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The map label for this gene is mii [H]

Identifier: 93006134

GI number: 93006134

Start: 1595092

End: 1596333

Strand: Reverse

Name: mii [H]

Synonym: Pcryo_1306

Alternate gene names: 93006134

Gene position: 1596333-1595092 (Counterclockwise)

Preceding gene: 93006135

Following gene: 93006133

Centisome position: 52.17

GC content: 47.18

Gene sequence:

>1242_bases
GTGGCCCAAACCAATTCTAAATCAAAACCCTCTTTCGCCCCCCAACTATCCGTTCCTGCTACCTATATGCGCGGCGGTAC
CTCCAAAGGCACGTTTTTTAAATTATCTGATTTACCTGAGCGCTGCCAAGTCGCTGGTGAATCACGTGATCAATTTTTGC
TACGTGTCATCGGTAGCCCAGATCCTTATGGTAAGCAAATCGACGGTTTAGGCAATGGCAGCTCTAGCACCTCTAAGACA
GTAATATTGTCTGAATCTGATAAAGCAGACCACGATGTCAACTATTTGTTTGGACAGGTCAATATTGCCAAGCCTATGAT
TGATTGGGCGGGTAACTGTGGCAACTTAACCGCTTCAGTTGGCGCTTGTGCGATTAATATGGGTCTGATCGATGCTGATA
AGATCGCCAATAGTGCGAATACTGGCATCTGTGAAGTGCATATTTGGCAAGAGAATATCAGTAAAACCATTATCGCTCAC
GTGCCTGTGTATCAAGATGAGCAAGGCAAGGTACAAGTGCAAGAAACCGGTGACTTTGAATTAGACGGTGTGACCTTCCC
AGCCGCTGAAGTCAAAATTGAGTTCATCGATCCAGTTGATTCATCTAGTGACATGTTCCCGACAAACAATCTAATCGATG
ACTTCGATGTCTCAGGCTGTGGTCTAAATATTGATAGTGTCAAAGCAACCTTTATCAGTGCCGGTATTCCAACTATTTTT
ATGAAAGCAGAAGATTTAGGCTTTACCGGTACTGAGCTGCAAGGCGACATCAATAGCGATGGTGAGCTACTTGCCAAGCT
TGAGAAAATCCGTGCCACAGGCGGTGTGGCGATGGGATTATTTAAAGACGTTTCTGAAGCACAGACCAGCCAGCACATTC
CAAAAATCGCATGGGTAGCTCCTGCGCAAAGTTACAGCGCCTCAAGTGGCAAAGCCGTTGATGCCAATGATATTGATTTG
GTCGTACGGGCGATGAGTATGGGTCAATTGCATCATGCGATGATGGGTACGGCCGCGGTTGCTATTGCTGCGGCAGCGAC
GACGCAAGGGACATTGGTTAATGAAGCAGCAGGCGGTAGCGAGTTAGGTGAAGTTCGTTTTGGTCATCCCTCTGGCACCT
TGCTCGTCGGCGGAAAAACCGAACTGATCGATGGACGCTGGCAAGCCAAAAAAGTCTCGATGAGTCGCTCAGCACGTCGT
ATCATGATAGGTGAAGTGTTTGTGCCAGCAGATGCGTTTTAA

Upstream 100 bases:

>100_bases
CTTCTCGCTATCATTATTAACGAGCGCTCTTAGTCGTTCGTGTTTACTTGCTGATGGCACCATACCCAATCTTATAACAA
TAGAAAAAGGACTTAATACA

Downstream 100 bases:

>100_bases
TTTTGATGATATTTTTCTCTGATATAATTTGAAGCCCAAATAGCTAGATTAATAAAATAAAGGCTAAACTCATATGTTAT
TTAATGAAGTCATTGAACGG

Product: PrpF, AcnD-accessory

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MAQTNSKSKPSFAPQLSVPATYMRGGTSKGTFFKLSDLPERCQVAGESRDQFLLRVIGSPDPYGKQIDGLGNGSSSTSKT
VILSESDKADHDVNYLFGQVNIAKPMIDWAGNCGNLTASVGACAINMGLIDADKIANSANTGICEVHIWQENISKTIIAH
VPVYQDEQGKVQVQETGDFELDGVTFPAAEVKIEFIDPVDSSSDMFPTNNLIDDFDVSGCGLNIDSVKATFISAGIPTIF
MKAEDLGFTGTELQGDINSDGELLAKLEKIRATGGVAMGLFKDVSEAQTSQHIPKIAWVAPAQSYSASSGKAVDANDIDL
VVRAMSMGQLHHAMMGTAAVAIAAAATTQGTLVNEAAGGSELGEVRFGHPSGTLLVGGKTELIDGRWQAKKVSMSRSARR
IMIGEVFVPADAF

Sequences:

>Translated_413_residues
MAQTNSKSKPSFAPQLSVPATYMRGGTSKGTFFKLSDLPERCQVAGESRDQFLLRVIGSPDPYGKQIDGLGNGSSSTSKT
VILSESDKADHDVNYLFGQVNIAKPMIDWAGNCGNLTASVGACAINMGLIDADKIANSANTGICEVHIWQENISKTIIAH
VPVYQDEQGKVQVQETGDFELDGVTFPAAEVKIEFIDPVDSSSDMFPTNNLIDDFDVSGCGLNIDSVKATFISAGIPTIF
MKAEDLGFTGTELQGDINSDGELLAKLEKIRATGGVAMGLFKDVSEAQTSQHIPKIAWVAPAQSYSASSGKAVDANDIDL
VVRAMSMGQLHHAMMGTAAVAIAAAATTQGTLVNEAAGGSELGEVRFGHPSGTLLVGGKTELIDGRWQAKKVSMSRSARR
IMIGEVFVPADAF
>Mature_412_residues
AQTNSKSKPSFAPQLSVPATYMRGGTSKGTFFKLSDLPERCQVAGESRDQFLLRVIGSPDPYGKQIDGLGNGSSSTSKTV
ILSESDKADHDVNYLFGQVNIAKPMIDWAGNCGNLTASVGACAINMGLIDADKIANSANTGICEVHIWQENISKTIIAHV
PVYQDEQGKVQVQETGDFELDGVTFPAAEVKIEFIDPVDSSSDMFPTNNLIDDFDVSGCGLNIDSVKATFISAGIPTIFM
KAEDLGFTGTELQGDINSDGELLAKLEKIRATGGVAMGLFKDVSEAQTSQHIPKIAWVAPAQSYSASSGKAVDANDIDLV
VRAMSMGQLHHAMMGTAAVAIAAAATTQGTLVNEAAGGSELGEVRFGHPSGTLLVGGKTELIDGRWQAKKVSMSRSARRI
MIGEVFVPADAF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=393, Percent_Identity=34.6055979643766, Blast_Score=181, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 43632; Mature: 43500

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTNSKSKPSFAPQLSVPATYMRGGTSKGTFFKLSDLPERCQVAGESRDQFLLRVIGSP
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCHHHHHCCCCCCEEEEEEECCC
DPYGKQIDGLGNGSSSTSKTVILSESDKADHDVNYLFGQVNIAKPMIDWAGNCGNLTASV
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEEECCHHHHHCCCCCCEEECC
GACAINMGLIDADKIANSANTGICEVHIWQENISKTIIAHVPVYQDEQGKVQVQETGDFE
CEEEEEECCEEHHHHCCCCCCCEEEEEEECCCCCCEEEEECCCEECCCCCEEEEECCCEE
LDGVTFPAAEVKIEFIDPVDSSSDMFPTNNLIDDFDVSGCGLNIDSVKATFISAGIPTIF
ECCEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEECCCCCEEE
MKAEDLGFTGTELQGDINSDGELLAKLEKIRATGGVAMGLFKDVSEAQTSQHIPKIAWVA
EEECCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEC
PAQSYSASSGKAVDANDIDLVVRAMSMGQLHHAMMGTAAVAIAAAATTQGTLVNEAAGGS
CCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
ELGEVRFGHPSGTLLVGGKTELIDGRWQAKKVSMSRSARRIMIGEVFVPADAF
CCCCEEECCCCCEEEECCCEEEECCCCCHHHHHHHHCCCEEEEEEEEECCCCC
>Mature Secondary Structure 
AQTNSKSKPSFAPQLSVPATYMRGGTSKGTFFKLSDLPERCQVAGESRDQFLLRVIGSP
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCHHHHHCCCCCCEEEEEEECCC
DPYGKQIDGLGNGSSSTSKTVILSESDKADHDVNYLFGQVNIAKPMIDWAGNCGNLTASV
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEEECCHHHHHCCCCCCEEECC
GACAINMGLIDADKIANSANTGICEVHIWQENISKTIIAHVPVYQDEQGKVQVQETGDFE
CEEEEEECCEEHHHHCCCCCCCEEEEEEECCCCCCEEEEECCCEECCCCCEEEEECCCEE
LDGVTFPAAEVKIEFIDPVDSSSDMFPTNNLIDDFDVSGCGLNIDSVKATFISAGIPTIF
ECCEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEECCCCCEEE
MKAEDLGFTGTELQGDINSDGELLAKLEKIRATGGVAMGLFKDVSEAQTSQHIPKIAWVA
EEECCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEC
PAQSYSASSGKAVDANDIDLVVRAMSMGQLHHAMMGTAAVAIAAAATTQGTLVNEAAGGS
CCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
ELGEVRFGHPSGTLLVGGKTELIDGRWQAKKVSMSRSARRIMIGEVFVPADAF
CCCCEEECCCCCEEEECCCEEEECCCCCHHHHHHHHCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA