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The map label for this gene is sotB [H]

Identifier: 93005696

GI number: 93005696

Start: 1027462

End: 1028661

Strand: Reverse

Name: sotB [H]

Synonym: Pcryo_0868

Alternate gene names: 93005696

Gene position: 1028661-1027462 (Counterclockwise)

Preceding gene: 93005705

Following gene: 93005694

Centisome position: 33.62

GC content: 49.58

Gene sequence:

>1200_bases
ATGAAACCTAACATTGAGGTGAGCGCAAATGCTCGCCGGACTCAGTATTTTCAAGTATTCCTAATGGGCGTCAGCGCCTT
CATTATGAATACCACCGAATTCGTCCCTGTTGCCCTATTAAGCGACATCGCCCAAGATTTCTCTATCACCACGGCAGAGA
CGGGCTGGATGTTGACGCTGTATGCGTGGATTGTCGCGGCCATGTCATTGCCATTGATGCTGCTGACCAGTCGCTTTGAG
CGCAAGCGTTTGCTGTTGGCCTTGTTTGCGGTGTTTATTGCCAGCCATGTGTTGTCAGTATTTGCGTGGAGCTTTGAAGT
GCTGCTCATCAGCCGAGTTGGCATTGCGCTCACGCATGCGATATTTTGGTCCATCACCGCAGCGATTGCGATACGTGTGG
CACCGGAAGGCAAAAAAGCACTGGCGCTTAGCGTGCTTGCGACCGGTACGTCATTGGCGATGGTGTTGGGTGTGCCTTTA
GGACGCGTGGTCGGTCAATGGTTTGGCTGGCGCGCGACTTTTGGTGGTATTGGTGGGCTTGCCCTTATCGTATTTATTCT
GCAAATGAGACTGTTGCCCACGCTGCCTAGTATGTTTGAAGGGTCTTTTAGAAAGATTCCAGAATTACTCAAAAACCCCT
TATTGGTCTGCCTATATCTGCTAATTTTACTGGTCTTTACCGCGCACTATACGGCTTACTCGTATATCGAGCCTTTTATG
CGTCAAGTCGGCTCAATCAGTGAAAACGCCGCAACGGTTGTGCTGCTATTGTTCGGTGTCGCAGGGATTGCGGGCAGTGT
AATTTTCAGTCGTTGGGGTGATAAATTCAATACCAGATTAATGCTGATATCGACGATACTGATACTATTGTCTATGCTCA
TGCTGTTATTTGCGGTGACCTCAATATGGGCGCTCAGCTTGATTGCCCTACTCTGGGGCGCGGCGCTCATGCTGCTTATC
ATCTCCATGCAAGCCAAGGTCATCATGGTCGATGTCAATGCACAGGATATGCTGATGTCGATGTTTTCAGGGATTATCAA
TTTAGGCATTGGGACGGGCGCACTGTTCGGAGGCTATGCAGTGACGCATATTTCACTATCGAGCGTGGGATATGTAGGGG
CGGCTATCGCGGTTATGGCATTGCTACTGATGCTATTTATGATAAAGCGCTTTCCTGAGCTAAGCAGAAGGCTCGGCTAG

Upstream 100 bases:

>100_bases
GCCATTGACTTGCTATCTTGCTTACCTTTTCTATGAACATCTCACTTTCTGCCCCGTTTTTACGCTAATTTTTTAGCATT
CTTTCTGACCGTTGGATCTT

Downstream 100 bases:

>100_bases
CTGAAAAGCTGATAGCAAATAAGTATATAAAAAATGCCAGCCACTTAGATGGGCTGGCATTTTGCTTTTTGGTTAATTAT
GAAAATCCACCTTCAAATCA

Product: sugar efflux transporter

Products: arabinose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKPNIEVSANARRTQYFQVFLMGVSAFIMNTTEFVPVALLSDIAQDFSITTAETGWMLTLYAWIVAAMSLPLMLLTSRFE
RKRLLLALFAVFIASHVLSVFAWSFEVLLISRVGIALTHAIFWSITAAIAIRVAPEGKKALALSVLATGTSLAMVLGVPL
GRVVGQWFGWRATFGGIGGLALIVFILQMRLLPTLPSMFEGSFRKIPELLKNPLLVCLYLLILLVFTAHYTAYSYIEPFM
RQVGSISENAATVVLLLFGVAGIAGSVIFSRWGDKFNTRLMLISTILILLSMLMLLFAVTSIWALSLIALLWGAALMLLI
ISMQAKVIMVDVNAQDMLMSMFSGIINLGIGTGALFGGYAVTHISLSSVGYVGAAIAVMALLLMLFMIKRFPELSRRLG

Sequences:

>Translated_399_residues
MKPNIEVSANARRTQYFQVFLMGVSAFIMNTTEFVPVALLSDIAQDFSITTAETGWMLTLYAWIVAAMSLPLMLLTSRFE
RKRLLLALFAVFIASHVLSVFAWSFEVLLISRVGIALTHAIFWSITAAIAIRVAPEGKKALALSVLATGTSLAMVLGVPL
GRVVGQWFGWRATFGGIGGLALIVFILQMRLLPTLPSMFEGSFRKIPELLKNPLLVCLYLLILLVFTAHYTAYSYIEPFM
RQVGSISENAATVVLLLFGVAGIAGSVIFSRWGDKFNTRLMLISTILILLSMLMLLFAVTSIWALSLIALLWGAALMLLI
ISMQAKVIMVDVNAQDMLMSMFSGIINLGIGTGALFGGYAVTHISLSSVGYVGAAIAVMALLLMLFMIKRFPELSRRLG
>Mature_399_residues
MKPNIEVSANARRTQYFQVFLMGVSAFIMNTTEFVPVALLSDIAQDFSITTAETGWMLTLYAWIVAAMSLPLMLLTSRFE
RKRLLLALFAVFIASHVLSVFAWSFEVLLISRVGIALTHAIFWSITAAIAIRVAPEGKKALALSVLATGTSLAMVLGVPL
GRVVGQWFGWRATFGGIGGLALIVFILQMRLLPTLPSMFEGSFRKIPELLKNPLLVCLYLLILLVFTAHYTAYSYIEPFM
RQVGSISENAATVVLLLFGVAGIAGSVIFSRWGDKFNTRLMLISTILILLSMLMLLFAVTSIWALSLIALLWGAALMLLI
ISMQAKVIMVDVNAQDMLMSMFSGIINLGIGTGALFGGYAVTHISLSSVGYVGAAIAVMALLLMLFMIKRFPELSRRLG

Specific function: Involved in the efflux of sugars. The physiological role may be the reduction of the intracellular concentration of toxic sugars or sugar metabolites [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. SotB (TC 2.A.1.2) family [H]

Homologues:

Organism=Escherichia coli, GI1787808, Length=382, Percent_Identity=53.4031413612565, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI145693198, Length=358, Percent_Identity=28.2122905027933, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787947, Length=384, Percent_Identity=27.0833333333333, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786595, Length=341, Percent_Identity=25.8064516129032, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43581; Mature: 43581

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNIEVSANARRTQYFQVFLMGVSAFIMNTTEFVPVALLSDIAQDFSITTAETGWMLTL
CCCCCEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCHHHHH
YAWIVAAMSLPLMLLTSRFERKRLLLALFAVFIASHVLSVFAWSFEVLLISRVGIALTHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFWSITAAIAIRVAPEGKKALALSVLATGTSLAMVLGVPLGRVVGQWFGWRATFGGIGGL
HHHHHHHHHHEEECCCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCHHHHCCHHHH
ALIVFILQMRLLPTLPSMFEGSFRKIPELLKNPLLVCLYLLILLVFTAHYTAYSYIEPFM
HHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQVGSISENAATVVLLLFGVAGIAGSVIFSRWGDKFNTRLMLISTILILLSMLMLLFAVT
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SIWALSLIALLWGAALMLLIISMQAKVIMVDVNAQDMLMSMFSGIINLGIGTGALFGGYA
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHH
VTHISLSSVGYVGAAIAVMALLLMLFMIKRFPELSRRLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
>Mature Secondary Structure
MKPNIEVSANARRTQYFQVFLMGVSAFIMNTTEFVPVALLSDIAQDFSITTAETGWMLTL
CCCCCEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCHHHHH
YAWIVAAMSLPLMLLTSRFERKRLLLALFAVFIASHVLSVFAWSFEVLLISRVGIALTHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFWSITAAIAIRVAPEGKKALALSVLATGTSLAMVLGVPLGRVVGQWFGWRATFGGIGGL
HHHHHHHHHHEEECCCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCHHHHCCHHHH
ALIVFILQMRLLPTLPSMFEGSFRKIPELLKNPLLVCLYLLILLVFTAHYTAYSYIEPFM
HHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQVGSISENAATVVLLLFGVAGIAGSVIFSRWGDKFNTRLMLISTILILLSMLMLLFAVT
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SIWALSLIALLWGAALMLLIISMQAKVIMVDVNAQDMLMSMFSGIINLGIGTGALFGGYA
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHH
VTHISLSSVGYVGAAIAVMALLLMLFMIKRFPELSRRLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: arabinose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: arabinose [Cytoplasm] + Proton [Periplasm] = arabinose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA