGLFVDGVAVAQIGELP
FDIVRMQKSDKSGPVVDPEVVTCTNDEVCAAVKDIFEENRSIVEPAGALPVAGMKKYIEAHNLQGKNCVAIICGANMNFD
RLRYIAERTEIGEKKEAIFGVTIPEQTGAFLNFCRSLHGRNITEFNYRADSKKSPDSMEPAAIFVGIALKEGDKERQTIR
TQLVADGYTAHDLTDDDVAKSHIRHLIGGHAHVENEQLLQVTFPERPGALLTFLEKLGDDFNITLFHYRNHGAAEGRVLV
GLQASEGNSRQLQDALLDIGYDCTMLNDNVGYQLFLK
>Mature_517_residues
MLSHWVRAILQATVYDVAIQTPLEAAPKLTQRFTNDIRFKREDLQPVKSFKLRGAYNRISQLNDEQKARGVICASAGNHA
QGVAYSARKLALNNIIVMPTTTPDIKVDAVRALGGNVDLYGDSFDEANRYAIDRAEREGLTFIPPYDDELVIAGQGTIGL
ELTQQWRNMDYVFVAVGGGGLISGVAAFLGEVAPHVKVIAVEADQSASLKAALEANERVKLEQVGLFVDGVAVAQIGELP
FDIVRMQKSDKSGPVVDPEVVTCTNDEVCAAVKDIFEENRSIVEPAGALPVAGMKKYIEAHNLQGKNCVAIICGANMNFD
RLRYIAERTEIGEKKEAIFGVTIPEQTGAFLNFCRSLHGRNITEFNYRADSKKSPDSMEPAAIFVGIALKEGDKERQTIR
TQLVADGYTAHDLTDDDVAKSHIRHLIGGHAHVENEQLLQVTFPERPGALLTFLEKLGDDFNITLFHYRNHGAAEGRVLV
GLQASEGNSRQLQDALLDIGYDCTMLNDNVGYQLFLK

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=310, Percent_Identity=31.6129032258064, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI33469958, Length=321, Percent_Identity=29.9065420560748, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI19923959, Length=325, Percent_Identity=30.4615384615385, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1790207, Length=516, Percent_Identity=50, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789505, Length=307, Percent_Identity=35.5048859934853, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71991565, Length=308, Percent_Identity=32.4675324675325, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17508781, Length=285, Percent_Identity=30.8771929824561, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17537387, Length=332, Percent_Identity=28.9156626506024, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320930, Length=522, Percent_Identity=47.8927203065134, Blast_Score=445, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322631, Length=326, Percent_Identity=33.4355828220859, Blast_Score=146, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6319788, Length=269, Percent_Identity=24.5353159851301, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355833, Length=334, Percent_Identity=30.8383233532934, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24645328, Length=260, Percent_Identity=28.4615384615385, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 56729; Mature: 56729

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHWVRAILQATVYDVAIQTPLEAAPKLTQRFTNDIRFKREDLQPVKSFKLRGAYNRIS
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHH
QLNDEQKARGVICASAGNHAQGVAYSARKLALNNIIVMPTTTPDIKVDAVRALGGNVDLY
HCCCHHHHCEEEEECCCCCCCCHHHHHHHEEECCEEEEECCCCCCEEEEHHHCCCCEEEC
GDSFDEANRYAIDRAEREGLTFIPPYDDELVIAGQGTIGLELTQQWRNMDYVFVAVGGGG
CCCCCCCCHHHHHHHHHCCCEEECCCCCCEEEECCCCEEHHHHHHHCCCCEEEEEECCCH
LISGVAAFLGEVAPHVKVIAVEADQSASLKAALEANERVKLEQVGLFVDGVAVAQIGELP
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCCCCEEHHHHCEEECCHHHHHHCCCC
FDIVRMQKSDKSGPVVDPEVVTCTNDEVCAAVKDIFEENRSIVEPAGALPVAGMKKYIEA
HHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHH
HNLQGKNCVAIICGANMNFDRLRYIAERTEIGEKKEAIFGVTIPEQTGAFLNFCRSLHGR
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
NITEFNYRADSKKSPDSMEPAAIFVGIALKEGDKERQTIRTQLVADGYTAHDLTDDDVAK
CCEEECCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
SHIRHLIGGHAHVENEQLLQVTFPERPGALLTFLEKLGDDFNITLFHYRNHGAAEGRVLV
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEE
GLQASEGNSRQLQDALLDIGYDCTMLNDNVGYQLFLK
EEECCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEC
>Mature Secondary Structure
MLSHWVRAILQATVYDVAIQTPLEAAPKLTQRFTNDIRFKREDLQPVKSFKLRGAYNRIS
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHH
QLNDEQKARGVICASAGNHAQGVAYSARKLALNNIIVMPTTTPDIKVDAVRALGGNVDLY
HCCCHHHHCEEEEECCCCCCCCHHHHHHHEEECCEEEEECCCCCCEEEEHHHCCCCEEEC
GDSFDEANRYAIDRAEREGLTFIPPYDDELVIAGQGTIGLELTQQWRNMDYVFVAVGGGG
CCCCCCCCHHHHHHHHHCCCEEECCCCCCEEEECCCCEEHHHHHHHCCCCEEEEEECCCH
LISGVAAFLGEVAPHVKVIAVEADQSASLKAALEANERVKLEQVGLFVDGVAVAQIGELP
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCCCCEEHHHHCEEECCHHHHHHCCCC
FDIVRMQKSDKSGPVVDPEVVTCTNDEVCAAVKDIFEENRSIVEPAGALPVAGMKKYIEA
HHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHH
HNLQGKNCVAIICGANMNFDRLRYIAERTEIGEKKEAIFGVTIPEQTGAFLNFCRSLHGR
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
NITEFNYRADSKKSPDSMEPAAIFVGIALKEGDKERQTIRTQLVADGYTAHDLTDDDVAK
CCEEECCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
SHIRHLIGGHAHVENEQLLQVTFPERPGALLTFLEKLGDDFNITLFHYRNHGAAEGRVLV
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEE
GLQASEGNSRQLQDALLDIGYDCTMLNDNVGYQLFLK
EEECCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]