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The map label for this gene is cobB2 [H]

Identifier: 93005287

GI number: 93005287

Start: 561244

End: 562044

Strand: Reverse

Name: cobB2 [H]

Synonym: Pcryo_0457

Alternate gene names: 93005287

Gene position: 562044-561244 (Counterclockwise)

Preceding gene: 93005293

Following gene: 93005282

Centisome position: 18.37

GC content: 45.82

Gene sequence:

>801_bases
ATGTTAGCAGAATTATCATCAGCGTTAATAGAAGAAGTAGAGCGTTCTGCTGAACTATTAGAATCGAAACAGCATATTTG
CCTTTTAACTGGCGCAGGGATTTCAGCAGAAAGCGGCATTCCGACTTTTCGTGATAAGCAAACTGGCTTGTGGGAAAATT
ATCGGGCTGAGGACTTGGCGACACCAGATGCCTTCGCCCGTGATCCAAAGCTGGTTTGGTCATGGTATCAATGGCGCAGA
CAGCTCGTGGCTAGTAAAAAACCCAATCCTGCCCATACAGCATTAGCGCAATGGCAGTATCACAGTCAATCGACTAATCA
GCAACTGACCCTCATTACTCAAAATGTCGATGATTTGCATGAGCAAGCAGGCAGCGCTGTTACCCACCTGCATGGCAATC
TATGGCGTAATCGATGCAGTCAATGTCAGACACCTTACACAATCCAATTAAAAAGCGCTTATGACAGCGAGAATAGCATC
AGCTTTGATGAAGCTCTAATGACTTGCCAGCATTGTGATGGTTATATCAGACCTGATATTGTGTGGTTTGGAGAGTCGTT
ACCGATGCGGGCATGGCAGACAGCAGAGGAAGCTGCCGCGAATTGTGATGTATTTATTAGTATCGGTACTTCCAGCCTCG
TCTATCCCGCTGCTGGATTGGCACAACTGGCAAAACATAACGGTGCCAAAATTATCGAGATAAATCCTTATCCGACACCA
AATACGCTAGTCGATATTACTCTGGCAGCAAAAGCTGGTGCCATCCTGCCAGAGTTGGTAAAAAGAATGGGCATACTATG
A

Upstream 100 bases:

>100_bases
GCAAAAAACCATAATCCAACGATAACTGCCATCAATATGACAAGCCACCAAATTCCGTCTATCATAAGAAAGGATATCCA
TTATTAGCAAGGGTTAGCTT

Downstream 100 bases:

>100_bases
TATAACTGGCATCCCATTAGCCTACCGCTTGATCGACTCTATTTAAATACTGCTGCTTTAGCTCATCACTGATAAATGAC
GATCTAAAAGAGTTCTTTAC

Product: silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 2 [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLAELSSALIEEVERSAELLESKQHICLLTGAGISAESGIPTFRDKQTGLWENYRAEDLATPDAFARDPKLVWSWYQWRR
QLVASKKPNPAHTALAQWQYHSQSTNQQLTLITQNVDDLHEQAGSAVTHLHGNLWRNRCSQCQTPYTIQLKSAYDSENSI
SFDEALMTCQHCDGYIRPDIVWFGESLPMRAWQTAEEAAANCDVFISIGTSSLVYPAAGLAQLAKHNGAKIIEINPYPTP
NTLVDITLAAKAGAILPELVKRMGIL

Sequences:

