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The map label for this gene is artI [H]

Identifier: 93005157

GI number: 93005157

Start: 394192

End: 395124

Strand: Direct

Name: artI [H]

Synonym: Pcryo_0326

Alternate gene names: 93005157

Gene position: 394192-395124 (Clockwise)

Preceding gene: 93005156

Following gene: 93005158

Centisome position: 12.88

GC content: 43.62

Gene sequence:

>933_bases
ATGATCATTTCAATGACCTCGTCAATGACCAAAAAAGCCTTGTGGTTAGCGCCACTAAGCGCTGCTATGCTGATGCTAGC
TGGCTGTAATAACAGCTCTACACCAACAGAAAGCACTGAAACAGCTGCAGCAACTGATACGCCTTTAAATATCAAAATCG
CGACGGAATCAAGCTATAAACCTTTTAGTTATACCGATGCTGATGGCAAGTTAATCGGCTATGAGATTGAATTGGTTGAC
GCACTATGTGCGCAAATGAAAGCCAAATGCGAAGTGATCTCCCAGGATTGGGATGGCTTGATTCCAGGCTTAAATGCGCA
GAAGTTTGATGCTGCCATCGCTGGTATGTCAATCACCCCTGAGCGCAAAGAAGTGGTCGAATTTAGCGAACCTTACTTTC
ATAGCGGCATTATCTTAATCGGTAAAAAAGGCGACGATGTTAGTGTTGACGCTCTAAAAGGTCAGCCAATTGCCTCACAG
CGTTCAACTGTTGCCTCGCAATATCTACAAGATAAACATGCTGATGCTGATATCAAACTTTATGATACCCAAGACAATGC
GTATCTGGATCTAACGTCAGGTCGTGTGCGGGCGATGATGTCTGATAAAGTGACTGGTATTGACTGGCTAAAAACAGAAG
CTGGTAAAGACTATGAGGTCAAAGGTCAAGAAATCAGCACCAATGATGATGCGATGGGTATCGCTTTCCGTAAAGGCGAT
CCATTGGTTGCCAAATTTAATAAAGCCTTGGCTGAGCTAAAAGACAATGGCACTTATGATCAAATCACGGGTAGCTATTT
TGGTACTAGCTCAACCACTGCTGCTCAAAAAGCAGTCGCTACTAGTGATATCAAAGAAGTAGTAGTCATGGATAAAGCTA
GTGTCGATGCTGATACGGCGATTGCTAAAGAAGAGCAAGCGACAGCAAACTAA

Upstream 100 bases:

>100_bases
ACTTTGGTCAATAATCTGTAATCATTAGCGTCGATTGTTTATATCCGTTTTAAGCAGCGGATATATATACGGCTGGTAAA
TATATTAAGGAATAACCACT

Downstream 100 bases:

>100_bases
TACCTGTGATGACAAATATGTATTGATCCTAAGTTTTGAGGAATGCATATTTGCCAGCACTTATCAAAATTTCACGACGT
AATAAACTCAAGGATGATTA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MIISMTSSMTKKALWLAPLSAAMLMLAGCNNSSTPTESTETAAATDTPLNIKIATESSYKPFSYTDADGKLIGYEIELVD
ALCAQMKAKCEVISQDWDGLIPGLNAQKFDAAIAGMSITPERKEVVEFSEPYFHSGIILIGKKGDDVSVDALKGQPIASQ
RSTVASQYLQDKHADADIKLYDTQDNAYLDLTSGRVRAMMSDKVTGIDWLKTEAGKDYEVKGQEISTNDDAMGIAFRKGD
PLVAKFNKALAELKDNGTYDQITGSYFGTSSTTAAQKAVATSDIKEVVVMDKASVDADTAIAKEEQATAN

Sequences:

>Translated_310_residues
MIISMTSSMTKKALWLAPLSAAMLMLAGCNNSSTPTESTETAAATDTPLNIKIATESSYKPFSYTDADGKLIGYEIELVD
ALCAQMKAKCEVISQDWDGLIPGLNAQKFDAAIAGMSITPERKEVVEFSEPYFHSGIILIGKKGDDVSVDALKGQPIASQ
RSTVASQYLQDKHADADIKLYDTQDNAYLDLTSGRVRAMMSDKVTGIDWLKTEAGKDYEVKGQEISTNDDAMGIAFRKGD
PLVAKFNKALAELKDNGTYDQITGSYFGTSSTTAAQKAVATSDIKEVVVMDKASVDADTAIAKEEQATAN
>Mature_310_residues
MIISMTSSMTKKALWLAPLSAAMLMLAGCNNSSTPTESTETAAATDTPLNIKIATESSYKPFSYTDADGKLIGYEIELVD
ALCAQMKAKCEVISQDWDGLIPGLNAQKFDAAIAGMSITPERKEVVEFSEPYFHSGIILIGKKGDDVSVDALKGQPIASQ
RSTVASQYLQDKHADADIKLYDTQDNAYLDLTSGRVRAMMSDKVTGIDWLKTEAGKDYEVKGQEISTNDDAMGIAFRKGD
PLVAKFNKALAELKDNGTYDQITGSYFGTSSTTAAQKAVATSDIKEVVVMDKASVDADTAIAKEEQATAN

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=247, Percent_Identity=34.412955465587, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788649, Length=239, Percent_Identity=35.1464435146443, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787085, Length=220, Percent_Identity=35.9090909090909, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1787031, Length=220, Percent_Identity=33.1818181818182, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788228, Length=224, Percent_Identity=30.8035714285714, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 33293; Mature: 33293

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISMTSSMTKKALWLAPLSAAMLMLAGCNNSSTPTESTETAAATDTPLNIKIATESSYK
CEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
PFSYTDADGKLIGYEIELVDALCAQMKAKCEVISQDWDGLIPGLNAQKFDAAIAGMSITP
CCEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCC
ERKEVVEFSEPYFHSGIILIGKKGDDVSVDALKGQPIASQRSTVASQYLQDKHADADIKL
CHHHHHHCCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
YDTQDNAYLDLTSGRVRAMMSDKVTGIDWLKTEAGKDYEVKGQEISTNDDAMGIAFRKGD
EECCCCEEEEECCCCEEEEHHCCCCCCHHHHHCCCCCEEECCCEECCCCCCEEEEEECCC
PLVAKFNKALAELKDNGTYDQITGSYFGTSSTTAAQKAVATSDIKEVVVMDKASVDADTA
CHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCHHH
IAKEEQATAN
HHCCHHCCCC
>Mature Secondary Structure
MIISMTSSMTKKALWLAPLSAAMLMLAGCNNSSTPTESTETAAATDTPLNIKIATESSYK
CEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
PFSYTDADGKLIGYEIELVDALCAQMKAKCEVISQDWDGLIPGLNAQKFDAAIAGMSITP
CCEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCC
ERKEVVEFSEPYFHSGIILIGKKGDDVSVDALKGQPIASQRSTVASQYLQDKHADADIKL
CHHHHHHCCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
YDTQDNAYLDLTSGRVRAMMSDKVTGIDWLKTEAGKDYEVKGQEISTNDDAMGIAFRKGD
EECCCCEEEEECCCCEEEEHHCCCCCCHHHHHCCCCCEEECCCEECCCCCCEEEEEECCC
PLVAKFNKALAELKDNGTYDQITGSYFGTSSTTAAQKAVATSDIKEVVVMDKASVDADTA
CHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCHHH
IAKEEQATAN
HHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]