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The map label for this gene is proS [H]

Identifier: 93005293

GI number: 93005293

Start: 566898

End: 568616

Strand: Reverse

Name: proS [H]

Synonym: Pcryo_0463

Alternate gene names: 93005293

Gene position: 568616-566898 (Counterclockwise)

Preceding gene: 93005295

Following gene: 93005287

Centisome position: 18.58

GC content: 46.25

Gene sequence:

>1719_bases
ATGAAAGCCAGTCAATTTTTATTTGCCACGCTAAAAGAAACACCCAGTGATGCAGATATCGCTTCAAGCCAATTAATGGT
TAGAGCCGGTCTGATTCGCAAAATCGCTTCTGGATTATATATCTGGTTGCCAATGGGTTTGCGCGTATTGCAAAAAGTCG
AGCGTATCGTTCGCGAAGAGATGCAAAATATCGGCGCTCAAGAAGTGCTTATGCCGATGACTCAGCCTGCTGAACTTTGG
CAGCTGACCGGACGTTTTAACGACTATGGTCCTGAGCTACTACGCTTTAAAGACCGTCATGACCGCGATTTTGTATTGGG
ACCGACGCACGAAGAAGTCATCACCAATCTTGCCCAAGGCGAGCTGCGCAGTTATAAGCAATTACCGATTACTTTTTTTC
AAATTCAAAATAAGTTCCGTGATGAGATTCGTCCACGCTTTGGTGTGATGCGCGCACGCGAATTTACCATGAAAGATGCT
TATTCCTTCCACGTTGACCAAGCATCACTGGCAAAGACGTATGATGAGATGTATGACGCTTATACGCGCATATTCACGCG
CTTAGGTCTTGATTTCCGTGCTGTACAAGCAGATACTGGCTCAATCGGCGGCTTTGCCTCACACGAGTTTCATGTCCTAG
CAGACAGCGGCGAAGATGATATTGCTTTCTCTGATTCGTCTGAGTATGCTGCCAACGTCGAGCTTGCAGAATCTGTGAGT
ATGGCAGAACGCCAGCCTGCAACGATGGCTCGCGAGAATATTGATACCGTCAATATGCCAACCTGTGAAGCCGTTGCTGA
GCACTTAAACGTTCCGCTTGCTACAACTGTCAAAACATTGATTGTGCAAGGTCATACACCAGAAGGTGAGCCACAGCTTA
TCGCTGTGGTATTACGCGGCGACCATACACTAAACACCATAAAAGCCGAAAAAATTGAAGAAGCCAATGTCCCTTTAATG
ATGGCAACGGAAGAAGAGCTAAAAGCCGCTGGTCTGCATAAAGGCTATATCGGCGTTGAGCTTAATATGCCCGTCTTTGT
AGATCGTGCAGCAGCGGCATTGTCCGACTTTGTCTCTGGCGCCAATGAAATTAACAAGCACACGACAGGTATGAATTGGG
AGCGTGACGCGCATATCACCCGTATTGTTGATATTCGCAATGTACACGAAGGCGATCCTTCTCCTGATGGTAAAGGTACG
CTACAGATCAAACGTGGTATCGAAGTCGGTCATATCTTCCAGTTGGGTGATAAGTACTCACAAGCGATGAACTGCACGGT
ATCAGGCGAAGATGGCAAGCCGGTCACTTTGATGATGGGTTGTTACGGTATTGGCGTCAGCCGTATTATCGCTGCTGCTA
TTGAGCAAAACAACGATGAAAACGGCATTATGTGGCCATCAACACCAGATATTAGTGATTCTTTAGCACCTTTTGAAGTC
GCTATCGTGCCAATGAAATCAAAAGAAGACACCGTTATGGAAACAGCAACGGCATTGTACGAGGAGCTAAAAGCTCTTGG
TATCAATGTATTGCTTGATGACCGTAATGAACGCCCAGGCGTGAAGTTTGCTGACCTTGAGCTGATTGGGATTCCGCATC
GTATCGTAGTATCTGACCGTAACTTGGCAGAAGACAAGTACGAATACGTTAATCGCCGTGATAGTGAAAAGCAACTACTT
AGCCGTGATGAAGTAATTGCTAAAGTCAGCGGTAAATAG

Upstream 100 bases:

>100_bases
CCAAGTAATAACAGGTAATCTTGCGTCGGCTATCAAACCACACGCTCAACATCCTACTATTTCTTATTTATTTTATCGTC
GATAAGGTTCCGCTTTATTT

Downstream 100 bases:

>100_bases
TGCAACGATAAAATTAGTACCATAAAAAGCGCCTATCTCAGTATATGAGATAGGCGCTTTTTTATTCTTAGAGGTTTATT
TAAAAACCATTCATAATGCT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKASQFLFATLKETPSDADIASSQLMVRAGLIRKIASGLYIWLPMGLRVLQKVERIVREEMQNIGAQEVLMPMTQPAELW
QLTGRFNDYGPELLRFKDRHDRDFVLGPTHEEVITNLAQGELRSYKQLPITFFQIQNKFRDEIRPRFGVMRAREFTMKDA
YSFHVDQASLAKTYDEMYDAYTRIFTRLGLDFRAVQADTGSIGGFASHEFHVLADSGEDDIAFSDSSEYAANVELAESVS
MAERQPATMARENIDTVNMPTCEAVAEHLNVPLATTVKTLIVQGHTPEGEPQLIAVVLRGDHTLNTIKAEKIEEANVPLM
MATEEELKAAGLHKGYIGVELNMPVFVDRAAAALSDFVSGANEINKHTTGMNWERDAHITRIVDIRNVHEGDPSPDGKGT
LQIKRGIEVGHIFQLGDKYSQAMNCTVSGEDGKPVTLMMGCYGIGVSRIIAAAIEQNNDENGIMWPSTPDISDSLAPFEV
AIVPMKSKEDTVMETATALYEELKALGINVLLDDRNERPGVKFADLELIGIPHRIVVSDRNLAEDKYEYVNRRDSEKQLL
SRDEVIAKVSGK

Sequences:

>Translated_572_residues
MKASQFLFATLKETPSDADIASSQLMVRAGLIRKIASGLYIWLPMGLRVLQKVERIVREEMQNIGAQEVLMPMTQPAELW
QLTGRFNDYGPELLRFKDRHDRDFVLGPTHEEVITNLAQGELRSYKQLPITFFQIQNKFRDEIRPRFGVMRAREFTMKDA
YSFHVDQASLAKTYDEMYDAYTRIFTRLGLDFRAVQADTGSIGGFASHEFHVLADSGEDDIAFSDSSEYAANVELAESVS
MAERQPATMARENIDTVNMPTCEAVAEHLNVPLATTVKTLIVQGHTPEGEPQLIAVVLRGDHTLNTIKAEKIEEANVPLM
MATEEELKAAGLHKGYIGVELNMPVFVDRAAAALSDFVSGANEINKHTTGMNWERDAHITRIVDIRNVHEGDPSPDGKGT
LQIKRGIEVGHIFQLGDKYSQAMNCTVSGEDGKPVTLMMGCYGIGVSRIIAAAIEQNNDENGIMWPSTPDISDSLAPFEV
AIVPMKSKEDTVMETATALYEELKALGINVLLDDRNERPGVKFADLELIGIPHRIVVSDRNLAEDKYEYVNRRDSEKQLL
SRDEVIAKVSGK
>Mature_572_residues
MKASQFLFATLKETPSDADIASSQLMVRAGLIRKIASGLYIWLPMGLRVLQKVERIVREEMQNIGAQEVLMPMTQPAELW
QLTGRFNDYGPELLRFKDRHDRDFVLGPTHEEVITNLAQGELRSYKQLPITFFQIQNKFRDEIRPRFGVMRAREFTMKDA
YSFHVDQASLAKTYDEMYDAYTRIFTRLGLDFRAVQADTGSIGGFASHEFHVLADSGEDDIAFSDSSEYAANVELAESVS
MAERQPATMARENIDTVNMPTCEAVAEHLNVPLATTVKTLIVQGHTPEGEPQLIAVVLRGDHTLNTIKAEKIEEANVPLM
MATEEELKAAGLHKGYIGVELNMPVFVDRAAAALSDFVSGANEINKHTTGMNWERDAHITRIVDIRNVHEGDPSPDGKGT
LQIKRGIEVGHIFQLGDKYSQAMNCTVSGEDGKPVTLMMGCYGIGVSRIIAAAIEQNNDENGIMWPSTPDISDSLAPFEV
AIVPMKSKEDTVMETATALYEELKALGINVLLDDRNERPGVKFADLELIGIPHRIVVSDRNLAEDKYEYVNRRDSEKQLL
SRDEVIAKVSGK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=46.9026548672566, Blast_Score=224, Evalue=2e-58,
Organism=Escherichia coli, GI1786392, Length=572, Percent_Identity=61.1888111888112, Blast_Score=706, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=208, Percent_Identity=41.3461538461538, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI193203271, Length=106, Percent_Identity=37.7358490566038, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=219, Percent_Identity=38.8127853881278, Blast_Score=170, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6321808, Length=191, Percent_Identity=28.7958115183246, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24656200, Length=246, Percent_Identity=37.3983739837398, Blast_Score=179, Evalue=6e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63766; Mature: 63766

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASQFLFATLKETPSDADIASSQLMVRAGLIRKIASGLYIWLPMGLRVLQKVERIVREE
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHH
MQNIGAQEVLMPMTQPAELWQLTGRFNDYGPELLRFKDRHDRDFVLGPTHEEVITNLAQG
HHCCCHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHCCCCCCCCEEECCCHHHHHHHHHHH
ELRSYKQLPITFFQIQNKFRDEIRPRFGVMRAREFTMKDAYSFHVDQASLAKTYDEMYDA
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHH
YTRIFTRLGLDFRAVQADTGSIGGFASHEFHVLADSGEDDIAFSDSSEYAANVELAESVS
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCEEECCCCCCEECHHHHHHHH
MAERQPATMARENIDTVNMPTCEAVAEHLNVPLATTVKTLIVQGHTPEGEPQLIAVVLRG
HHHCCCHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHEEECCCCCCCCEEEEEEEEC
DHTLNTIKAEKIEEANVPLMMATEEELKAAGLHKGYIGVELNMPVFVDRAAAALSDFVSG
CCCCCHHHHHHHHHCCCCEEEECHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
ANEINKHTTGMNWERDAHITRIVDIRNVHEGDPSPDGKGTLQIKRGIEVGHIFQLGDKYS
HHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHH
QAMNCTVSGEDGKPVTLMMGCYGIGVSRIIAAAIEQNNDENGIMWPSTPDISDSLAPFEV
HHEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEE
AIVPMKSKEDTVMETATALYEELKALGINVLLDDRNERPGVKFADLELIGIPHRIVVSDR
EEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEECCCEEEEECCC
NLAEDKYEYVNRRDSEKQLLSRDEVIAKVSGK
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKASQFLFATLKETPSDADIASSQLMVRAGLIRKIASGLYIWLPMGLRVLQKVERIVREE
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHH
MQNIGAQEVLMPMTQPAELWQLTGRFNDYGPELLRFKDRHDRDFVLGPTHEEVITNLAQG
HHCCCHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHCCCCCCCCEEECCCHHHHHHHHHHH
ELRSYKQLPITFFQIQNKFRDEIRPRFGVMRAREFTMKDAYSFHVDQASLAKTYDEMYDA
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHH
YTRIFTRLGLDFRAVQADTGSIGGFASHEFHVLADSGEDDIAFSDSSEYAANVELAESVS
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCEEECCCCCCEECHHHHHHHH
MAERQPATMARENIDTVNMPTCEAVAEHLNVPLATTVKTLIVQGHTPEGEPQLIAVVLRG
HHHCCCHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHEEECCCCCCCCEEEEEEEEC
DHTLNTIKAEKIEEANVPLMMATEEELKAAGLHKGYIGVELNMPVFVDRAAAALSDFVSG
CCCCCHHHHHHHHHCCCCEEEECHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
ANEINKHTTGMNWERDAHITRIVDIRNVHEGDPSPDGKGTLQIKRGIEVGHIFQLGDKYS
HHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHH
QAMNCTVSGEDGKPVTLMMGCYGIGVSRIIAAAIEQNNDENGIMWPSTPDISDSLAPFEV
HHEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEE
AIVPMKSKEDTVMETATALYEELKALGINVLLDDRNERPGVKFADLELIGIPHRIVVSDR
EEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEECCCEEEEECCC
NLAEDKYEYVNRRDSEKQLLSRDEVIAKVSGK
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA