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The map label for this gene is pycB [H]

Identifier: 92113680

GI number: 92113680

Start: 1769223

End: 1771037

Strand: Direct

Name: pycB [H]

Synonym: Csal_1556

Alternate gene names: 92113680

Gene position: 1769223-1771037 (Clockwise)

Preceding gene: 92113679

Following gene: 92113684

Centisome position: 47.86

GC content: 65.45

Gene sequence:

>1815_bases
ATGAATGCCGTGTCATCTTCCCAGGTCAAAATCACCGATGTCGTGCTGCGCGACGGCCACCAGTCCTTGATCGCCACGCG
CCTGCGCACCGAGGACATGCTGCCCGCCTGTGCCAAGCTGGATGCGATCGGCTATCACTCGCTCGAGGTATGGGGCGGTG
CGACCTTCGATGCCTGCGTGCGCTTTCTCAAGGAAGATCCCTGGGAGCGCCTGCGCGCCTTCAAGGAGGCGATGCCCAAC
ACGCCGCTGCAGATGTTGCTGCGCGGGCAGAACCTGCTGGGGTATCGGCATTACGCCGATGACGTCGTCGAGCGTTTCGT
CGCCCGGTCGGCGGACAACGGCGTCGATGTCTTCCGCGTCTTCGATGCGCTCAACGACCTGCGCAACCTGGAAACCGCCA
TGCGTGCGGTCAAGGCCAGCGGCAAGCACGCCCAGGGCACGATCTGCTATACGGTGAGCCCGGTGCATACCCTGGCGATG
TACGTGGATCAGGCCAAGCGACTGGTCGACATGGGCGCCGATTCGATCGCCATCAAGGACATGGCGGGGCTGCTGACGCC
GTATGCCACGAGCGAGCTGGTGGCCGCCCTGGTCGAGGCCGTTGAGGTGCCGGTTCACCTGCATGCCCATGCCACCTCTG
GATTGGCGCCGCTTTGCCATCTCAAGGCGGTCGAGGCGGGCTGCCGACACATCGATACCTGCATCTCGGCATTCGCCGGG
GGGACCAGTCATCCCTCGACCGAATCCATGGTCGCCGCCTTCCAGGGCACCGAGTACGACAGCGGCCTGGACCTCGAGGC
CCTCAAGGACATCGGTGACTATTTCCGCGGCGTGCGACAGAAGTATGCCGCCTTCGAAAGCGAGTTCACCCGCGAGGACG
TCTCGGTACAGATCAACCAGGTACCGGGCGGCATGATGTCCAACCTCGCCAATCAGCTCAAGGAGCAGAACGCGCTTTCC
AAGATCCGCGACGTGTTCGACGAGATTCCGCGGGTGCGCGCCGATCTCGGCTATCCGCCCCTGGTCACGCCGACCTCGCA
GATCGTCGGTACCCAGGCCGTGATGAACGTTCTCACCGGCGAGCGCTACAAGACCATCACCAACGAAGTGAAGCGTTATC
TGCAGGGTGGGTACGGCCATCCGCCGGCGGCGGTGGATGCCGACGTGCGCCGTCAGGCCATCGGCAACTCGCCGGTGGAG
GAAGGGCGCCCCGCGGATCGGCTGTCTCCCGAGATGACGCGTCTGACGAAGGAGGTCGGCGAGCTGGCGGACAGCGAGGA
AGACGTGCTGACCTTCGCCATGTTCCCCGAGCTGGGACGTGATTACCTCCAGGGACGGCGCGACGGTACGCTGGTCACGG
AGCCGATACCCGCCGCCGCCGCCGGCGATGCCAATCGGCCGGTCACCGAAGGGGTGCCCACCGAGTTCGTGATCGACGTG
CACGGGGAGTCCTATGAAGTGCAGATCACCGGGGTCGGCGGCAACAGCGGTGGCAAGCGTCAGGTGTATCTGACGCTCGA
CGGCATGCCGGAGGAGGTCGTCTTCGAGGCCAAGGATGCCTTCGTCGGCGGCGAGACCTCGGGGCGTGCGCGTGCTTCGC
AGCCCGGCCACGTCACCACTTCCATGCCCGGCAACATCGTCGATGTGCTGGTCGCCGAGGGGGATCGCGTCGAGGAAGGC
CAGGCGGTCTTGATCACCGAGGCGATGAAGATGGAGACCGAGGTGCAGGCGCGGGTCGCGGGGACCGTCGAGGCGGTACA
CGTCGCCAAGGGCGACCGTGTCACGCCGGGCGAGGTACTGGTCGAGATCGCCTGA

Upstream 100 bases:

>100_bases
CCGAATTGCTCAATTACTCGGTCAAGCGCAATCCCGAGGAAGTCGCCCTGGCCATTGCCGCCGCCATCGCCGCCCACGCC
GGTCTTTAAGGAGATACCTC

Downstream 100 bases:

>100_bases
TCCGCTGCCGCCGCGAGCGCTTCGCCCCGCCATCCGCGATGTTTCCCCGTCGGGAAGCGTTCGCGGTGGCGGGGCGATGT
CGTGCTAGGCCGCCGCTCGC

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MNAVSSSQVKITDVVLRDGHQSLIATRLRTEDMLPACAKLDAIGYHSLEVWGGATFDACVRFLKEDPWERLRAFKEAMPN
TPLQMLLRGQNLLGYRHYADDVVERFVARSADNGVDVFRVFDALNDLRNLETAMRAVKASGKHAQGTICYTVSPVHTLAM
YVDQAKRLVDMGADSIAIKDMAGLLTPYATSELVAALVEAVEVPVHLHAHATSGLAPLCHLKAVEAGCRHIDTCISAFAG
GTSHPSTESMVAAFQGTEYDSGLDLEALKDIGDYFRGVRQKYAAFESEFTREDVSVQINQVPGGMMSNLANQLKEQNALS
KIRDVFDEIPRVRADLGYPPLVTPTSQIVGTQAVMNVLTGERYKTITNEVKRYLQGGYGHPPAAVDADVRRQAIGNSPVE
EGRPADRLSPEMTRLTKEVGELADSEEDVLTFAMFPELGRDYLQGRRDGTLVTEPIPAAAAGDANRPVTEGVPTEFVIDV
HGESYEVQITGVGGNSGGKRQVYLTLDGMPEEVVFEAKDAFVGGETSGRARASQPGHVTTSMPGNIVDVLVAEGDRVEEG
QAVLITEAMKMETEVQARVAGTVEAVHVAKGDRVTPGEVLVEIA

Sequences:

>Translated_604_residues
MNAVSSSQVKITDVVLRDGHQSLIATRLRTEDMLPACAKLDAIGYHSLEVWGGATFDACVRFLKEDPWERLRAFKEAMPN
TPLQMLLRGQNLLGYRHYADDVVERFVARSADNGVDVFRVFDALNDLRNLETAMRAVKASGKHAQGTICYTVSPVHTLAM
YVDQAKRLVDMGADSIAIKDMAGLLTPYATSELVAALVEAVEVPVHLHAHATSGLAPLCHLKAVEAGCRHIDTCISAFAG
GTSHPSTESMVAAFQGTEYDSGLDLEALKDIGDYFRGVRQKYAAFESEFTREDVSVQINQVPGGMMSNLANQLKEQNALS
KIRDVFDEIPRVRADLGYPPLVTPTSQIVGTQAVMNVLTGERYKTITNEVKRYLQGGYGHPPAAVDADVRRQAIGNSPVE
EGRPADRLSPEMTRLTKEVGELADSEEDVLTFAMFPELGRDYLQGRRDGTLVTEPIPAAAAGDANRPVTEGVPTEFVIDV
HGESYEVQITGVGGNSGGKRQVYLTLDGMPEEVVFEAKDAFVGGETSGRARASQPGHVTTSMPGNIVDVLVAEGDRVEEG
QAVLITEAMKMETEVQARVAGTVEAVHVAKGDRVTPGEVLVEIA
>Mature_604_residues
MNAVSSSQVKITDVVLRDGHQSLIATRLRTEDMLPACAKLDAIGYHSLEVWGGATFDACVRFLKEDPWERLRAFKEAMPN
TPLQMLLRGQNLLGYRHYADDVVERFVARSADNGVDVFRVFDALNDLRNLETAMRAVKASGKHAQGTICYTVSPVHTLAM
YVDQAKRLVDMGADSIAIKDMAGLLTPYATSELVAALVEAVEVPVHLHAHATSGLAPLCHLKAVEAGCRHIDTCISAFAG
GTSHPSTESMVAAFQGTEYDSGLDLEALKDIGDYFRGVRQKYAAFESEFTREDVSVQINQVPGGMMSNLANQLKEQNALS
KIRDVFDEIPRVRADLGYPPLVTPTSQIVGTQAVMNVLTGERYKTITNEVKRYLQGGYGHPPAAVDADVRRQAIGNSPVE
EGRPADRLSPEMTRLTKEVGELADSEEDVLTFAMFPELGRDYLQGRRDGTLVTEPIPAAAAGDANRPVTEGVPTEFVIDV
HGESYEVQITGVGGNSGGKRQVYLTLDGMPEEVVFEAKDAFVGGETSGRARASQPGHVTTSMPGNIVDVLVAEGDRVEEG
QAVLITEAMKMETEVQARVAGTVEAVHVAKGDRVTPGEVLVEIA

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=622, Percent_Identity=34.4051446945338, Blast_Score=315, Evalue=1e-85,
Organism=Homo sapiens, GI106049295, Length=622, Percent_Identity=34.4051446945338, Blast_Score=315, Evalue=1e-85,
Organism=Homo sapiens, GI106049292, Length=622, Percent_Identity=34.4051446945338, Blast_Score=315, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17562816, Length=627, Percent_Identity=35.2472089314195, Blast_Score=340, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6321376, Length=622, Percent_Identity=33.1189710610932, Blast_Score=309, Evalue=7e-85,
Organism=Saccharomyces cerevisiae, GI6319695, Length=622, Percent_Identity=32.3151125401929, Blast_Score=300, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24652212, Length=633, Percent_Identity=34.4391785150079, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24652210, Length=633, Percent_Identity=34.4391785150079, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24652214, Length=633, Percent_Identity=34.4391785150079, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI19921944, Length=633, Percent_Identity=34.4391785150079, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24652216, Length=633, Percent_Identity=34.4391785150079, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI281363050, Length=633, Percent_Identity=34.4391785150079, Blast_Score=315, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24652224, Length=633, Percent_Identity=34.4391785150079, Blast_Score=315, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24652222, Length=633, Percent_Identity=34.4391785150079, Blast_Score=315, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24652220, Length=633, Percent_Identity=34.4391785150079, Blast_Score=315, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24652218, Length=633, Percent_Identity=34.4391785150079, Blast_Score=315, Evalue=8e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 65339; Mature: 65339

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00165 DEHYDRATASE_SER_THR ; PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVSSSQVKITDVVLRDGHQSLIATRLRTEDMLPACAKLDAIGYHSLEVWGGATFDACV
CCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
RFLKEDPWERLRAFKEAMPNTPLQMLLRGQNLLGYRHYADDVVERFVARSADNGVDVFRV
HHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FDALNDLRNLETAMRAVKASGKHAQGTICYTVSPVHTLAMYVDQAKRLVDMGADSIAIKD
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHH
MAGLLTPYATSELVAALVEAVEVPVHLHAHATSGLAPLCHLKAVEAGCRHIDTCISAFAG
HHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GTSHPSTESMVAAFQGTEYDSGLDLEALKDIGDYFRGVRQKYAAFESEFTREDVSVQINQ
CCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
VPGGMMSNLANQLKEQNALSKIRDVFDEIPRVRADLGYPPLVTPTSQIVGTQAVMNVLTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
ERYKTITNEVKRYLQGGYGHPPAAVDADVRRQAIGNSPVEEGRPADRLSPEMTRLTKEVG
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHCCCCCHHCCHHHHHHHHHHH
ELADSEEDVLTFAMFPELGRDYLQGRRDGTLVTEPIPAAAAGDANRPVTEGVPTEFVIDV
HHCCCCHHHEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEE
HGESYEVQITGVGGNSGGKRQVYLTLDGMPEEVVFEAKDAFVGGETSGRARASQPGHVTT
CCCEEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCEECCCCCCCCCCCCCCEEEC
SMPGNIVDVLVAEGDRVEEGQAVLITEAMKMETEVQARVAGTVEAVHVAKGDRVTPGEVL
CCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEE
VEIA
EEEC
>Mature Secondary Structure
MNAVSSSQVKITDVVLRDGHQSLIATRLRTEDMLPACAKLDAIGYHSLEVWGGATFDACV
CCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
RFLKEDPWERLRAFKEAMPNTPLQMLLRGQNLLGYRHYADDVVERFVARSADNGVDVFRV
HHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FDALNDLRNLETAMRAVKASGKHAQGTICYTVSPVHTLAMYVDQAKRLVDMGADSIAIKD
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHH
MAGLLTPYATSELVAALVEAVEVPVHLHAHATSGLAPLCHLKAVEAGCRHIDTCISAFAG
HHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GTSHPSTESMVAAFQGTEYDSGLDLEALKDIGDYFRGVRQKYAAFESEFTREDVSVQINQ
CCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
VPGGMMSNLANQLKEQNALSKIRDVFDEIPRVRADLGYPPLVTPTSQIVGTQAVMNVLTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
ERYKTITNEVKRYLQGGYGHPPAAVDADVRRQAIGNSPVEEGRPADRLSPEMTRLTKEVG
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHCCCCCHHCCHHHHHHHHHHH
ELADSEEDVLTFAMFPELGRDYLQGRRDGTLVTEPIPAAAAGDANRPVTEGVPTEFVIDV
HHCCCCHHHEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEE
HGESYEVQITGVGGNSGGKRQVYLTLDGMPEEVVFEAKDAFVGGETSGRARASQPGHVTT
CCCEEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCEECCCCCCCCCCCCCCEEEC
SMPGNIVDVLVAEGDRVEEGQAVLITEAMKMETEVQARVAGTVEAVHVAKGDRVTPGEVL
CCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEE
VEIA
EEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]