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The map label for this gene is topA [H]

Identifier: 92113684

GI number: 92113684

Start: 1775773

End: 1778406

Strand: Direct

Name: topA [H]

Synonym: Csal_1560

Alternate gene names: 92113684

Gene position: 1775773-1778406 (Clockwise)

Preceding gene: 92113680

Following gene: 92113685

Centisome position: 48.04

GC content: 64.01

Gene sequence:

>2634_bases
ATGGGTAAGTCACTGGTCATCGTGGAGTCGCCGGCCAAGGCCAAGACGATCAACAAGTATCTCGGCAACGATTTCGTCGT
GAAATCGAGCGTGGGACACATCCGTGACTTGCCGACGAGCGGATCCGGCAAGAACGCAACCGATCCCAAGGAGCGGGCAC
GCCAGGCGGCGGCGACACGCAAGATGTCGCCCGACGAGAAGGCCGTCTACAAGAAGCAGAAACACTGGAACCAGCTCGTG
CGCCGCATGGGGATCGACCCCGAGCACGGCTGGGAAGCCAACTACGAAGTGCTGCCGGGCAAGGAGAAGGTCGTCGCCGA
GCTCAAGAAGCTCGCCGAGAAGGCCGATGCCGTCTATCTCGCGACCGACCTTGACCGCGAGGGGGAGGCGATCGCCTGGC
ACTTGCAGGAGACCATCGGCGGCGATGCCTCGCGCTATCGGCGGGTGGTCTTCAACGAGATCACCAAGAACGCCATTCAG
GAGGCCTTCAAGACGCCGGGCGACCTCGACCTGTCCCGGGTCGAGGCCCAGCAGGCGCGTCGTTTCCTGGACCGGGTGGT
GGGCTTCATGCTCTCGCCGCTGTTGTGGAGCAAGATCGCTCGTGGCCTGTCGGCGGGGCGCGTGCAGTCGGTGGCGGTGC
GTCTGATCGTCGAGCGTGAGCGCGAAATTCGCGCCTTCGTGCCGGAGGAGTTCTGGGACGTTCACGCCGACCTCCTGCCC
GCCTCGGGCGAGGACACTCCCATCCGGTTCGAGGTGGCGCGTCACCAGGGCAAGGCGTTCCGCCCGACGTCCGAGCAGGA
AACGCTCGAGCGCATCGCGCCGCTGCGCGACGCAGCGCTGACGATCACCGAGCGCGAGACCAAGCCGACGCGCTCCAAGC
CCAACGCTCCCTTCATTACCTCGACGCTGCAGCAGGCCGCCAGCGGTCGGCTGGGCTTTTCGGTGAAGAAGACCATGACC
CTGGCGCAGCGTCTCTACGAGGCGGGGTACATCACCTACATGCGTACCGATTCCACCAACCTGTCCCAGGATGCGGTGGA
AAGTGCGCGTGCCTTCATCGGCGAGCGCTTCGGCCAGTCCTATCTGCCCGACGCTCCCAATCGCTACGCCAGCAAGGATG
GCGCTCAGGAAGCGCACGAGGCGATCCGTCCCACCGAGGTGACGCAGGCCGGCGACGACCTGGCGGTGGAGCGCGACGCC
CAGCGCTTGTACGAATTGATCTGGCGCCAGTTCGTGGCCTGCCAGATGGTGCCGGCGGAGTATCTGGCGACGACCTTGAC
CATCGAGGCCGAGGGCTTCGAGCTCAAGGCGCGCGGCCGCGTGCTCAAGTTCGATGGCTACACGCGGGTGATGAAGCCTG
CGGGCAAGAAAGAGGAAGACCAGTCGCTCCCCGACCTCGAGGAAGGGGCCGAGATGACCTTGCAGGCGCTCGATCCGCAG
CAGCACTTCACCAAGCCGGCTCCCCGCTATACCGAGGCGAGCCTGGTCAAGGAACTGGAAAAGCGCGGGATCGGGCGTCC
GTCGACCTATGCGTCGATCATTTCCACGATCCAGGACCGTGGCTACGTCAAGCTGGAAAATCGCCGCTTCTACGCCGAAA
AACTCGGCGATATCGTTACCGATCGCCTCGCCGAGTCGTTCAGCGATCTCATGGATTATGGCTTCACGGCGCGCATGGAA
GACCATCTCGACGAGATCGCCGCCGGCAATCGTGAATGGCGCGAGTTGCTGGATGCCTTCTACGCCGAGTTCAAGGCCAA
GCTGGAGCATGCCGAGGCCGAGGAGGGCATGCGTCCCAATCAGCCGGTGCCCACGGATATCGACTGTCCCACCTGCGGTC
GCAAGATGCAGATCCGCACGGCCTCCACGGGCGTTTTCCTGGGCTGCTCGGGGTATAACCTGCCGCCCAAGGAGCGCTGC
AAGACGACCATCGACCTCATTGCCGGTGACGAAGCGGTGGCGGCCGACGCCGACGAGGAAGCGGAAACCGAAGCGCTGCG
TGCCAAGCGGCGTTGCCCGGAGTGCGGTACTGCCATGGACAGCTACCTGATCGACGAGACCCGCAAGCTGCATATCTGCG
GCAACAGCCCGGATTGCAGCGGGTACGAGATCGAGCAGGGAAAATTCCGCATCAAGGGCTATGACGGGCCCACCATCGAA
TGCGACAAGTGCGGCAGCGAGATGCAGCTCAAGTCGGGACGTTTCGGCAAGTACTTCGGCTGCACCAACGAGAACTGCAA
GAACACGCGCAAGTTGCTGCGCAATGGCGAGGTCGCGCCGCCCAAGATGGATCCGATCCCGATGCCCGAGCTGGCCTGCC
AGAAGGTCGAGGACCATTACGTGTTGCGCGATGGCGCCAGCGGCCTGTTCCTGGCTGCGAGCCAGTTTCCCAAGAACCGG
GAAACGCGTGCGCCACTGGTCAAGGAGCTCAAGGCGCATCGCGACGAGTTGCCCGAGAAATATCATTTTCTGCTCGACGC
GCCGAGCGAGGACCCTGATGGCCGCGACGCCCAGATCCGCTTCTCGCGCAAGACCAAGCGCCAGTACGTGATGAGCGAGC
GCGACGGCAAGGCGAGCGGGTGGCGCGCCGAGTACGAGGATGGCCAATGGCGAGTCGAGGATAAGCGCAAATGA

