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The map label for this gene is gltA [H]

Identifier: 93004937

GI number: 93004937

Start: 122199

End: 123479

Strand: Reverse

Name: gltA [H]

Synonym: Pcryo_0106

Alternate gene names: 93004937

Gene position: 123479-122199 (Counterclockwise)

Preceding gene: 93004951

Following gene: 93004934

Centisome position: 4.04

GC content: 44.11

Gene sequence:

>1281_bases
ATGGCTGATAATCAAGCCACATTGACCGTAGATGGTAAGGACTACCAACTTCCTATTACTGAGGGTACTTTAGGGCCGTC
AGTTATCGAAGTTGCTGCTTTCCAACAAGCTGGCTACTGGACCTATGATCCAGGCTTTATGGCAACTGCTCCTGTCGAAT
CGAAAATTACCTTCATTGATGGCGGTAAAGGCGAGCTATTGCATCGCGGCTATCCTATTGACCAGTTAGCAAACAATGCA
GAATATCTAGAAGTGGCTTATGCACTGATTCATGGTGATCTGCCTAACGCTGAGCAAAAAGCCGATTTCTTTGAAAAAAT
TCGTAAGCATACTGGTGTTCATGATCAATTGCGTAAGTTCTTTGAAGGCTTCCGTCGTGACGCCCATCCAATGGCAATTA
TGGTTGGTGTCGTTGGTGCCTTATCTGCGTTTTATCATGAAGATTTAGACATCAGCAATGAAGAACATCGTGAAATTACC
GCGATTCGTCTCATCGCTAAAATGCCTACACTTGCGGCAATGAGTTATAAATACTCACAAGGCGAACCGTTCATGTATCC
ACGTAATGATTTCAATTATGCGGAAAACTTCTTATACATGATGTTTGCAACCCCTGCTGATGTTGATTATAAAACCAACG
ACGTTATCACGCGTGCTATGGACAAAATCTTTACCTTGCATGCTGACCATGAGCAAAACGCATCAACGTCTACCGTACGT
CTAGCAGGTTCTACTGGCGCCAATCCTTACGCTTGTATCGCTGCTGGTATCGCCGCCCTTTGGGGACCATCACATGGCGG
CGCCAATGAAGCCGTACTTGAGATGCTAGATGAAATCGGTTCAGTAGAAAACGTTCCAGAATTTATGGAAAAAGTGAAAA
GCCGTGAAGTGAAACTGATGGGCTTTGGTCACCGTGTTTACAAAAACTTTGACCCACGCGCACAAGTGATGAAAGAGACT
TGTGACGAAGTACTAGGCGCATTAGGTATCAATGATCCTAAGCTTGAGCTTGCTATGGCACTTGAGAAAATCGCTTTAGA
AGACCCGTTCTTCGTTGAGCGTAACTTGTATCCAAACGTTGATTTTTACTCTGGTATTATCCTTAAAGCCATCGGTATCC
CAACGTCAATGTTTACCGTTATCTTTTCACTAGCTCGTACTTCTGGTTGGATCAGCCATTGGTTAGAGATGCACAGCGCA
CCTTTCAAAATCGGTCGCCCACGTCAGTTATACACTGGTGAAACGCAGCGCGATTTTGTTAAAGTTGAAGATCGTAAATA
G

Upstream 100 bases:

>100_bases
AATAGGTCACATTCATTTTGGCCATTTGACTATTTATGACGCGAGGGTGTGCGTCACTATTATTTTTGTTCTAACAAGTT
AAAACAATGGAGATTAAATT

Downstream 100 bases:

>100_bases
TAATTAAGCATGTGTAAAAGTAAACAGTCATAAAAAATCTCGCCATGTGCGAGATTTTTTTATGGCTAAATTTAACGGAT
TACTTAAACTCTTTTAACGT

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MADNQATLTVDGKDYQLPITEGTLGPSVIEVAAFQQAGYWTYDPGFMATAPVESKITFIDGGKGELLHRGYPIDQLANNA
EYLEVAYALIHGDLPNAEQKADFFEKIRKHTGVHDQLRKFFEGFRRDAHPMAIMVGVVGALSAFYHEDLDISNEEHREIT
AIRLIAKMPTLAAMSYKYSQGEPFMYPRNDFNYAENFLYMMFATPADVDYKTNDVITRAMDKIFTLHADHEQNASTSTVR
LAGSTGANPYACIAAGIAALWGPSHGGANEAVLEMLDEIGSVENVPEFMEKVKSREVKLMGFGHRVYKNFDPRAQVMKET
CDEVLGALGINDPKLELAMALEKIALEDPFFVERNLYPNVDFYSGIILKAIGIPTSMFTVIFSLARTSGWISHWLEMHSA
PFKIGRPRQLYTGETQRDFVKVEDRK

Sequences:

>Translated_426_residues
MADNQATLTVDGKDYQLPITEGTLGPSVIEVAAFQQAGYWTYDPGFMATAPVESKITFIDGGKGELLHRGYPIDQLANNA
EYLEVAYALIHGDLPNAEQKADFFEKIRKHTGVHDQLRKFFEGFRRDAHPMAIMVGVVGALSAFYHEDLDISNEEHREIT
AIRLIAKMPTLAAMSYKYSQGEPFMYPRNDFNYAENFLYMMFATPADVDYKTNDVITRAMDKIFTLHADHEQNASTSTVR
LAGSTGANPYACIAAGIAALWGPSHGGANEAVLEMLDEIGSVENVPEFMEKVKSREVKLMGFGHRVYKNFDPRAQVMKET
CDEVLGALGINDPKLELAMALEKIALEDPFFVERNLYPNVDFYSGIILKAIGIPTSMFTVIFSLARTSGWISHWLEMHSA
PFKIGRPRQLYTGETQRDFVKVEDRK
>Mature_425_residues
ADNQATLTVDGKDYQLPITEGTLGPSVIEVAAFQQAGYWTYDPGFMATAPVESKITFIDGGKGELLHRGYPIDQLANNAE
YLEVAYALIHGDLPNAEQKADFFEKIRKHTGVHDQLRKFFEGFRRDAHPMAIMVGVVGALSAFYHEDLDISNEEHREITA
IRLIAKMPTLAAMSYKYSQGEPFMYPRNDFNYAENFLYMMFATPADVDYKTNDVITRAMDKIFTLHADHEQNASTSTVRL
AGSTGANPYACIAAGIAALWGPSHGGANEAVLEMLDEIGSVENVPEFMEKVKSREVKLMGFGHRVYKNFDPRAQVMKETC
DEVLGALGINDPKLELAMALEKIALEDPFFVERNLYPNVDFYSGIILKAIGIPTSMFTVIFSLARTSGWISHWLEMHSAP
FKIGRPRQLYTGETQRDFVKVEDRK

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=350, Percent_Identity=28, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1786939, Length=422, Percent_Identity=62.3222748815166, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1786527, Length=369, Percent_Identity=33.6043360433604, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17555174, Length=349, Percent_Identity=28.080229226361, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6319850, Length=360, Percent_Identity=25.5555555555556, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=362, Percent_Identity=25.9668508287293, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325257, Length=356, Percent_Identity=26.9662921348315, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24640126, Length=349, Percent_Identity=27.7936962750716, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640124, Length=349, Percent_Identity=27.5071633237822, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356863, Length=353, Percent_Identity=24.929178470255, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47711; Mature: 47580

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNQATLTVDGKDYQLPITEGTLGPSVIEVAAFQQAGYWTYDPGFMATAPVESKITFID
CCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCEEECCCCCEEEEEE
GGKGELLHRGYPIDQLANNAEYLEVAYALIHGDLPNAEQKADFFEKIRKHTGVHDQLRKF
CCCCCEEECCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
FEGFRRDAHPMAIMVGVVGALSAFYHEDLDISNEEHREITAIRLIAKMPTLAAMSYKYSQ
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHEEECCC
GEPFMYPRNDFNYAENFLYMMFATPADVDYKTNDVITRAMDKIFTLHADHEQNASTSTVR
CCCCCCCCCCCCHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCEEE
LAGSTGANPYACIAAGIAALWGPSHGGANEAVLEMLDEIGSVENVPEFMEKVKSREVKLM
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEE
GFGHRVYKNFDPRAQVMKETCDEVLGALGINDPKLELAMALEKIALEDPFFVERNLYPNV
ECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCC
DFYSGIILKAIGIPTSMFTVIFSLARTSGWISHWLEMHSAPFKIGRPRQLYTGETQRDFV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
KVEDRK
EECCCC
>Mature Secondary Structure 
ADNQATLTVDGKDYQLPITEGTLGPSVIEVAAFQQAGYWTYDPGFMATAPVESKITFID
CCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCEEECCCCCEEEEEE
GGKGELLHRGYPIDQLANNAEYLEVAYALIHGDLPNAEQKADFFEKIRKHTGVHDQLRKF
CCCCCEEECCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
FEGFRRDAHPMAIMVGVVGALSAFYHEDLDISNEEHREITAIRLIAKMPTLAAMSYKYSQ
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHEEECCC
GEPFMYPRNDFNYAENFLYMMFATPADVDYKTNDVITRAMDKIFTLHADHEQNASTSTVR
CCCCCCCCCCCCHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCEEE
LAGSTGANPYACIAAGIAALWGPSHGGANEAVLEMLDEIGSVENVPEFMEKVKSREVKLM
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEE
GFGHRVYKNFDPRAQVMKETCDEVLGALGINDPKLELAMALEKIALEDPFFVERNLYPNV
ECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCC
DFYSGIILKAIGIPTSMFTVIFSLARTSGWISHWLEMHSAPFKIGRPRQLYTGETQRDFV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
KVEDRK
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3442626 [H]