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The map label for this gene is pilT [H]

Identifier: 93005123

GI number: 93005123

Start: 349971

End: 351092

Strand: Reverse

Name: pilT [H]

Synonym: Pcryo_0292

Alternate gene names: 93005123

Gene position: 351092-349971 (Counterclockwise)

Preceding gene: 93005124

Following gene: 93005121

Centisome position: 11.47

GC content: 43.76

Gene sequence:

>1122_bases
ATGGACATTGATAAACTTTTACAATTGATGATTAACAAAAACGGCTCTGACCTTTTTATCACTGCTGATGTCGCCCCTTC
CATGAAAATCAATGGCAAAATACTGCCGGTTGGTAAAACGCCTTTAACTGCTGAGCAGACTATGAGGCTGGTTAAAGGTG
TCATGACAGTCAATCAGCAAAAAGAGTTTGATGAGACCAACGAGTGCCAGTTTGCGATTACTGATCAGGCGCAACAAGCA
CGTTTTAGGGTCAGTGCCTTTATACAACGTGATATGGCAGGGATGATTTTACGGAAAATCGAAAACAAAATCCCAACGGT
TGAAGAGCTGCGCCTGCCACCAGCATTAAAAGAGCTGGCAATGAAGAAGCGCGGTATTATTTTGCTGGTTGGTGCAACTG
GTACGGGTAAATCAACCACCCTTGCAGCAATGATAGGACATCGCAATCAAAACTCTCACGGTCATATTATTACCATAGAA
GATCCTATCGAGTTTGTCCATAAGCATCGTGGCTGTATTATTACCCAGCGCGAAGTCGGTATCGATACCCACTCTTTTGA
AGCCGGTCTAAAAAACACCTTGCGCCAAGCGCCAGATGTGATTTTGATTGGTGAGATTCGCAATCGTGAAGTCATGAGTT
ACGCGATTCAATATGCTGAAACGGGGCATTTGGTATTTGCAACGTTGCACGCCAATAATGCCAACCAAGCAATCGACCGC
ATTATTCACTTTTTTGAGACCAGTCGCCATAATCAATTGTTTATGGATTTATCGCTTAACTTACAAGCCATTATCGCCCA
GCAGTTGATTCCAACGCCTGACGGCAAGGGACGCCGGGCTGCTATCGAAATCTTGCTAAACTCACAGCTGATCAGCGACT
ATATCCGCAAAGGTGAGGTGCACGAAATAAAAGATGTCATGACACGCTCACGTGATAGCGGTATGCAGACATTTGACCAA
GCACTGTTTGATCTATATGAAAACGGTGAGATTACTTATAAAGAAGCGATTCTTCATGCAGACTCGCCTAATGATCTGCG
CTTAAAAATCAAACTCAGCAGCAAAAATGGCGTGGCAAACTTAGATGAAGGCGCGGATAGTTTATCGTTAGACATTACTT
AA

Upstream 100 bases:

>100_bases
GCAATCATCCTTTAAGTTAACTGTCATTTTTCGCAAAAAAGTTTATTGAAGCAGGTTTCACTCTATTCATTGCACATTTT
CTCGCACATATAGGTACGTT

Downstream 100 bases:

>100_bases
TAATGTATTTTTAACCATAGTTATTATTTTTTATGCCTAAGAAACCGTTATAAAAAAGCCCTCATCATATATAAGGGCTT
TTTTAATTTTATCTCATCGA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTVNQQKEFDETNECQFAITDQAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNGVANLDEGADSLSLDIT

Sequences:

>Translated_373_residues
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTVNQQKEFDETNECQFAITDQAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNGVANLDEGADSLSLDIT
>Mature_373_residues
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTVNQQKEFDETNECQFAITDQAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNGVANLDEGADSLSLDIT

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=35.8255451713396, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789723, Length=155, Percent_Identity=42.5806451612903, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786296, Length=310, Percent_Identity=27.0967741935484, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41688; Mature: 41688

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTVNQQ
CCHHHHHHHHHCCCCCEEEEEECCCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCC
KEFDETNECQFAITDQAQQARFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHH
MKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIEDPIEFVHKHRGCIITQREVG
HHCCCEEEEEECCCCCCCHHHEEECCCCCCCCCCCEEEECCHHHHHHHCCCEEEEEHHCC
IDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
CCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHH
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEV
HHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCH
HEIKDVMTRSRDSGMQTFDQALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNGVAN
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEHEEEECCCCCCEEEEEEECCCCCCCC
LDEGADSLSLDIT
CCCCCCCEEEEEC
>Mature Secondary Structure
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTVNQQ
CCHHHHHHHHHCCCCCEEEEEECCCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCC
KEFDETNECQFAITDQAQQARFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHH
MKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIEDPIEFVHKHRGCIITQREVG
HHCCCEEEEEECCCCCCCHHHEEECCCCCCCCCCCEEEECCHHHHHHHCCCEEEEEHHCC
IDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
CCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHH
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEV
HHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCH
HEIKDVMTRSRDSGMQTFDQALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNGVAN
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEHEEEECCCCCCEEEEEEECCCCCCCC
LDEGADSLSLDIT
CCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]