>Translated_266_residues
MLAELSSALIEEVERSAELLESKQHICLLTGAGISAESGIPTFRDKQTGLWENYRAEDLATPDAFARDPKLVWSWYQWRR
QLVASKKPNPAHTALAQWQYHSQSTNQQLTLITQNVDDLHEQAGSAVTHLHGNLWRNRCSQCQTPYTIQLKSAYDSENSI
SFDEALMTCQHCDGYIRPDIVWFGESLPMRAWQTAEEAAANCDVFISIGTSSLVYPAAGLAQLAKHNGAKIIEINPYPTP
NTLVDITLAAKAGAILPELVKRMGIL
>Mature_266_residues
MLAELSSALIEEVERSAELLESKQHICLLTGAGISAESGIPTFRDKQTGLWENYRAEDLATPDAFARDPKLVWSWYQWRR
QLVASKKPNPAHTALAQWQYHSQSTNQQLTLITQNVDDLHEQAGSAVTHLHGNLWRNRCSQCQTPYTIQLKSAYDSENSI
SFDEALMTCQHCDGYIRPDIVWFGESLPMRAWQTAEEAAANCDVFISIGTSSLVYPAAGLAQLAKHNGAKIIEINPYPTP
NTLVDITLAAKAGAILPELVKRMGIL

Specific function: Modulates the activity of chromatin proteins, thereby having an effect on transcription per subunit [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=243, Percent_Identity=36.2139917695473, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI6912664, Length=257, Percent_Identity=36.5758754863813, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI13787215, Length=240, Percent_Identity=37.5, Blast_Score=171, Evalue=4e-43,
Organism=Homo sapiens, GI6912662, Length=275, Percent_Identity=28.7272727272727, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI6912660, Length=228, Percent_Identity=30.2631578947368, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI300797577, Length=266, Percent_Identity=27.8195488721804, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI7657575, Length=270, Percent_Identity=27.037037037037, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI63054862, Length=214, Percent_Identity=30.3738317757009, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI13775602, Length=265, Percent_Identity=28.3018867924528, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI13775600, Length=265, Percent_Identity=28.3018867924528, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI300797705, Length=224, Percent_Identity=29.0178571428571, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI7706712, Length=217, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI308199517, Length=246, Percent_Identity=40.2439024390244, Blast_Score=162, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17541892, Length=262, Percent_Identity=29.3893129770992, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17567771, Length=278, Percent_Identity=27.3381294964029, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71990482, Length=278, Percent_Identity=26.2589928057554, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71990487, Length=280, Percent_Identity=26.0714285714286, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6324504, Length=249, Percent_Identity=25.3012048192771, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325242, Length=220, Percent_Identity=28.6363636363636, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320163, Length=290, Percent_Identity=23.7931034482759, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648389, Length=211, Percent_Identity=32.7014218009479, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571445, Length=279, Percent_Identity=27.9569892473118, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137536, Length=279, Percent_Identity=25.8064516129032, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 29551; Mature: 29551

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAELSSALIEEVERSAELLESKQHICLLTGAGISAESGIPTFRDKQTGLWENYRAEDLA
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCC
TPDAFARDPKLVWSWYQWRRQLVASKKPNPAHTALAQWQYHSQSTNQQLTLITQNVDDLH
CCHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHH
EQAGSAVTHLHGNLWRNRCSQCQTPYTIQLKSAYDSENSISFDEALMTCQHCDGYIRPDI
HHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCE
VWFGESLPMRAWQTAEEAAANCDVFISIGTSSLVYPAAGLAQLAKHNGAKIIEINPYPTP
EEECCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHCHHHHHHHHHCCCCEEEEECCCCCC
NTLVDITLAAKAGAILPELVKRMGIL
CCEEEEEECCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLAELSSALIEEVERSAELLESKQHICLLTGAGISAESGIPTFRDKQTGLWENYRAEDLA
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCC
TPDAFARDPKLVWSWYQWRRQLVASKKPNPAHTALAQWQYHSQSTNQQLTLITQNVDDLH
CCHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHH
EQAGSAVTHLHGNLWRNRCSQCQTPYTIQLKSAYDSENSISFDEALMTCQHCDGYIRPDI
HHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCE
VWFGESLPMRAWQTAEEAAANCDVFISIGTSSLVYPAAGLAQLAKHNGAKIIEINPYPTP
EEECCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHCHHHHHHHHHCCCCEEEEECCCCCC
NTLVDITLAAKAGAILPELVKRMGIL
CCEEEEEECCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]