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The map label for this gene is yafC [C]

Identifier: 93006155

GI number: 93006155

Start: 1625180

End: 1626115

Strand: Reverse

Name: yafC [C]

Synonym: Pcryo_1327

Alternate gene names: 93006155

Gene position: 1626115-1625180 (Counterclockwise)

Preceding gene: 93006158

Following gene: 93006153

Centisome position: 53.14

GC content: 41.56

Gene sequence:

>936_bases
ATGAATACGGTAGATACCATCAACATACAAACACTATTATTTTTTATAGAGGTTTTTGACGCTCAGAGTTTTTCTGTAGT
GGCTCGAAAAGAAGGCGTTTCGGCTTCTAAAGTGTCACGTATTATCAAGCAGTTAGAGGACTCACTGGGACAGCAGCTTT
TTTACCGTAATACACGCGCTGTTACTCCTACTGAAGCGGGTCGTGTGTTTATGCGCTATGCAAAATCCATGACCGAGAGC
ATGAGTGCAGCACAGCAGGAGCTTCAAGACAGGACATTAGAACCGGGTGGTTTAATTAGAATCAACGCTCCCGTATTTTT
TGGACAACGACATATCGCACCTTGGCTGCCACAGCTTAGTGCTCAGTATCCTAAATTACAGATAGATTTGAGTTTAACGG
ATGACTTTATTGATCCGCATCATTATGCAACAGACGTTATTTTTAGAATTGGCACGTTACATGACTCCGCCTTTCATGCG
CGTATTTTTGGCGAGCAAACCTATCATCTTGCCGCCTCTCCATCGTACATAAGCACGCATGGTAAATTGCAAGTGCCAGC
AGATCTGACACATCATAAATGTTTACTATATAAAGGTTCAACAGGACCCAATCGTTGGCTTTTTAAAACAGAAAGTGAAG
ATTGGACGCCATTCTTAGCACCCGCTTTATTGATGTCCAATAATGCAGAATCTTTATTGGTATCTGCACTCAAGGGTATG
GGGATTGTGCTTTTTCCAGATTGGCTCATCGGTGAGCATTTAAAGAGTGGTGAGCTTATCAAGCTACTGCCTAATTATAG
TACGGCCGTCAAAACCACACCTCAACATGTCGCAGCTATTTATCCTCATACTCGTCATGTCTCTCTAAATGTTAGAACAT
TAATTGATTATTTTGCCAAAGTTTATGGTCACCCTCTTTATTGGCAACTCAATTAA

Upstream 100 bases:

>100_bases
ATGAAAAAATCCTTAATGAGCAATACGCTGCAGCTTACAGCAATGTATACCCATTAAAAAACGCTATAATACGAAAATGA
CCTTTGCATAAGTTGCACAA

Downstream 100 bases:

>100_bases
AAAACAAGAATTTAAAGCAATAAGTATCCATGCACCTTATGAGGAAAAGGTGCAATAGGAATATGTAATTTTACCCATTC
AGGAGCGTTCCTAACTCAGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNTVDTINIQTLLFFIEVFDAQSFSVVARKEGVSASKVSRIIKQLEDSLGQQLFYRNTRAVTPTEAGRVFMRYAKSMTES
MSAAQQELQDRTLEPGGLIRINAPVFFGQRHIAPWLPQLSAQYPKLQIDLSLTDDFIDPHHYATDVIFRIGTLHDSAFHA
RIFGEQTYHLAASPSYISTHGKLQVPADLTHHKCLLYKGSTGPNRWLFKTESEDWTPFLAPALLMSNNAESLLVSALKGM
GIVLFPDWLIGEHLKSGELIKLLPNYSTAVKTTPQHVAAIYPHTRHVSLNVRTLIDYFAKVYGHPLYWQLN

Sequences:

>Translated_311_residues
MNTVDTINIQTLLFFIEVFDAQSFSVVARKEGVSASKVSRIIKQLEDSLGQQLFYRNTRAVTPTEAGRVFMRYAKSMTES
MSAAQQELQDRTLEPGGLIRINAPVFFGQRHIAPWLPQLSAQYPKLQIDLSLTDDFIDPHHYATDVIFRIGTLHDSAFHA
RIFGEQTYHLAASPSYISTHGKLQVPADLTHHKCLLYKGSTGPNRWLFKTESEDWTPFLAPALLMSNNAESLLVSALKGM
GIVLFPDWLIGEHLKSGELIKLLPNYSTAVKTTPQHVAAIYPHTRHVSLNVRTLIDYFAKVYGHPLYWQLN
>Mature_311_residues
MNTVDTINIQTLLFFIEVFDAQSFSVVARKEGVSASKVSRIIKQLEDSLGQQLFYRNTRAVTPTEAGRVFMRYAKSMTES
MSAAQQELQDRTLEPGGLIRINAPVFFGQRHIAPWLPQLSAQYPKLQIDLSLTDDFIDPHHYATDVIFRIGTLHDSAFHA
RIFGEQTYHLAASPSYISTHGKLQVPADLTHHKCLLYKGSTGPNRWLFKTESEDWTPFLAPALLMSNNAESLLVSALKGM
GIVLFPDWLIGEHLKSGELIKLLPNYSTAVKTTPQHVAAIYPHTRHVSLNVRTLIDYFAKVYGHPLYWQLN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=31.3993174061433, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=30.1694915254237, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=29.0969899665552, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789440, Length=293, Percent_Identity=27.9863481228669, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081978, Length=308, Percent_Identity=31.4935064935065, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=26.6211604095563, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=25.3424657534247, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788706, Length=249, Percent_Identity=22.8915662650602, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790208, Length=215, Percent_Identity=25.1162790697674, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35111; Mature: 35111

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVDTINIQTLLFFIEVFDAQSFSVVARKEGVSASKVSRIIKQLEDSLGQQLFYRNTRA
CCCCCEEHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VTPTEAGRVFMRYAKSMTESMSAAQQELQDRTLEPGGLIRINAPVFFGQRHIAPWLPQLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCCCCCCCHHHC
AQYPKLQIDLSLTDDFIDPHHYATDVIFRIGTLHDSAFHARIFGEQTYHLAASPSYISTH
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCEEECC
GKLQVPADLTHHKCLLYKGSTGPNRWLFKTESEDWTPFLAPALLMSNNAESLLVSALKGM
CCEECCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCC
GIVLFPDWLIGEHLKSGELIKLLPNYSTAVKTTPQHVAAIYPHTRHVSLNVRTLIDYFAK
EEEEECCHHHHHHCCCCCEEEECCCCCCCCCCCHHHHEEECCCCEEEEEHHHHHHHHHHH
VYGHPLYWQLN
HHCCCEEEEEC
>Mature Secondary Structure
MNTVDTINIQTLLFFIEVFDAQSFSVVARKEGVSASKVSRIIKQLEDSLGQQLFYRNTRA
CCCCCEEHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VTPTEAGRVFMRYAKSMTESMSAAQQELQDRTLEPGGLIRINAPVFFGQRHIAPWLPQLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCCCCCCCHHHC
AQYPKLQIDLSLTDDFIDPHHYATDVIFRIGTLHDSAFHARIFGEQTYHLAASPSYISTH
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCEEECC
GKLQVPADLTHHKCLLYKGSTGPNRWLFKTESEDWTPFLAPALLMSNNAESLLVSALKGM
CCEECCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCC
GIVLFPDWLIGEHLKSGELIKLLPNYSTAVKTTPQHVAAIYPHTRHVSLNVRTLIDYFAK
EEEEECCHHHHHHCCCCCEEEECCCCCCCCCCCHHHHEEECCCCEEEEEHHHHHHHHHHH
VYGHPLYWQLN
HHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]