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The map label for this gene is artJ [H]

Identifier: 93005158

GI number: 93005158

Start: 395224

End: 396141

Strand: Direct

Name: artJ [H]

Synonym: Pcryo_0327

Alternate gene names: 93005158

Gene position: 395224-396141 (Clockwise)

Preceding gene: 93005157

Following gene: 93005159

Centisome position: 12.92

GC content: 44.66

Gene sequence:

>918_bases
ATGAGTAACTATCTTGTCATAGGCTCAAATTCAGTGACCAGTCAGCGGTTTTCAAAACCTATGCTAGCAGTGGCAGCATT
TTTAGCTTTAGTCGGTTGTAGTAACGGTCAAGATAAAGGGGCTGAAAGGACTAACTCAGACGCTAATAATACTGCGGAGA
CAGCTGAGAAAAAAGCAGATGCGACAGAGGGTAATGTGCTGCGTATCGGTACTGAGGGTGCTTATGCGCCCTTTAACTAT
ACCAATGCAGATGGCACGCTCGGCGGTTTTGATATCGAAATTGCCAATGCTATCTGTGCTGATATGCAAGTAACTTGTGA
GATTGTGGCGCAAGATTGGGACGGTATCATCCCCGGTCTCAAGGCTGGTAAATATGACGCTATCGTCGCTGCGATGTCAG
TCACCCCTGAGCGCGCTCAACAAGTTGCTTTTACGGATCCGTATTTTAGCAATGCGCTAGTGTTTTTAGCCAAAAAAGAT
AGCACCTTTGACCCTGCTAAGGTGACTGATATTAATGCCCACTCTATCGCCGCTCAGCGCTCCACCATCTCATCACAGTG
GCTAGAAAACACTTATCCAAAAGCGGATATGAAGCTATATGATACATTAAGTAATGCGTTTTTAGATTTGGGTGCTGGTC
GCGTCGATGCCATGATATCGGATAAACTGCCAGCGATTGAATGGCTCAGCTCGACGTCCGGTAATAATTATGTGTTAAAG
GGCGCGGAGATTGATATCAATGATAACTTTGCGATTGCCGTCCGTCCGAATGATGAAGCTCTACAAGCAAAAATTAATAC
CTCATTGAGCAATATTAAAGCCAACGGCACTTATGATAAGATCAATCAAAAATACTTTGCTGTGCCAGCAAGTGCGACAC
TAACAGAAGCACCAGCTATAGATAGTGCTACACAGTAA

Upstream 100 bases:

>100_bases
ATACCTGTGATGACAAATATGTATTGATCCTAAGTTTTGAGGAATGCATATTTGCCAGCACTTATCAAAATTTCACGACG
TAATAAACTCAAGGATGATT

Downstream 100 bases:

>100_bases
AGGCTCAGTAGTATTACGGTTTTCACGACTGCATTAATATTGTGTTAATAAGCATGAGGACATCACCATTATGGTGATGT
CCTTTTTCTTGCGTACTTAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSNYLVIGSNSVTSQRFSKPMLAVAAFLALVGCSNGQDKGAERTNSDANNTAETAEKKADATEGNVLRIGTEGAYAPFNY
TNADGTLGGFDIEIANAICADMQVTCEIVAQDWDGIIPGLKAGKYDAIVAAMSVTPERAQQVAFTDPYFSNALVFLAKKD
STFDPAKVTDINAHSIAAQRSTISSQWLENTYPKADMKLYDTLSNAFLDLGAGRVDAMISDKLPAIEWLSSTSGNNYVLK
GAEIDINDNFAIAVRPNDEALQAKINTSLSNIKANGTYDKINQKYFAVPASATLTEAPAIDSATQ

Sequences:

>Translated_305_residues
MSNYLVIGSNSVTSQRFSKPMLAVAAFLALVGCSNGQDKGAERTNSDANNTAETAEKKADATEGNVLRIGTEGAYAPFNY
TNADGTLGGFDIEIANAICADMQVTCEIVAQDWDGIIPGLKAGKYDAIVAAMSVTPERAQQVAFTDPYFSNALVFLAKKD
STFDPAKVTDINAHSIAAQRSTISSQWLENTYPKADMKLYDTLSNAFLDLGAGRVDAMISDKLPAIEWLSSTSGNNYVLK
GAEIDINDNFAIAVRPNDEALQAKINTSLSNIKANGTYDKINQKYFAVPASATLTEAPAIDSATQ
>Mature_304_residues
SNYLVIGSNSVTSQRFSKPMLAVAAFLALVGCSNGQDKGAERTNSDANNTAETAEKKADATEGNVLRIGTEGAYAPFNYT
NADGTLGGFDIEIANAICADMQVTCEIVAQDWDGIIPGLKAGKYDAIVAAMSVTPERAQQVAFTDPYFSNALVFLAKKDS
TFDPAKVTDINAHSIAAQRSTISSQWLENTYPKADMKLYDTLSNAFLDLGAGRVDAMISDKLPAIEWLSSTSGNNYVLKG
AEIDINDNFAIAVRPNDEALQAKINTSLSNIKANGTYDKINQKYFAVPASATLTEAPAIDSATQ

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=234, Percent_Identity=36.3247863247863, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1787088, Length=230, Percent_Identity=33.4782608695652, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1788649, Length=226, Percent_Identity=35.3982300884956, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788648, Length=227, Percent_Identity=32.5991189427313, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1788228, Length=225, Percent_Identity=25.3333333333333, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787031, Length=221, Percent_Identity=27.6018099547511, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 32510; Mature: 32379

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYLVIGSNSVTSQRFSKPMLAVAAFLALVGCSNGQDKGAERTNSDANNTAETAEKKAD
CCCEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
ATEGNVLRIGTEGAYAPFNYTNADGTLGGFDIEIANAICADMQVTCEIVAQDWDGIIPGL
CCCCCEEEEECCCCCCCCEECCCCCCCCCEEEEEHHHHHCCHHEEEEEEECCCCCCCCCC
KAGKYDAIVAAMSVTPERAQQVAFTDPYFSNALVFLAKKDSTFDPAKVTDINAHSIAAQR
CCCCHHEEEEEEECCHHHHHEEEECCCCCCCEEEEEEECCCCCCCCEEEECCHHHHHHHH
STISSQWLENTYPKADMKLYDTLSNAFLDLGAGRVDAMISDKLPAIEWLSSTSGNNYVLK
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHCCCCCEEEEE
GAEIDINDNFAIAVRPNDEALQAKINTSLSNIKANGTYDKINQKYFAVPASATLTEAPAI
CEEEEECCCEEEEECCCCHHHHEECCCCHHHEECCCCHHHCCCEEEEECCCCCCCCCCCC
DSATQ
CCCCC
>Mature Secondary Structure 
SNYLVIGSNSVTSQRFSKPMLAVAAFLALVGCSNGQDKGAERTNSDANNTAETAEKKAD
CCEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
ATEGNVLRIGTEGAYAPFNYTNADGTLGGFDIEIANAICADMQVTCEIVAQDWDGIIPGL
CCCCCEEEEECCCCCCCCEECCCCCCCCCEEEEEHHHHHCCHHEEEEEEECCCCCCCCCC
KAGKYDAIVAAMSVTPERAQQVAFTDPYFSNALVFLAKKDSTFDPAKVTDINAHSIAAQR
CCCCHHEEEEEEECCHHHHHEEEECCCCCCCEEEEEEECCCCCCCCEEEECCHHHHHHHH
STISSQWLENTYPKADMKLYDTLSNAFLDLGAGRVDAMISDKLPAIEWLSSTSGNNYVLK
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHCCCCCEEEEE
GAEIDINDNFAIAVRPNDEALQAKINTSLSNIKANGTYDKINQKYFAVPASATLTEAPAI
CEEEEECCCEEEEECCCCHHHHEECCCCHHHEECCCCHHHCCCEEEEECCCCCCCCCCCC
DSATQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]