Upstream 100 bases:

>100_bases
CGAGGCGCTAAGGTAGGGTAGGCGCATTTTTCGCCGGGCGGTTCCCGGCCACGACATTACTGCCGGCGCCATGCGGCGTC
GGCCATTCGAGTGGAGCGTT

Downstream 100 bases:

>100_bases
CACCGCGCGGGCGGACCAATCAACTGCTGTATCACGCCGAGCTGCTGCTCGGCGCCGAGGTCAAGGACGACGAGCACGCC
GCTGCCCGTTGCATGGCCCA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 877; Mature: 876

Protein sequence:

>877_residues
MGKSLVIVESPAKAKTINKYLGNDFVVKSSVGHIRDLPTSGSGKNATDPKERARQAAATRKMSPDEKAVYKKQKHWNQLV
RRMGIDPEHGWEANYEVLPGKEKVVAELKKLAEKADAVYLATDLDREGEAIAWHLQETIGGDASRYRRVVFNEITKNAIQ
EAFKTPGDLDLSRVEAQQARRFLDRVVGFMLSPLLWSKIARGLSAGRVQSVAVRLIVEREREIRAFVPEEFWDVHADLLP
ASGEDTPIRFEVARHQGKAFRPTSEQETLERIAPLRDAALTITERETKPTRSKPNAPFITSTLQQAASGRLGFSVKKTMT
LAQRLYEAGYITYMRTDSTNLSQDAVESARAFIGERFGQSYLPDAPNRYASKDGAQEAHEAIRPTEVTQAGDDLAVERDA
QRLYELIWRQFVACQMVPAEYLATTLTIEAEGFELKARGRVLKFDGYTRVMKPAGKKEEDQSLPDLEEGAEMTLQALDPQ
QHFTKPAPRYTEASLVKELEKRGIGRPSTYASIISTIQDRGYVKLENRRFYAEKLGDIVTDRLAESFSDLMDYGFTARME
DHLDEIAAGNREWRELLDAFYAEFKAKLEHAEAEEGMRPNQPVPTDIDCPTCGRKMQIRTASTGVFLGCSGYNLPPKERC
KTTIDLIAGDEAVAADADEEAETEALRAKRRCPECGTAMDSYLIDETRKLHICGNSPDCSGYEIEQGKFRIKGYDGPTIE
CDKCGSEMQLKSGRFGKYFGCTNENCKNTRKLLRNGEVAPPKMDPIPMPELACQKVEDHYVLRDGASGLFLAASQFPKNR
ETRAPLVKELKAHRDELPEKYHFLLDAPSEDPDGRDAQIRFSRKTKRQYVMSERDGKASGWRAEYEDGQWRVEDKRK

Sequences:

>Translated_877_residues
MGKSLVIVESPAKAKTINKYLGNDFVVKSSVGHIRDLPTSGSGKNATDPKERARQAAATRKMSPDEKAVYKKQKHWNQLV
RRMGIDPEHGWEANYEVLPGKEKVVAELKKLAEKADAVYLATDLDREGEAIAWHLQETIGGDASRYRRVVFNEITKNAIQ
EAFKTPGDLDLSRVEAQQARRFLDRVVGFMLSPLLWSKIARGLSAGRVQSVAVRLIVEREREIRAFVPEEFWDVHADLLP
ASGEDTPIRFEVARHQGKAFRPTSEQETLERIAPLRDAALTITERETKPTRSKPNAPFITSTLQQAASGRLGFSVKKTMT
LAQRLYEAGYITYMRTDSTNLSQDAVESARAFIGERFGQSYLPDAPNRYASKDGAQEAHEAIRPTEVTQAGDDLAVERDA
QRLYELIWRQFVACQMVPAEYLATTLTIEAEGFELKARGRVLKFDGYTRVMKPAGKKEEDQSLPDLEEGAEMTLQALDPQ
QHFTKPAPRYTEASLVKELEKRGIGRPSTYASIISTIQDRGYVKLENRRFYAEKLGDIVTDRLAESFSDLMDYGFTARME
DHLDEIAAGNREWRELLDAFYAEFKAKLEHAEAEEGMRPNQPVPTDIDCPTCGRKMQIRTASTGVFLGCSGYNLPPKERC
KTTIDLIAGDEAVAADADEEAETEALRAKRRCPECGTAMDSYLIDETRKLHICGNSPDCSGYEIEQGKFRIKGYDGPTIE
CDKCGSEMQLKSGRFGKYFGCTNENCKNTRKLLRNGEVAPPKMDPIPMPELACQKVEDHYVLRDGASGLFLAASQFPKNR
ETRAPLVKELKAHRDELPEKYHFLLDAPSEDPDGRDAQIRFSRKTKRQYVMSERDGKASGWRAEYEDGQWRVEDKRK
>Mature_876_residues
GKSLVIVESPAKAKTINKYLGNDFVVKSSVGHIRDLPTSGSGKNATDPKERARQAAATRKMSPDEKAVYKKQKHWNQLVR
RMGIDPEHGWEANYEVLPGKEKVVAELKKLAEKADAVYLATDLDREGEAIAWHLQETIGGDASRYRRVVFNEITKNAIQE
AFKTPGDLDLSRVEAQQARRFLDRVVGFMLSPLLWSKIARGLSAGRVQSVAVRLIVEREREIRAFVPEEFWDVHADLLPA
SGEDTPIRFEVARHQGKAFRPTSEQETLERIAPLRDAALTITERETKPTRSKPNAPFITSTLQQAASGRLGFSVKKTMTL
AQRLYEAGYITYMRTDSTNLSQDAVESARAFIGERFGQSYLPDAPNRYASKDGAQEAHEAIRPTEVTQAGDDLAVERDAQ
RLYELIWRQFVACQMVPAEYLATTLTIEAEGFELKARGRVLKFDGYTRVMKPAGKKEEDQSLPDLEEGAEMTLQALDPQQ
HFTKPAPRYTEASLVKELEKRGIGRPSTYASIISTIQDRGYVKLENRRFYAEKLGDIVTDRLAESFSDLMDYGFTARMED
HLDEIAAGNREWRELLDAFYAEFKAKLEHAEAEEGMRPNQPVPTDIDCPTCGRKMQIRTASTGVFLGCSGYNLPPKERCK
TTIDLIAGDEAVAADADEEAETEALRAKRRCPECGTAMDSYLIDETRKLHICGNSPDCSGYEIEQGKFRIKGYDGPTIEC
DKCGSEMQLKSGRFGKYFGCTNENCKNTRKLLRNGEVAPPKMDPIPMPELACQKVEDHYVLRDGASGLFLAASQFPKNRE
TRAPLVKELKAHRDELPEKYHFLLDAPSEDPDGRDAQIRFSRKTKRQYVMSERDGKASGWRAEYEDGQWRVEDKRK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=611, Percent_Identity=25.69558101473, Blast_Score=148, Evalue=3e-35,
Organism=Homo sapiens, GI4507635, Length=619, Percent_Identity=24.5557350565428, Blast_Score=136, Evalue=9e-32,
Organism=Escherichia coli, GI1787529, Length=880, Percent_Identity=66.9318181818182, Blast_Score=1220, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=654, Percent_Identity=22.782874617737, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32563869, Length=569, Percent_Identity=26.5377855887522, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17555378, Length=531, Percent_Identity=25.8003766478343, Blast_Score=122, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=477, Percent_Identity=25.5765199161426, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24585251, Length=629, Percent_Identity=25.4372019077901, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24640096, Length=506, Percent_Identity=27.8656126482213, Blast_Score=132, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98832; Mature: 98700

