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The map label for this gene is yoeB [H]

Identifier: 93006135

GI number: 93006135

Start: 1596414

End: 1596674

Strand: Reverse

Name: yoeB [H]

Synonym: Pcryo_1307

Alternate gene names: 93006135

Gene position: 1596674-1596414 (Counterclockwise)

Preceding gene: 93006136

Following gene: 93006134

Centisome position: 52.18

GC content: 41.0

Gene sequence:

>261_bases
ATGAGTGAGCAACTAGCATGGACAGATGCTGCGTGGAAAGACTATCTGTACTGGCAAACACAAGACAAAAAAACACTCAA
ACGTGTTAACAAACTCATCACCGAATGTAAGCGTAATCCGTTTGAAGGCATTGGTAAACCTGAACCCCTAAAAGAAAATC
TATCCGGATTTTGGTCAAGACGCATCGATGATGCCAATCGGTTGGTATATGCCGTCGATGATAACTATCTGACGATTATC
TCTTCTCGCTATCATTATTAA

Upstream 100 bases:

>100_bases
GTGATAAAAGTGATGGTAGCAGCGATGACAATGAGCATACGAATGGCGACGTTGAGAATGCTTGAACTCAAAGGCTTTAA
TCAATGCAAAGGGTCTAATA

Downstream 100 bases:

>100_bases
CGAGCGCTCTTAGTCGTTCGTGTTTACTTGCTGATGGCACCATACCCAATCTTATAACAATAGAAAAAGGACTTAATACA
GTGGCCCAAACCAATTCTAA

Product: addiction module toxin, Txe/YoeB

Products: NA

Alternate protein names: Putative endoribonuclease YoeB; Putative mRNA interferase Yoeb [H]

Number of amino acids: Translated: 86; Mature: 85

Protein sequence:

>86_residues
MSEQLAWTDAAWKDYLYWQTQDKKTLKRVNKLITECKRNPFEGIGKPEPLKENLSGFWSRRIDDANRLVYAVDDNYLTII
SSRYHY

Sequences:

>Translated_86_residues
MSEQLAWTDAAWKDYLYWQTQDKKTLKRVNKLITECKRNPFEGIGKPEPLKENLSGFWSRRIDDANRLVYAVDDNYLTII
SSRYHY
>Mature_85_residues
SEQLAWTDAAWKDYLYWQTQDKKTLKRVNKLITECKRNPFEGIGKPEPLKENLSGFWSRRIDDANRLVYAVDDNYLTIIS
SRYHY

Specific function: Part of a toxin-antitoxin (TA) module. It has been proposed to be an mRNA interferase but also an inhibitor of translation initiation. Has an in vitro RNase activity and preferentially cleaves at the 3'-end of purine ribonucleotides. YefM binds to the pro

COG id: COG4115

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YoeB family [H]

Homologues:

Organism=Escherichia coli, GI87082025, Length=83, Percent_Identity=60.2409638554217, Blast_Score=114, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009614 [H]

Pfam domain/function: PF06769 Plasmid_Txe [H]

EC number: NA

Molecular weight: Translated: 10304; Mature: 10173

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLAWTDAAWKDYLYWQTQDKKTLKRVNKLITECKRNPFEGIGKPEPLKENLSGFWSR
CCCCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
RIDDANRLVYAVDDNYLTIISSRYHY
CCCCCCEEEEEECCCEEEEECCCCCC
>Mature Secondary Structure 
SEQLAWTDAAWKDYLYWQTQDKKTLKRVNKLITECKRNPFEGIGKPEPLKENLSGFWSR
CCCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
RIDDANRLVYAVDDNYLTIISSRYHY
CCCCCCEEEEEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]