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The map label for this gene is artJ [H]

Identifier: 93005156

GI number: 93005156

Start: 393305

End: 394105

Strand: Direct

Name: artJ [H]

Synonym: Pcryo_0325

Alternate gene names: 93005156

Gene position: 393305-394105 (Clockwise)

Preceding gene: 93005155

Following gene: 93005157

Centisome position: 12.85

GC content: 44.69

Gene sequence:

>801_bases
ATGATGTCGAATTCTCGCCTATTATGGTCATCGATTACCGTTACTGCCGCGCTCATGTTAGGTGCGTGTAGCCAACCTGC
CAATGACGCTGCTGATACCAATGCAGATGCACCGGCAGCAGGAACCACTGGCAAGACCATTCGTATTGCGACTGAAGGCG
CATACCCTCCTTTTAACTATACCAATGCCGATGGCAGTTTGGCGGGTTTCGATATCGACGTTGCCAATGCTCTATGTGAG
CAGATGCAAGCCAAATGTGAAATTGTCGCTCAAGATTGGGACGGTATTATTCCGGGTTTATTGGCGCAAAAATACGATGC
GGTTATCGCTGGTATGTCTATTACCGCTGAACGTCAAGAAAAAGTCGATTTTAGTGAGCCTTACTTTGCCAATACGATGG
TTTGGCTGACCGATACCAAAGGCAGCTTTGATCCTAAAGTCATCAAAAATCTGACGCTTGGTGGTCAACGTTCAACGACA
CCAGGTGCTTATTTACAAGACAATTATGAAGGCAAAGATGGCAATACCGTTAAGCTTTATGACAATTATGACAATGCTTA
TTTGGACCTGAAGTCTGGTCGTAGTGATGTTGTATTGGCTGAAAAAGTATCAGCAAAATCATGGTTAGCAGACAACCCTG
AAGGCTTCGGTATCGTGGGTGATGAGATTGACAATGATGACAATATTGCGATTGCTGTCCGTAAAGGCGATGCCATTAAA
GAAGACTTTAATAAAGCGCTGAATGAAATTCGCAGTAACGGTGAGCTTGCCCGTCTTGAGCAGCAAAACTTTGGTCAATA
A

Upstream 100 bases:

>100_bases
ACTTATCTGGCAGGGACGTCAGAGAAAATATACCAGAACGCTGTATTAAAAATTAGAGATTAAAAATCATCTTAAAAATC
CATCAGAATTTAAGGAACGT

Downstream 100 bases:

>100_bases
TCTGTAATCATTAGCGTCGATTGTTTATATCCGTTTTAAGCAGCGGATATATATACGGCTGGTAAATATATTAAGGAATA
ACCACTATGATCATTTCAAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MMSNSRLLWSSITVTAALMLGACSQPANDAADTNADAPAAGTTGKTIRIATEGAYPPFNYTNADGSLAGFDIDVANALCE
QMQAKCEIVAQDWDGIIPGLLAQKYDAVIAGMSITAERQEKVDFSEPYFANTMVWLTDTKGSFDPKVIKNLTLGGQRSTT
PGAYLQDNYEGKDGNTVKLYDNYDNAYLDLKSGRSDVVLAEKVSAKSWLADNPEGFGIVGDEIDNDDNIAIAVRKGDAIK
EDFNKALNEIRSNGELARLEQQNFGQ

Sequences:

>Translated_266_residues
MMSNSRLLWSSITVTAALMLGACSQPANDAADTNADAPAAGTTGKTIRIATEGAYPPFNYTNADGSLAGFDIDVANALCE
QMQAKCEIVAQDWDGIIPGLLAQKYDAVIAGMSITAERQEKVDFSEPYFANTMVWLTDTKGSFDPKVIKNLTLGGQRSTT
PGAYLQDNYEGKDGNTVKLYDNYDNAYLDLKSGRSDVVLAEKVSAKSWLADNPEGFGIVGDEIDNDDNIAIAVRKGDAIK
EDFNKALNEIRSNGELARLEQQNFGQ
>Mature_266_residues
MMSNSRLLWSSITVTAALMLGACSQPANDAADTNADAPAAGTTGKTIRIATEGAYPPFNYTNADGSLAGFDIDVANALCE
QMQAKCEIVAQDWDGIIPGLLAQKYDAVIAGMSITAERQEKVDFSEPYFANTMVWLTDTKGSFDPKVIKNLTLGGQRSTT
PGAYLQDNYEGKDGNTVKLYDNYDNAYLDLKSGRSDVVLAEKVSAKSWLADNPEGFGIVGDEIDNDDNIAIAVRKGDAIK
EDFNKALNEIRSNGELARLEQQNFGQ

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=233, Percent_Identity=36.480686695279, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1787088, Length=220, Percent_Identity=35.9090909090909, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788649, Length=229, Percent_Identity=34.4978165938865, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=31.8777292576419, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1788228, Length=227, Percent_Identity=29.0748898678414, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787031, Length=241, Percent_Identity=29.8755186721992, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28701; Mature: 28701

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSNSRLLWSSITVTAALMLGACSQPANDAADTNADAPAAGTTGKTIRIATEGAYPPFNY
CCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
TNADGSLAGFDIDVANALCEQMQAKCEIVAQDWDGIIPGLLAQKYDAVIAGMSITAERQE
CCCCCCEEECCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCEEECCHHH
KVDFSEPYFANTMVWLTDTKGSFDPKVIKNLTLGGQRSTTPGAYLQDNYEGKDGNTVKLY
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCEEEEE
DNYDNAYLDLKSGRSDVVLAEKVSAKSWLADNPEGFGIVGDEIDNDDNIAIAVRKGDAIK
ECCCCEEEEECCCCCCEEEEEHHCHHHHCCCCCCCCEEECCCCCCCCCEEEEEECCCHHH
EDFNKALNEIRSNGELARLEQQNFGQ
HHHHHHHHHHHCCCCEEEECHHCCCC
>Mature Secondary Structure
MMSNSRLLWSSITVTAALMLGACSQPANDAADTNADAPAAGTTGKTIRIATEGAYPPFNY
CCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
TNADGSLAGFDIDVANALCEQMQAKCEIVAQDWDGIIPGLLAQKYDAVIAGMSITAERQE
CCCCCCEEECCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCEEECCHHH
KVDFSEPYFANTMVWLTDTKGSFDPKVIKNLTLGGQRSTTPGAYLQDNYEGKDGNTVKLY
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCEEEEE
DNYDNAYLDLKSGRSDVVLAEKVSAKSWLADNPEGFGIVGDEIDNDDNIAIAVRKGDAIK
ECCCCEEEEECCCCCCEEEEEHHCHHHHCCCCCCCCEEECCCCCCCCCEEEEEECCCHHH
EDFNKALNEIRSNGELARLEQQNFGQ
HHHHHHHHHHHCCCCEEEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]