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The map label for this gene is pilT [H]

Identifier: 93005124

GI number: 93005124

Start: 351251

End: 352303

Strand: Reverse

Name: pilT [H]

Synonym: Pcryo_0293

Alternate gene names: 93005124

Gene position: 352303-351251 (Counterclockwise)

Preceding gene: 93005126

Following gene: 93005123

Centisome position: 11.51

GC content: 44.16

Gene sequence:

>1053_bases
ATGGCTGAACAAAATTTAAGCATTGAAGACTTGCTGCGTTTTACTGTCAAGCATAAGGCTTCAGATTTACATATTTCAGC
CGGTATGCCTGCGATGATTCGAGTTGATGGTGAGATGACTCGGATTAATATGCCAGATATGAGCCATAAGGTAGTTCATC
AGCTCATTTATGACATCATGAATGATAGACAACGTGCCGACTTTGAAGAGTTTTTTGAAACGGATTTTTCTTTTGAGATT
CCAAATCTAGCCCGTTTTCGGGTAAACGCATTTAATCAAAATCGCGGTGCAGGCGCCGTCTTTCGTACAATTCCTTCCAA
AGTTCTAACGATGGAAGATCTAGGTATGGGCGAAGTGTTTAAGCGTGTTTCAGACTTTAAACGTGGCGTGGTGTTGGTCA
CTGGTCCAACCGGCTCAGGTAAGTCGACCACGCTAGCTGCCATGATTGATTATATCAATGAAACCCGTAAAGAGCATATT
TTGACTATCGAAGACCCGATTGAATTTGTTCATGAGTCCAAAAAGAGTTTGATTAACCAGCGTGAAGTCCATCGCGATAC
CTTGGGGTTCGAGCCAGCATTGCGCTCAGCGTTACGTGAAGATCCGGACGTTATTCTGGTTGGTGAGCTACGAGATCTTG
AGACCATACGCTTAGCGCTCACTGCCGCTGAAACCGGTCACTTAGTTTTCGGCACTTTGCACACCAGTTCCGCTGCCAAA
ACCATTGACCGTGTTATTGACGTCTTCCCTGCTGCTGAAAAAGACATGATTCGAGCGATGCTATCAGAGTCACTACAAGC
CGTTATCTCGCAAACATTGTTACGTCGTCAAACAGGCGGGCGAATTGCCGCCCATGAGATTATGTTAGGTACGCCCGCTA
TTCGTAACTTAATACGAGAAAATAAAATCGCTCAAATGTACTCTTCTATCCAAACTGGTGCTGGTGATGGCATGATTACT
CTTGATCAAACTCTTAAAAATCTTGTAGCGAGAGGCACAATTAGTAAAGACGTTGCACGACCTTACGCTAAACAGCCTGA
AGCATTCTTATAA

Upstream 100 bases:

>100_bases
TTTAATGAGGTTGGCTCAAACAATGTCATAGAACCTTGTTTATCGCCTGCTTTCTATGTTGATTTTGGCACACTTTATTT
ATTAATAGGAATATCCGATT

Downstream 100 bases:

>100_bases
TATAAATATTATCAGGCATTCTGACTAAAGTAATAGACTAATCATTGCAGCTATCTAAGCAATCATCCTTTAAGTTAACT
GTCATTTTTCGCAAAAAAGT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MAEQNLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPDMSHKVVHQLIYDIMNDRQRADFEEFFETDFSFEI
PNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEHI
LTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAK
TIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRIAAHEIMLGTPAIRNLIRENKIAQMYSSIQTGAGDGMIT
LDQTLKNLVARGTISKDVARPYAKQPEAFL

Sequences:

>Translated_350_residues
MAEQNLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPDMSHKVVHQLIYDIMNDRQRADFEEFFETDFSFEI
PNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEHI
LTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAK
TIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRIAAHEIMLGTPAIRNLIRENKIAQMYSSIQTGAGDGMIT
LDQTLKNLVARGTISKDVARPYAKQPEAFL
>Mature_349_residues
AEQNLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPDMSHKVVHQLIYDIMNDRQRADFEEFFETDFSFEIP
NLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEHIL
TIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKT
IDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRIAAHEIMLGTPAIRNLIRENKIAQMYSSIQTGAGDGMITL
DQTLKNLVARGTISKDVARPYAKQPEAFL

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=331, Percent_Identity=45.9214501510574, Blast_Score=293, Evalue=9e-81,
Organism=Escherichia coli, GI1789723, Length=356, Percent_Identity=33.1460674157303, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1786296, Length=342, Percent_Identity=32.1637426900585, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39107; Mature: 38975

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQNLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPDMSHKVVHQLIYDIM
CCCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHH
NDRQRADFEEFFETDFSFEIPNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVF
CCCCCCCHHHHHCCCCCCCCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
KRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEHILTIEDPIEFVHESKKSLINQ
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHH
REVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAK
HHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEECCCHHHH
TIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRIAAHEIMLGTPAIRNLIRE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHEECCHHHHHHHHH
NKIAQMYSSIQTGAGDGMITLDQTLKNLVARGTISKDVARPYAKQPEAFL
HHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHHHCHHHCCCCCCC
>Mature Secondary Structure 
AEQNLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPDMSHKVVHQLIYDIM
CCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHH
NDRQRADFEEFFETDFSFEIPNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVF
CCCCCCCHHHHHCCCCCCCCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
KRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEHILTIEDPIEFVHESKKSLINQ
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHH
REVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAK
HHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEECCCHHHH
TIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRIAAHEIMLGTPAIRNLIRE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHEECCHHHHHHHHH
NKIAQMYSSIQTGAGDGMITLDQTLKNLVARGTISKDVARPYAKQPEAFL
HHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHHHCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]