Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 93006275

GI number: 93006275

Start: 1783996

End: 1784844

Strand: Direct

Name: metF [H]

Synonym: Pcryo_1449

Alternate gene names: 93006275

Gene position: 1783996-1784844 (Clockwise)

Preceding gene: 93006274

Following gene: 93006276

Centisome position: 58.3

GC content: 46.76

Gene sequence:

>849_bases
GTGACTAAGCCTGCTTTCTCCTTTGAGTTTTTCCCAGCCAAAACCGAGCAAGGACACGAAAAGCTCCTCAGCACTTATGA
TGAGCTGAATAAGCTGTCACCTGCGTACTTTTCGGTGACCTATGGTGCTGGTGGTTCAACTCGTAGCCGCACTTTAGATA
TTGTCCAAGCCTTATGCGCGCGCGGTGAGACTGATATTGCGCCGCATATGTCTTGTATTGGCGATGATAAAAGTGAAATT
GCCGAGTTACTTGAGCATTACAAAAGCTTGGGCATCAAACGTTTGGTCGCATTACGTGGCGATTTGCCTTCAGGTCAAGT
AGGCATGGGCGAGCTGCCGTTTGCATTAGATTTGGTCAAGTTCATCCGTGAGCATTCGGGCGATCATTTCCATATCGAAG
TAGCCGCCTATCCTGAGATGCATCCACAAGCGCGTAGCTTTAAGCTTGATGTCGACAACTTGGTCAATAAATATCAAGCT
GGCGCCAATGCTTCGATTACCCAGTTTTTCTACAACGCTGATAGCTACTTGTATCTGCGTGAGAGCTTAGAAAAACGCGG
TATTGATACCGTAGCACAGCCATTGGTTGCTGGCATTATGCCCATCACCAACTCTAGCAATCTGATGCGTTTTGCTGACA
GCTGTGGCGCCGATATTCCGCGTTATGTACGTAAGCAATTGGCTGACTTTGGTGATGATAGTAAGGCGATTCGCGAGTTT
GGCTTTGAAGTGGTCTATCGTCTGTGTGAGCGTTTAATTAGCGAGGGTGTACCTGCCATGCATTTTTATAGCATGAATAA
GGTTGAGCCAAACAAGCGCTTAATTGAAGCCTTAGGCTTGATTGCTTAA

Upstream 100 bases:

>100_bases
ACGCTAACCAAGTTGACTGAAAAGCAAGCTGAGTATTTAGGCGTACCTGTTGAAGGTCCATTTAAGCCTGACGCTTATAA
ATACTAAAAGGAGTCAGACT

Downstream 100 bases:

>100_bases
ATATTTACCGAATGTATTGCTTAATGACTGGCGTTCCTAGGAGCGCCAGTCTTTTTTGGTATGGGAATTGCAGTTATGCG
AATTCCATTATTGACAATCC

Product: 5,10-methylenetetrahydrofolate reductase (FADH)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTKPAFSFEFFPAKTEQGHEKLLSTYDELNKLSPAYFSVTYGAGGSTRSRTLDIVQALCARGETDIAPHMSCIGDDKSEI
AELLEHYKSLGIKRLVALRGDLPSGQVGMGELPFALDLVKFIREHSGDHFHIEVAAYPEMHPQARSFKLDVDNLVNKYQA
GANASITQFFYNADSYLYLRESLEKRGIDTVAQPLVAGIMPITNSSNLMRFADSCGADIPRYVRKQLADFGDDSKAIREF
GFEVVYRLCERLISEGVPAMHFYSMNKVEPNKRLIEALGLIA

Sequences:

>Translated_282_residues
MTKPAFSFEFFPAKTEQGHEKLLSTYDELNKLSPAYFSVTYGAGGSTRSRTLDIVQALCARGETDIAPHMSCIGDDKSEI
AELLEHYKSLGIKRLVALRGDLPSGQVGMGELPFALDLVKFIREHSGDHFHIEVAAYPEMHPQARSFKLDVDNLVNKYQA
GANASITQFFYNADSYLYLRESLEKRGIDTVAQPLVAGIMPITNSSNLMRFADSCGADIPRYVRKQLADFGDDSKAIREF
GFEVVYRLCERLISEGVPAMHFYSMNKVEPNKRLIEALGLIA
>Mature_281_residues
TKPAFSFEFFPAKTEQGHEKLLSTYDELNKLSPAYFSVTYGAGGSTRSRTLDIVQALCARGETDIAPHMSCIGDDKSEIA
ELLEHYKSLGIKRLVALRGDLPSGQVGMGELPFALDLVKFIREHSGDHFHIEVAAYPEMHPQARSFKLDVDNLVNKYQAG
ANASITQFFYNADSYLYLRESLEKRGIDTVAQPLVAGIMPITNSSNLMRFADSCGADIPRYVRKQLADFGDDSKAIREFG
FEVVYRLCERLISEGVPAMHFYSMNKVEPNKRLIEALGLIA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=283, Percent_Identity=33.5689045936396, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1790377, Length=274, Percent_Identity=37.5912408759124, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25147436, Length=282, Percent_Identity=31.9148936170213, Blast_Score=157, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI25147441, Length=282, Percent_Identity=31.9148936170213, Blast_Score=157, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321313, Length=288, Percent_Identity=31.5972222222222, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6325234, Length=281, Percent_Identity=31.3167259786477, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31460; Mature: 31329

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPAFSFEFFPAKTEQGHEKLLSTYDELNKLSPAYFSVTYGAGGSTRSRTLDIVQALCA
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
RGETDIAPHMSCIGDDKSEIAELLEHYKSLGIKRLVALRGDLPSGQVGMGELPFALDLVK
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
FIREHSGDHFHIEVAAYPEMHPQARSFKLDVDNLVNKYQAGANASITQFFYNADSYLYLR
HHHHCCCCEEEEEEEECCCCCCCCHHEEECHHHHHHHHHCCCCCHHHHHHHCCCCHHHHH
ESLEKRGIDTVAQPLVAGIMPITNSSNLMRFADSCGADIPRYVRKQLADFGDDSKAIREF
HHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHH
GFEVVYRLCERLISEGVPAMHFYSMNKVEPNKRLIEALGLIA
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TKPAFSFEFFPAKTEQGHEKLLSTYDELNKLSPAYFSVTYGAGGSTRSRTLDIVQALCA
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
RGETDIAPHMSCIGDDKSEIAELLEHYKSLGIKRLVALRGDLPSGQVGMGELPFALDLVK
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
FIREHSGDHFHIEVAAYPEMHPQARSFKLDVDNLVNKYQAGANASITQFFYNADSYLYLR
HHHHCCCCEEEEEEEECCCCCCCCHHEEECHHHHHHHHHCCCCCHHHHHHHCCCCHHHHH
ESLEKRGIDTVAQPLVAGIMPITNSSNLMRFADSCGADIPRYVRKQLADFGDDSKAIREF
HHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHH
GFEVVYRLCERLISEGVPAMHFYSMNKVEPNKRLIEALGLIA
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]