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The map label for this gene is rsmE [H]

Identifier: 93006276

GI number: 93006276

Start: 1785018

End: 1785917

Strand: Direct

Name: rsmE [H]

Synonym: Pcryo_1450

Alternate gene names: 93006276

Gene position: 1785018-1785917 (Clockwise)

Preceding gene: 93006275

Following gene: 93006277

Centisome position: 58.34

GC content: 46.56

Gene sequence:

>900_bases
TTGCGACGTTTCTTTTATGCCACTAATAGCAACATTAGTAATGACGTTACCAATGAAAAGGCTGCGACCTACCCTCAGCT
TAGCACCTTGCCTATCGGTGCCAGCGTCGAATTAACCGAAAGTATCATCCATCATTGGTGCCGTGTATTACGCGCTAATA
TTGGCGATAAAGGTATCTTATTTGACGGTTTTGGCGGTGAATATCAGGTACAGATACTGACCATCAGTAAAAAAAACGCG
ACTGTGACCCTGCTTTCTCACACAAATAATGATAATAGTGCACCAATATTTACCAAAATCGGGCTGGTAATGAGTCGTGG
CGAACGGATGGATTACGCCATTCAAAAATCTACCGAGCTTGGCGTCACTGCCATCCAATTGCTAAGCAGTCATCATGGCG
AGGTCAACCTGAAGCCTGCCCAAGTCGAAAAAAAGCTTGCTCATTGGCAGCAGGTTGCGATTGCTGCCTGTGAGCAATGC
GGTCTTAACCGTCCACCGCTTATTATTGCGCCTATTCCCATTAACGATTGGCTTAAGCCTATCACAACGACCACGCCTGT
GAAGATAGAAGCGACTATCAGCTCTATGGTTACCACCCTTAGTCAGGATGCTTATTATCAAGTATTACAACAACCCGCTG
ATTTGCGTCTACAGCTTAGTGTACCAGCGGCAGGACAACCTGCTATGCCTGATTCGCTACTATCGGTATTACAGCAACCA
GTGCCTTATATTGAATTGTTGATTGGTCCTGAAGGTGGCCTAAGTGCTAACGAATGCGCTCAAGCAGCAGACGTTGGTTT
TGAGCCTTGGCAAATTGGCACAAGGGTATTACGAACAGAAACTGCGCCAGTAGTCGCCTTGGCAACATTGCAAGCGCTGT
TGCCACGTGTCAATCCTTAG

Upstream 100 bases:

>100_bases
TTATGCGAATTCCATTATTGACAATCCCTTTACCCACTCAAATGATATTCTGCTAAGATATTTAAAAATATCATGACCCC
CATTATAGGAATAAGTAATT

Downstream 100 bases:

>100_bases
TAGCAGTGCAAAGCGTTATATAGTCAGTTCACAATAAAAGATACAAATGAAATAGCGTTTTACTGAGACAAATTTCGCTT
GCTGTGCACTATGCGTATGA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRRFFYATNSNISNDVTNEKAATYPQLSTLPIGASVELTESIIHHWCRVLRANIGDKGILFDGFGGEYQVQILTISKKNA
TVTLLSHTNNDNSAPIFTKIGLVMSRGERMDYAIQKSTELGVTAIQLLSSHHGEVNLKPAQVEKKLAHWQQVAIAACEQC
GLNRPPLIIAPIPINDWLKPITTTTPVKIEATISSMVTTLSQDAYYQVLQQPADLRLQLSVPAAGQPAMPDSLLSVLQQP
VPYIELLIGPEGGLSANECAQAADVGFEPWQIGTRVLRTETAPVVALATLQALLPRVNP

Sequences:

>Translated_299_residues
MRRFFYATNSNISNDVTNEKAATYPQLSTLPIGASVELTESIIHHWCRVLRANIGDKGILFDGFGGEYQVQILTISKKNA
TVTLLSHTNNDNSAPIFTKIGLVMSRGERMDYAIQKSTELGVTAIQLLSSHHGEVNLKPAQVEKKLAHWQQVAIAACEQC
GLNRPPLIIAPIPINDWLKPITTTTPVKIEATISSMVTTLSQDAYYQVLQQPADLRLQLSVPAAGQPAMPDSLLSVLQQP
VPYIELLIGPEGGLSANECAQAADVGFEPWQIGTRVLRTETAPVVALATLQALLPRVNP
>Mature_299_residues
MRRFFYATNSNISNDVTNEKAATYPQLSTLPIGASVELTESIIHHWCRVLRANIGDKGILFDGFGGEYQVQILTISKKNA
TVTLLSHTNNDNSAPIFTKIGLVMSRGERMDYAIQKSTELGVTAIQLLSSHHGEVNLKPAQVEKKLAHWQQVAIAACEQC
GLNRPPLIIAPIPINDWLKPITTTTPVKIEATISSMVTTLSQDAYYQVLQQPADLRLQLSVPAAGQPAMPDSLLSVLQQP
VPYIELLIGPEGGLSANECAQAADVGFEPWQIGTRVLRTETAPVVALATLQALLPRVNP

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=258, Percent_Identity=36.8217054263566, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 32525; Mature: 32525

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFFYATNSNISNDVTNEKAATYPQLSTLPIGASVELTESIIHHWCRVLRANIGDKGIL
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
FDGFGGEYQVQILTISKKNATVTLLSHTNNDNSAPIFTKIGLVMSRGERMDYAIQKSTEL
EECCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCC
GVTAIQLLSSHHGEVNLKPAQVEKKLAHWQQVAIAACEQCGLNRPPLIIAPIPINDWLKP
HHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHC
ITTTTPVKIEATISSMVTTLSQDAYYQVLQQPADLRLQLSVPAAGQPAMPDSLLSVLQQP
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
VPYIELLIGPEGGLSANECAQAADVGFEPWQIGTRVLRTETAPVVALATLQALLPRVNP
CCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRRFFYATNSNISNDVTNEKAATYPQLSTLPIGASVELTESIIHHWCRVLRANIGDKGIL
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
FDGFGGEYQVQILTISKKNATVTLLSHTNNDNSAPIFTKIGLVMSRGERMDYAIQKSTEL
EECCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCC
GVTAIQLLSSHHGEVNLKPAQVEKKLAHWQQVAIAACEQCGLNRPPLIIAPIPINDWLKP
HHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHC
ITTTTPVKIEATISSMVTTLSQDAYYQVLQQPADLRLQLSVPAAGQPAMPDSLLSVLQQP
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
VPYIELLIGPEGGLSANECAQAADVGFEPWQIGTRVLRTETAPVVALATLQALLPRVNP
CCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]