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The map label for this gene is metZ [H]

Identifier: 92113394

GI number: 92113394

Start: 1446712

End: 1447920

Strand: Direct

Name: metZ [H]

Synonym: Csal_1268

Alternate gene names: 92113394

Gene position: 1446712-1447920 (Clockwise)

Preceding gene: 92113393

Following gene: 92113396

Centisome position: 39.14

GC content: 66.34

Gene sequence:

>1209_bases
ATGGCGGACGAGATCCACGAGGCACTCGCGCTCGACACGCTGGCGATCCGTGCCGGCCACGAGCGGACGCACGAACAGGA
ACACGGCGAGCCGATCTTTCCGACGTCGAGCTTCGTCTACGATAGCGCCGCCGAGGCGGCGCGCAAGTTCTCGGGCGAAG
AGCCGGGCAACATCTATTCACGGTTCACCAACCCCACGGTGCGTACCTTCGAGCAGCGTCTGGCGGCGCTGGAAGGCGGC
GAGCGCTGCGTGGCCACCAGCTCCGGTATGGCGGCGATTCTGTCCACGGCCCTGGCGTTCCTGCAGGCAGGCGATGAAAT
CGTCGCTTCGCGCTCGCTGTTCGGCTCGACGGTGAGCCTGTTCGACAAGTACCTCGGCAAGCTGGGGATCGTGACGCACT
ACGTGGAACTGGGCGATCTGGATGCCTGGGAAGCGGCCATCACTCCCAGCACACGCTTGTTGTTCGCCGAGACGCCTTCC
AACCCCTTGTCCGAGGTGGTGGACATCGCCGCGCTGGCCGAGATCGCCCATCGCCACGGGGCCTGGCTGGCCATCGACAA
CTGCTTTTTGACCCCGGCGCTGCAGCGGCCGCTGGACCTGGGGGCCGATCTGGTGATCCATTCGGCGACCAAGTATCTGG
ACGGCCAGGGCCGCGCCATCGGCGGTGCCGTGGTGGGCTCCAGCGAGATGCTTCAGGAACTGGTGGGCGTCGTGCGCACC
TGCGGGCCGTGCATGAGCCCCTTCAACGCCTGGATCTTCCTCAAGGGGCTGGAAACACTGAGCTTGCGCATGAAAGCCCA
TGACGAGAATGCGCAGGCCCTGGCGCGGTGGCTGGAGGCGCAGCCGGAGATTGCGCGCGTGCACTACAGCGGTCTGGAAG
GGCATCCCCAGCATGCGCTGGCCAAACGTCAGCAGCGCGGCTTCGGCGCCGTGCTGGGTGTTGAGGTCAAGGGAGGACGC
GACGGGGCCTGGACGCTGATCGACAACTCGCGCTTGATGTCGATCACAGGCAACCTGGGCGATGTGAAAACCACGGTCAC
GCATCCCGCGACGACGACCCACGCGCGCTTGAGCGACGCGCAGAAAGCCGCCGCGGGCATCAGTGAAGGCCTGATTCGCA
TCGCCGTGGGGCTGGAAGCGCTGGACGACCTCAAGCGTGATATCCGGCGTGGCCTGGACAGGCTCGCCGACGTGCCGGGC
ATCCGCTAG

Upstream 100 bases:

>100_bases
AGGCCGCGCGCAACGATCTGGCCAAGCATCGCGAAAACACGGTCCACGCCGTGGTCGACTTGCATAACGACGTCGAAGAA
GACAACTGAGGAGGCGAGGC

Downstream 100 bases:

>100_bases
TCCGGACGCCCGGCGTCACCCGTGCGCGTGCCCCGAACGCCTCGCCTCATGGCGAGGCGTTTTGCATGGCGTCAACGCGT
TGTCAGGCCATCGACGATAT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MADEIHEALALDTLAIRAGHERTHEQEHGEPIFPTSSFVYDSAAEAARKFSGEEPGNIYSRFTNPTVRTFEQRLAALEGG
ERCVATSSGMAAILSTALAFLQAGDEIVASRSLFGSTVSLFDKYLGKLGIVTHYVELGDLDAWEAAITPSTRLLFAETPS
NPLSEVVDIAALAEIAHRHGAWLAIDNCFLTPALQRPLDLGADLVIHSATKYLDGQGRAIGGAVVGSSEMLQELVGVVRT
CGPCMSPFNAWIFLKGLETLSLRMKAHDENAQALARWLEAQPEIARVHYSGLEGHPQHALAKRQQRGFGAVLGVEVKGGR
DGAWTLIDNSRLMSITGNLGDVKTTVTHPATTTHARLSDAQKAAAGISEGLIRIAVGLEALDDLKRDIRRGLDRLADVPG
IR

Sequences:

>Translated_402_residues
MADEIHEALALDTLAIRAGHERTHEQEHGEPIFPTSSFVYDSAAEAARKFSGEEPGNIYSRFTNPTVRTFEQRLAALEGG
ERCVATSSGMAAILSTALAFLQAGDEIVASRSLFGSTVSLFDKYLGKLGIVTHYVELGDLDAWEAAITPSTRLLFAETPS
NPLSEVVDIAALAEIAHRHGAWLAIDNCFLTPALQRPLDLGADLVIHSATKYLDGQGRAIGGAVVGSSEMLQELVGVVRT
CGPCMSPFNAWIFLKGLETLSLRMKAHDENAQALARWLEAQPEIARVHYSGLEGHPQHALAKRQQRGFGAVLGVEVKGGR
DGAWTLIDNSRLMSITGNLGDVKTTVTHPATTTHARLSDAQKAAAGISEGLIRIAVGLEALDDLKRDIRRGLDRLADVPG
IR
>Mature_401_residues
ADEIHEALALDTLAIRAGHERTHEQEHGEPIFPTSSFVYDSAAEAARKFSGEEPGNIYSRFTNPTVRTFEQRLAALEGGE
RCVATSSGMAAILSTALAFLQAGDEIVASRSLFGSTVSLFDKYLGKLGIVTHYVELGDLDAWEAAITPSTRLLFAETPSN
PLSEVVDIAALAEIAHRHGAWLAIDNCFLTPALQRPLDLGADLVIHSATKYLDGQGRAIGGAVVGSSEMLQELVGVVRTC
GPCMSPFNAWIFLKGLETLSLRMKAHDENAQALARWLEAQPEIARVHYSGLEGHPQHALAKRQQRGFGAVLGVEVKGGRD
GAWTLIDNSRLMSITGNLGDVKTTVTHPATTTHARLSDAQKAAAGISEGLIRIAVGLEALDDLKRDIRRGLDRLADVPGI
R

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=37.5, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=35.0609756097561, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=31.1926605504587, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI1790375, Length=386, Percent_Identity=37.0466321243523, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789383, Length=406, Percent_Identity=28.3251231527094, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17560056, Length=353, Percent_Identity=37.3937677053824, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=375, Percent_Identity=35.2, Blast_Score=228, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=375, Percent_Identity=30.4, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=34.9425287356322, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6319307, Length=396, Percent_Identity=32.8282828282828, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=403, Percent_Identity=27.2952853598015, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321170, Length=307, Percent_Identity=26.3843648208469, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=379, Percent_Identity=26.3852242744063, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922554, Length=345, Percent_Identity=35.6521739130435, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43200; Mature: 43069

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEIHEALALDTLAIRAGHERTHEQEHGEPIFPTSSFVYDSAAEAARKFSGEEPGNIYS
CCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
RFTNPTVRTFEQRLAALEGGERCVATSSGMAAILSTALAFLQAGDEIVASRSLFGSTVSL
HCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FDKYLGKLGIVTHYVELGDLDAWEAAITPSTRLLFAETPSNPLSEVVDIAALAEIAHRHG
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
AWLAIDNCFLTPALQRPLDLGADLVIHSATKYLDGQGRAIGGAVVGSSEMLQELVGVVRT
CEEEECCEECCHHHHCCHHCCCHHHHHHHHHHHCCCCCEECCEEECCHHHHHHHHHHHHH
CGPCMSPFNAWIFLKGLETLSLRMKAHDENAQALARWLEAQPEIARVHYSGLEGHPQHAL
CCHHCCCHHHEEEEECHHHHEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
AKRQQRGFGAVLGVEVKGGRDGAWTLIDNSRLMSITGNLGDVKTTVTHPATTTHARLSDA
HHHHHCCCCEEEEEEEECCCCCEEEEEECCEEEEEECCCCCCEEEEECCCCCHHHHHHHH
QKAAAGISEGLIRIAVGLEALDDLKRDIRRGLDRLADVPGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADEIHEALALDTLAIRAGHERTHEQEHGEPIFPTSSFVYDSAAEAARKFSGEEPGNIYS
CHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
RFTNPTVRTFEQRLAALEGGERCVATSSGMAAILSTALAFLQAGDEIVASRSLFGSTVSL
HCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FDKYLGKLGIVTHYVELGDLDAWEAAITPSTRLLFAETPSNPLSEVVDIAALAEIAHRHG
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
AWLAIDNCFLTPALQRPLDLGADLVIHSATKYLDGQGRAIGGAVVGSSEMLQELVGVVRT
CEEEECCEECCHHHHCCHHCCCHHHHHHHHHHHCCCCCEECCEEECCHHHHHHHHHHHHH
CGPCMSPFNAWIFLKGLETLSLRMKAHDENAQALARWLEAQPEIARVHYSGLEGHPQHAL
CCHHCCCHHHEEEEECHHHHEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
AKRQQRGFGAVLGVEVKGGRDGAWTLIDNSRLMSITGNLGDVKTTVTHPATTTHARLSDA
HHHHHCCCCEEEEEEEECCCCCEEEEEECCEEEEEECCCCCCEEEEECCCCCHHHHHHHH
QKAAAGISEGLIRIAVGLEALDDLKRDIRRGLDRLADVPGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]