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The map label for this gene is gloB [H]

Identifier: 93006258

GI number: 93006258

Start: 1760362

End: 1761141

Strand: Reverse

Name: gloB [H]

Synonym: Pcryo_1432

Alternate gene names: 93006258

Gene position: 1761141-1760362 (Counterclockwise)

Preceding gene: 93006259

Following gene: 93006257

Centisome position: 57.56

GC content: 43.72

Gene sequence:

>780_bases
ATGAGTATTCGTATTCACCCAATTAAAGCATTCAATGACAACTATATTTGGACACTAATCAATGAGAGTAACAAGCAAGC
CATTGTTATTGATCCGGGTCAAGCGGCACCAGTTGTTGCTTATTTAGAGGAAAATGGATTAGAGCTGACGTCTATCTGGA
CGACTCATCATCATCATGACCATATCGGCGGCGTTGCAGAGTTACAAGAATCGTATCCGATGACGCATCTGGTTGCGCAT
ACCGAGCATAATGTCGATGAAGATCAGACCATCAAAGATGGCAGTACAGTAAGCGCGTGGGGCTGCTCTGCACAAGTTTG
GGATGTGTCAGGTCATACCGCTAGCCATATGGCTTATATATTAGATATCGATGGCCTTAAGCATTGCTTTTGTGGCGATA
CCTTATTTAGTGCTGGATGCGGACGTGTGTTTACAGGAACGATTGAGCAGTTGCATGACAGCTTTAAGCGTCTAAATGGA
CTGCCAGCTGAGACGCTGTTATATCCTGCACATGAATATACTGCTAGCAATCTACGTTTTGGGTTGTCGATTGAGCCTGA
TAATGAAGCGATGCAGCAAGCTTTGGCACAAGCAGAAGACAAAACCGCACAAGGCATTCCGACGCTCCCTGTTACTTTAG
GACATGAACGTGCGATCAATGTGTTTTTACGGACGCAAGAGCCGAGTGTGATTGCAGGTGTAAAAGCTCAGTTTAATATC
GATGATGATAAATCTTTGACTGTATTTGCAGCTCTACGTGAGCTTAAAAATAACTTTTAA

Upstream 100 bases:

>100_bases
AAAACAGTAAGTGACAGTGACAGTGACAATGACATTAAAAGTAATTTGAAAGAATTTAATAGACATTAGCAATAAAGATA
ACCACTGGTAAGGATTTAGT

Downstream 100 bases:

>100_bases
ATTCTAATCAATGCTTCGACTGACGCTTTGAGAGTTATTAGCTGCGCATTATTTCTATTGCCATATTTCTTACTATTAGG
AAGCCGCCATTATGGGTCGT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSIRIHPIKAFNDNYIWTLINESNKQAIVIDPGQAAPVVAYLEENGLELTSIWTTHHHHDHIGGVAELQESYPMTHLVAH
TEHNVDEDQTIKDGSTVSAWGCSAQVWDVSGHTASHMAYILDIDGLKHCFCGDTLFSAGCGRVFTGTIEQLHDSFKRLNG
LPAETLLYPAHEYTASNLRFGLSIEPDNEAMQQALAQAEDKTAQGIPTLPVTLGHERAINVFLRTQEPSVIAGVKAQFNI
DDDKSLTVFAALRELKNNF

Sequences:

>Translated_259_residues
MSIRIHPIKAFNDNYIWTLINESNKQAIVIDPGQAAPVVAYLEENGLELTSIWTTHHHHDHIGGVAELQESYPMTHLVAH
TEHNVDEDQTIKDGSTVSAWGCSAQVWDVSGHTASHMAYILDIDGLKHCFCGDTLFSAGCGRVFTGTIEQLHDSFKRLNG
LPAETLLYPAHEYTASNLRFGLSIEPDNEAMQQALAQAEDKTAQGIPTLPVTLGHERAINVFLRTQEPSVIAGVKAQFNI
DDDKSLTVFAALRELKNNF
>Mature_258_residues
SIRIHPIKAFNDNYIWTLINESNKQAIVIDPGQAAPVVAYLEENGLELTSIWTTHHHHDHIGGVAELQESYPMTHLVAHT
EHNVDEDQTIKDGSTVSAWGCSAQVWDVSGHTASHMAYILDIDGLKHCFCGDTLFSAGCGRVFTGTIEQLHDSFKRLNGL
PAETLLYPAHEYTASNLRFGLSIEPDNEAMQQALAQAEDKTAQGIPTLPVTLGHERAINVFLRTQEPSVIAGVKAQFNID
DDKSLTVFAALRELKNNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=268, Percent_Identity=31.7164179104478, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI94538320, Length=261, Percent_Identity=31.8007662835249, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI14150041, Length=233, Percent_Identity=32.618025751073, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI116642887, Length=262, Percent_Identity=32.0610687022901, Blast_Score=130, Evalue=8e-31,
Organism=Homo sapiens, GI21703352, Length=263, Percent_Identity=31.5589353612167, Blast_Score=130, Evalue=9e-31,
Organism=Homo sapiens, GI46361987, Length=204, Percent_Identity=30.3921568627451, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI41327741, Length=165, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1786406, Length=258, Percent_Identity=39.5348837209302, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1787158, Length=197, Percent_Identity=28.9340101522843, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=254, Percent_Identity=31.8897637795276, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21356335, Length=235, Percent_Identity=33.1914893617021, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24667703, Length=235, Percent_Identity=33.1914893617021, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24667711, Length=235, Percent_Identity=33.1914893617021, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28584; Mature: 28453

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRIHPIKAFNDNYIWTLINESNKQAIVIDPGQAAPVVAYLEENGLELTSIWTTHHHHD
CCEEEEEEEEECCCEEEEEEECCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCC
HIGGVAELQESYPMTHLVAHTEHNVDEDQTIKDGSTVSAWGCSAQVWDVSGHTASHMAYI
CCCCHHHHHHHCCCEEEEEECCCCCCCCCEECCCCEEEECCCCEEEEECCCCCHHHEEEE
LDIDGLKHCFCGDTLFSAGCGRVFTGTIEQLHDSFKRLNGLPAETLLYPAHEYTASNLRF
EECCCCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEECCHHCCCCCEEE
GLSIEPDNEAMQQALAQAEDKTAQGIPTLPVTLGHERAINVFLRTQEPSVIAGVKAQFNI
EEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEEECCCCEEEEEEEEECC
DDDKSLTVFAALRELKNNF
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SIRIHPIKAFNDNYIWTLINESNKQAIVIDPGQAAPVVAYLEENGLELTSIWTTHHHHD
CEEEEEEEEECCCEEEEEEECCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCC
HIGGVAELQESYPMTHLVAHTEHNVDEDQTIKDGSTVSAWGCSAQVWDVSGHTASHMAYI
CCCCHHHHHHHCCCEEEEEECCCCCCCCCEECCCCEEEECCCCEEEEECCCCCHHHEEEE
LDIDGLKHCFCGDTLFSAGCGRVFTGTIEQLHDSFKRLNGLPAETLLYPAHEYTASNLRF
EECCCCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEECCHHCCCCCEEE
GLSIEPDNEAMQQALAQAEDKTAQGIPTLPVTLGHERAINVFLRTQEPSVIAGVKAQFNI
EEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEEECCCCEEEEEEEEECC
DDDKSLTVFAALRELKNNF
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA