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The map label for this gene is phrB [H]

Identifier: 93005295

GI number: 93005295

Start: 570378

End: 572003

Strand: Reverse

Name: phrB [H]

Synonym: Pcryo_0465

Alternate gene names: 93005295

Gene position: 572003-570378 (Counterclockwise)

Preceding gene: 93005303

Following gene: 93005293

Centisome position: 18.69

GC content: 42.44

Gene sequence:

>1626_bases
ATGTCTAAAACGGCGGTAGCGATAAAAAATAATTCACACAACAAAGTCGAGGATAGTCATCAGTTTCATTATCTCATGTG
GTTTCGCCGTGATCTACGCGTTCATGACAATACTGCTTTGGCTGCCATTTGTGAGCGTGCAAACGAAGACAATGCTCAGC
TCAGCGCGGTATTTTTTCTTACCCCTGAGCAGTGGCAGGCACATGACATGTCGCTTACACAGCTTGATCATATTGCGCGA
ACACTTCCTATCCTCGCAGAAAAACTGCATCAGTTGAACATCATTTTAACCGTGCAGGTCTGCCCTAGTTTTACTGATTG
TATAGTTACGTTGAGCGCATTATGTGAGTCAAATAATATCAGCTGCGTAATGGCAAATCATGAGTACGAAGGTAATGAGG
TTGCGCGGGATGAACTGTTAACCAAACAACTTGCAAACAACGAAATTGAGTTTATCCGCTGGCATGACCAATGTATCTTA
CCACCGAAAAGTATCACTACCAATGATGACAGCATGTATCAAGTGTTTACCCCATTTTATAAAAAATGGCGGCATACCTT
GGAGGTTAGTGACATACAAATTCATACAGCACTTGCCATCATAGATAATAAACAAGTTAATTTAACATTATCAGAACACA
GTGCAAACACTACTCAAGATATAGAAGCATTGTGCAAAAAAACTGTCCACGACTATCAAGAATATCTACAAACTCATGAA
TCCTATCAAAATATAGATACGGACAAGCAAATTAGCCAAGCGCGCCTTGCTTATCCTGCTGGCGAAGCGGAAGCCTGTCA
TCGATTAGAGCACTTTATCGCGGATGATATCGATAACTATGATGTGAGCCGTGATGTGCCGAGCTTACAGGCGACCAGTC
AATTATCAACCTATCTTACTATTGGCGCAATCAGTCCCAGACTTTGCTACTTACAAGCTACTCAGGCACTAGGGAAATTA
CGTAGAAATGATGGTGACAACTTTGACAGCGGTGACAATAACGACATCAACCGCTGGATAAGCGAGCTGGCTTGGCGAGA
TTTTTATCGCCATGTTTTAGACTATAAACCTGAGCTTATCCGCCATCAAGCTTATAAATATGAGACTGATAAGAAAATCA
ACTGGTCATATGATGAAGATGCTTTTGCAGCATGGCGTGAGGGCAAAACGGGTGTGCCATTAGTCGATGCAGCGATGCGC
TGCCTTAATGCGACAGGCTTTATGCACAATCGCTTGCGCATGGTTACCGCGATGTTTTTGACCAAAGATTTATTGATTGA
TTGGCGCTGGGGTGAGCGTTACTTTATGCAACAGCTGATAGACGGTGACTTTGCGTCTAATAATGGTGGCTGGCAGTGGA
GCGCATCAACAGGTACAGATTCTGCGCCCTATTTTCGTATTATGAATCCCTTTAGCCAAGCTAAAAGCCATGATACTGAG
GGAATATTCATTAAGACTTGGCTGCCTGAATTAAAACCGATTCCTACCAGCATTTTGCATAGAGAGGATAAAATGCGCAA
GGCTCTGAGTAAGAATGGTACCTTTGCAGATATTGATTATCCTGTGCCAATCGTTGAGCATAAACTGGCACGACAATTAG
TGATTGCTGAATTTAAAAAAGAGTAA

Upstream 100 bases:

>100_bases
TAATAAAGGAGCAAATCAATTGCATCCAAAAACTTATCCTAGCATACTTTTATTCATAGTTTTGTTTATGATTGCGATTG
ATTAATATGGGAATAGATGA

Downstream 100 bases:

>100_bases
TATCTATAGTTAAAATATCTCAAAAAACGCTACAGTGCTGCTGGTATAAATTTTCTGCAGCCTCTGTCTGCGTAGGCACA
GCAAGCAAGAAAAATGAATA

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSKTAVAIKNNSHNKVEDSHQFHYLMWFRRDLRVHDNTALAAICERANEDNAQLSAVFFLTPEQWQAHDMSLTQLDHIAR
TLPILAEKLHQLNIILTVQVCPSFTDCIVTLSALCESNNISCVMANHEYEGNEVARDELLTKQLANNEIEFIRWHDQCIL
PPKSITTNDDSMYQVFTPFYKKWRHTLEVSDIQIHTALAIIDNKQVNLTLSEHSANTTQDIEALCKKTVHDYQEYLQTHE
SYQNIDTDKQISQARLAYPAGEAEACHRLEHFIADDIDNYDVSRDVPSLQATSQLSTYLTIGAISPRLCYLQATQALGKL
RRNDGDNFDSGDNNDINRWISELAWRDFYRHVLDYKPELIRHQAYKYETDKKINWSYDEDAFAAWREGKTGVPLVDAAMR
CLNATGFMHNRLRMVTAMFLTKDLLIDWRWGERYFMQQLIDGDFASNNGGWQWSASTGTDSAPYFRIMNPFSQAKSHDTE
GIFIKTWLPELKPIPTSILHREDKMRKALSKNGTFADIDYPVPIVEHKLARQLVIAEFKKE

Sequences:

>Translated_541_residues
MSKTAVAIKNNSHNKVEDSHQFHYLMWFRRDLRVHDNTALAAICERANEDNAQLSAVFFLTPEQWQAHDMSLTQLDHIAR
TLPILAEKLHQLNIILTVQVCPSFTDCIVTLSALCESNNISCVMANHEYEGNEVARDELLTKQLANNEIEFIRWHDQCIL
PPKSITTNDDSMYQVFTPFYKKWRHTLEVSDIQIHTALAIIDNKQVNLTLSEHSANTTQDIEALCKKTVHDYQEYLQTHE
SYQNIDTDKQISQARLAYPAGEAEACHRLEHFIADDIDNYDVSRDVPSLQATSQLSTYLTIGAISPRLCYLQATQALGKL
RRNDGDNFDSGDNNDINRWISELAWRDFYRHVLDYKPELIRHQAYKYETDKKINWSYDEDAFAAWREGKTGVPLVDAAMR
CLNATGFMHNRLRMVTAMFLTKDLLIDWRWGERYFMQQLIDGDFASNNGGWQWSASTGTDSAPYFRIMNPFSQAKSHDTE
GIFIKTWLPELKPIPTSILHREDKMRKALSKNGTFADIDYPVPIVEHKLARQLVIAEFKKE
>Mature_540_residues
SKTAVAIKNNSHNKVEDSHQFHYLMWFRRDLRVHDNTALAAICERANEDNAQLSAVFFLTPEQWQAHDMSLTQLDHIART
LPILAEKLHQLNIILTVQVCPSFTDCIVTLSALCESNNISCVMANHEYEGNEVARDELLTKQLANNEIEFIRWHDQCILP
PKSITTNDDSMYQVFTPFYKKWRHTLEVSDIQIHTALAIIDNKQVNLTLSEHSANTTQDIEALCKKTVHDYQEYLQTHES
YQNIDTDKQISQARLAYPAGEAEACHRLEHFIADDIDNYDVSRDVPSLQATSQLSTYLTIGAISPRLCYLQATQALGKLR
RNDGDNFDSGDNNDINRWISELAWRDFYRHVLDYKPELIRHQAYKYETDKKINWSYDEDAFAAWREGKTGVPLVDAAMRC
LNATGFMHNRLRMVTAMFLTKDLLIDWRWGERYFMQQLIDGDFASNNGGWQWSASTGTDSAPYFRIMNPFSQAKSHDTEG
IFIKTWLPELKPIPTSILHREDKMRKALSKNGTFADIDYPVPIVEHKLARQLVIAEFKKE

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=522, Percent_Identity=24.5210727969349, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI4758072, Length=522, Percent_Identity=24.5210727969349, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI188536103, Length=287, Percent_Identity=30.3135888501742, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1786926, Length=522, Percent_Identity=38.8888888888889, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324962, Length=537, Percent_Identity=33.7057728119181, Blast_Score=267, Evalue=3e-72,
Organism=Drosophila melanogaster, GI17137248, Length=528, Percent_Identity=26.3257575757576, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24585455, Length=528, Percent_Identity=26.3257575757576, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24648152, Length=526, Percent_Identity=22.0532319391635, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 62474; Mature: 62343

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTAVAIKNNSHNKVEDSHQFHYLMWFRRDLRVHDNTALAAICERANEDNAQLSAVFFL
CCCCEEEEECCCCCCCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEE
TPEQWQAHDMSLTQLDHIARTLPILAEKLHQLNIILTVQVCPSFTDCIVTLSALCESNNI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCC
SCVMANHEYEGNEVARDELLTKQLANNEIEFIRWHDQCILPPKSITTNDDSMYQVFTPFY
EEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCHHHHHHHHH
KKWRHTLEVSDIQIHTALAIIDNKQVNLTLSEHSANTTQDIEALCKKTVHDYQEYLQTHE
HHHHHHEEECEEEEEEEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SYQNIDTDKQISQARLAYPAGEAEACHRLEHFIADDIDNYDVSRDVPSLQATSQLSTYLT
HHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHEEE
IGAISPRLCYLQATQALGKLRRNDGDNFDSGDNNDINRWISELAWRDFYRHVLDYKPELI
EECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
RHQAYKYETDKKINWSYDEDAFAAWREGKTGVPLVDAAMRCLNATGFMHNRLRMVTAMFL
HHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKDLLIDWRWGERYFMQQLIDGDFASNNGGWQWSASTGTDSAPYFRIMNPFSQAKSHDTE
HHHHEEEEECCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECCCHHHHCCCCCC
GIFIKTWLPELKPIPTSILHREDKMRKALSKNGTFADIDYPVPIVEHKLARQLVIAEFKK
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCC
E
C
>Mature Secondary Structure 
SKTAVAIKNNSHNKVEDSHQFHYLMWFRRDLRVHDNTALAAICERANEDNAQLSAVFFL
CCCEEEEECCCCCCCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEE
TPEQWQAHDMSLTQLDHIARTLPILAEKLHQLNIILTVQVCPSFTDCIVTLSALCESNNI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCC
SCVMANHEYEGNEVARDELLTKQLANNEIEFIRWHDQCILPPKSITTNDDSMYQVFTPFY
EEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCHHHHHHHHH
KKWRHTLEVSDIQIHTALAIIDNKQVNLTLSEHSANTTQDIEALCKKTVHDYQEYLQTHE
HHHHHHEEECEEEEEEEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SYQNIDTDKQISQARLAYPAGEAEACHRLEHFIADDIDNYDVSRDVPSLQATSQLSTYLT
HHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHEEE
IGAISPRLCYLQATQALGKLRRNDGDNFDSGDNNDINRWISELAWRDFYRHVLDYKPELI
EECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
RHQAYKYETDKKINWSYDEDAFAAWREGKTGVPLVDAAMRCLNATGFMHNRLRMVTAMFL
HHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKDLLIDWRWGERYFMQQLIDGDFASNNGGWQWSASTGTDSAPYFRIMNPFSQAKSHDTE
HHHHEEEEECCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECCCHHHHCCCCCC
GIFIKTWLPELKPIPTSILHREDKMRKALSKNGTFADIDYPVPIVEHKLARQLVIAEFKK
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]