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The map label for this gene is manB [H]

Identifier: 93004951

GI number: 93004951

Start: 142740

End: 144233

Strand: Reverse

Name: manB [H]

Synonym: Pcryo_0120

Alternate gene names: 93004951

Gene position: 144233-142740 (Counterclockwise)

Preceding gene: 93004960

Following gene: 93004937

Centisome position: 4.71

GC content: 44.18

Gene sequence:

>1494_bases
ATGCCCACATCCGATACGAATAATGCCAGCTATGCGACTCAAACCCAGTTCGCACCGATTAACATTGATTCATTTAAAGC
TTACGATATCCGCGGCGAGCTTGGTGTCAATCTCGATGAAGCTATCGCCTACCGTATCGGCCGTGCCTTTGCCCAAATTT
TATTCGAGCGTTATAACAGTGCCGATAATGATGCTACCGCTGAACTGAAAAACTTAAAATCAGCTATCGTTATTGGTAGC
GATATTCGCCATTCAAGTGAACAGTTAAAACAAGCGACTATCAAAGGCATGCTAGATGCTGGCGTCGATGTTATCGACTT
GGGAATGACAGGTACAGAAGAGGTGTATTTTGCCACCAGCTACTATCAAGCGTTGGGCGGTATCGAAGTCACTGCCAGTC
ATAATCCGATTAATTATAATGGCTTAAAATTGGTCAAAGAGCACTCAAAACCGATTAGCGCCGATGATGGTTTGGCAGAG
ATTCAAGCGTTGGCGGAATCGGGGCAATTTACCACTGCTAACACCTTAGGCAACCTACAATTACTGACGGATAAAAGCGC
CTATATCAATCATGTGATGACTTTCATCGATACTGACAAATTAAAACCACTTAAACTGGTTATTAATTCAGGCAATGGCA
GCGCCGGTCCCGTGGTTGATTTATTAATCGAAAAGTTAGCACAAGCTGGCGCACCGATTGAAGTCATTAAACTGCATCAT
GCCCCTGACGGTAGTTTTCCAAATGGCATCCCTAATCCGATGATTTTGGCTAATAGAACCGCCACCCAGCAAGCGGTGTT
AGAAAATAAAGCCGACCTTGGGATTGCCTTTGATGGCGATTTTGACCGCTGCTTTTTATTCGATGAATACGGCGAATTTA
TCGATGGTAGCTATGTCGTTGGGATGCTGGCGCAAGCCTTTTTAAATAAGTATGCAGGCGAGTCGATTGTTTATGATCCA
CGGGTGATTTATAACACCGAAGCCGTCATCCATGAGCATAACGGTAAGGCGGTTATTAGTAAATCTGGGCACTCATTTAT
CAAGCAGGTGATGCGTGATTCTGGTGCGGTTTATGGTGGCGAGATGTCAGCGCATCATTATTTTCGCGATTTTTTCTACT
GCGATAGCGGTATGATTCCTTGGCTGCTGACCATTGAGCTATTATCGATTACCGGTAAAACCTTGTCAGAATTGGTCAGT
GGTTATATTGCAGCCTATCCAAGCTCAGGCGAGATGAACTTTCATCTGACCACGCATGATGCGCAGACGATTATTAGCGC
TATCGAAGAAAAGTTTAGTACCGAAAACCCAACCAAATCGATGCTCGATGGTTTGAGTTTAAACTTTGGCGAATGGCGTT
TTAACTTACGCGCCTCAAATACTGAACCATTAGTTCGGTTAAATATTGAAAGTCGCGGTGATGAAGATTTACTACAGACC
AAGACCCAGGAGATTCAACAATGGCTTGCCAGTAAAGGTGCTGTACCTGCTTAA

Upstream 100 bases:

>100_bases
ACGATTTTGTCGTTAGTGTAGCATTGAACACTGATTAGCTTATAATTGTGTAACCTACTTAATTATCATCTCACTGCTTA
AACGCAAGGATTTTATCGTT

Downstream 100 bases:

>100_bases
CTTCAATAGCTGCAATGCTAAATAAAGACATTCTAAACTTATAAAGATAAAAAAAGCGCCGATTAATTTTTATATTAATC
GGCGCTTTTATTTAGCTTTA

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MPTSDTNNASYATQTQFAPINIDSFKAYDIRGELGVNLDEAIAYRIGRAFAQILFERYNSADNDATAELKNLKSAIVIGS
DIRHSSEQLKQATIKGMLDAGVDVIDLGMTGTEEVYFATSYYQALGGIEVTASHNPINYNGLKLVKEHSKPISADDGLAE
IQALAESGQFTTANTLGNLQLLTDKSAYINHVMTFIDTDKLKPLKLVINSGNGSAGPVVDLLIEKLAQAGAPIEVIKLHH
APDGSFPNGIPNPMILANRTATQQAVLENKADLGIAFDGDFDRCFLFDEYGEFIDGSYVVGMLAQAFLNKYAGESIVYDP
RVIYNTEAVIHEHNGKAVISKSGHSFIKQVMRDSGAVYGGEMSAHHYFRDFFYCDSGMIPWLLTIELLSITGKTLSELVS
GYIAAYPSSGEMNFHLTTHDAQTIISAIEEKFSTENPTKSMLDGLSLNFGEWRFNLRASNTEPLVRLNIESRGDEDLLQT
KTQEIQQWLASKGAVPA

Sequences:

>Translated_497_residues
MPTSDTNNASYATQTQFAPINIDSFKAYDIRGELGVNLDEAIAYRIGRAFAQILFERYNSADNDATAELKNLKSAIVIGS
DIRHSSEQLKQATIKGMLDAGVDVIDLGMTGTEEVYFATSYYQALGGIEVTASHNPINYNGLKLVKEHSKPISADDGLAE
IQALAESGQFTTANTLGNLQLLTDKSAYINHVMTFIDTDKLKPLKLVINSGNGSAGPVVDLLIEKLAQAGAPIEVIKLHH
APDGSFPNGIPNPMILANRTATQQAVLENKADLGIAFDGDFDRCFLFDEYGEFIDGSYVVGMLAQAFLNKYAGESIVYDP
RVIYNTEAVIHEHNGKAVISKSGHSFIKQVMRDSGAVYGGEMSAHHYFRDFFYCDSGMIPWLLTIELLSITGKTLSELVS
GYIAAYPSSGEMNFHLTTHDAQTIISAIEEKFSTENPTKSMLDGLSLNFGEWRFNLRASNTEPLVRLNIESRGDEDLLQT
KTQEIQQWLASKGAVPA
>Mature_496_residues
PTSDTNNASYATQTQFAPINIDSFKAYDIRGELGVNLDEAIAYRIGRAFAQILFERYNSADNDATAELKNLKSAIVIGSD
IRHSSEQLKQATIKGMLDAGVDVIDLGMTGTEEVYFATSYYQALGGIEVTASHNPINYNGLKLVKEHSKPISADDGLAEI
QALAESGQFTTANTLGNLQLLTDKSAYINHVMTFIDTDKLKPLKLVINSGNGSAGPVVDLLIEKLAQAGAPIEVIKLHHA
PDGSFPNGIPNPMILANRTATQQAVLENKADLGIAFDGDFDRCFLFDEYGEFIDGSYVVGMLAQAFLNKYAGESIVYDPR
VIYNTEAVIHEHNGKAVISKSGHSFIKQVMRDSGAVYGGEMSAHHYFRDFFYCDSGMIPWLLTIELLSITGKTLSELVSG
YIAAYPSSGEMNFHLTTHDAQTIISAIEEKFSTENPTKSMLDGLSLNFGEWRFNLRASNTEPLVRLNIESRGDEDLLQTK
TQEIQQWLASKGAVPA

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=472, Percent_Identity=52.9661016949153, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1789566, Length=405, Percent_Identity=28.1481481481481, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 54351; Mature: 54220

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSDTNNASYATQTQFAPINIDSFKAYDIRGELGVNLDEAIAYRIGRAFAQILFERYNS
CCCCCCCCCCCEECCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
ADNDATAELKNLKSAIVIGSDIRHSSEQLKQATIKGMLDAGVDVIDLGMTGTEEVYFATS
CCCHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEHH
YYQALGGIEVTASHNPINYNGLKLVKEHSKPISADDGLAEIQALAESGQFTTANTLGNLQ
HHHHHCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCEE
LLTDKSAYINHVMTFIDTDKLKPLKLVINSGNGSAGPVVDLLIEKLAQAGAPIEVIKLHH
EEECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEE
APDGSFPNGIPNPMILANRTATQQAVLENKADLGIAFDGDFDRCFLFDEYGEFIDGSYVV
CCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHHHHCCHHHH
GMLAQAFLNKYAGESIVYDPRVIYNTEAVIHEHNGKAVISKSGHSFIKQVMRDSGAVYGG
HHHHHHHHHHHCCCCEEECCEEEEECEEEEEECCCCEEEECCCHHHHHHHHHHCCCEECC
EMSAHHYFRDFFYCDSGMIPWLLTIELLSITGKTLSELVSGYIAAYPSSGEMNFHLTTHD
CCHHHHHHHHHEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCEEEEEECCH
AQTIISAIEEKFSTENPTKSMLDGLSLNFGEWRFNLRASNTEPLVRLNIESRGDEDLLQT
HHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHH
KTQEIQQWLASKGAVPA
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PTSDTNNASYATQTQFAPINIDSFKAYDIRGELGVNLDEAIAYRIGRAFAQILFERYNS
CCCCCCCCCCEECCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
ADNDATAELKNLKSAIVIGSDIRHSSEQLKQATIKGMLDAGVDVIDLGMTGTEEVYFATS
CCCHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEHH
YYQALGGIEVTASHNPINYNGLKLVKEHSKPISADDGLAEIQALAESGQFTTANTLGNLQ
HHHHHCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCEE
LLTDKSAYINHVMTFIDTDKLKPLKLVINSGNGSAGPVVDLLIEKLAQAGAPIEVIKLHH
EEECCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEE
APDGSFPNGIPNPMILANRTATQQAVLENKADLGIAFDGDFDRCFLFDEYGEFIDGSYVV
CCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHHHHCCHHHH
GMLAQAFLNKYAGESIVYDPRVIYNTEAVIHEHNGKAVISKSGHSFIKQVMRDSGAVYGG
HHHHHHHHHHHCCCCEEECCEEEEECEEEEEECCCCEEEECCCHHHHHHHHHHCCCEECC
EMSAHHYFRDFFYCDSGMIPWLLTIELLSITGKTLSELVSGYIAAYPSSGEMNFHLTTHD
CCHHHHHHHHHEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCEEEEEECCH
AQTIISAIEEKFSTENPTKSMLDGLSLNFGEWRFNLRASNTEPLVRLNIESRGDEDLLQT
HHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHH
KTQEIQQWLASKGAVPA
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1383393 [H]