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The map label for this gene is purF [H]

Identifier: 92113393

GI number: 92113393

Start: 1445180

End: 1446700

Strand: Direct

Name: purF [H]

Synonym: Csal_1267

Alternate gene names: 92113393

Gene position: 1445180-1446700 (Clockwise)

Preceding gene: 92113392

Following gene: 92113394

Centisome position: 39.09

GC content: 63.84

Gene sequence:

>1521_bases
ATGTGCGGTATCGTGGGCCTCATGGCCAATCAGGCGGTCAATCAGTCGCTGTATGACGCGCTGACAGTGCTCCAACACCG
TGGCCAGGATGCCGCCGGGATGATGACATGGCATGAGGGACGCTTCCTGTTGCGCAAGAGCAACGGGCTGGTGCGGGATG
TCTTCCACACGCGTCACATGACGCGCCTGCGCGGCAACATGGGCATCGGGCATATCCGCTATCCCACCGCGGGCTCGTCG
AGCGAAGCGGAGTCGCAGCCGTTCTACGTCAATTCGCCCTACGGGATCTCCCTGGCGCACAACGGCAACCTGACCAACTC
CGAACAGCTCAAGCGCGAGCTGTTCTTTTCCGACCTGCGCCACATCAACACCAGCTCCGACTCGGAAGTGCTGCTCAACG
TCTTCGCGCACGAGCTCGGCAAGCAGGGACATCACCTGACACCCGACGATATCTTCGATGCGGTGCGTCGGGTGCACCGC
CGTTGCCAAGGGGGCTACGCCGCGGTGGCCTTCATCAACGGTTTCGGCATGGTGGCGTTTCGCGACCCCCATGGCATCCG
CCCGGTGGTCTACGGCACGCGCGACACCGAGGAAGGCCAGGAGGTGATGATCGCCTCCGAATCGGTGGCGCTGGACGTGG
GGGGCTTCGAGCTGTGCCGCGACCTGGCGCCGGGCGAGGCGATCTTCATCGAAACCGATGGCAACCGCGTGCACACCCAG
CAGTGTGCCGATCGCCCCCGCCTGACACCCTGCATCTTCGAGCACGTCTACCTGGCGCGTCCCGATTCGATCCTCGACGG
CGCCTATGTCTACGGCACGCGCATGGAAATGGGGCGCAAGCTGGGCGATCGCATCCTGCGCGACTGGCCGGAACATGACA
TCGACGTGGTGATCCCGATCCCCGACACCTCGCGTACCTCGGCGCTGGAACTGGCGCAGCACCTGGGCGTCACCTATCGC
GAAGGCTTCATGAAGAACCGCTACATCGGGCGGACCTTCATCATGCCCGGCCAGACCCAGCGCAAGAAATCGGTGCGCCA
GAAGCTCAACGCCATCGACGTCGAGTTTCAGGGCAAGAACGTGTTGCTGGTCGACGACTCCATCGTGCGCGGCACGACCT
GCAAGCAGATCATCCAGATGGCGCGCGAGGCCGGCGCGCGCAAGGTGTATTTCGCCTCCGCCGCGCCTCCGGTGCGCTAT
CCCAATGTCTACGGCATCGACATGCCGGTCGCCTCGGAACTGATCGCTCACGGGCGCAGCGACGAGGAAGTCGGCGAGCT
GATCGGTGCCGACCGCATCGTCTATCAGGAGCTCGAGGACCTCAAGGCCGCATGCCGCGACGTCAATCCGACCCTCGAGG
AATTCGACTGCTCGGTGTTCGACGGCCAGTACGTGACCGGAGACATCGATGCGGCCTACCTGGCCGACCTCGAGGCCGCG
CGCAACGATCTGGCCAAGCATCGCGAAAACACGGTCCACGCCGTGGTCGACTTGCATAACGACGTCGAAGAAGACAACTG
A

Upstream 100 bases:

>100_bases
GGGCGCCGTGAGACGGCGCGCATGTATCACAGGTTTTTGACGCGAGCGACGCGGTTCGGCGATCGCTCGCCACTCCCAAG
GCAAGCGAGGTAAAGGCGCA

Downstream 100 bases:

>100_bases
GGAGGCGAGGCATGGCGGACGAGATCCACGAGGCACTCGCGCTCGACACGCTGGCGATCCGTGCCGGCCACGAGCGGACG
CACGAACAGGAACACGGCGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MCGIVGLMANQAVNQSLYDALTVLQHRGQDAAGMMTWHEGRFLLRKSNGLVRDVFHTRHMTRLRGNMGIGHIRYPTAGSS
SEAESQPFYVNSPYGISLAHNGNLTNSEQLKRELFFSDLRHINTSSDSEVLLNVFAHELGKQGHHLTPDDIFDAVRRVHR
RCQGGYAAVAFINGFGMVAFRDPHGIRPVVYGTRDTEEGQEVMIASESVALDVGGFELCRDLAPGEAIFIETDGNRVHTQ
QCADRPRLTPCIFEHVYLARPDSILDGAYVYGTRMEMGRKLGDRILRDWPEHDIDVVIPIPDTSRTSALELAQHLGVTYR
EGFMKNRYIGRTFIMPGQTQRKKSVRQKLNAIDVEFQGKNVLLVDDSIVRGTTCKQIIQMAREAGARKVYFASAAPPVRY
PNVYGIDMPVASELIAHGRSDEEVGELIGADRIVYQELEDLKAACRDVNPTLEEFDCSVFDGQYVTGDIDAAYLADLEAA
RNDLAKHRENTVHAVVDLHNDVEEDN

Sequences:

>Translated_506_residues
MCGIVGLMANQAVNQSLYDALTVLQHRGQDAAGMMTWHEGRFLLRKSNGLVRDVFHTRHMTRLRGNMGIGHIRYPTAGSS
SEAESQPFYVNSPYGISLAHNGNLTNSEQLKRELFFSDLRHINTSSDSEVLLNVFAHELGKQGHHLTPDDIFDAVRRVHR
RCQGGYAAVAFINGFGMVAFRDPHGIRPVVYGTRDTEEGQEVMIASESVALDVGGFELCRDLAPGEAIFIETDGNRVHTQ
QCADRPRLTPCIFEHVYLARPDSILDGAYVYGTRMEMGRKLGDRILRDWPEHDIDVVIPIPDTSRTSALELAQHLGVTYR
EGFMKNRYIGRTFIMPGQTQRKKSVRQKLNAIDVEFQGKNVLLVDDSIVRGTTCKQIIQMAREAGARKVYFASAAPPVRY
PNVYGIDMPVASELIAHGRSDEEVGELIGADRIVYQELEDLKAACRDVNPTLEEFDCSVFDGQYVTGDIDAAYLADLEAA
RNDLAKHRENTVHAVVDLHNDVEEDN
>Mature_506_residues
MCGIVGLMANQAVNQSLYDALTVLQHRGQDAAGMMTWHEGRFLLRKSNGLVRDVFHTRHMTRLRGNMGIGHIRYPTAGSS
SEAESQPFYVNSPYGISLAHNGNLTNSEQLKRELFFSDLRHINTSSDSEVLLNVFAHELGKQGHHLTPDDIFDAVRRVHR
RCQGGYAAVAFINGFGMVAFRDPHGIRPVVYGTRDTEEGQEVMIASESVALDVGGFELCRDLAPGEAIFIETDGNRVHTQ
QCADRPRLTPCIFEHVYLARPDSILDGAYVYGTRMEMGRKLGDRILRDWPEHDIDVVIPIPDTSRTSALELAQHLGVTYR
EGFMKNRYIGRTFIMPGQTQRKKSVRQKLNAIDVEFQGKNVLLVDDSIVRGTTCKQIIQMAREAGARKVYFASAAPPVRY
PNVYGIDMPVASELIAHGRSDEEVGELIGADRIVYQELEDLKAACRDVNPTLEEFDCSVFDGQYVTGDIDAAYLADLEAA
RNDLAKHRENTVHAVVDLHNDVEEDN