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLVIVESPAKAKTINKYLGNDFVVKSSVGHIRDLPTSGSGKNATDPKERARQAAATR
CCCCEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHH
KMSPDEKAVYKKQKHWNQLVRRMGIDPEHGWEANYEVLPGKEKVVAELKKLAEKADAVYL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCEEEE
ATDLDREGEAIAWHLQETIGGDASRYRRVVFNEITKNAIQEAFKTPGDLDLSRVEAQQAR
EECCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RFLDRVVGFMLSPLLWSKIARGLSAGRVQSVAVRLIVEREREIRAFVPEEFWDVHADLLP
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
ASGEDTPIRFEVARHQGKAFRPTSEQETLERIAPLRDAALTITERETKPTRSKPNAPFIT
CCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCHHH
STLQQAASGRLGFSVKKTMTLAQRLYEAGYITYMRTDSTNLSQDAVESARAFIGERFGQS
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
YLPDAPNRYASKDGAQEAHEAIRPTEVTQAGDDLAVERDAQRLYELIWRQFVACQMVPAE
CCCCCCCHHCCCCCHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
YLATTLTIEAEGFELKARGRVLKFDGYTRVMKPAGKKEEDQSLPDLEEGAEMTLQALDPQ
HHHEEEEEEECCEEEEECCCEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
QHFTKPAPRYTEASLVKELEKRGIGRPSTYASIISTIQDRGYVKLENRRFYAEKLGDIVT
HHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
DRLAESFSDLMDYGFTARMEDHLDEIAAGNREWRELLDAFYAEFKAKLEHAEAEEGMRPN
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QPVPTDIDCPTCGRKMQIRTASTGVFLGCSGYNLPPKERCKTTIDLIAGDEAVAADADEE
CCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHHHHHHHHHEECCCHHCCCCCHH
AETEALRAKRRCPECGTAMDSYLIDETRKLHICGNSPDCSGYEIEQGKFRIKGYDGPTIE
HHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCEEEEECCCCCCCC
CDKCGSEMQLKSGRFGKYFGCTNENCKNTRKLLRNGEVAPPKMDPIPMPELACQKVEDHY
HHHCCCCHHHCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCE
VLRDGASGLFLAASQFPKNRETRAPLVKELKAHRDELPEKYHFLLDAPSEDPDGRDAQIR
EEECCCCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCEEE
FSRKTKRQYVMSERDGKASGWRAEYEDGQWRVEDKRK
EHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCC
>Mature Secondary Structure 
GKSLVIVESPAKAKTINKYLGNDFVVKSSVGHIRDLPTSGSGKNATDPKERARQAAATR
CCCEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHH
KMSPDEKAVYKKQKHWNQLVRRMGIDPEHGWEANYEVLPGKEKVVAELKKLAEKADAVYL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCEEEE
ATDLDREGEAIAWHLQETIGGDASRYRRVVFNEITKNAIQEAFKTPGDLDLSRVEAQQAR
EECCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RFLDRVVGFMLSPLLWSKIARGLSAGRVQSVAVRLIVEREREIRAFVPEEFWDVHADLLP
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
ASGEDTPIRFEVARHQGKAFRPTSEQETLERIAPLRDAALTITERETKPTRSKPNAPFIT
CCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCHHH
STLQQAASGRLGFSVKKTMTLAQRLYEAGYITYMRTDSTNLSQDAVESARAFIGERFGQS
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
YLPDAPNRYASKDGAQEAHEAIRPTEVTQAGDDLAVERDAQRLYELIWRQFVACQMVPAE
CCCCCCCHHCCCCCHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
YLATTLTIEAEGFELKARGRVLKFDGYTRVMKPAGKKEEDQSLPDLEEGAEMTLQALDPQ
HHHEEEEEEECCEEEEECCCEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
QHFTKPAPRYTEASLVKELEKRGIGRPSTYASIISTIQDRGYVKLENRRFYAEKLGDIVT
HHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
DRLAESFSDLMDYGFTARMEDHLDEIAAGNREWRELLDAFYAEFKAKLEHAEAEEGMRPN
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QPVPTDIDCPTCGRKMQIRTASTGVFLGCSGYNLPPKERCKTTIDLIAGDEAVAADADEE
CCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHHHHHHHHHEECCCHHCCCCCHH
AETEALRAKRRCPECGTAMDSYLIDETRKLHICGNSPDCSGYEIEQGKFRIKGYDGPTIE
HHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCEEEEECCCCCCCC
CDKCGSEMQLKSGRFGKYFGCTNENCKNTRKLLRNGEVAPPKMDPIPMPELACQKVEDHY
HHHCCCCHHHCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCE
VLRDGASGLFLAASQFPKNRETRAPLVKELKAHRDELPEKYHFLLDAPSEDPDGRDAQIR
EEECCCCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCEEE
FSRKTKRQYVMSERDGKASGWRAEYEDGQWRVEDKRK
EHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]