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=476, Percent_Identity=34.0336134453782, Blast_Score=248, Evalue=1e-65,
Organism=Escherichia coli, GI1788651, Length=506, Percent_Identity=62.0553359683794, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=170, Percent_Identity=32.3529411764706, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=454, Percent_Identity=32.3788546255507, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6323958, Length=506, Percent_Identity=51.9762845849802, Blast_Score=507, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24659598, Length=477, Percent_Identity=33.5429769392034, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24659604, Length=469, Percent_Identity=33.9019189765458, Blast_Score=248, Evalue=9e-66,
Organism=Drosophila melanogaster, GI28573187, Length=478, Percent_Identity=34.3096234309623, Blast_Score=247, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56531; Mature: 56531

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGLMANQAVNQSLYDALTVLQHRGQDAAGMMTWHEGRFLLRKSNGLVRDVFHTRHM
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHH
TRLRGNMGIGHIRYPTAGSSSEAESQPFYVNSPYGISLAHNGNLTNSEQLKRELFFSDLR
HHHHCCCCCCEEECCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHH
HINTSSDSEVLLNVFAHELGKQGHHLTPDDIFDAVRRVHRRCQGGYAAVAFINGFGMVAF
HCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEE
RDPHGIRPVVYGTRDTEEGQEVMIASESVALDVGGFELCRDLAPGEAIFIETDGNRVHTQ
ECCCCCCCEEECCCCCCCCCEEEEEECCEEEECCHHHHHHHCCCCCEEEEEECCCEEEHH
QCADRPRLTPCIFEHVYLARPDSILDGAYVYGTRMEMGRKLGDRILRDWPEHDIDVVIPI
HHCCCCCCCHHHHHHHHHCCCCCHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PDTSRTSALELAQHLGVTYREGFMKNRYIGRTFIMPGQTQRKKSVRQKLNAIDVEFQGKN
CCCCCHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCEEEEECCCE
VLLVDDSIVRGTTCKQIIQMAREAGARKVYFASAAPPVRYPNVYGIDMPVASELIAHGRS
EEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHCCCC
DEEVGELIGADRIVYQELEDLKAACRDVNPTLEEFDCSVFDGQYVTGDIDAAYLADLEAA
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEECCEEEECCCCHHHHHHHHHH
RNDLAKHRENTVHAVVDLHNDVEEDN
HHHHHHHHCCCEEEEEEHHHHCCCCC
>Mature Secondary Structure
MCGIVGLMANQAVNQSLYDALTVLQHRGQDAAGMMTWHEGRFLLRKSNGLVRDVFHTRHM
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHH
TRLRGNMGIGHIRYPTAGSSSEAESQPFYVNSPYGISLAHNGNLTNSEQLKRELFFSDLR
HHHHCCCCCCEEECCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHH
HINTSSDSEVLLNVFAHELGKQGHHLTPDDIFDAVRRVHRRCQGGYAAVAFINGFGMVAF
HCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEE
RDPHGIRPVVYGTRDTEEGQEVMIASESVALDVGGFELCRDLAPGEAIFIETDGNRVHTQ
ECCCCCCCEEECCCCCCCCCEEEEEECCEEEECCHHHHHHHCCCCCEEEEEECCCEEEHH
QCADRPRLTPCIFEHVYLARPDSILDGAYVYGTRMEMGRKLGDRILRDWPEHDIDVVIPI
HHCCCCCCCHHHHHHHHHCCCCCHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PDTSRTSALELAQHLGVTYREGFMKNRYIGRTFIMPGQTQRKKSVRQKLNAIDVEFQGKN
CCCCCHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCEEEEECCCE
VLLVDDSIVRGTTCKQIIQMAREAGARKVYFASAAPPVRYPNVYGIDMPVASELIAHGRS
EEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHCCCC
DEEVGELIGADRIVYQELEDLKAACRDVNPTLEEFDCSVFDGQYVTGDIDAAYLADLEAA
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEECCEEEECCCCHHHHHHHHHH
RNDLAKHRENTVHAVVDLHNDVEEDN
HHHHHHHHCCCEEEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]